Protein encapsulation in biodegradable amphiphilic microspheres
Identifieur interne : 000A86 ( PascalFrancis/Checkpoint ); précédent : 000A85; suivant : 000A87Protein encapsulation in biodegradable amphiphilic microspheres
Auteurs : P. Bouillot [France] ; N. Ubrich [France] ; F. Sommer [France] ; TRAN MINH DUC [France] ; J.-P. Loeffler [France] ; Édith Dellacherie [France]Source :
- International journal of pharmaceutics [ 0378-5173 ] ; 1999.
Descripteurs français
- Pascal (Inist)
- Technologie pharmaceutique, Forme pharmaceutique, Microsphère, Sérumalbumine, Bovin, Vecteur médicament, Polymère vecteur, Adsorption, Libération, Principe actif, In vitro, Polymère amphiphile, Lactique acide polymère, Ester polymère, Copolymère biséquencé, Teneur eau, Encapsulation, Ethylène oxyde copolymère, Emulsion eau huile eau, Copolymère triséquencé.
- Wicri :
- topic : Bovin.
English descriptors
- KwdEn :
- Active ingredient, Adsorption, Amphiphilic polymer, Bovine, Control release polymer, Diblock copolymer, Dosage form, Drug carrier, Encapsulation, Ester polymer, Ethylene oxide copolymer, In vitro, Lactic acid polymer, Microsphere, Pharmaceutical technology, Release, Serum albumin, Triblock copolymer, Water content, Water oil water emulsion.
Abstract
MPOE-PLA microspheres containing bovine serum albumin (BSA) were prepared by the double emulsion method with high encapsulation efficiency (∼ 93%). Confocal scanning microscopic analysis using MPOE-PLA labelled with 1-pyrenemethanol showed the MPOE coating of the microsphere surface. This coating improves the performance of the release system compared with PLA microspheres; the hydrophilic chains reduce the BSA adsorption onto the microspheres and increase the amount of BSA released in the supernatant. Microsphere analysis using atomic force microscopy showed that the presence of the MPOE chains also leads to surface roughness. Studies of the diffusion of 1% rhodamine aqueous solution into the microspheres by means of confocal microscopy showed a fast diffusion of water through the matrices containing high molecular weight MPOE chains ( > 10000 g mol- ') and could explain the fast release of BSA from these microspheres.
Affiliations:
- France
- Alsace (région administrative), Grand Est, Lorraine (région), Provence-Alpes-Côte d'Azur
- Marseilles, Nancy, Strasbourg
- Centre national de la recherche scientifique, Laboratoire réactions et génie des procédés, Université Strasbourg 1, Université de Lorraine
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Pascal:99-0311903Le document en format XML
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Bouillot</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratoire de Chimie-Physique Macromoléculaire, UMR CNRS-INPL 7568, groupe ENSIC, BP 451</s1><s2>54 001 Nancy</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>54 001 Nancy</wicri:noRegion><wicri:noRegion>BP 451</wicri:noRegion><wicri:noRegion>54 001 Nancy</wicri:noRegion></affiliation></author><author><name sortKey="Ubrich, N" sort="Ubrich, N" uniqKey="Ubrich N" first="N." last="Ubrich">N. Ubrich</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Laboratoire de Pharmacie Galénique et Biopharmacie, Faculté des Sciences Pharmaceutiques et Biologiques, 5 rue Albert Lebrun, BP 403</s1><s2>54 001 Nancy</s2><s3>FRA</s3><sZ>2 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>54 001 Nancy</wicri:noRegion><wicri:noRegion>BP 403</wicri:noRegion><wicri:noRegion>54 001 Nancy</wicri:noRegion></affiliation></author><author><name sortKey="Sommer, F" sort="Sommer, F" uniqKey="Sommer F" first="F." last="Sommer">F. Sommer</name><affiliation wicri:level="3"><inist:fA14 i1="03"><s1>Biophy, 6 rue Anne Gacon, Village des Entreprises de Saint Henri</s1><s2>13016 Marseilles</s2><s3>FRA</s3><sZ>3 aut.</sZ></inist:fA14><country>France</country><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseilles</settlement></placeName></affiliation></author><author><name sortKey="Tran Minh Duc" sort="Tran Minh Duc" uniqKey="Tran Minh Duc" last="Tran Minh Duc">TRAN MINH DUC</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Université Claude Bernard</s1><s2>69 630 Villeurbanne</s2><s3>FRA</s3><sZ>4 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>69 630 Villeurbanne</wicri:noRegion><wicri:noRegion>Université Claude Bernard</wicri:noRegion><wicri:noRegion>Université Claude Bernard</wicri:noRegion></affiliation></author><author><name sortKey="Loeffler, J P" sort="Loeffler, J P" uniqKey="Loeffler J" first="J.