AustralieFrV1, Istex, Corpus, bibRecord, 001845

Cytotoxic and genotoxic effects of cylindrospermopsin in mice treated by gavage or intraperitoneal injection

Identifieur interne : 001845 ( Istex/Corpus ); précédent : 001844; suivant : 001846

Cytotoxic and genotoxic effects of cylindrospermopsin in mice treated by gavage or intraperitoneal injection

Auteurs : Emmanuelle Bazin ; Sylvie Huet ; Gérard Jarry ; Ludovic Le Hégarat ; John S. Munday ; Andrew R. Humpage ; Valérie Fessard

Source :

Environmental Toxicology [ 1520-4081 ] ; 2012-05.

RBID : ISTEX:813041481D3CB98D0D28713AD6149B193E29F18A

English descriptors

Abstract

Cylindrospermopsin (CYN), a cyanobacterial hepatotoxin mainly produced by Cylindrospermopsis raciborskii, has been involved in human intoxications and livestock deaths. The widespread occurrence of CYN in the water supplies lead us to investigate its genotoxicity to assess potential chronic effects. This study reports evaluation of CYN‐induced in vivo DNA damage in mice using alkaline comet assay (ACA) and micronucleus assay (MNA) concomittantly. ACA measures DNA breakage from single and double strand breaks as well as alkali labile sites. Conversely, MNA detects chromosome damage events such as chromosomal breakage and numeric alterations. Male Swiss mice were treated with CYN concentrations of 50, 100, and 200 μg/kg by a single intraperitoneal (ip) injection or with 1, 2, and 4 mg/kg by gavage. Methyl methane sulfonate (MMS) was used as positive control at 80 mg/kg. Twenty‐four hours after treatment, samples of liver, blood, bone marrow, kidney, intestine, and colon were taken to perform ACA, the bone marrow and the colon were also used for MNA. Parameters used to quantify DNA damage were % Tail DNA for ACA and both micronucleated immature erythrocytes and epithelial colon cells for MNA. DNA breaks and chromosome damage were significantly increased by MMS in all the organs evaluated. Significant DNA damage was detected within the colon by ACA after ip injection of 100 and 200 μg/kg CYN (P < 0.01). DNA damage was also detected in colon samples after 4 mg/kg oral administration of CYN and in bone marrow after 1 and 2 mg/kg of orally administered CYN. Histological examination showed foci of cell death within the liver and the kidney from mice that received the two highest doses of CYN by either route of administration. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012.

Url:

https://api.istex.fr/document/813041481D3CB98D0D28713AD6149B193E29F18A/fulltext/pdf

