Pyruvate oxidase and oxaloacetate decarboxylase enzyme electrodes
Identifieur interne : 000283 ( France/Analysis ); précédent : 000282; suivant : 000284Pyruvate oxidase and oxaloacetate decarboxylase enzyme electrodes
Auteurs : Sophie Peguin [France] ; Pierre R. Coulet [France] ; Gilbert Bardeletti [France]Source :
- Analytica Chimica Acta [ 0003-2670 ] ; 1988.
Abstract
Pyruvate, glutamate pyruvate transaminase (GPT), or glutamate oxaloacetate transaminase (GOT) were determined with a specific enzyme electrode using pyruvate oxidase alone or mixed with oxaloacetate decarboxylase, immobilized on a preactivated membrane. Conditions for immobilization, assays and membrane storage were optimized. For the detection of pyruvate in foodstuffs or biological samples, the detection limit was 1–10−5 M with a linear calibration range of 2–10−5 1–0.1 M. The correlation coefficient with a commercial kit was 0.996. With a two-electrode-based analyzer using pyruvate oxidase immobilized on one electrode and oxaloacetate decarboxylase and pyruvate oxidase co-immobilized and set on the second electrode, GOT and GPT activities were simultaneously determined in < 4 min under the same conditions at optimized substrate concentrations. A linear range of 6–30 000 U 1−1 in the sample, a detection limit of 3 U 1−1, with RSD values of 4.1% and 4.5% and correlation coefficients with the commercial kit of 0.997 and 0.996 for GPT and GOT, respectively, were obtained.
Url:
DOI: 10.1016/S0003-2670(00)81882-6
Affiliations:
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Conditions for immobilization, assays and membrane storage were optimized. For the detection of pyruvate in foodstuffs or biological samples, the detection limit was 1–10−5 M with a linear calibration range of 2–10−5 1–0.1 M. The correlation coefficient with a commercial kit was 0.996. With a two-electrode-based analyzer using pyruvate oxidase immobilized on one electrode and oxaloacetate decarboxylase and pyruvate oxidase co-immobilized and set on the second electrode, GOT and GPT activities were simultaneously determined in < 4 min under the same conditions at optimized substrate concentrations. A linear range of 6–30 000 U 1−1 in the sample, a detection limit of 3 U 1−1, with RSD values of 4.1% and 4.5% and correlation coefficients with the commercial kit of 0.997 and 0.996 for GPT and GOT, respectively, were obtained.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li></region><settlement><li>Lyon</li></settlement><orgName><li>Université Claude Bernard Lyon 1</li></orgName></list><tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Peguin, Sophie" sort="Peguin, Sophie" uniqKey="Peguin S" first="Sophie" last="Peguin">Sophie Peguin</name></region><name sortKey="Bardeletti, Gilbert" sort="Bardeletti, Gilbert" uniqKey="Bardeletti G" first="Gilbert" last="Bardeletti">Gilbert Bardeletti</name><name sortKey="Coulet, Pierre R" sort="Coulet, Pierre R" uniqKey="Coulet P" first="Pierre R." last="Coulet">Pierre R. 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