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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Skeletal Proteome of the Coral <italic>Acropora millepora</italic>: The Evolution of Calcification by Co-Option and Domain Shuffling</title><author><name sortKey="Ramos Silva, Paula" sort="Ramos Silva, Paula" uniqKey="Ramos Silva P" first="Paula" last="Ramos-Silva">Paula Ramos-Silva</name><affiliation><nlm:aff id="mst109-AFF1">UMR 6282 CNRS, Biogéosciences, Université de Bourgogne, Dijon, France</nlm:aff></affiliation><affiliation><nlm:aff id="mst109-AFF2">Computational Science Section, Informatics Institute, Universiteit van Amsterdam, Amsterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Kaandorp, Jaap" sort="Kaandorp, Jaap" uniqKey="Kaandorp J" first="Jaap" last="Kaandorp">Jaap Kaandorp</name><affiliation><nlm:aff id="mst109-AFF2">Computational Science Section, Informatics Institute, Universiteit van Amsterdam, Amsterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Huisman, Lotte" sort="Huisman, Lotte" uniqKey="Huisman L" first="Lotte" last="Huisman">Lotte Huisman</name><affiliation><nlm:aff id="mst109-AFF2">Computational Science Section, Informatics Institute, Universiteit van Amsterdam, Amsterdam, The Netherlands</nlm:aff></affiliation><affiliation><nlm:aff id="mst109-AFF3">ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia</nlm:aff></affiliation></author><author><name sortKey="Marie, Benjamin" sort="Marie, Benjamin" uniqKey="Marie B" first="Benjamin" last="Marie">Benjamin Marie</name><affiliation><nlm:aff id="mst109-AFF4">UMR 7245 CNRS, MCAM, Muséum National d’Histoire Naturelle (MNHN), Paris, France</nlm:aff></affiliation></author><author><name sortKey="Zanella Cleon, Isabelle" sort="Zanella Cleon, Isabelle" uniqKey="Zanella Cleon I" first="Isabelle" last="Zanella-Cléon">Isabelle Zanella-Cléon</name><affiliation><nlm:aff id="mst109-AFF5">IFR 128 BioSciences Gerland-Lyon Sud, UMR 5086 CNRS, Institut de Biologie et Chimie des Protéines, Université de Lyon, Lyon, France</nlm:aff></affiliation></author><author><name sortKey="Guichard, Nathalie" sort="Guichard, Nathalie" uniqKey="Guichard N" first="Nathalie" last="Guichard">Nathalie Guichard</name><affiliation><nlm:aff id="mst109-AFF1">UMR 6282 CNRS, Biogéosciences, Université de Bourgogne, Dijon, France</nlm:aff></affiliation></author><author><name sortKey="Miller, David J" sort="Miller, David J" uniqKey="Miller D" first="David J." last="Miller">David J. Miller</name><affiliation><nlm:aff id="mst109-AFF3">ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia</nlm:aff></affiliation></author><author><name sortKey="Marin, Frederic" sort="Marin, Frederic" uniqKey="Marin F" first="Frédéric" last="Marin">Frédéric Marin</name><affiliation><nlm:aff id="mst109-AFF1">UMR 6282 CNRS, Biogéosciences, Université de Bourgogne, Dijon, France</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23765379</idno><idno type="pmc">3748352</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748352</idno><idno type="RBID">PMC:3748352</idno><idno type="doi">10.1093/molbev/mst109</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">002764</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002764</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The Skeletal Proteome of the Coral <italic>Acropora millepora</italic>: The Evolution of Calcification by Co-Option and Domain Shuffling</title><author><name sortKey="Ramos Silva, Paula" sort="Ramos Silva, Paula" uniqKey="Ramos Silva P" first="Paula" last="Ramos-Silva">Paula Ramos-Silva</name><affiliation><nlm:aff id="mst109-AFF1">UMR 6282 CNRS, Biogéosciences, Université de Bourgogne, Dijon, France</nlm:aff></affiliation><affiliation><nlm:aff id="mst109-AFF2">Computational Science Section, Informatics Institute, Universiteit van Amsterdam, Amsterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Kaandorp, Jaap" sort="Kaandorp, Jaap" uniqKey="Kaandorp J" first="Jaap" last="Kaandorp">Jaap Kaandorp</name><affiliation><nlm:aff id="mst109-AFF2">Computational Science Section, Informatics Institute, Universiteit van Amsterdam, Amsterdam, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Huisman, Lotte" sort="Huisman, Lotte" uniqKey="Huisman L" first="Lotte" last="Huisman">Lotte Huisman</name><affiliation><nlm:aff id="mst109-AFF2">Computational Science Section, Informatics Institute, Universiteit van Amsterdam, Amsterdam, The Netherlands</nlm:aff></affiliation><affiliation><nlm:aff id="mst109-AFF3">ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia</nlm:aff></affiliation></author><author><name sortKey="Marie, Benjamin" sort="Marie, Benjamin" uniqKey="Marie B" first="Benjamin" last="Marie">Benjamin Marie</name><affiliation><nlm:aff id="mst109-AFF4">UMR 7245 CNRS, MCAM, Muséum National d’Histoire Naturelle (MNHN), Paris, France</nlm:aff></affiliation></author><author><name sortKey="Zanella Cleon, Isabelle" sort="Zanella Cleon, Isabelle" uniqKey="Zanella Cleon I" first="Isabelle" last="Zanella-Cléon">Isabelle Zanella-Cléon</name><affiliation><nlm:aff id="mst109-AFF5">IFR 128 BioSciences Gerland-Lyon Sud, UMR 5086 CNRS, Institut de Biologie et Chimie des Protéines, Université de Lyon, Lyon, France</nlm:aff></affiliation></author><author><name sortKey="Guichard, Nathalie" sort="Guichard, Nathalie" uniqKey="Guichard N" first="Nathalie" last="Guichard">Nathalie Guichard</name><affiliation><nlm:aff id="mst109-AFF1">UMR 6282 CNRS, Biogéosciences, Université de Bourgogne, Dijon, France</nlm:aff></affiliation></author><author><name sortKey="Miller, David J" sort="Miller, David J" uniqKey="Miller D" first="David J." last="Miller">David J. Miller</name><affiliation><nlm:aff id="mst109-AFF3">ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia</nlm:aff></affiliation></author><author><name sortKey="Marin, Frederic" sort="Marin, Frederic" uniqKey="Marin F" first="Frédéric" last="Marin">Frédéric Marin</name><affiliation><nlm:aff id="mst109-AFF1">UMR 6282 CNRS, Biogéosciences, Université de Bourgogne, Dijon, France</nlm:aff></affiliation></author></analytic><series><title level="j">Molecular Biology and Evolution</title><idno type="ISSN">0737-4038</idno><idno type="eISSN">1537-1719</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>In corals, biocalcification is a major function that may be drastically affected by ocean acidification (OA). Scleractinian corals grow by building up aragonitic exoskeletons that provide support and protection for soft tissues. Although this process has been extensively studied, the molecular basis of biocalcification is poorly understood. Notably lacking is a comprehensive catalog of the skeleton-occluded proteins—the skeletal organic matrix proteins (SOMPs) that are thought to regulate the mineral deposition. Using a combination of proteomics and transcriptomics, we report the first survey of such proteins in the staghorn coral <italic>Acropora millepora</italic>. The organic matrix (OM) extracted from the coral skeleton was analyzed by mass spectrometry and bioinformatics, enabling the identification of 36 SOMPs. These results provide novel insights into the molecular basis of coral calcification and the macroevolution of metazoan calcifying systems, whereas establishing a platform for studying the impact of OA at molecular level. Besides secreted proteins, extracellular regions of transmembrane proteins are also present, suggesting a close control of aragonite deposition by the calicoblastic epithelium. In addition to the expected SOMPs (Asp/Glu-rich, galaxins), the skeletal repertoire included several proteins containing known extracellular matrix domains. From an evolutionary perspective, the number of coral-specific proteins is low, many SOMPs having counterparts in the noncalcifying cnidarians. Extending the comparison with the skeletal OM proteomes of other metazoans allowed the identification of a pool of functional domains shared between phyla. These data suggest that co-option and domain shuffling may be general mechanisms by which the trait of calcification has evolved.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Addadi, L" uniqKey="Addadi L">L Addadi</name></author><author><name sortKey="Moradian, J" uniqKey="Moradian J">J Moradian</name></author><author><name sortKey="Shay, E" uniqKey="Shay E">E Shay</name></author><author><name sortKey="Maroudas, Ng" uniqKey="Maroudas N">NG Maroudas</name></author><author><name sortKey="Weiner, S" uniqKey="Weiner S">S Weiner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Allemand, D" uniqKey="Allemand D">D Allemand</name></author><author><name sortKey="Tambutte, E" uniqKey="Tambutte E">É Tambutté</name></author><author><name sortKey="Zoccola, D" uniqKey="Zoccola D">D Zoccola</name></author><author><name sortKey="Tambutte, S" uniqKey="Tambutte S">S 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Mol Biol Evol</journal-id><journal-id journal-id-type="iso-abbrev">Mol. Biol. Evol</journal-id><journal-id journal-id-type="publisher-id">molbev</journal-id><journal-id journal-id-type="hwp">molbiolevol</journal-id><journal-title-group><journal-title>Molecular Biology and Evolution</journal-title></journal-title-group><issn pub-type="ppub">0737-4038</issn><issn pub-type="epub">1537-1719</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23765379</article-id><article-id pub-id-type="pmc">3748352</article-id><article-id pub-id-type="doi">10.1093/molbev/mst109</article-id><article-id pub-id-type="publisher-id">mst109</article-id><article-categories><subj-group subj-group-type="heading"><subject>Discoveries</subject></subj-group></article-categories><title-group><article-title>The Skeletal Proteome of the Coral <italic>Acropora millepora</italic>: The Evolution of Calcification by Co-Option and Domain Shuffling</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Ramos-Silva</surname><given-names>Paula</given-names></name><xref ref-type="aff" rid="mst109-AFF1"><sup>1</sup></xref><xref ref-type="aff" rid="mst109-AFF2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Kaandorp</surname><given-names>Jaap</given-names></name><xref ref-type="corresp" rid="mst109-COR1">*</xref><xref ref-type="aff" rid="mst109-AFF2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Huisman</surname><given-names>Lotte</given-names></name><xref ref-type="aff" rid="mst109-AFF2"><sup>2</sup></xref><xref ref-type="aff" rid="mst109-AFF3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Marie</surname><given-names>Benjamin</given-names></name><xref ref-type="aff" rid="mst109-AFF4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zanella-Cléon</surname><given-names>Isabelle</given-names></name><xref ref-type="aff" rid="mst109-AFF5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Guichard</surname><given-names>Nathalie</given-names></name><xref ref-type="aff" rid="mst109-AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Miller</surname><given-names>David J.