DOI: 10.1002/tox.20640

Links to Exploration step

ISTEX:813041481D3CB98D0D28713AD6149B193E29F18A

Le document en format XML

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<term>Alkaline comet assay</term>
<term>Anabaena lapponica</term>
<term>Apoptosis</term>
<term>Apoptotic</term>
<term>Apoptotic cells</term>
<term>Assay</term>
<term>Australian water quality center</term>
<term>Bone marrow</term>
<term>Bone marrow cells</term>
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<term>Colon</term>
<term>Colon cells</term>
<term>Colonic cells</term>
<term>Comet</term>
<term>Comet assay</term>
<term>Corticomedullary junction</term>
<term>Cyanobacterial</term>
<term>Cyanobacterial toxin</term>
<term>Cylindrospermopsin</term>
<term>Cylindrospermopsis</term>
<term>Cylindrospermopsis raciborskii</term>
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<term>Cytotoxicity</term>
<term>Dead cells</term>
<term>Dos</term>
<term>Environ</term>
<term>Environmental toxicology</term>
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<term>Erythrocyte</term>
<term>Falconer</term>
<term>First report</term>
<term>Gastrointestinal tract</term>
<term>Gavage</term>
<term>Genotoxic</term>
<term>Genotoxic effects</term>
<term>Genotoxicity</term>
<term>Hbss medium</term>
<term>Histological</term>
<term>Histological examination</term>
<term>Histological lesions</term>
<term>Humpage</term>
<term>Intraperitoneal</term>
<term>Intraperitoneal injection route</term>
<term>Liquid stools</term>
<term>Lyngbya wollei</term>
<term>Median</term>
<term>Metabolic activation</term>
<term>Methyl methane sulfonate</term>
<term>Micronucleated</term>
<term>Micronucleus</term>
<term>Micronucleus assay</term>
<term>Micronucleus test</term>
<term>Mitotic cells</term>
<term>Mouse</term>
<term>Mouse tissues</term>
<term>Multiple foci</term>
<term>Mutat</term>
<term>Negative control</term>
<term>Normochromatic erythrocytes</term>
<term>Norris</term>
<term>Oral administration</term>
<term>Oral dose</term>
<term>Oral dosing</term>
<term>Oral exposure</term>
<term>Oral toxicity</term>
<term>Physiological saline</term>
<term>Polychromatic erythrocytes</term>
<term>Positive control</term>
<term>Preliminary evidence</term>
<term>Previous studies</term>
<term>Raciborskii</term>
<term>Raphidiopsis curvata</term>
<term>Rodent genotoxicity assays</term>
<term>Room temperature</term>
<term>Seawright</term>
<term>Sodium pentobarbital</term>
<term>Statistical analysis</term>
<term>Target organs</term>
<term>Toxicity</term>
<term>Toxicol</term>
<term>Toxicology</term>
<term>Toxin</term>
<term>Treatment group</term>
<term>Tubular epithelial cells</term>
<term>Water supplies</term>
<term>Wiley periodicals</term>
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<term>Alkaline comet assay</term>
<term>Anabaena lapponica</term>
<term>Apoptosis</term>
<term>Apoptotic</term>
<term>Apoptotic cells</term>
<term>Assay</term>
<term>Australian water quality center</term>
<term>Bone marrow</term>
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<term>Cell death</term>
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<term>Colon cells</term>
<term>Colonic cells</term>
<term>Comet</term>
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<term>Cylindrospermopsin</term>
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<term>First report</term>
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<term>Gavage</term>
<term>Genotoxic</term>
<term>Genotoxic effects</term>
<term>Genotoxicity</term>
<term>Hbss medium</term>
<term>Histological</term>
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<term>Humpage</term>
<term>Intraperitoneal</term>
<term>Intraperitoneal injection route</term>
<term>Liquid stools</term>
<term>Lyngbya wollei</term>
<term>Median</term>
<term>Metabolic activation</term>
<term>Methyl methane sulfonate</term>
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<term>Micronucleus assay</term>
<term>Micronucleus test</term>
<term>Mitotic cells</term>
<term>Mouse</term>
<term>Mouse tissues</term>
<term>Multiple foci</term>
<term>Mutat</term>
<term>Negative control</term>
<term>Normochromatic erythrocytes</term>
<term>Norris</term>
<term>Oral administration</term>
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<term>Oral dosing</term>
<term>Oral exposure</term>
<term>Oral toxicity</term>
<term>Physiological saline</term>
<term>Polychromatic erythrocytes</term>
<term>Positive control</term>
<term>Preliminary evidence</term>
<term>Previous studies</term>
<term>Raciborskii</term>
<term>Raphidiopsis curvata</term>
<term>Rodent genotoxicity assays</term>
<term>Room temperature</term>
<term>Seawright</term>
<term>Sodium pentobarbital</term>
<term>Statistical analysis</term>