-P." last="Loeffler">J.-P. Loeffler</name><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Laboratoire de neurophysiologie cellulaire et intégrée, Université Louis Pasteur, 21 rue Descartes</s1><s2>67 084 Strasbourg</s2><s3>FRA</s3><sZ>5 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>67 084 Strasbourg</wicri:noRegion><wicri:noRegion>21 rue Descartes</wicri:noRegion><wicri:noRegion>67 084 Strasbourg</wicri:noRegion><orgName type="university">Université Strasbourg 1</orgName><placeName><settlement type="city">Strasbourg</settlement><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region></placeName></affiliation></author><author><name sortKey="Dellacherie, E" sort="Dellacherie, E" uniqKey="Dellacherie E" first="E." last="Dellacherie">Édith Dellacherie</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Laboratoire de Chimie-Physique Macromoléculaire, UMR CNRS-INPL 7568, groupe ENSIC, BP 451</s1><s2>54 001 Nancy</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>France</country><wicri:noRegion>54 001 Nancy</wicri:noRegion><wicri:noRegion>BP 451</wicri:noRegion><wicri:noRegion>54 001 Nancy</wicri:noRegion><placeName><settlement type="city">Nancy</settlement><region type="region" nuts="2">Grand Est</region><region type="region" nuts="2">Lorraine (région)</region></placeName><orgName type="laboratoire" n="5">Laboratoire réactions et génie des procédés</orgName><orgName type="university">Université de Lorraine</orgName><orgName type="institution">Centre national de la recherche scientifique</orgName></affiliation></author></analytic><series><title level="j" type="main">International journal of pharmaceutics</title><title level="j" type="abbreviated">Int. j. pharm.</title><idno type="ISSN">0378-5173</idno><imprint><date when="1999">1999</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">International journal of pharmaceutics</title><title level="j" type="abbreviated">Int. j. pharm.</title><idno type="ISSN">0378-5173</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Active ingredient</term><term>Adsorption</term><term>Amphiphilic polymer</term><term>Bovine</term><term>Control release polymer</term><term>Diblock copolymer</term><term>Dosage form</term><term>Drug carrier</term><term>Encapsulation</term><term>Ester polymer</term><term>Ethylene oxide copolymer</term><term>In vitro</term><term>Lactic acid polymer</term><term>Microsphere</term><term>Pharmaceutical technology</term><term>Release</term><term>Serum albumin</term><term>Triblock copolymer</term><term>Water content</term><term>Water oil water emulsion</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Technologie pharmaceutique</term><term>Forme pharmaceutique</term><term>Microsphère</term><term>Sérumalbumine</term><term>Bovin</term><term>Vecteur médicament</term><term>Polymère vecteur</term><term>Adsorption</term><term>Libération</term><term>Principe actif</term><term>In vitro</term><term>Polymère amphiphile</term><term>Lactique acide polymère</term><term>Ester polymère</term><term>Copolymère biséquencé</term><term>Teneur eau</term><term>Encapsulation</term><term>Ethylène oxyde copolymère</term><term>Emulsion eau huile eau</term><term>Copolymère triséquencé</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Bovin</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">MPOE-PLA microspheres containing bovine serum albumin (BSA) were prepared by the double emulsion method with high encapsulation efficiency (∼ 93%). Confocal scanning microscopic analysis using MPOE-PLA labelled with 1-pyrenemethanol showed the MPOE coating of the microsphere surface. This coating improves the performance of the release system compared with PLA microspheres; the hydrophilic chains reduce the BSA adsorption onto the microspheres and increase the amount of BSA released in the supernatant. Microsphere analysis using atomic force microscopy showed that the presence of the MPOE chains also leads to surface roughness. Studies of the diffusion of 1% rhodamine aqueous solution into the microspheres by means of confocal microscopy showed a fast diffusion of water through the matrices containing high molecular weight MPOE chains ( > 10000 g mol<sup>-</sup> ') and could explain the fast release of BSA from these microspheres.