Characterization of the TRBP domain required for Dicer interaction and function in RNA interference
Identifieur interne : 007C45 ( Main/Curation ); précédent : 007C44; suivant : 007C46Characterization of the TRBP domain required for Dicer interaction and function in RNA interference
Auteurs : Sylvanne M. Daniels [Canada] ; Carlos E. Melendez-Pe A [Canada] ; Robert J. Scarborough [Canada] ; Aïcha Daher [Canada] ; Helen S. Christensen [Australie] ; Mohamed El Far [Canada] ; Damian Fj Purcell [Australie] ; Sébastien Lainé [Canada, France] ; Anne Gatignol [Canada]Source :
- BMC Molecular Biology [ 1471-2199 ] ; 2009.
Abstract
Dicer, Ago2 and TRBP are the minimum components of the human RNA-induced silencing complex (RISC). While Dicer and Ago2 are RNases, TRBP is the double-stranded RNA binding protein (dsRBP) that loads small interfering RNA into the RISC. TRBP binds directly to Dicer through its C-terminal domain.
We show that the TRBP binding site in Dicer is a 165 amino acid (aa) region located between the ATPase and the helicase domains. The binding site in TRBP is a 69 aa domain, called C4, located at the C-terminal end of TRBP. The TRBP1 and TRBP2 isoforms, but not TRBPs lacking the C4 site (TRBPsΔC4), co-immunoprecipitated with Dicer. The C4 domain is therefore necessary to bind Dicer, irrespective of the presence of RNA. Immunofluorescence shows that while full-length TRBPs colocalize with Dicer, TRBPsΔC4 do not.
The mapping of the interaction sites between TRBP and Dicer show unique domains that are required for their binding. Since TRBPsΔC4 do not interact or colocalize with Dicer, we suggest that TRBP and Dicer, both dsRBPs, do not interact through bound dsRNA. TRBPs, but not TRBPsΔC4, rescue RNAi activity in RNAi-compromised cells, indicating that the binding of Dicer to TRBP is critical for RNAi function.
Url:
DOI: 10.1186/1471-2199-10-38
PubMed: 19422693
PubMed Central: 2685382
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PMC:2685382Le document en format XML
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<author><name sortKey="Daher, Aicha" sort="Daher, Aicha" uniqKey="Daher A" first="Aïcha" last="Daher">Aïcha Daher</name>
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<author><name sortKey="El Far, Mohamed" sort="El Far, Mohamed" uniqKey="El Far M" first="Mohamed" last="El Far">Mohamed El Far</name>
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<author><name sortKey="Purcell, Damian Fj" sort="Purcell, Damian Fj" uniqKey="Purcell D" first="Damian Fj" last="Purcell">Damian Fj Purcell</name>
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<author><name sortKey="Laine, Sebastien" sort="Laine, Sebastien" uniqKey="Laine S" first="Sébastien" last="Lainé">Sébastien Lainé</name>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Dicer, Ago2 and TRBP are the minimum components of the human RNA-induced silencing complex (RISC). While Dicer and Ago2 are RNases, TRBP is the double-stranded RNA binding protein (dsRBP) that loads small interfering RNA into the RISC. TRBP binds directly to Dicer through its C-terminal domain.</p>
</sec>
<sec><title>Results</title>
<p>We show that the TRBP binding site in Dicer is a 165 amino acid (aa) region located between the ATPase and the helicase domains. The binding site in TRBP is a 69 aa domain, called C4, located at the C-terminal end of TRBP. The TRBP1 and TRBP2 isoforms, but not TRBPs lacking the C4 site (TRBPsΔC4), co-immunoprecipitated with Dicer. The C4 domain is therefore necessary to bind Dicer, irrespective of the presence of RNA. Immunofluorescence shows that while full-length TRBPs colocalize with Dicer, TRBPsΔC4 do not. <italic>tarbp2</italic>
<sup>-/- </sup>
cells, which do not express TRBP, do not support RNA interference (RNAi) mediated by short hairpin or micro RNAs against EGFP. Both TRBPs, but not TRBPsΔC4, were able to rescue RNAi function. In human cells with low RNAi activity, addition of TRBP1 or 2, but not TRBPsΔC4, rescued RNAi function.</p>
</sec>
<sec><title>Conclusion</title>
<p>The mapping of the interaction sites between TRBP and Dicer show unique domains that are required for their binding. Since TRBPsΔC4 do not interact or colocalize with Dicer, we suggest that TRBP and Dicer, both dsRBPs, do not interact through bound dsRNA. TRBPs, but not TRBPsΔC4, rescue RNAi activity in RNAi-compromised cells, indicating that the binding of Dicer to TRBP is critical for RNAi function.</p>
</sec>
</div>
</front>
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