<term>Target organs</term>
<term>Toxicity</term>
<term>Toxicol</term>
<term>Toxicology</term>
<term>Toxin</term>
<term>Treatment group</term>
<term>Tubular epithelial cells</term>
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<front><div type="abstract" xml:lang="en">Cylindrospermopsin (CYN), a cyanobacterial hepatotoxin mainly produced by Cylindrospermopsis raciborskii, has been involved in human intoxications and livestock deaths. The widespread occurrence of CYN in the water supplies lead us to investigate its genotoxicity to assess potential chronic effects. This study reports evaluation of CYN‐induced in vivo DNA damage in mice using alkaline comet assay (ACA) and micronucleus assay (MNA) concomittantly. ACA measures DNA breakage from single and double strand breaks as well as alkali labile sites. Conversely, MNA detects chromosome damage events such as chromosomal breakage and numeric alterations. Male Swiss mice were treated with CYN concentrations of 50, 100, and 200 μg/kg by a single intraperitoneal (ip) injection or with 1, 2, and 4 mg/kg by gavage. Methyl methane sulfonate (MMS) was used as positive control at 80 mg/kg. Twenty‐four hours after treatment, samples of liver, blood, bone marrow, kidney, intestine, and colon were taken to perform ACA, the bone marrow and the colon were also used for MNA. Parameters used to quantify DNA damage were % Tail DNA for ACA and both micronucleated immature erythrocytes and epithelial colon cells for MNA. DNA breaks and chromosome damage were significantly increased by MMS in all the organs evaluated. Significant DNA damage was detected within the colon by ACA after ip injection of 100 and 200 μg/kg CYN (P < 0.01). DNA damage was also detected in colon samples after 4 mg/kg oral administration of CYN and in bone marrow after 1 and 2 mg/kg of orally administered CYN. Histological examination showed foci of cell death within the liver and the kidney from mice that received the two highest doses of CYN by either route of administration. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012.</div>
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<json:item><name>Gérard Jarry</name>
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<json:item><name>Ludovic Le Hégarat</name>
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<titleInfo type="abbreviated" lang="en"><title>Cytotoxic and Genotoxic Effects of Cyn in Mice</title>
</titleInfo>
<titleInfo type="alternative" contentType="CDATA" lang="en"><title>Cytotoxic and genotoxic effects of cylindrospermopsin in mice treated by gavage or intraperitoneal injection</title>
</titleInfo>
<name type="personal"><namePart type="given">Emmanuelle</namePart>
<namePart type="family">Bazin</namePart>
<affiliation>Agence Française de Sécurité Sanitaire des Aliments, Unité de Toxicologie Génétique des Contaminants Alimentaires, BP 90 203, 35 302 Fougères Cedex, France</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Sylvie</namePart>
<namePart type="family">Huet</namePart>
<affiliation>Agence Française de Sécurité Sanitaire des Aliments, Unité de Toxicologie Génétique des Contaminants Alimentaires, BP 90 203, 35 302 Fougères Cedex, France</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Gérard</namePart>
<namePart type="family">Jarry</namePart>
<affiliation>Agence Française de Sécurité Sanitaire des Aliments, Unité de Toxicologie Génétique des Contaminants Alimentaires, BP 90 203, 35 302 Fougères Cedex, France</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Ludovic Le</namePart>
<namePart type="family">Hégarat</namePart>
<affiliation>Agence Française de Sécurité Sanitaire des Aliments, Unité de Toxicologie Génétique des Contaminants Alimentaires, BP 90 203, 35 302 Fougères Cedex, France</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">John S.</namePart>
<namePart type="family">Munday</namePart>
<affiliation>Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Andrew R.</namePart>
<namePart type="family">Humpage</namePart>
<affiliation>Cooperative Research Center for Water Quality and Treatment, GPO Box 1751 Adelaide, 5001 Australia</affiliation>
<affiliation>Australian Water Quality Center, GPO Box 1751 Adelaide, 5001 Australia</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
</name>
<name type="personal"><namePart type="given">Valérie</namePart>
<namePart type="family">Fessard</namePart>
<affiliation>Agence Française de Sécurité Sanitaire des Aliments, Unité de Toxicologie Génétique des Contaminants Alimentaires, BP 90 203, 35 302 Fougères Cedex, France</affiliation>
<affiliation>E-mail: valerie.fessard@anses.fr</affiliation>
