LrgpV1, PascalFrancis, Corpus, bibRecord, 000676

Evaluation of microscopic techniques (epifluorescence microscopy, CLSM, TPE-LSM) as a basis for the quantitative image analysis of activated sludge

Identifieur interne : 000676 ( PascalFrancis/Corpus ); précédent : 000675; suivant : 000677

Evaluation of microscopic techniques (epifluorescence microscopy, CLSM, TPE-LSM) as a basis for the quantitative image analysis of activated sludge

Auteurs : C. Lopez ; M. N. Pons ; E. Morgenroth

Source :

Water research : (Oxford) [ 0043-1354 ] ; 2005.

RBID : Pascal:05-0102743

Descripteurs français

Pascal (Inist)
- Epuration eau usée, Epuration biologique, Boue activée, Floc, Analyse image, Analyse structurale, Agrégat, Analyse quantitative, Microscopie épifluorescence, Microscopie confocale, Microscope laser, Microscope balayage, Excitation 2 photons.

English descriptors

KwdEn :
- Activated sludge, Aggregate, Biological purification, Confocal microscopy, Epifluorescence microscopy, Flock, Image analysis, Laser microscope, Quantitative analysis, Scanning microscope, Structural analysis, Two photon excitation, Waste water purification.

Abstract

Microscopic techniques ranging from epifluorescence microscopy to confocal laser scanning microscopy (CLSM) and two photon excitation laser scanning microscopy (TPE-LSM) combined with fluorescent stains can help to evaluate complex microbial aggregates such as activated sludge flocs. To determine the application limits of these microscopic techniques, activated sludge samples from three different sources were evaluated after staining with a fluorescent viability indicator (Baclight Bacterial Viability Kit, Molecular Probes). Image analysis routines were developed to quantify overall amounts of red and green stained cells, location of stained cells within the flocs, and the spatial organization in clusters and filaments. It was found that the selection of the appropriate microscopic technique depends strongly on the type of microbial aggregates being analyzed. For flocs with high cell density, the use of TPE-LSM is preferred, since it provides a clearer image of the internal structure of the aggregate. Epifluorescence microscopy did not allow to reliably quantify red stained cells in dense aggregates. CLSM did not adequately image the internal filamentous structure and the location of stained cells within dense flocs. However, for typical activated sludge flocs epifluorescence and CLSM proved adequate.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0043-1354`
A02	`01`			`@0 WATRAG`
A03		`1`		`@0 Water res. : (Oxf.)`
A05				`@2 39`
A06				`@2 2-3`
A08	`01`	`1`	`ENG`	`@1 Evaluation of microscopic techniques (epifluorescence microscopy, CLSM, TPE-LSM) as a basis for the quantitative image analysis of activated sludge`
A11	`01`	`1`		`@1 LOPEZ (C.)`
A11	`02`	`1`		`@1 PONS (M. N.)`
A11	`03`	`1`		`@1 MORGENROTH (E.)`
A14	`01`			`@1 Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 3219 Newmark Civil Engineering Laboratory, 250, 205 North Mathews Avenue @2 Urbana, IL 61801 @3 USA @Z 1 aut. @Z 3 aut.`
A14	`02`			`@1 Laboratoire des Sciences du Génie, Chimique, CNRS-ENSIC-INPL, BP 451 @2 54001 Nancy @3 FRA @Z 2 aut.`
A14	`03`			`@1 Department of Animal Sciences, University of Illinois at Urbana-Champaign, Animal Sciences Laboratory, 1207 West Gregory Drive @2 Urbana, IL 61801 @3 USA @Z 3 aut.`
A20				`@1 456-468`
A21				`@1 2005`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 8940A @5 354000126115480200`
A44				`@0 0000 @1 © 2005 INIST-CNRS. All rights reserved.`