</given-names></name><xref ref-type="aff" rid="mst109-AFF3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Marin</surname><given-names>Frédéric</given-names></name><xref ref-type="corresp" rid="mst109-COR1">*</xref><xref ref-type="aff" rid="mst109-AFF1"><sup>1</sup></xref></contrib></contrib-group><aff id="mst109-AFF1"><sup>1</sup>UMR 6282 CNRS, Biogéosciences, Université de Bourgogne, Dijon, France</aff><aff id="mst109-AFF2"><sup>2</sup>Computational Science Section, Informatics Institute, Universiteit van Amsterdam, Amsterdam, The Netherlands</aff><aff id="mst109-AFF3"><sup>3</sup>ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia</aff><aff id="mst109-AFF4"><sup>4</sup>UMR 7245 CNRS, MCAM, Muséum National d’Histoire Naturelle (MNHN), Paris, France</aff><aff id="mst109-AFF5"><sup>5</sup>IFR 128 BioSciences Gerland-Lyon Sud, UMR 5086 CNRS, Institut de Biologie et Chimie des Protéines, Université de Lyon, Lyon, France</aff><author-notes><corresp id="mst109-COR1"><bold>*Corresponding author:</bold> E-mail: <email>j.a.kaandorp@uva.nl</email>; <email>frederic.marin@u-bourgogne.fr</email>.</corresp><fn id="mst109-FN1"><p><bold>Associate editor:</bold> Charles Delwiche</p></fn><fn id="mst109-FN2"><p><bold>Data deposition:</bold> Protein sequence data reported in this article have been deposited in the UniProt Knowledgebase (accession nos. B3EWY[6-9], B3EWZ[0-9], B3EX0[0-2], B7W112, B7W114, B7WFQ1, B8RJM0, B8UU51, B8UU59, G8HTB6 B8UU74, D9IQ16, B8UU78, B8V7P3, B8V7Q1, B8V7R6, B8V7S0, B7T7N1, B8VIV4, B8VIU6, B8VIW9, B8VIX3, and B8WI85).</p></fn></author-notes><pub-date pub-type="ppub"><month>9</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>12</day><month>6</month><year>2013</year></pub-date><pub-date pub-type="pmc-release"><day>12</day><month>6</month><year>2013</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>30</volume><issue>9</issue><fpage>2099</fpage><lpage>2112</lpage><permissions><copyright-statement>© The Author 2013. Published by Oxford University Press.</copyright-statement><copyright-year>2013</copyright-year><license license-type="creative-commons" xlink:href="http://creativecommons.org/licenses/by-nc/3.0"><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</ext-link>), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com</license-p></license></permissions><abstract><p>In corals, biocalcification is a major function that may be drastically affected by ocean acidification (OA). Scleractinian corals grow by building up aragonitic exoskeletons that provide support and protection for soft tissues. Although this process has been extensively studied, the molecular basis of biocalcification is poorly understood. Notably lacking is a comprehensive catalog of the skeleton-occluded proteins—the skeletal organic matrix proteins (SOMPs) that are thought to regulate the mineral deposition. Using a combination of proteomics and transcriptomics, we report the first survey of such proteins in the staghorn coral <italic>Acropora millepora</italic>. The organic matrix (OM) extracted from the coral skeleton was analyzed by mass spectrometry and bioinformatics, enabling the identification of 36 SOMPs. These results provide novel insights into the molecular basis of coral calcification and the macroevolution of metazoan calcifying systems, whereas establishing a platform for studying the impact of OA at molecular level. Besides secreted proteins, extracellular regions of transmembrane proteins are also present, suggesting a close control of aragonite deposition by the calicoblastic epithelium. In addition to the expected SOMPs (Asp/Glu-rich, galaxins), the skeletal repertoire included several proteins containing known extracellular matrix domains. From an evolutionary perspective, the number of coral-specific proteins is low, many SOMPs having counterparts in the noncalcifying cnidarians. Extending the comparison with the skeletal OM proteomes of other metazoans allowed the identification of a pool of functional domains shared between phyla. These data suggest that co-option and domain shuffling may be general mechanisms by which the trait of calcification has evolved.</p></abstract><kwd-group><kwd>biomineralization</kwd><kwd>calcium carbonate skeleton</kwd><kwd>scleractinian</kwd><kwd>proteomics</kwd><kwd>evolution</kwd></kwd-group><counts><page-count count="14"></page-count></counts></article-meta></front><body><sec><title>Introduction</title><p>It is generally accepted that anthropogenic CO<sub>2</sub> emissions cause deleterious effects, not only on the atmosphere (i.e.<italic>,</italic> global warming) but also on seawater chemistry. Since pre-industrial times, the average pH of seawater has fallen from 8.2 to 8.1, and this ocean acidification (OA) is thought to have significantly affected marine organisms in a variety of ways (<xref ref-type="bibr" rid="mst109-B10">Caldeira and Wickett 2003</xref>; <xref ref-type="bibr" rid="mst109-B49">Kline et al. 2012</xref>), including impacts on important physiological functions such as calcification (<xref ref-type="bibr" rid="mst109-B76">Orr et al. 2005</xref>; <xref ref-type="bibr" rid="mst109-B13">Cohen et al. 2009</xref>; <xref ref-type="bibr" rid="mst109-B50">Kroeker et al. 2013</xref>). This process is essential for a wide range of marine metazoans and fulfils a diverse array of important physiological and ecological roles (<xref ref-type="bibr" rid="mst109-B19">Cusack and Freer 2008</xref>). In the neritic domain of tropical and subtropical regions, the most prominent calcium carbonate producers are cnidarians, in particular scleractinians (stony corals), where coral reefs have an annual net production ranging from 5 to 126 mol CaCO<sub>3</sub> m<sup>−</sup><sup>2</sup> year<sup>−</sup><sup>1</sup> (<xref ref-type="bibr" rid="mst109-B69">Milliman and Droxler 1996</xref>; <xref ref-type="bibr" rid="mst109-B32">Gattuso et al. 1998</xref>). Recent studies have shown that OA and increasing sea surface temperatures decrease coral calcification rates (<xref ref-type="bibr" rid="mst109-B12">Cohen and Mcconnaughey 2003</xref>; <xref ref-type="bibr" rid="mst109-B53">Langdon and Atkinson 2005</xref>; <xref ref-type="bibr" rid="mst109-B76">Orr et al. 2005</xref>; <xref ref-type="bibr" rid="mst109-B21">De’ath et al. 2009</xref>; <xref ref-type="bibr" rid="mst109-B48">Kleypas and Yates 2009</xref>; <xref ref-type="bibr" rid="mst109-B77">Pandolfi et al. 2011</xref>; <xref ref-type="bibr" rid="mst109-B11">Chan and Connolly 2013</xref>). However, most of the data supporting these negative effects are obtained at the organism/colony level and only few studies have considered the underlying molecular and cellular mechanisms. Of particular significance is the fact that the molecular machinery of coral biomineralization (the “calcification toolkit”) is, as yet, poorly characterized. Thus, a better understanding of the process is required, in particular at the level of the mineralizing space, where CaCO<sub>3</sub> crystals are formed and shaped (<xref ref-type="bibr" rid="mst109-B2">Allemand et al. 2011</xref>). A number of candidate genes for roles in coral calcification have been identified for being expressed in the calicoblastic ectoderm (<xref ref-type="bibr" rid="mst109-B104">Zoccola et al. 2004</xref>, <xref ref-type="bibr" rid="mst109-B103">2009</xref>; <xref ref-type="bibr" rid="mst109-B75">Moya et al. 2008</xref>). This number was recently increased with high-throughput analyses (<xref ref-type="bibr" rid="mst109-B67">Meyer et al. 2011</xref>; <xref ref-type="bibr" rid="mst109-B87">Shinzato et al. 2011</xref>; <xref ref-type="bibr" rid="mst109-B74">Moya et al. 2012</xref>), whereas microarray studies led to the identification of the SCRiPs, a family of small cysteine-rich proteins showing molecular features suggestive of an involvement in calcification processes (<xref ref-type="bibr" rid="mst109-B90">Sunagawa et al. 2009</xref>). Amongst the most obvious candidates for roles in coral calcification are the genes that encode skeletal organic matrix proteins (SOMPs), that is<italic>,</italic> proteins secreted by the calcifying tissues—the calicoblastic ectoderm in corals—which are occluded in the skeleton during its formation (<xref ref-type="bibr" rid="mst109-B92">Tambutté et al. 2008</xref>). These proteins are the main components of the skeletal organic matrix (OM), which in corals contains also polysaccharides (<xref ref-type="bibr" rid="mst109-B38">Goldberg 2001</xref>), glycoproteins (<xref ref-type="bibr" rid="mst109-B20">Dauphin and Cuif 1997</xref>), and lipids (<xref ref-type="bibr" rid="mst109-B27">Farre et al. 2010</xref>). The SOMPs have been characterized biochemically “in bulk” (i.e., without fractionation) in different coral species (<xref ref-type="bibr" rid="mst109-B17">Cuif, Dauphin, Gautret 1999</xref>; <xref ref-type="bibr" rid="mst109-B18">Cuif, Dauphin, Freiwald, et al. 1999</xref>; <xref ref-type="bibr" rid="mst109-B41">Ingalls et al. 2003</xref>; <xref ref-type="bibr" rid="mst109-B79">Puverel et al. 2005</xref>), the compositional analyses have shown that it is enriched in aspartic acid and, in lesser extent, in glutamic acid and glycine. This feature, together with the presence of saccharidic moieties, makes the OM unusually acidic. Moreover, its interaction with calcium carbonate was observed experimentally in the scleractinians <italic>Balanophyllia europaea</italic> (<xref ref-type="bibr" rid="mst109-B36">Goffredo et al. 2011</xref>), <italic>Acropora digitifera</italic>, <italic>Lophelia pertusa</italic>, <italic>Montipora caliculata</italic> (<xref ref-type="bibr" rid="mst109-B26">Falini et al. 2013</xref>) as well as by OM produced by coral cells of <italic>Stylophora pistillata</italic> (<xref ref-type="bibr" rid="mst109-B39">Helman et al. 2008</xref>), suggesting an important role of the OM in coral calcification.