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>0378-5173</s0></fA01><fA02 i1="01"><s0>IJPHDE</s0></fA02><fA03 i2="1"><s0>Int. j. pharm.</s0></fA03><fA05><s2>181</s2></fA05><fA06><s2>2</s2></fA06><fA08 i1="01" i2="1" l="ENG"><s1>Protein encapsulation in biodegradable amphiphilic microspheres</s1></fA08><fA11 i1="01" i2="1"><s1>BOUILLOT (P.)</s1></fA11><fA11 i1="02" i2="1"><s1>UBRICH (N.)</s1></fA11><fA11 i1="03" i2="1"><s1>SOMMER (F.)</s1></fA11><fA11 i1="04" i2="1"><s1>TRAN MINH DUC</s1></fA11><fA11 i1="05" i2="1"><s1>LOEFFLER (J.-P.)</s1></fA11><fA11 i1="06" i2="1"><s1>DELLACHERIE (E.)</s1></fA11><fA14 i1="01"><s1>Laboratoire de Chimie-Physique Macromoléculaire, UMR CNRS-INPL 7568, groupe ENSIC, BP 451</s1><s2>54 001 Nancy</s2><s3>FRA</s3><sZ>1 aut.</sZ><sZ>6 aut.</sZ></fA14><fA14 i1="02"><s1>Laboratoire de Pharmacie Galénique et Biopharmacie, Faculté des Sciences Pharmaceutiques et Biologiques, 5 rue Albert Lebrun, BP 403</s1><s2>54 001 Nancy</s2><s3>FRA</s3><sZ>2 aut.</sZ></fA14><fA14 i1="03"><s1>Biophy, 6 rue Anne Gacon, Village des Entreprises de Saint Henri</s1><s2>13016 Marseilles</s2><s3>FRA</s3><sZ>3 aut.</sZ></fA14><fA14 i1="04"><s1>Université Claude Bernard</s1><s2>69 630 Villeurbanne</s2><s3>FRA</s3><sZ>4 aut.</sZ></fA14><fA14 i1="05"><s1>Laboratoire de neurophysiologie cellulaire et intégrée, Université Louis Pasteur, 21 rue Descartes</s1><s2>67 084 Strasbourg</s2><s3>FRA</s3><sZ>5 aut.</sZ></fA14><fA20><s1>159-172</s1></fA20><fA21><s1>1999</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>16510</s2><s5>354000085396610030</s5></fA43><fA44><s0>0000</s0><s1>© 1999 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>1 p.1/4</s0></fA45><fA47 i1="01" i2="1"><s0>99-0311903</s0></fA47><fA60><s1>P</s1><s3>PR</s3></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>International journal of pharmaceutics</s0></fA64><fA66 i1="01"><s0>NLD</s0></fA66><fC01 i1="01" l="ENG"><s0>MPOE-PLA microspheres containing bovine serum albumin (BSA) were prepared by the double emulsion method with high encapsulation efficiency (∼ 93%). Confocal scanning microscopic analysis using MPOE-PLA labelled with 1-pyrenemethanol showed the MPOE coating of the microsphere surface. This coating improves the performance of the release system compared with PLA microspheres; the hydrophilic chains reduce the BSA adsorption onto the microspheres and increase the amount of BSA released in the supernatant. Microsphere analysis using atomic force microscopy showed that the presence of the MPOE chains also leads to surface roughness. Studies of the diffusion of 1% rhodamine aqueous solution into the microspheres by means of confocal microscopy showed a fast diffusion of water through the matrices containing high molecular weight MPOE chains ( > 10000 g mol<sup>-</sup> ') and could explain the fast release of BSA from these microspheres.</s0></fC01><fC02 i1="01" i2="X"><s0>002B02A03</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Technologie pharmaceutique</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Pharmaceutical technology</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Tecnología farmacéutica</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Forme pharmaceutique</s0><s5>04</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Dosage form</s0><s5>04</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Forma farmacéutica</s0><s5>04</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Microsphère</s0><s5>07</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Microsphere</s0><s5>07</s5></fC03><fC03 i1="03" i2="X" 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l="ENG"><s0>Adsorption</s0><s5>16</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Adsorción</s0><s5>16</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Libération</s0><s5>17</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Release</s0><s5>17</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Liberación</s0><s5>17</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Principe actif</s0><s5>18</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Active ingredient</s0><s5>18</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Principio activo</s0><s5>18</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>In vitro</s0><s5>19</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>In vitro</s0><s5>19</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>In