A45				`@0 1 p.1/4`
A47	`01`	`1`		`@0 05-0102743`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Water research : (Oxford)`
A66	`01`			`@0 GBR`
C01	`01`		`ENG`	@0 Microscopic techniques ranging from epifluorescence microscopy to confocal laser scanning microscopy (CLSM) and two photon excitation laser scanning microscopy (TPE-LSM) combined with fluorescent stains can help to evaluate complex microbial aggregates such as activated sludge flocs. To determine the application limits of these microscopic techniques, activated sludge samples from three different sources were evaluated after staining with a fluorescent viability indicator (Baclight Bacterial Viability Kit, Molecular Probes). Image analysis routines were developed to quantify overall amounts of red and green stained cells, location of stained cells within the flocs, and the spatial organization in clusters and filaments. It was found that the selection of the appropriate microscopic technique depends strongly on the type of microbial aggregates being analyzed. For flocs with high cell density, the use of TPE-LSM is preferred, since it provides a clearer image of the internal structure of the aggregate. Epifluorescence microscopy did not allow to reliably quantify red stained cells in dense aggregates. CLSM did not adequately image the internal filamentous structure and the location of stained cells within dense flocs. However, for typical activated sludge flocs epifluorescence and CLSM proved adequate.
C02	`01`	`X`		`@0 001D16A05A`
C02	`02`	`X`		`@0 002A31D07A`
C02	`03`	`X`		`@0 215`
C03	`01`	`X`	`FRE`	`@0 Epuration eau usée @5 01`
C03	`01`	`X`	`ENG`	`@0 Waste water purification @5 01`
C03	`01`	`X`	`SPA`	`@0 Depuración aguas servidas @5 01`
C03	`02`	`X`	`FRE`	`@0 Epuration biologique @5 02`
C03	`02`	`X`	`ENG`	`@0 Biological purification @5 02`
C03	`02`	`X`	`SPA`	`@0 Depuración biológica @5 02`
C03	`03`	`X`	`FRE`	`@0 Boue activée @5 03`
C03	`03`	`X`	`ENG`	`@0 Activated sludge @5 03`
C03	`03`	`X`	`SPA`	`@0 Lodo activado @5 03`
C03	`04`	`X`	`FRE`	`@0 Floc @5 04`
C03	`04`	`X`	`ENG`	`@0 Flock @5 04`
C03	`04`	`X`	`SPA`	`@0 Borla @5 04`
C03	`05`	`X`	`FRE`	`@0 Analyse image @5 05`
C03	`05`	`X`	`ENG`	`@0 Image analysis @5 05`
C03	`05`	`X`	`SPA`	`@0 Análisis imagen @5 05`
C03	`06`	`X`	`FRE`	`@0 Analyse structurale @5 06`
C03	`06`	`X`	`ENG`	`@0 Structural analysis @5 06`
C03	`06`	`X`	`SPA`	`@0 Análisis estructural @5 06`
C03	`07`	`X`	`FRE`	`@0 Agrégat @5 07`
C03	`07`	`X`	`ENG`	`@0 Aggregate @5 07`
C03	`07`	`X`	`SPA`	`@0 Agregado @5 07`
C03	`08`	`X`	`FRE`	`@0 Analyse quantitative @5 08`
C03	`08`	`X`	`ENG`	`@0 Quantitative analysis @5 08`
C03	`08`	`X`	`SPA`	`@0 Análisis cuantitativo @5 08`
C03	`09`	`X`	`FRE`	`@0 Microscopie épifluorescence @5 09`
C03	`09`	`X`	`ENG`	`@0 Epifluorescence microscopy @5 09`
C03	`09`	`X`	`SPA`	`@0 Microscopía epifluorescencia @5 09`
C03	`10`	`X`	`FRE`	`@0 Microscopie confocale @5 10`
C03	`10`	`X`	`ENG`	`@0 Confocal microscopy @5 10`
C03	`10`	`X`	`SPA`	`@0 Microscopía confocal @5 10`
C03	`11`	`X`	`FRE`	`@0 Microscope laser @5 11`
C03	`11`	`X`	`ENG`	`@0 Laser microscope @5 11`
C03	`11`	`X`	`SPA`	`@0 Microscopio láser @5 11`
C03	`12`	`X`	`FRE`	`@0 Microscope balayage @5 12`
C03	`12`	`X`	`ENG`	`@0 Scanning microscope @5 12`
C03	`12`	`X`	`SPA`	`@0 Microscopio barrido @5 12`
C03	`13`	`X`	`FRE`	`@0 Excitation 2 photons @5 13`
C03	`13`	`X`	`ENG`	`@0 Two photon excitation @5 13`
C03	`13`	`X`	`SPA`	`@0 Excitación 2 fotones @5 13`
N21				`@1 066`
N44	`01`			`@1 PSI`
N82				`@1 PSI`

Format Inist (serveur)