</p><p>Although several OM proteins have been extensively studied in other calcifying metazoans, such as molluscs (<xref ref-type="bibr" rid="mst109-B65">Marin et al. 2012</xref>) and sea urchins (<xref ref-type="bibr" rid="mst109-B96">Veis 2011</xref>), their characterization is still in its infancy for corals. Only recently an approach combining proteomics with genomics was applied unveiling a set of partial and complete sequences of proteins in the skeleton of the hexacoral <italic>S. </italic><italic>pistillata</italic> (<xref ref-type="bibr" rid="mst109-B24">Drake et al. 2013</xref>). Prior to this study, only the full sequence of two SOMPs, galaxin from the scleractinian <italic>Galaxea fascicularis</italic> (<xref ref-type="bibr" rid="mst109-B30">Fukuda et al. 2003</xref>), and scleritin from the octocoral <italic>Corallium rubrum</italic> (<xref ref-type="bibr" rid="mst109-B22">Debreuil et al. 2012</xref>) were published and a number of other partial sequences from octocorals (<xref ref-type="bibr" rid="mst109-B80">Rahman and Isa 2005</xref>; <xref ref-type="bibr" rid="mst109-B81">Rahman et al. 2005</xref>; <xref ref-type="bibr" rid="mst109-B82">Rahman, Isa, Takemura, et al. 2006</xref>; <xref ref-type="bibr" rid="mst109-B83">Rahman, Isa, Uehara 2006</xref>) and scleractinians (<xref ref-type="bibr" rid="mst109-B79">Puverel et al. 2005</xref>).</p><p>One prerequisite for understanding the process of coral calcification is a comprehensive survey of the skeletal matrix proteins. In this article, we address this requirement, by describing 36 extracellular proteins that constitute the SOMP repertoire, or “skeletome,” of the scleractinian coral <italic>Acropora millepora</italic>. This is the first large-scale survey of the proteins present in the skeleton of a member of the acroporid family and the second in cnidarians. These proteins give new insights on the molecular tools required for controlling the deposition of the calcium carbonate and provide a platform for investigating the impact of environmental factors on calcification at the molecular level. In addition, they open novel perspectives on the mechanisms of evolution of SOMPs within the Cnidaria and, more broadly, within other calcifying metazoans.</p></sec><sec><title>Results and Discussion</title><sec><title>Analysis of the Matrix on Gel</title><p>Coral skeleton was treated with bleach twice to remove contaminating tissue, symbionts, and bacteria, that can be entrapped in the highly porous skeleton (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online), the second treatment being conducted after the skeleton had been reduced to fine powder (granulometry < 200 µm). The residual powder was then extracted with 10% acetic acid, yielding acid-soluble matrix (ASM) and acid-insoluble matrix (AIM) fractions, assumed to be contaminant-free and closely associated with the aragonite skeleton. This extraction process yielded approximately 0.034 ± 0.01% of ASM and 0.23 ± 0.03% (wt/wt) of AIM relative to the skeleton weight. 1D-gel electrophoresis of the ASM extract (<xref ref-type="fig" rid="mst109-F1">fig. 1</xref><italic>A</italic>) showed three main discrete bands at around 120, 90, and 64 kDa embedded in a diffuse background of more dispersed “smearing” macromolecules, whereas the AIM was characterized by a smear lacking discrete bands. On the 2D gel (<xref ref-type="fig" rid="mst109-F1">fig. 1</xref><italic>B</italic>), the 90 and 64 kDa fractions, and, to a lesser extent, the 120 kDa fraction, in the ASM are characterized by large spots <italic>circa</italic> pI 3, suggesting that these macromolecules are very acidic.
<fig id="mst109-F1" position="float"><label>F<sc>ig</sc>. 1.</label><caption><p>Electrophoretic analysis of the ASM and AIM after AgNO<sub>3</sub> staining on (<italic>A</italic>) 12% poly-acrylamide SDS-polyacrylamide gel electrophoresis (PAGE) gel; (<italic>B</italic>) 4–10% precast poly-acrylamide gel using an IPG (3–10) strip in the first dimension, under denaturing conditions.</p></caption><graphic xlink:href="mst109f1p"></graphic></fig></p></sec><sec><title>Identification and Characterization of SOMPs</title><p>Raw data generated by LC-MS/MS on the ASM and AIM were analyzed with the software MASCOT to search against a pooled database consisting of transcripts and predicted proteins of <italic>A. </italic><italic>millepora</italic> and the genus <italic>Symbiodinium</italic>.</p><p>This procedure was initially made on the AIM and ASM prepared after only a single (whole skeleton) bleach treatment (“first batch”), but was then repeated on OM fractions (“second batch”) after the second bleaching step (i.e., on skeleton reduced to fine powder) outlined earlier. This enabled to analyze the effect of extended bleaching on removal of contaminants (<xref ref-type="fig" rid="mst109-F2">fig. 2</xref>). Although the proteomic analyses of the “first batch” revealed intracellular coral proteins and proteins of symbiont origin (<xref ref-type="bibr" rid="mst109-B85">Ramos-Silva et al. 2013</xref>), the second batch was free of such contaminants, enabling the detection of 43 unique <italic>A. </italic><italic>millepora</italic> proteins likely to constitute the SOMP repertoire, that is, proteins that are strongly associated to the skeleton. Of these 43 proteins, 36 assignments could be made with high confidence (i.e., with more than one unique peptide matching the sequence, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online), whereas seven sequences each with only a single peptide match were dropped from the list (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S2</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online) despite their properties being generally consistent with those of the high-confidence data set. <xref ref-type="fig" rid="mst109-F3">Figure 3</xref> provides a schematic overview of the domain structure and general properties of the <italic>Acropora</italic> SOMPs that were classified based on the in silico sequence analyses. From the 36 SOMPs identified, 22 proteins were common to both ASM and AIM (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). Similar ASM and AIM protein repertoires have also been observed in the case of mollusc shell matrices (<xref ref-type="bibr" rid="mst109-B60">Marie et al. 2009</xref>). On the other hand, 12 SOMPs were exclusively associated with the insoluble organic fraction of the skeleton, and only 2 with the soluble matrix (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). Possible causes for this apparent bias (<xref ref-type="fig" rid="mst109-F2">fig. 2</xref>) between matrices include the over/under representation of certain peptides that is inherent to the proteomic approach, and the fact that some proteins from the acid-soluble fraction may bind acid-insoluble components.
<fig id="mst109-F2" position="float"><label>F<sc>ig</sc>. 2.</label><caption><p>Comparison of the proteins identified by proteomics on the ASM and AIM in two different conditions, batch 1 and batch 2. Batch 1 consisted of treating the skeletal fragments with sodium hypochlorite once, whereas batch 2 consisted of batch 1 followed by a subsequent similar treatment on the skeletal-sieved powder (<200 µm). Extracts from batch 1 showed evidence of contamination with the identification of specific intracellular proteins from <italic>Acropora millepora</italic> (tubulins, histones, ATP-synthase) and proteins from zooxanthellae: 2 contaminants of 23 identifications in the ASM and 28 contaminants of 38 identifications in the AIM. In contrast, no contaminants were identified in batch 2, indicating that a second bleach treatment on powder is effective in removing potential sources of contamination and is required for obtaining exclusively SOMPs. The 22 SOMPs identified in batch 1 were also present in batch 2.</p></caption><graphic xlink:href="mst109f2p"></graphic></fig><fig id="mst109-F3" position="float"><label>F<sc>ig</sc>. 3.</label><caption><p>List of SOMPs identified by proteomics in the acid soluble and insoluble matrices extracted from <italic>Acropora millepora</italic> skeleton. Proteins were named according to the characterization of their primary structure.</p></caption><graphic xlink:href="mst109f3p"></graphic></fig></p><sec><title>Acidic Proteins</title><p>In both ASM and AIM, the most abundant proteins (high emPAI values; <xref ref-type="bibr" rid="mst109-B43">Ishihama et al. 2005</xref>; <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online) were acidic (<xref ref-type="fig" rid="mst109-F1">fig. 1</xref><italic>B</italic>), that is, with pI < 4.5 (<xref ref-type="bibr" rid="mst109-B63">Marin and Luquet 2007</xref>) and rich in negatively charged residues (Asp, Glu). Asp and Glu-rich proteins are supposed to interact directly with calcium carbonate crystals promoting crystal nucleation, determining the growth axes and inhibiting the crystal growth (<xref ref-type="bibr" rid="mst109-B99">Wheeler et al. 1981</xref>; <xref ref-type="bibr" rid="mst109-B1">Addadi et al. 1987</xref>). Because of their polyanionic character, Asp and Glu-rich proteins usually have high-capacity, but low-affinity, calcium-binding properties (<xref ref-type="bibr" rid="mst109-B66">Maurer et al. 1996</xref>). Among the <italic>A. millepora</italic> SOMPs, six acidic proteins were identified (<xref ref-type="fig" rid="mst109-F3">fig. 3</xref>), including two similar (48.8% identity; <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S2</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online) proteins each containing approximately 20% of Asp residues and named as SAARP1 (B3EWY6) and 2 (B3EWY8) (secreted acidic Asp-rich protein). These proteins are likely to belong to a family of highly acidic proteins conserved across scleractinians. Indeed two Asp-rich proteins, named coral acid-rich proteins (CARPs), were recently identified in <italic>S. </italic><italic>pistillata</italic> skeleton (<xref ref-type="bibr" rid="mst109-B24">Drake et al. 2013</xref>). Similarly to CARPs, SAARPs contain two Asp-rich regions intercalated by two nonacidic regions (<xref ref-type="fig" rid="mst109-F3">fig. 3</xref>). In particular, CARP 4 (M1SN56) exhibits the highest identity percentage with the two SAARPs, 60.6% and 45.2%, for SAARP 1 and 2, respectively. However, the predicted protein sequences of CARP4 and CARP5 (M1RYM2) are shorter than SAARPs and lack a putative peptide signal required for targeting these proteins to the secretory pathway. Interestingly, the four acidic proteins also show significant identity, in the nonacidic regions, with the protein fragment P27 (M1TA76) identified in the skeleton of <italic>S. pistillata</italic> (<xref ref-type="bibr" rid="mst109-B24">Drake et al. 2013</xref>) (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S3</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online); however, this similarity is not understood from a functional viewpoint.