vitro</s0><s5>19</s5></fC03><fC03 i1="12" i2="X" l="FRE"><s0>Polymère amphiphile</s0><s5>20</s5></fC03><fC03 i1="12" i2="X" l="ENG"><s0>Amphiphilic polymer</s0><s5>20</s5></fC03><fC03 i1="12" i2="X" l="SPA"><s0>Polímero amfifilo</s0><s5>20</s5></fC03><fC03 i1="13" i2="X" l="FRE"><s0>Lactique acide polymère</s0><s2>NK</s2><s5>21</s5></fC03><fC03 i1="13" i2="X" l="ENG"><s0>Lactic acid polymer</s0><s2>NK</s2><s5>21</s5></fC03><fC03 i1="13" i2="X" l="SPA"><s0>Láctico ácido polímero</s0><s2>NK</s2><s5>21</s5></fC03><fC03 i1="14" i2="X" l="FRE"><s0>Ester polymère</s0><s2>NK</s2><s5>22</s5></fC03><fC03 i1="14" i2="X" l="ENG"><s0>Ester polymer</s0><s2>NK</s2><s5>22</s5></fC03><fC03 i1="14" i2="X" l="SPA"><s0>Ester polímero</s0><s2>NK</s2><s5>22</s5></fC03><fC03 i1="15" i2="X" l="FRE"><s0>Copolymère biséquencé</s0><s2>NK</s2><s5>23</s5></fC03><fC03 i1="15" i2="X" l="ENG"><s0>Diblock copolymer</s0><s2>NK</s2><s5>23</s5></fC03><fC03 i1="15" i2="X" l="SPA"><s0>Copolímero bisecuencia</s0><s2>NK</s2><s5>23</s5></fC03><fC03 i1="16" i2="X" l="FRE"><s0>Teneur eau</s0><s5>24</s5></fC03><fC03 i1="16" i2="X" l="ENG"><s0>Water content</s0><s5>24</s5></fC03><fC03 i1="16" i2="X" l="SPA"><s0>Dosis agua</s0><s5>24</s5></fC03><fC03 i1="17" i2="X" l="FRE"><s0>Encapsulation</s0><s5>35</s5></fC03><fC03 i1="17" i2="X" l="ENG"><s0>Encapsulation</s0><s5>35</s5></fC03><fC03 i1="17" i2="X" l="SPA"><s0>Encapsulación</s0><s5>35</s5></fC03><fC03 i1="18" i2="X" l="FRE"><s0>Ethylène oxyde copolymère</s0><s2>NK</s2><s5>78</s5></fC03><fC03 i1="18" i2="X" l="ENG"><s0>Ethylene oxide copolymer</s0><s2>NK</s2><s5>78</s5></fC03><fC03 i1="18" i2="X" l="SPA"><s0>Etileno óxido copolímero</s0><s2>NK</s2><s5>78</s5></fC03><fC03 i1="19" i2="X" l="FRE"><s0>Emulsion eau huile eau</s0><s5>79</s5></fC03><fC03 i1="19" i2="X" l="ENG"><s0>Water oil water emulsion</s0><s5>79</s5></fC03><fC03 i1="19" i2="X" l="SPA"><s0>Emulsión agua aceite agua</s0><s5>79</s5></fC03><fC03 i1="20" i2="X" l="FRE"><s0>Copolymère triséquencé</s0><s2>NK</s2><s5>80</s5></fC03><fC03 i1="20" i2="X" l="ENG"><s0>Triblock copolymer</s0><s2>NK</s2><s5>80</s5></fC03><fC03 i1="20" i2="X" l="SPA"><s0>Copolímero trisecuencia</s0><s2>NK</s2><s5>80</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Artiodactyla</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Artiodactyla</s0><s2>NS</s2></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Artiodactyla</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Ungulata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Ungulata</s0><s2>NS</s2></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Ungulata</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="FRE"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="ENG"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="03" i2="X" l="SPA"><s0>Mammalia</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="FRE"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="ENG"><s0>Vertebrata</s0><s2>NS</s2></fC07><fC07 i1="04" i2="X" l="SPA"><s0>Vertebrata</s0><s2>NS</s2></fC07><fN21><s1>193</s1></fN21></pA></standard></inist><affiliations><list><country><li>France</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li><li>Lorraine (région)</li><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseilles</li><li>Nancy</li><li>Strasbourg</li></settlement><orgName><li>Centre national de la recherche scientifique</li><li>Laboratoire réactions et génie des procédés</li><li>Université Strasbourg 1</li><li>Université de Lorraine</li></orgName></list><tree><country name="France"><noRegion><name sortKey="Bouillot, P" sort="Bouillot, P" uniqKey="Bouillot P" first="P." last="Bouillot">P. Bouillot</name></noRegion><name sortKey="Dellacherie, E" sort="Dellacherie, E" uniqKey="Dellacherie E" first="E." last="Dellacherie">Édith Dellacherie</name><name sortKey="Loeffler, J P" sort="Loeffler, J P" uniqKey="Loeffler J" first="J.-P." last="Loeffler">J.-P. Loeffler</name><name sortKey="Sommer, F" sort="Sommer, F" uniqKey="Sommer F" first="F." last="Sommer">F. Sommer</name><name sortKey="Tran Minh Duc" sort="Tran Minh Duc" uniqKey="Tran Minh Duc" last="Tran Minh Duc">TRAN MINH DUC</name><name sortKey="Ubrich, N" sort="Ubrich, N" uniqKey="Ubrich N" first="N." last="Ubrich">N. Ubrich</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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