NO :	PASCAL 05-0102743 INIST
ET :	Evaluation of microscopic techniques (epifluorescence microscopy, CLSM, TPE-LSM) as a basis for the quantitative image analysis of activated sludge
AU :	LOPEZ (C.); PONS (M. N.); MORGENROTH (E.)
AF :	Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 3219 Newmark Civil Engineering Laboratory, 250, 205 North Mathews Avenue/Urbana, IL 61801/Etats-Unis (1 aut., 3 aut.); Laboratoire des Sciences du Génie, Chimique, CNRS-ENSIC-INPL, BP 451/54001 Nancy/France (2 aut.); Department of Animal Sciences, University of Illinois at Urbana-Champaign, Animal Sciences Laboratory, 1207 West Gregory Drive/Urbana, IL 61801/Etats-Unis (3 aut.)
DT :	Publication en série; Niveau analytique
SO :	Water research : (Oxford); ISSN 0043-1354; Coden WATRAG; Royaume-Uni; Da. 2005; Vol. 39; No. 2-3; Pp. 456-468; Bibl. 1 p.1/4
LA :	Anglais
EA :	Microscopic techniques ranging from epifluorescence microscopy to confocal laser scanning microscopy (CLSM) and two photon excitation laser scanning microscopy (TPE-LSM) combined with fluorescent stains can help to evaluate complex microbial aggregates such as activated sludge flocs. To determine the application limits of these microscopic techniques, activated sludge samples from three different sources were evaluated after staining with a fluorescent viability indicator (Baclight Bacterial Viability Kit, Molecular Probes). Image analysis routines were developed to quantify overall amounts of red and green stained cells, location of stained cells within the flocs, and the spatial organization in clusters and filaments. It was found that the selection of the appropriate microscopic technique depends strongly on the type of microbial aggregates being analyzed. For flocs with high cell density, the use of TPE-LSM is preferred, since it provides a clearer image of the internal structure of the aggregate. Epifluorescence microscopy did not allow to reliably quantify red stained cells in dense aggregates. CLSM did not adequately image the internal filamentous structure and the location of stained cells within dense flocs. However, for typical activated sludge flocs epifluorescence and CLSM proved adequate.
CC :	001D16A05A; 002A31D07A; 215
FD :	Epuration eau usée; Epuration biologique; Boue activée; Floc; Analyse image; Analyse structurale; Agrégat; Analyse quantitative; Microscopie épifluorescence; Microscopie confocale; Microscope laser; Microscope balayage; Excitation 2 photons
ED :	Waste water purification; Biological purification; Activated sludge; Flock; Image analysis; Structural analysis; Aggregate; Quantitative analysis; Epifluorescence microscopy; Confocal microscopy; Laser microscope; Scanning microscope; Two photon excitation
SD :	Depuración aguas servidas; Depuración biológica; Lodo activado; Borla; Análisis imagen; Análisis estructural; Agregado; Análisis cuantitativo; Microscopía epifluorescencia; Microscopía confocal; Microscopio láser; Microscopio barrido; Excitación 2 fotones
LO :	INIST-8940A.354000126115480200
ID :	05-0102743