</p><p>The two SAARPs together with a third protein, acidic SOMP (B3EWY7), corresponded to the identifications with the highest emPAI scores (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). The acidic SOMP has lower Asp content (9.9%) and its sequence does not contain two Asp-rich regions as in SAARPs (<xref ref-type="fig" rid="mst109-F3">fig. 3</xref>). Still, it shows significant similarities in the nonacidic region with both SAARPs (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S4</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online).</p><p>The three other skeletal proteins included the secreted acidic proteins Amil-SAP1 (B3EWZ0, B3EWZ1), Amil-SAP2 (B3EWZ4) (19% and 21.6% of Asp and Glu, respectively), and one Glu-rich protein (B7W112). Amil-SAP1 and Amil-SAP2 are the counterparts of Adi-SAP1 (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S5</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online) and Adi-SAP2, respectively, which have been hypothesized to have roles in calcification along with seven other proteins, Adi-SAP3 to -SAP9, in <italic>Acropora digitifera</italic>, on the basis of their high Asp plus Glu content (<xref ref-type="bibr" rid="mst109-B87">Shinzato et al. 2011</xref>). Of these acidic proteins, only SAP1 and two exhibit a predicted signal peptide, and are confirmed here to be true SOMPs. BLASTP searches with the six acidic SOMPs retrieved significant matches (<italic>E</italic> value < 10<sup>−</sup><sup>4</sup>) with proteins in the UniprotKB database. Interestingly, among the best matches for Amil-SAP1 and the SAARPs were shell matrix proteins (aspeins) from <italic>Pinctada fucata</italic>, <italic>P. </italic><italic>maxima</italic><italic>,</italic> and <italic>Isognomon perna</italic>. However, in these cases, similarity scores might be largely misleading due to the relatively low complexity of the sequences, dominated by acidic residues; note also that none of these SOMPs had significant similarity to annotated domains from the InterPro database. Moreover, these data confirm the direct involvement of acidic proteins in skeletal formation in <italic>A. </italic><italic>millepora</italic>.</p></sec><sec><title>Unique Uncharacterized Proteins</title><p>High-throughput approaches such as proteomics and transcriptomics have often revealed completely novel proteins that lack conserved domains or significant database matches that would allow to classify them into families and to hypothesize about their functions (<xref ref-type="bibr" rid="mst109-B84">Ramos-Silva et al. 2012</xref>). Galaxin exemplifies the case of such “orphans” because its function remains unknown (<xref ref-type="bibr" rid="mst109-B86">Reyes-Bermudez et al. 2009</xref>). In <italic>A. millepora</italic> skeleton, we identified two distinct galaxins along with further eight uncharacterized “orphan” SOMPs (<xref ref-type="fig" rid="mst109-F3">fig. 3</xref>) referred here as USOMPs. These proteins do not constitute a single group and, with the exception of three proteins, exhibit no significant similarities. Two of these exceptions are galaxin proteins, and the third is provided by USOMP-4 (B8UU74), with a region near the C-terminus that has similarity to three of the acidic proteins discussed earlier (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S4</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). The presence of multiple galaxin-related genes has previously been reported (<xref ref-type="bibr" rid="mst109-B86">Reyes-Bermudez et al. 2009</xref>; <xref ref-type="bibr" rid="mst109-B87">Shinzato et al. 2011</xref>); one of the galaxins identified here corresponds to GenBank ADI50283.1, a previously known gene from <italic>A. millepora</italic> (<xref ref-type="bibr" rid="mst109-B86">Reyes-Bermudez et al. 2009</xref>). The second sequence (galaxin-2, B8UU51) is orthologous with galaxin 2 from <italic>A. digitifera</italic> (<xref ref-type="bibr" rid="mst109-B87">Shinzato et al. 2011</xref>). The two <italic>A. millepora</italic> galaxins have 31.5% identity overall, the di-Cys repeat region being most similar. Note that the di-Cys pattern might indicate a role in the assembly and support of the macromolecular framework (<xref ref-type="bibr" rid="mst109-B30">Fukuda et al. 2003</xref>). Indeed a multiple alignment with five galaxins and similar sequences from <italic>Nematostella vectensis</italic> (GIs: 156374951 and 156377965) and <italic>Hydra magnipapillata</italic> (GI: 221103149) shows the conservation of at least nine double Cys motifs (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S6</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). Similarly in <italic>S. pistillata</italic> skeleton, three proteins were identified, P12 (M1GFJ4), P16 (M1SN43), and P22 (M1T243) (<xref ref-type="bibr" rid="mst109-B24">Drake et al. 2013</xref>), which can fit in our description of uncharacterized proteins. From these, P16 and P22 are completely unique, whereas P12 shows similarities with USOMP8 (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S6</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online).</p></sec><sec><title>Extracellular Matrix-Like Proteins</title><p>Other SOMPs, although in some cases lacking clear overall matches, contain conserved regions or domains. The largest group comprises those with one or more domains usually associated with extracellular matrix (ECM) or cell-adhesion proteins from other metazoans. Amongst the 15 ECM-related proteins identified in the skeletal OM of <italic>A. millepora</italic> most contained multiple domains (<xref ref-type="fig" rid="mst109-F3">fig. 3</xref>). Several of these ECM-related SOMPs resembled human cell-adhesion proteins such as mucin-4 (mucin-like SOMP, B3EWY9) and hemicentin-1 (coadhesin, B3EWZ3) (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online), which are heavily glycosylated and rich in cysteine residues, thereby forming disulfide bridges involved in modulating their adhesive properties. SOMPs that exhibit mucin-like or co-adhesin signatures have previously been reported in the mineralizing matrices of molluscs (<xref ref-type="bibr" rid="mst109-B62">Marin et al. 2000</xref>; <xref ref-type="bibr" rid="mst109-B91">Suzuki et al. 2009</xref>; <xref ref-type="bibr" rid="mst109-B46">Joubert et al. 2010</xref>), such as thrombospondin type 1 repeats, von Willebrand factor type A and epidermal growth factor-like domains, which are typical of ECM proteins involved in cell–cell and cell–substrate adhesion and in the binding of other macromolecules (<xref ref-type="bibr" rid="mst109-B25">Engel 1991</xref>; <xref ref-type="bibr" rid="mst109-B73">Mosher and Adams 2012</xref>). Moreover, many ECM domains present in <italic>Acropora’s</italic> skeletal proteome have been detected in previous high-throughput proteomic studies on the OM of molluscs, sea urchin, chicken eggshell, and coral (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S5</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online) but only the polycystic kidney disease 1 (PKD1)-related protein (B8UU59) contains domains (lipoxygenase, egg-jelly receptor, and PKD/chitinase) not previously found in proteomic analyses of other skeletal organic matrices. On the other hand some of the data sets used in these comparisons consist of broader lists of proteins, including some of intracellular origin that may be derived from cell debris and are most likely not involved in biomineralization (<xref ref-type="bibr" rid="mst109-B57">Mann et al. 2010</xref>; <xref ref-type="bibr" rid="mst109-B102">Zhang et al. 2012</xref>). Thus, comparisons were interpreted carefully and taking into account other evidences such as the expression in skeleton secreting-tissues (<xref ref-type="bibr" rid="mst109-B44">Jonchère et al. 2010</xref>; <xref ref-type="bibr" rid="mst109-B58">Marie et al. 2012</xref>; <xref ref-type="bibr" rid="mst109-B102">Zhang et al. 2012</xref>), the direct interaction with CaCO<sub>3</sub> or the detection in the calcifying mediums (<xref ref-type="bibr" rid="mst109-B40">Hincke et al. 2000</xref>) (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S5</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online).</p><p>From the 15 ECM-related SOMPs, 8 have homology domains with proteins from the <italic>S. pistillata</italic> skeletal proteome (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary tables S5</ext-link> and <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">S6</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online): mucin-like, coadhesin, ectin (B3EWZ8), epidermal growth factor (EGF) and laminin G domain-containing protein (B8UU78), Meprin, A-5 protein, and receptor protein-tyrosine phosphatase Mu (MAM) and LDL-receptor domain-containing protein 1 (B3EWZ5) and 2 (B3EWZ6), zona pellucida domain-containing protein (G8HTB6), and protocaherin-like (B8V7Q1). Still, apart from protocadherin, we could not confirm candidate orthologous for these SOMPs in <italic>S. pistillata</italic> because most sequences correspond to fragments. Four ECM-related SOMPs were exclusively detected in the AIM, two of them having homologs usually found in the central nervous system in metazoans (<xref ref-type="bibr" rid="mst109-B6">Bieber et al. 1989</xref>; <xref ref-type="bibr" rid="mst109-B28">Frank and Kemler 2002</xref>)—protocadherin-like and neuroglian-like protein (B8VIW9), also the EGF and laminin G domain-containing protein and finally collagen (B8V7R6). The latter is homologous to the human alpha-2 type I collagen (P08123), which is found in bone (<xref ref-type="bibr" rid="mst109-B34">Glimcher 1959</xref>; <xref ref-type="bibr" rid="mst109-B97">Veis and Sabsay 1987</xref>). Fibrils of collagen are generally in close contact with the mineral phase and remain as an insoluble component of the organic fraction throughout the extraction process. Although collagen has been detected in the spicules of gorgonian (<xref ref-type="bibr" rid="mst109-B37">Goldberg 1974</xref>; <xref ref-type="bibr" rid="mst109-B47">Kingsley et al. 1990</xref>) and scleractinian corals (<xref ref-type="bibr" rid="mst109-B24">Drake et al. 2013</xref>), this is the first report of the occurrence of collagen type I in the OM of a coral skeleton.