<affiliation>Correspondence address: Agence Française de Sécurité Sanitaire des Aliments, Unité de Toxicologie Génétique des Contaminants Alimentaires, BP 90 203, 35 302 Fougères Cedex, France</affiliation>
<role><roleTerm type="text">author</roleTerm>
</role>
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<genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre>
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<place><placeTerm type="text">Hoboken</placeTerm>
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<dateIssued encoding="w3cdtf">2012-05</dateIssued>
<dateCaptured encoding="w3cdtf">2010-02-13</dateCaptured>
<dateValid encoding="w3cdtf">2010-06-30</dateValid>
<copyrightDate encoding="w3cdtf">2012</copyrightDate>
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<language><languageTerm type="code" authority="rfc3066">en</languageTerm>
<languageTerm type="code" authority="iso639-2b">eng</languageTerm>
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<abstract lang="en">Cylindrospermopsin (CYN), a cyanobacterial hepatotoxin mainly produced by Cylindrospermopsis raciborskii, has been involved in human intoxications and livestock deaths. The widespread occurrence of CYN in the water supplies lead us to investigate its genotoxicity to assess potential chronic effects. This study reports evaluation of CYN‐induced in vivo DNA damage in mice using alkaline comet assay (ACA) and micronucleus assay (MNA) concomittantly. ACA measures DNA breakage from single and double strand breaks as well as alkali labile sites. Conversely, MNA detects chromosome damage events such as chromosomal breakage and numeric alterations. Male Swiss mice were treated with CYN concentrations of 50, 100, and 200 μg/kg by a single intraperitoneal (ip) injection or with 1, 2, and 4 mg/kg by gavage. Methyl methane sulfonate (MMS) was used as positive control at 80 mg/kg. Twenty‐four hours after treatment, samples of liver, blood, bone marrow, kidney, intestine, and colon were taken to perform ACA, the bone marrow and the colon were also used for MNA. Parameters used to quantify DNA damage were % Tail DNA for ACA and both micronucleated immature erythrocytes and epithelial colon cells for MNA. DNA breaks and chromosome damage were significantly increased by MMS in all the organs evaluated. Significant DNA damage was detected within the colon by ACA after ip injection of 100 and 200 μg/kg CYN (P < 0.01). DNA damage was also detected in colon samples after 4 mg/kg oral administration of CYN and in bone marrow after 1 and 2 mg/kg of orally administered CYN. Histological examination showed foci of cell death within the liver and the kidney from mice that received the two highest doses of CYN by either route of administration. © 2010 Wiley Periodicals, Inc. Environ Toxicol, 2012.</abstract>
<subject lang="en"><genre>keywords</genre>
<topic>cylindrospermopsin</topic>
<topic>genotoxicity</topic>
<topic>in vivo alkaline comet assay</topic>
<topic>in vivo micronucleus assay</topic>
</subject>
<relatedItem type="host"><titleInfo><title>Environmental Toxicology</title>
</titleInfo>
<titleInfo type="abbreviated"><title>Environ. Toxicol.</title>
</titleInfo>
<genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre>
<identifier type="ISSN">1520-4081</identifier>
<identifier type="eISSN">1522-7278</identifier>
<identifier type="DOI">10.1002/(ISSN)1522-7278</identifier>
<identifier type="PublisherID">TOX</identifier>
<part><date>2012</date>
<detail type="volume"><caption>vol.</caption>
<number>27</number>
</detail>
<detail type="issue"><caption>no.</caption>
<number>5</number>
</detail>
<extent unit="pages"><start>277</start>
<end>284</end>
<total>8</total>
</extent>
</part>
</relatedItem>
<relatedItem type="preceding"><titleInfo><title>Environmental Toxicology and Water Quality</title>
</titleInfo>
<identifier type="ISSN">1053-4725</identifier>
<identifier type="ISSN">1098-2256</identifier>
<part><date point="end">1998</date>
<detail type="volume"><caption>last vol.</caption>
<number>13</number>
</detail>
<detail type="issue"><caption>last no.</caption>
<number>4</number>
</detail>
</part>
</relatedItem>
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<identifier type="ark">ark:/67375/WNG-BP149MRZ-7</identifier>
<identifier type="DOI">10.1002/tox.20640</identifier>
<identifier type="ArticleID">TOX20640</identifier>
<accessCondition type="use and reproduction" contentType="copyright">Copyright © 2010 Wiley Periodicals, Inc.</accessCondition>
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Cytotoxic and genotoxic effects of cylindrospermopsin in mice treated by gavage or intraperitoneal injection

Cytotoxic and genotoxic effects of cylindrospermopsin in mice treated by gavage or intraperitoneal injection

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