Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures.
Identifieur interne : 000217 ( PubMed/Corpus ); précédent : 000216; suivant : 000218Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures.
Auteurs : S. Papanikolaou ; I. Chevalot ; M. Komaitis ; I. Marc ; G. AggelisSource :
- Applied microbiology and biotechnology [ 0175-7598 ] ; 2002.
English descriptors
- KwdEn :
- Air Movements, Ammonium Sulfate (metabolism), Animals, Bioreactors (microbiology), Culture Media, Fatty Acids (metabolism), Fermentation, Food Industry, Glycerol (metabolism), Hot Temperature, Hydrogen-Ion Concentration, Industrial Oils, Kinetics, Lipid Metabolism, Lipids (biosynthesis), Oils (metabolism), Stearic Acids (analysis), Stearic Acids (metabolism), Yarrowia (growth & development), Yarrowia (metabolism).
- MESH :
- chemical , analysis : Stearic Acids.
- chemical , biosynthesis : Lipids.
- chemical , metabolism : Ammonium Sulfate, Fatty Acids, Glycerol, Oils, Stearic Acids.
- growth & development : Yarrowia.
- metabolism : Yarrowia.
- microbiology : Bioreactors.
- Air Movements, Animals, Culture Media, Fermentation, Food Industry, Hot Temperature, Hydrogen-Ion Concentration, Industrial Oils, Kinetics, Lipid Metabolism.
Abstract
The growth of an oleaginous strain of Yarrowia lipolytica on an industrial fat composed of saturated free fatty acids (stearin) was studied. Lipid accumulation during primary anabolic growth was critically influenced by the medium pH and the incubation temperature. This process was independent of the nitrogen concentration in the culture medium, but was favored at a high carbon substrate level and at a low aeration rate. At pH 6 and a temperature of 28-33 degrees C, 9-12 g/l of dry biomass was produced, whereas significant quantities of lipids were accumulated inside the yeast cells (0.44-0.54 g of lipid per gram of biomass). The strain showed the tendency to degrade its storage lipids, although significant amounts of substrate fat, rich in stearic acid, remained unconsumed in the culture medium. Y. lipolytica presented a strong fatty acid specificity. The fatty acids C12:0, C14:0, and C16:0 were rapidly incorporated and mainly used for growth needs, while C18:0 was incorporated with reduced rates and was mainly accumulated as storage material. Reserve lipids, principally composed of triacylglycerols (55% w/w of total lipids) and free fatty acids (35% w/w), were rich in stearic acid (80% w/w), while negligible amounts of unsaturated fatty acids were detected. When industrial glycerol was used as co-substrate, together with stearin, unsaturated fatty acid concentration in the reserve lipid increased.
DOI: 10.1007/s00253-001-0897-0
PubMed: 11935181
Links to Exploration step
pubmed:11935181Le document en format XML
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<author><name sortKey="Chevalot, I" sort="Chevalot, I" uniqKey="Chevalot I" first="I" last="Chevalot">I. Chevalot</name>
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<author><name sortKey="Komaitis, M" sort="Komaitis, M" uniqKey="Komaitis M" first="M" last="Komaitis">M. Komaitis</name>
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<author><name sortKey="Marc, I" sort="Marc, I" uniqKey="Marc I" first="I" last="Marc">I. Marc</name>
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<author><name sortKey="Aggelis, G" sort="Aggelis, G" uniqKey="Aggelis G" first="G" last="Aggelis">G. Aggelis</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures.</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Air Movements</term>
<term>Ammonium Sulfate (metabolism)</term>
<term>Animals</term>
<term>Bioreactors (microbiology)</term>
<term>Culture Media</term>
<term>Fatty Acids (metabolism)</term>
<term>Fermentation</term>
<term>Food Industry</term>
<term>Glycerol (metabolism)</term>
<term>Hot Temperature</term>
<term>Hydrogen-Ion Concentration</term>
<term>Industrial Oils</term>
<term>Kinetics</term>
<term>Lipid Metabolism</term>
<term>Lipids (biosynthesis)</term>
<term>Oils (metabolism)</term>
<term>Stearic Acids (analysis)</term>
<term>Stearic Acids (metabolism)</term>
<term>Yarrowia (growth & development)</term>
<term>Yarrowia (metabolism)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Stearic Acids</term>
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<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Lipids</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ammonium Sulfate</term>