</p></sec><sec><title>Enzymes</title><p>Four of the SOMPs identified correspond to three types of enzymes (<xref ref-type="fig" rid="mst109-F3">fig. 3</xref>). The first enzyme corresponds to a 148 amino acid (aa) fragment (B8V7P3) of an alpha-type carbonic anhydrase (CA), a zinc metalloenzyme that catalyzes the reversible hydration of carbon dioxide. CA activity has been detected in the skeletal OM of both zooxanthellate (<italic>Acropora hebes</italic>; <xref ref-type="bibr" rid="mst109-B42">Isa and Yamazato 1984</xref>) and azooxanthellate (<italic>Tubastrea aurea</italic>; <xref ref-type="bibr" rid="mst109-B93">Tambutté et al. 2006</xref>) scleractinians.</p><p>In the case of the CA identified here, its best BLAST hit in UniprotKB is the α-CA from <italic>S. </italic><italic>pistillata</italic>, STPCA-2 (C0IX24) (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). This enzyme was previously shown to be highly active compared with other CAs and was localized in the oral endoderm and aboral tissue (<xref ref-type="bibr" rid="mst109-B5">Bertucci et al. 2011</xref>). Despite its high similarity with the cytosolic human carbonic anhydrase II, STPCA-2 has unusual features such as a putative signal peptide and an insert of 30 amino acids between positions 212 and 243. Moreover, the same protein was also identified in the skeleton of <italic>S. pistillata</italic> by proteomics (<xref ref-type="bibr" rid="mst109-B24">Drake et al. 2013</xref>). These results, together with the data presented here for <italic>Acropora</italic>, directly link one specific CA to skeleton formation. Similarly, to a mechanism proposed for some molluscs (<xref ref-type="bibr" rid="mst109-B71">Miyamoto et al. 1996</xref>), the CA found here may display its enzymatic function in the extracellular calcifying medium, and be subsequently occluded in the growing skeleton.</p><p>The second SOMP (B3EWZ9), hephaestin-like, is a secreted member of the copper oxidase family with 1,114 aa length, containing a copper-binding site and a cupredoxin domain. Hephaestin proteins function as copper-dependent ferroxidases, mainly involved in iron and copper metabolism at membranes (<xref ref-type="bibr" rid="mst109-B98">Vulpe et al. 1999</xref>; <xref ref-type="bibr" rid="mst109-B52">Lang et al. 2012</xref>). Although to date there is no experimental evidence of activity of this type in mineralizing matrices, it is reasonable to propose that the hephaestin-like protein catalyzes the oxidation of iron (from Fe<sup>2+</sup> to Fe<sup>3+</sup>) in the compartment where aragonite precipitation occurs. It was previously suggested that corals may entrap iron in the skeleton as a detoxification mechanism when high concentrations of the metal are present in the reef (<xref ref-type="bibr" rid="mst109-B9">Brown et al. 1991</xref>). Alternatively, the incorporation of iron in the skeleton of <italic>A. millepora</italic> may be a normal process, regulated by hephaestin. Interestingly, cupredoxin domains have also been identified in the shell of the mollusc <italic>Crassostrea gigas</italic> (in the L-ascorbate oxidase, K1PLZ9; Laccase-15, K1QQA2 and Laccase-18, K1PMS4) and in the tooth and spicules of the echinoderm <italic>S. purpuratus</italic> (in a protein similar to hephaestin, H3JMP5) (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S5</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). Finally, the third enzymatic function assigned is observed in two SOMPs (B8V7S0 and B8VIV4), exclusive to the AIM. It corresponds to proteases containing peptidase S1/S6, chymotrypsin/Hap and C1r/C1s, Uegf, Bone morphogenetic protein 1 (CUB) domains.</p><p>Even though they are present at relatively lower abundance (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online), the enzymes detected here may play crucial roles in the supply of bicarbonate ions at the site of calcification (CA), in the regulation of iron and copper metabolism (hephaestin), and in the assembly/cleavage of the OM (proteases).</p></sec><sec><title>Toxin-Like</title><p>One SOMP (B7W114) corresponds to a secreted protein that has high similarity (50%) with the N-terminus of a toxin (of 1,052 aa long) from the cephalopod <italic>Sepia esculenta</italic> (SE-cephalotoxin) (B2DCR8; BLASTP <italic>E</italic> value = 8<sup>−</sup><sup>43</sup>), whereas a number of toxins have been characterized from cubozoans (box jellyfish) (<xref ref-type="bibr" rid="mst109-B8">Brinkman et al. 2012</xref>) and anthozoans (sea anemones) (<xref ref-type="bibr" rid="mst109-B29">Frazão et al. 2012</xref>). However, very little is known about toxins in scleractinian corals, though studies have demonstrated antibacterial activity of the mucus (<xref ref-type="bibr" rid="mst109-B35">Gochfeld and Aeby 2008</xref>; <xref ref-type="bibr" rid="mst109-B33">Geffen et al. 2009</xref>).</p><p>The level of identity between B7W114 and the cephalotoxin (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S7</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online) is suggestive of conserved function. If the toxic character of the toxin-like SOMP is later confirmed, we may hypothesize that this protein acts in the coral skeleton in a similar way to the lysozyme in the chicken eggshell (<xref ref-type="bibr" rid="mst109-B40">Hincke et al. 2000</xref>), which due to its well-known anti-microbial properties was suggested to have a protective function in the eggshell in addition to its ability to interact with calcium carbonate.</p></sec><sec><title>Proteins with Transmembrane Domains</title><p>The occurrence of transmembrane (TM) proteins associated to calcium carbonate biominerals has been explained as contamination by soft tissues (<xref ref-type="bibr" rid="mst109-B102">Zhang et al. 2012</xref>). However, with the double-bleaching procedure used in the present case, this hypothesis is very unlikely and we suggest another explanation. Eleven SOMPs identified among the different groups described above contain putative TM domains. Ten of these SOMPs have a single TM domain located at their C-terminus (<xref ref-type="fig" rid="mst109-F3">fig. 3</xref>), the exception being the PKD1-related protein, which is predicted to contain 11 TM helices, 4 of which are part of the polycystin cation channel domain. SOMPs with TM domains represent about one half of the ECM group, one protein (hephaestin-like) in the enzyme group, one protein (uncharacterized SOMP-3) of the “orphan” group and two in the acidic group (SAARP 2 and SAP-1). We propose that the TM proteins detected here are cleaved in their extracellular region, becoming subsequently occluded in the forming biomineral. Three lines of evidence support this hypothesis. First, without exception, the 75 peptides detected by LC-MS/MS that correspond to the 11 TM domain-containing SOMPs belong to the extracellular region located at the N-terminal side of the proteins (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). Second two proteases were detected amongst the SOMPs having chymotrypsin/Hap domains that may constitute the required tool for cleaving the extracellular region of the TM domain-containing SOMPs. Third, the 11 TM-containing SOMPs possess predicted chymotrypsin cleavage sites in their extracellular regions (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). Specific cleavage sites for chymotrypsin and TM domains are illustrated in <xref ref-type="fig" rid="mst109-F4">figure 4</xref> for two SOMPs: SAARP2 and zona pellucida domain-containing protein. These lines of evidence are congruent with the recent data on the spicule matrix proteins of the sea urchin <italic>S. purpuratus</italic> (<xref ref-type="bibr" rid="mst109-B57">Mann et al. 2010</xref>), so the mechanism proposed here may apply more generally.
<fig id="mst109-F4" position="float"><label>F<sc>ig</sc>. 4.</label><caption><p>Primary structures of TM SAARP2 and zona pellucida domain-containing protein, including: putative peptide signals (underlined), codon stop (*), TM domains (□), peptides identified by MSMS (red) and chymotrypsin high specificity cleavage sites (residues [FYW] not before P highlighted in green) with more than 80% of cleavage probability.</p></caption><graphic xlink:href="mst109f4p"></graphic></fig></p><p>If this hypothesis is later confirmed by other approaches, this may call for a complete change of paradigm in the biomineralization field, whatever the biological model studied. Until now, the “molecular tools” controlling the mineralization process were all supposed to be secreted outside the calcifying epithelial cells to interact with the inorganic precursors of mineralization. The data presented here suggest that membrane-bound proteins may also contribute to the process via their extracellular domains, which are subsequently incorporated in the biomineral after being cleaved by peptidases. Furthermore, it suggests that there is a true connection between the epithelium and the mineral front, and that the mineral deposition is accomplished under the direct guidance of the cell surfaces, rather than remotely, as “classical” views tend to show, in particular in mollusc shell models (<xref ref-type="bibr" rid="mst109-B100">Wilbur and Saleuddin 1983</xref>).</p></sec><sec><title>SOMPs in Early Stages of Calcification Affected by High CO<sub>2</sub></title><p>The availability of transcriptome data derived from <italic>A. </italic><italic>millepora</italic> primary polyps (<xref ref-type="bibr" rid="mst109-B74">Moya et al. 2012</xref>) permitted investigation of the impact of elevated CO<sub>2</sub> on expression of the SOMPs identified here. Comparisons with the data sets submitted with the NCBI Gene Expression Omnibus database (GEO) under the accession number GSE33016 confirmed that genes corresponding to all of the SOMPs are expressed during the early stages of calcification and at least 26 of these were up regulated, whereas 4 were down regulated in polyps exposed to higher concentrations of CO<sub>2</sub> (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S4</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). SOMPs are therefore appropriate molecular markers for the investigation of the impacts of elevated CO<sub>2</sub> on <italic>Acropora</italic> calcification.