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Pascal:05-0102743

Le document en format XML

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<front><div type="abstract" xml:lang="en">Microscopic techniques ranging from epifluorescence microscopy to confocal laser scanning microscopy (CLSM) and two photon excitation laser scanning microscopy (TPE-LSM) combined with fluorescent stains can help to evaluate complex microbial aggregates such as activated sludge flocs. To determine the application limits of these microscopic techniques, activated sludge samples from three different sources were evaluated after staining with a fluorescent viability indicator (Baclight Bacterial Viability Kit, Molecular Probes). Image analysis routines were developed to quantify overall amounts of red and green stained cells, location of stained cells within the flocs, and the spatial organization in clusters and filaments. It was found that the selection of the appropriate microscopic technique depends strongly on the type of microbial aggregates being analyzed. For flocs with high cell density, the use of TPE-LSM is preferred, since it provides a clearer image of the internal structure of the aggregate. Epifluorescence microscopy did not allow to reliably quantify red stained cells in dense aggregates. CLSM did not adequately image the internal filamentous structure and the location of stained cells within dense flocs. However, for typical activated sludge flocs epifluorescence and CLSM proved adequate.</div>
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<fC01 i1="01" l="ENG"><s0>Microscopic techniques ranging from epifluorescence microscopy to confocal laser scanning microscopy (CLSM) and two photon excitation laser scanning microscopy (TPE-LSM) combined with fluorescent stains can help to evaluate complex microbial aggregates such as activated sludge flocs. To determine the application limits of these microscopic techniques, activated sludge samples from three different sources were evaluated after staining with a fluorescent viability indicator (Baclight Bacterial Viability Kit, Molecular Probes). Image analysis routines were developed to quantify overall amounts of red and green stained cells, location of stained cells within the flocs, and the spatial organization in clusters and filaments. It was found that the selection of the appropriate microscopic technique depends strongly on the type of microbial aggregates being analyzed. For flocs with high cell density, the use of TPE-LSM is preferred, since it provides a clearer image of the internal structure of the aggregate. Epifluorescence microscopy did not allow to reliably quantify red stained cells in dense aggregates. CLSM did not adequately image the internal filamentous structure and the location of stained cells within dense flocs. However, for typical activated sludge flocs epifluorescence and CLSM proved adequate.</s0>
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<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Agregado</s0>
<s5>07</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Analyse quantitative</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Quantitative analysis</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Análisis cuantitativo</s0>
<s5>08</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Microscopie épifluorescence</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Epifluorescence microscopy</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Microscopía epifluorescencia</s0>
<s5>09</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Microscopie confocale</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Confocal microscopy</s0>
<s5>10</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Microscopía confocal</s0>
<s5>10</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Microscope laser</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Laser microscope</s0>
<s5>11</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Microscopio láser</s0>
<s5>11</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Microscope balayage</s0>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Scanning microscope</s0>
<s5>12</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Microscopio barrido</s0>
<s5>12</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Excitation 2 photons</s0>
<s5>13</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Two photon excitation</s0>
<s5>13</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Excitación 2 fotones</s0>
<s5>13</s5>
</fC03>
<fN21><s1>066</s1>
</fN21>
<fN44 i1="01"><s1>PSI</s1>
</fN44>
<fN82><s1>PSI</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 05-0102743 INIST</NO>
<ET>Evaluation of microscopic techniques (epifluorescence microscopy, CLSM, TPE-LSM) as a basis for the quantitative image analysis of activated sludge</ET>
<AU>LOPEZ (C.); PONS (M. N.); MORGENROTH (E.)</AU>
<AF>Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 3219 Newmark Civil Engineering Laboratory, 250, 205 North Mathews Avenue/Urbana, IL 61801/Etats-Unis (1 aut., 3 aut.); Laboratoire des Sciences du Génie, Chimique, CNRS-ENSIC-INPL, BP 451/54001 Nancy/France (2 aut.); Department of Animal Sciences, University of Illinois at Urbana-Champaign, Animal Sciences Laboratory, 1207 West Gregory Drive/Urbana, IL 61801/Etats-Unis (3 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Water research : (Oxford); ISSN 0043-1354; Coden WATRAG; Royaume-Uni; Da. 2005; Vol. 39; No. 2-3; Pp. 456-468; Bibl. 1 p.1/4</SO>
<LA>Anglais</LA>
<EA>Microscopic techniques ranging from epifluorescence microscopy to confocal laser scanning microscopy (CLSM) and two photon excitation laser scanning microscopy (TPE-LSM) combined with fluorescent stains can help to evaluate complex microbial aggregates such as activated sludge flocs. To determine the application limits of these microscopic techniques, activated sludge samples from three different sources were evaluated after staining with a fluorescent viability indicator (Baclight Bacterial Viability Kit, Molecular Probes). Image analysis routines were developed to quantify overall amounts of red and green stained cells, location of stained cells within the flocs, and the spatial organization in clusters and filaments. It was found that the selection of the appropriate microscopic technique depends strongly on the type of microbial aggregates being analyzed. For flocs with high cell density, the use of TPE-LSM is preferred, since it provides a clearer image of the internal structure of the aggregate. Epifluorescence microscopy did not allow to reliably quantify red stained cells in dense aggregates. CLSM did not adequately image the internal filamentous structure and the location of stained cells within dense flocs. However, for typical activated sludge flocs epifluorescence and CLSM proved adequate.</EA>
<CC>001D16A05A; 002A31D07A; 215</CC>
<FD>Epuration eau usée; Epuration biologique; Boue activée; Floc; Analyse image; Analyse structurale; Agrégat; Analyse quantitative; Microscopie épifluorescence; Microscopie confocale; Microscope laser; Microscope balayage; Excitation 2 photons</FD>
<ED>Waste water purification; Biological purification; Activated sludge; Flock; Image analysis; Structural analysis; Aggregate; Quantitative analysis; Epifluorescence microscopy; Confocal microscopy; Laser microscope; Scanning microscope; Two photon excitation</ED>
<SD>Depuración aguas servidas; Depuración biológica; Lodo activado; Borla; Análisis imagen; Análisis estructural; Agregado; Análisis cuantitativo; Microscopía epifluorescencia; Microscopía confocal; Microscopio láser; Microscopio barrido; Excitación 2 fotones</SD>
<LO>INIST-8940A.354000126115480200</LO>
<ID>05-0102743</ID>
</server>
</inist>
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Evaluation of microscopic techniques (epifluorescence microscopy, CLSM, TPE-LSM) as a basis for the quantitative image analysis of activated sludge

Evaluation of microscopic techniques (epifluorescence microscopy, CLSM, TPE-LSM) as a basis for the quantitative image analysis of activated sludge

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