<term>Fatty Acids</term>
<term>Glycerol</term>
<term>Oils</term>
<term>Stearic Acids</term>
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<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Yarrowia</term>
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<term>Culture Media</term>
<term>Fermentation</term>
<term>Food Industry</term>
<term>Hot Temperature</term>
<term>Hydrogen-Ion Concentration</term>
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<front><div type="abstract" xml:lang="en">The growth of an oleaginous strain of Yarrowia lipolytica on an industrial fat composed of saturated free fatty acids (stearin) was studied. Lipid accumulation during primary anabolic growth was critically influenced by the medium pH and the incubation temperature. This process was independent of the nitrogen concentration in the culture medium, but was favored at a high carbon substrate level and at a low aeration rate. At pH 6 and a temperature of 28-33 degrees C, 9-12 g/l of dry biomass was produced, whereas significant quantities of lipids were accumulated inside the yeast cells (0.44-0.54 g of lipid per gram of biomass). The strain showed the tendency to degrade its storage lipids, although significant amounts of substrate fat, rich in stearic acid, remained unconsumed in the culture medium. Y. lipolytica presented a strong fatty acid specificity. The fatty acids C12:0, C14:0, and C16:0 were rapidly incorporated and mainly used for growth needs, while C18:0 was incorporated with reduced rates and was mainly accumulated as storage material. Reserve lipids, principally composed of triacylglycerols (55% w/w of total lipids) and free fatty acids (35% w/w), were rich in stearic acid (80% w/w), while negligible amounts of unsaturated fatty acids were detected. When industrial glycerol was used as co-substrate, together with stearin, unsaturated fatty acid concentration in the reserve lipid increased.</div>
</front>
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<Title>Applied microbiology and biotechnology</Title>
<ISOAbbreviation>Appl. Microbiol. Biotechnol.</ISOAbbreviation>
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<ArticleTitle>Single cell oil production by Yarrowia lipolytica growing on an industrial derivative of animal fat in batch cultures.</ArticleTitle>
<Pagination><MedlinePgn>308-12</MedlinePgn>
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<Abstract><AbstractText>The growth of an oleaginous strain of Yarrowia lipolytica on an industrial fat composed of saturated free fatty acids (stearin) was studied. Lipid accumulation during primary anabolic growth was critically influenced by the medium pH and the incubation temperature. This process was independent of the nitrogen concentration in the culture medium, but was favored at a high carbon substrate level and at a low aeration rate. At pH 6 and a temperature of 28-33 degrees C, 9-12 g/l of dry biomass was produced, whereas significant quantities of lipids were accumulated inside the yeast cells (0.44-0.54 g of lipid per gram of biomass). The strain showed the tendency to degrade its storage lipids, although significant amounts of substrate fat, rich in stearic acid, remained unconsumed in the culture medium. Y. lipolytica presented a strong fatty acid specificity. The fatty acids C12:0, C14:0, and C16:0 were rapidly incorporated and mainly used for growth needs, while C18:0 was incorporated with reduced rates and was mainly accumulated as storage material. Reserve lipids, principally composed of triacylglycerols (55% w/w of total lipids) and free fatty acids (35% w/w), were rich in stearic acid (80% w/w), while negligible amounts of unsaturated fatty acids were detected. When industrial glycerol was used as co-substrate, together with stearin, unsaturated fatty acid concentration in the reserve lipid increased.</AbstractText>
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<Author ValidYN="Y"><LastName>Komaitis</LastName>
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<Author ValidYN="Y"><LastName>Marc</LastName>
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<Author ValidYN="Y"><LastName>Aggelis</LastName>
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<Language>eng</Language>
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