</p></sec></sec><sec><title>Homology Comparison between <italic>Acropora</italic>, <italic>N. </italic><italic>vectensis</italic>, and <italic>H. </italic><italic>magnipapillata</italic></title><p>In an attempt to unravel the origins of the <italic>A. millepora</italic> “skeletome”, homologs of the 36 biomineralization proteins were searched amongst the predicted proteins (using BLASTP) and corresponding transcripts (BLASTN and TBLASTX) from the three cnidarians for which whole genome sequence data are presently available—<italic>A</italic><italic>. digitifera,</italic> a scleractinian coral which diverged from <italic>A. millepora</italic> in the Mio-Pliocene (<xref ref-type="bibr" rid="mst109-B95">Van Oppen et al. 2001</xref>), the sea anemone <italic>N. </italic><italic>vectensis,</italic> and <italic>H. magnipapillata</italic>. Sea anemones are classified as a distinct Order (Actinaria) from corals (Scleractinia) but in the same subclass (Zoantharia = Hexacorallia) within Class Anthozoa. Although sea anemones are considered to be “close,” but skeleton-less, relatives of corals, <italic>Hydra</italic> is a phylogenetically remote cnidarian belonging to Class Hydrozoa (<xref ref-type="bibr" rid="mst109-B14">Collins 2002</xref>; <xref ref-type="bibr" rid="mst109-B4">Ball et al. 2004</xref>; <xref ref-type="bibr" rid="mst109-B15">Collins et al. 2006</xref>). This approach enabled proteins that are involved in <italic>Acropora</italic> biomineralization but have ancient cnidarian origins (i.e., are present in <italic>Hydra</italic> as well as <italic>Acropora</italic>) to be resolved from those that may be anthozoan-specific but not restricted to calcifying anthozoans (i.e., present in <italic>Nematostella</italic> as well as <italic>Acropora</italic>) and those that are so far unique to stony corals. Clear orthologs of each of the 36 <italic>A. millepora</italic> SOMPs could be identified in <italic>A. digitifera</italic> (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary tables S1</ext-link> and <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">S3</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online), confirming that all the sequences assigned to <italic>A. millepora</italic> are of coral origin and are not derived from zooxanthellae.</p><p>BLAST comparisons against the predicted proteins of <italic>N. </italic><italic>vectensis</italic> and <italic>H. </italic><italic>magnipapillata</italic>, indicate that eight of the SOMPs do not have counterparts in the two noncalcifying cnidarians: five “orphan” proteins (USOMPs), SAP-1, SAP-2, and the SOMP similar to cephalotoxin (outer circle, <xref ref-type="fig" rid="mst109-F5">fig. 5</xref>). Indeed, SAPs were previously suggested to be restricted to members of the genus <italic>Acropora</italic> (<xref ref-type="bibr" rid="mst109-B87">Shinzato et al. 2011</xref>).
<fig id="mst109-F5" position="float"><label>F<sc>ig</sc>. 5.</label><caption><p>Resume of the results of similarity searches with BLAST and homology detection (by global alignment, domain architecture comparison and the NC method) using the 36 SOMPs (and corresponding transcripts) from <italic>Acropora millepora</italic> and the genomes of <italic>A. digitifera</italic>, <italic>Nematostella vectensis,</italic> and <italic>Hydra magnipapillata</italic>. Proteins in the outer circle (red) do not have similarities (and homologs) in the predicted proteins of <italic>Nematostella</italic> or <italic>Hydra</italic>. Proteins in the middle circle (green) have homologs in <italic>Acropora</italic> and <italic>Nematostella</italic>. Proteins from the inner circle (blue) have homologs in <italic>Acropora</italic>, <italic>Nematostella,</italic> and <italic>Hydra</italic>. SOMPs in the white region of the circle show considerable similarity with proteins from <italic>Nematostella</italic> and <italic>Hydra</italic> but their homology is not certain. The phylogenetic tree on the upright side represents the relationships previously purposed between Cnidaria (<xref ref-type="bibr" rid="mst109-B15">Collins et al. 2006</xref>); dcp, domain containing protein.</p></caption><graphic xlink:href="mst109f5p"></graphic></fig></p><p>To date, a BLAST search against the cnidarian sequence data on NCBI with these proteins only retrieved orthologous sequences in other <italic>Acropora</italic> species (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S3</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online) and low sequence similarities (<italic>E</italic> value < 10<sup>−</sup><sup>5</sup>) in <italic>Porites astreoides</italic> (USOMP-2), <italic>Montastrea faveolata</italic> (USOMP-2, USOMP-4), <italic>Aiptasia pallida</italic> (USOMP-1, USOMP-3), <italic>Anemonia viridis</italic> (USOMP-3), and <italic>Clytia hemisphaerica</italic> (similar to cephalotoxin). In particular, the apparent absence of homologs of the cephalotoxin-like sequence in other cnidarians is surprising given the similarity between the <italic>Acropora</italic> and cuttlefish (<italic>Sepia</italic>) proteins. Note that the SE-cephalotoxin (B2DCR8) identified in the salivary glands of the cuttlefish <italic>Sepia esculenta</italic> (Cephalopoda, Mollusca) does not exhibit any significant similarity to known proteins (<xref ref-type="bibr" rid="mst109-B94">Ueda et al. 2008</xref>). In turn, the striking similarity (50%) between the <italic>Acropora</italic> and <italic>Sepia</italic> sequences (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S6</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online) suggests that the ancestor of these proteins predates the divergence of molluscs and corals (i.e., 550–600 Ma or more) but could have been lost in most of the other eumetazoan lineages. Such a genome restructuring, that is, massive loss, has been recently documented via large-scale comparisons of distantly related genomes: for example, the gastropod <italic>Lottia</italic> and <italic>Nematostella</italic> share exclusively 89 gene families that are not retrieved in other phyla investigated (<xref ref-type="bibr" rid="mst109-B78">Ptitsyn and Moroz 2012</xref>).</p><p>Amongst the other SOMPs, we have distinguished similar (white circle area, <xref ref-type="fig" rid="mst109-F5">fig. 5</xref>) from homologous (green and blue circles, <xref ref-type="fig" rid="mst109-F5">fig. 5</xref>) proteins taking into account the statistical significance of BLAST searches, the percentage of identity (cutoff 30%) in the pairwise global alignment, the common domain architecture and the fact that many of the SOMPs are mosaic proteins, that is, proteins with multiple domains (<xref ref-type="bibr" rid="mst109-B7">Bork 1992</xref>; <xref ref-type="bibr" rid="mst109-B23">Doolittle 1992</xref>) (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S3</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). In particular for those proteins from the ECM-like group and enzymes, it is difficult to infer homology merely on the basis of BLAST searches (generally highly significant within a certain domain) together with identity percentages and alignment coverage (generally low). To overcome this difficulty, the Neighborhood Correlation (NC) method was applied to the 3 genomes. This method has been developed and used to accurately identify homologs in complex multidomain families (<xref ref-type="bibr" rid="mst109-B88">Song et al. 2008</xref>; <xref ref-type="bibr" rid="mst109-B45">Joseph and Durand 2009</xref>), enabling the prediction of similar proteins (due to domain insertion) from homologous proteins (due to common ancestor).</p><p>This combined analysis implied that at least three SOMPs have homologs in the anthozoan but not in <italic>H. magnipapillata</italic> (middle circle, <xref ref-type="fig" rid="mst109-F5">fig. 5</xref>): USOMP-7, neuroglian-like and zona pellucida domain-containing protein, whereas other 9 SOMPs, all from the ECM-like group, have homologs in the three genomes (inner circle, <xref ref-type="fig" rid="mst109-F5">fig. 5</xref>). Several of these conserved ECM-like SOMPs are also found in vertebrates, such as the protocadherin-like, collagen, and PKD1-related proteins (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). Whether these proteins function in similar ways in the skeletal matrix and in the ECM remains to be clarified. However, these results strongly corroborate the idea of a modern skeletal matrix derived from the recruitment of noncalcifying ECM-components.</p><p>Conversely, homologs for 16 of the SOMPS could not be confidently identified in the noncalcifying cnidarians due to specific factors: incompleteness of at least one of the sequences in study (putative carbonic-anhydrase, peptidase, Glu-rich, and USOMP-5), low identity percentages (cutoff 30%) (hephaestin-like, galaxins, SAARPs, acidic SOMP, and USOMP-8), different combination of domain architectures and neighborhood coefficients above the threshold (mucin-like, coadhesin, threonine-rich, and CUB domain-containing protein, peptidase) (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S3</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). In consequence, these SOMPs were considered as “similar to” and not confirmed as homologous or nonhomologous (white circle area, <xref ref-type="fig" rid="mst109-F5">fig. 5</xref>). Interestingly, homologs of hephaestin are present in both chordates and the symbiotic sea anemone <italic>Anemonia viridis</italic> whereas only low similarity matches were identified in <italic>N. vectensis</italic> and <italic>H. magnipapillata</italic> (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S8</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). Also the SOMP mucin-like has similar domain architecture in the C-terminus side (extracellular nidogen, adhesion associated domain in mucin-4 and other proteins, von Willebrand factor D, and EGF) to the human mucin-4 found in chordates; however, the same domain combination was not identified in the genomes of <italic>Nematostella</italic> and <italic>Hydra</italic> (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S3</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online).</p></sec></sec><sec sec-type="conclusions"><title>Conclusions</title><p>The work described here represents the first comprehensive survey of the skeletal molecular toolkit of the scleractinian coral <italic>A. </italic><italic>millepora</italic>. In total, 36 proteins were identified from the acid soluble and insoluble skeletal organic matrices. The proteins of these two fractions overlapped significantly, only 2 proteins being exclusively associated with the soluble fraction and 12 with the insoluble fraction. It is unclear how complete this survey is; trypsin-resistant skeletal proteins, in particular those that do not possess suitable trypsin cleavage sites, may not have been detected. It is also possible that the calicoblastic cells secrete proteins that guide the calcification process but are not occluded in the skeleton and would therefore not be detectable by the approach used here.</p><p>One tenet of our analyses is that the classical view of a coral skeletal matrix composed primarily or exclusively of acidic proteins (<xref ref-type="bibr" rid="mst109-B70">Mitterer 1978</xref>; <xref ref-type="bibr" rid="mst109-B16">Constantz and Weiner 1988</xref>) is eclipsed by the idea of a more complex matrix consisting of a diverse range of proteins with a larger spectrum of different potential functions. Whilst acidic proteins remain quantitatively key players in calcification (as aptly illustrated by the 2D gel), other SOMPs, some present in lower amount, are also likely to fulfill important roles in the deposition of the skeleton. The second tenet of our analysis is that the SOMPs are not exclusively secreted. Some SOMPs are membrane-bound proteins, the extracellular domains of which are subsequently cleaved in the mineralizing space and occluded in the growing biomineral.</p><p>What kind of information on the macroevolution of calcifying matrices can be inferred from this analysis? The evolutionary picture that emerges from our analysis is multifaceted. Whilst all of the SOMPs identified in <italic>A. millepora</italic> have homologs encoded in the genome of <italic>Acropora digitifera</italic>, surprising few of these are actually unique to the scleractinians, that is, present in the two acroporids but absent from noncalcifying cnidarians (<italic>N. vectensis</italic> and <italic>H. magnipapillata</italic>). These coral-restricted proteins may be “novelties” in the calcification repertoire. The timing of this innovation might have occurred deep in the Paleozoic when scleractinian corals emerged (∼450 Ma) (<xref ref-type="bibr" rid="mst109-B89">Stolarski et al. 2011</xref>). In contrast, a considerable proportion of the ECM-like SOMPs identified here have homologous proteins in <italic>N. vectensis</italic> and <italic>H. magnipapillata</italic>, suggesting that their origins predate the divergence of the classes Anthozoa and Hydrozoa (<xref ref-type="bibr" rid="mst109-B4">Ball et al. 2004</xref>), which occurred in the Cambrian or earlier. Each of these proteins, and a number of others subsequently lost from <italic>N. vectensis</italic> and <italic>H. magnipapillata</italic> (typified by the proteins similar to cephalotoxin, hephaestin-like, and mucin-like), was presumably co-opted to function in calcification when this trait arose in the scleractinian lineage. Broadening the comparison with include other OM proteomes from metazoans revealed that most of the domains represented in the <italic>A. millepora</italic> skeletal proteome also occur in other OM, pleading for the concept of a pool of “shared domains” potentially involved in biomineralization. However, only few of the proteins containing these common domains are predicted to be homologous, suggesting that they have been independently recruited to roles in calcification at different times across the various taxa with this trait. Beyond macroevolutionary considerations, the data set presented here constitutes an important tool for understanding the molecular bases of calcification in stony corals, and for quantifying the impact of environmental changes (such as OA), on this process.</p></sec><sec sec-type="materials|methods"><title>Materials and Methods</title><sec><title>Skeletal Collection and SEM Observations</title><p><italic>Acropora millepora</italic> colonies were collected at the Great Barrier Reef in Australia (Pioneer Bay, Orpheus Island) in November 2010, prior to the annual spawning event. Mother colonies that died after spawning were used to collect the skeletal material; animal tissue, symbionts, and other micro-organisms were removed by immersion in NaOCl (5%, vol/vol) for 72 h. The skeletal material was then rinsed with purified water, dried and mechanically fragmented. The skeleton microstructure was observed with a tabletop Scanning Electron Microscope (SEM, Hitachi TM-1000) under an acceleration voltage of 15 keV. Mirror polished transverse and longitudinal sections were observed after etching in EDTA (1 % wt/vol, 3 min), rinsed with water and dried. The skeletal fragments were then split in two batches. The first one was not submitted to further treatment before mineral dissolution for matrix extraction, whereas the second batch was treated further (discussed later).</p></sec><sec><title>OM Extraction</title><p>Skeletal fragments of the second batch were reduced to powder (Fritsch Pulverisette 14), which was subsequently sieved (particle size below 200 µm). The powder was bleached in NaOCl solution (10 times dilution, 0.26% active chlorine) for 5 h and washed with milli-Q water several times until no trace of NaOCl was left. This treatment allows removing organic exogenous or endogenous contaminants that can be entrapped in the highly porous skeleton (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary fig. S1</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online), while keeping intact the most tightly bound skeletal matrix components (<xref ref-type="bibr" rid="mst109-B31">Gaffey and Bronnimann 1993</xref>). The extraction was performed according to a published procedure (<xref ref-type="bibr" rid="mst109-B64">Marin et al. 2001</xref>). In brief, the dried powder put in suspension in cold water was decalcified overnight in acetic acid (10% vol/vol) at 4 °C with an electronic burette (Titronic Universal, Schott, Mainz, Germany). The solution was centrifuged: the organic pellet (AIM) was rinsed several times with Milli-Q water and freeze-dried. The supernatant (ASM) was treated on an ultrafiltration cell (Amicon 400 mL, 10 kDa cutoff membrane) for volume reduction, then once concentrated, dialyzed several days against water at 4 °C, and freeze-dried. The extraction was performed in duplicate (2 × 30 g of skeletal powder under the same NaOCl treatment) to check the reproducibility of the results.</p></sec><sec><title>ASM/AIM Analysis on 1D and 2D Gel Electrophoresis</title><p>The skeletal matrix—both ASM and AIM fractions—was analyzed on 1D electrophoresis and stained with silver nitrate (<xref ref-type="bibr" rid="mst109-B72">Morrissey 1981</xref>). The ASM was directly denatured with Laemmli buffer (<xref ref-type="bibr" rid="mst109-B51">Laemmli 1970</xref>) according to standard conditions while the AIM was only partly solubilized by Laemmli buffer. The solubilized fraction is defined as the Laemmli-soluble/acetic acid insoluble fraction (LS/AIM). Electrophoresis was performed on discontinuous 12% acrylamide mini-gels at 100 V for 15 min and 150 V for 1 h. For the 2D electrophoresis, immobilized pH gradient (IPG) strips (ReadyStrip, BioRad) were loaded with 180 μg of ASM dissolved in 180 μL of rehydratation buffer (6 M urea, 2 M thiourea, 4% [wt/vol] Chaps, 20 mM dithiothreitol, 0.1% ampholytes, 0.001% bromophenol blue) and rehydrated overnight at 50 V (25 °C) in a PROTEAN IEF cell (BioRad). Focusing was carried out at 250 V for 15 min, followed by 4,000 V for 2 h and 4,000 V until 10,000 Vh. Subsequently the IPG strips were equilibrated by transfer for 10 min into 1 mL of equilibration buffer I (6 M urea, 2% sodium dodecyl sulphate [SDS], 375 mM Tris/HCl pH 8.8, 20% glycerol) with 2% (wt/vol) dithiothreitol, followed by 10 min in equilibration buffer II, with 2.5% (wt/vol) iodoacetamide instead. Strips were rinsed in 25 mM Tris, 192 mM glycine and 0.1% SDS (TGS) and placed on the top of precast NuPAGE(R) BisTris Novex SDS-polyacrylamide gels (4–10%) (Invitrogen, Carlsbad, CA) with an overlay solution of 0.5% agarose/TGS (wt/vol). Electrophoresis was performed at 200 V for 35 min. Both 1D and 2D gels were stained with silver nitrate (<xref ref-type="bibr" rid="mst109-B72">Morrissey 1981</xref>).</p></sec><sec><title>Proteomic Analysis</title><p>The AIM and ASM were prepared for proteomic analysis according to a routine procedure including reduction, alkylation, trypsin digestion, drying, and solubilization in TFA (<xref ref-type="bibr" rid="mst109-B60">Marie et al. 2009</xref>, <xref ref-type="bibr" rid="mst109-B61">2010</xref>). MS analyses were performed in duplicate on a LTQ Velos (ThermoScientific, France) instrument in the positive ion mode. The ion source was equipped with a picoTip emitter as nanospray needle (FS360-75-30-CE-5-C10.5, NewObjective, USA) operating at 1.5 kV. The acquisition was done with the Excalibur 2.1 software (ThermoScientific). Typically, two scan events were used: at first, <italic>m</italic>/<italic>z</italic> 400–1,600 survey scan MS with enhanced resolution; second, data-dependent scans MS/MS on the 20 most intense ions from the previous event. The spectra were recorded using dynamic exclusion of previously analyzed ions for 0.6 min. The MS/MS normalized collision energy was set to 35 eV.</p><p>LC was performed on an Ultimate 3000 nano-LC system (Dionex, Voisins Le Bretonneux, France). Chromatographic separation of peptides was obtained with a C<sub>18</sub> PepMap micro-precolumn (5 μm; 0.3 mm × 5 mm) for a 3 min desalting and a C<sub>18</sub> PepMap nano-column (3 μm; 100 Å; 75 μm × 150 mm) with a gradient elution at a flow rate of 300 nL/min. Eluent A was a mixture of 95% H<sub>2</sub>O, 5% CH<sub>3</sub>CN, and 0.1% formic acid (vol/vol). Eluent B was a mixture of 20% H<sub>2</sub>O, 80% CH<sub>3</sub>CN, and 0.1% formic acid (vol/vol). The gradient program was from 0% B to 50% B over 120 min and 100% B for 10 min.</p></sec><sec><title>In Silico Analysis of the SOMPs</title><p>The data obtained with the LTQ-velos system were used for protein identification with the MASCOT search engine (version 2.1, Matrix Science, London, UK). MS/MS raw data were searched with carbamidomethylation as fixed modification, and methionine oxidation, asparagine, and glutamine de-amidation as variable modifications. The tolerance of the precursor and fragment masses was set to 0.4 Da. Proteins identified with at least two distinct peptides were considered valid assignments. The corresponding peptide sequences were validated by manually inspection of the MS/MS spectra. The subject of MASCOT searches was a pooled protein database comprising the 6-frame translated nucleotide sequences from different publicly available sources in April 2012: <italic>A. </italic><italic>millepora</italic> sequences were downloaded from NCBI including 101,380 plus 15,389 sequences from the nucleotide and EST databases, respectively. EST (7,964) and nucleotide (5,112) data from the genus <italic>Symbiodinium</italic> were also included together with the assembled EST of two <italic>Symbiodinium</italic> strains (Mf104b – clade B [76,284 EST] and KB8 – clade A [72,152 EST]) (<xref ref-type="bibr" rid="mst109-B105">Bayer et al. 2012</xref>) from <ext-link ext-link-type="uri" xlink:href="http://medinalab.org/zoox">http://medinalab.org/zoox</ext-link> (last accessed October 3, 2012). The proteins identified through MASCOT were used in similarity searches against the UniprotKB/Swissprot database. Subsequently, protein sequences were analyzed for the presence of signal peptides with Signal IP 4.0 (<ext-link ext-link-type="uri" xlink:href="http://www.cbs.dtu.dk/services/SignalP/">http://www.cbs.dtu.dk/services/SignalP/</ext-link>, last accessed February 15, 2013), TM domains with TMHMM v. 2.0 (<ext-link ext-link-type="uri" xlink:href="http://www.cbs.dtu.dk/services/TMHMM/">http://www.cbs.dtu.dk/services/TMHMM/</ext-link>, last accessed February 15, 2013) and further characterized for homologous domains, and regions using the InterproScan platform (<ext-link ext-link-type="uri" xlink:href="http://www.ebi.ac.uk/Tools/pfa/iprscan/">http://www.ebi.ac.uk/Tools/pfa/iprscan/</ext-link>, last accessed February 15, 2013). In addition, the proteins with TM domains were also analyzed for potential cleavage sites cleaved by proteases with PeptideCutter (<ext-link ext-link-type="uri" xlink:href="http://web.expasy.org/peptide_cutter/">http://web.expasy.org/peptide_cutter/</ext-link>, last accessed December 11, 2012).</p></sec><sec><title>Homology Analysis and Protein Comparisons at the Domain Level</title><p>Homology analysis were performed using the 36 <italic>A. millepora</italic> sequences involved in biomineralization (transcripts and proteins) against the predicted coding genes of <italic>Acropora digitifera</italic> (<ext-link ext-link-type="uri" xlink:href="http://marinegenomics.oist.jp/genomes/gallery">http://marinegenomics.oist.jp/genomes/gallery</ext-link>, last accessed November 2, 2011), <italic>N. </italic><italic>vectensis</italic> (<ext-link ext-link-type="uri" xlink:href="http://www.uniprot.org/">http://www.uniprot.org/</ext-link>, last accessed February 14, 2013), and <italic>H. </italic><italic>magnipapillata</italic> (<ext-link ext-link-type="ftp" xlink:href="ftp://ftp.jgi-psf.org/pub/JGI_data/Hydra_magnipapillata/">ftp://ftp.jgi-psf.org/pub/JGI_data/Hydra_magnipapillata/</ext-link>, last accessed February 14, 2013). First, searches with a local BLAST (version 2.2.25+) (<xref ref-type="bibr" rid="mst109-B3">Altschul et al. 1990</xref>) were performed using the transcripts (and protein sequences) of the 36 SOMPs from <italic>A. millepora</italic> against: 1) the coding genes of <italic>A. </italic><italic>digitifera</italic> (TBLASTN), with default parameters; 2) the coding genes from <italic>N. vectensis</italic> and <italic>H. magnipapillata</italic> (TBLASTX), with default parameters; 3) the predicted proteins of the three cnidarian genomes (BLASTP), with and without SEG (i.e., low complexity filter on query sequence) (<xref ref-type="bibr" rid="mst109-B101">Wootton and Federhen 1993</xref>). The best hit for each SOMP (<italic>E</italic> value threshold <10<sup>−</sup><sup>4</sup>) was selected and the sequences globally aligned using Needleman–Wunsch algorithm for pairwise aligment (cutoff: 30% identity, expect for mosaic proteins) (<ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">supplementary table S3</ext-link>, <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary Material</ext-link> online). The best matches were also manually compared for their domain architecture. Second, the NC method (<ext-link ext-link-type="uri" xlink:href="http://www.neighborhoodcorrelation.org/">http://www.neighborhoodcorrelation.org/</ext-link>, last accessed December 12, 2012) (<xref ref-type="bibr" rid="mst109-B88">Song et al. 2008</xref>) was implemented for homology identification between the three genomes (<italic>A. digitifera</italic> vs. <italic>N. vectensis</italic> and <italic>A. digitifera</italic> vs. <italic>H. magnipapillata</italic>). Multidomain proteins with high confidence NC coefficients (>0.8) were considered strictly homologs (<xref ref-type="bibr" rid="mst109-B45">Joseph and Durand 2009</xref>). Orthologs of SOMPs in <italic>A. digitifera</italic> genome were selected and subsequently analyzed for the presence of the homologs in <italic>N. vectensis</italic> and <italic>H. magnipapillata</italic>. The SOMPs and corresponding best matches in the three genomes that were not complete sequences but showed significant blast scores (<italic>E</italic> value < 10<sup>−</sup><sup>4</sup>), were not assessed for their homology and just considered similar.</p><p>High-throughput proteomic data sets obtained from OMs occluded in CaCO<sub>3</sub> structures were collected from the literature and their primary sequences analyzed with InterProScan: <italic>Strongylocentrotus purpuratus</italic> (tooth: <xref ref-type="bibr" rid="mst109-B55">Mann et al. 2008a</xref>; spicules: <xref ref-type="bibr" rid="mst109-B57">Mann et al. 2010</xref>; test and spine: <xref ref-type="bibr" rid="mst109-B56">Mann et al. 2008b</xref>), <italic>Gallus gallus</italic> (eggshell: <xref ref-type="bibr" rid="mst109-B54">Mann et al. 2006</xref>; <xref ref-type="bibr" rid="mst109-B68">Miksík et al. 2010</xref>), <italic>Lottia gigantea</italic> (shell: <xref ref-type="bibr" rid="mst109-B58">Marie et al. 2013</xref>), <italic>Crassostrea gigas</italic> (shell: <xref ref-type="bibr" rid="mst109-B102">Zhang et al. 2012</xref>), and <italic>Pinctada</italic> (shell: <xref ref-type="bibr" rid="mst109-B58">Marie et al. 2012</xref>). The complete set of signatures obtained in these proteomes (InterPro entries - domains and repeats) was compared with those present in the SOMPs.</p></sec></sec><sec sec-type="supplementary-material"><title>Supplementary Material</title><p><ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">Supplementary tables S1–S6</ext-link> and <ext-link ext-link-type="uri" xlink:href="http://mbe.oxfordjournals.org/lookup/suppl/doi:10.1093/molbev/mst109/-/DC1">figures S1–S8</ext-link> are available at <italic>Molecular Biology and Evolution</italic> online (<ext-link ext-link-type="uri" xlink:href="http://www.mbe.oxfordjournals.org/">http://www.mbe.oxfordjournals.org/</ext-link>).</p><supplementary-material id="PMC_1" content-type="local-data"><caption><title>Supplementary Data</title></caption><media mimetype="text" mime-subtype="html" xlink:href="supp_30_9_2099__index.html"></media><media xlink:role="associated-file" mimetype="application" mime-subtype="pdf" xlink:href="supp_mst109_Supporting_File_1.pdf"></media><media xlink:role="associated-file" mimetype="application" mime-subtype="pdf" xlink:href="supp_mst109_Supporting_File_2.pdf"></media><media xlink:role="associated-file" mimetype="application" mime-subtype="vnd.ms-excel" xlink:href="supp_mst109_Table_S3.xlsx"></media><media xlink:role="associated-file" mimetype="application" mime-subtype="vnd.ms-excel" xlink:href="supp_mst109_Table_S1.xlsx"></media><media xlink:role="associated-file" mimetype="application" mime-subtype="vnd.ms-excel" xlink:href="supp_mst109_Table_S2.xlsx"></media></supplementary-material></sec></body><back><ack><title>Acknowledgments</title><p>P.R.S. carried out the protein extractions, proteomic, and sequence analysis, and drafted the manuscript. J.K. participated in the design of the study and data analysis. L.H. collected and prepared the coral samples prior to extraction and contributed with scientific input for the manuscript. N.G. performed new coral decalcifications to check the reproducibility of the extraction procedure and contributed with technical input. I.Z.C. performed the mass spectrometry experiments. B.M. and D.J.M. contributed to the interpretation of the data and writing of the manuscript. F.M. participated in the design of the study, data analysis and drafting of the manuscript. This work was supported by the <funding-source>EU FP7 Marie Curie Initial Training Network BIOMINTEC</funding-source> (<award-id>PITN-GA-2008-215507</award-id>, <ext-link ext-link-type="uri" xlink:href="www.biomintec.de">www.biomintec.de</ext-link>, coordinator H.C. Schröder) and by the <funding-source>EU FP7 Knowledge Based Bio-Economy project BioPreDyn</funding-source> grant <award-id>289434</award-id> (<ext-link ext-link-type="uri" xlink:href="www.biopredyn.eu">www.biopredyn.eu</ext-link>) to P.R.S. and by the ANR project (ACCRO-EARTH, ref. <award-id>BLAN06-2_159971</award-id>, <funding-source>Gilles Ramstein, LSCE</funding-source>; during the period 2007–2011) and the <funding-source>COST project</funding-source> TD0903 (“Biomineralix”, <ext-link ext-link-type="uri" xlink:href="www.biomineralix.eu">www.biomineralix.eu</ext-link>, 2009–2013) to P.R.-S., B.M., N.G., and F.M. The authors thank Dr J.M. 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