LrgpV1, Main, Merge, bibRecord, 001954

Kinetics and metabolic specificities of Vero cells in bioreactor cultures with serum-free medium

Identifieur interne : 001954 ( Main/Merge ); précédent : 001953; suivant : 001955

Kinetics and metabolic specificities of Vero cells in bioreactor cultures with serum-free medium

Auteurs : Sébastien Quesney [France] ; Annie Marc [France] ; Catherine Gerdil [France] ; Cyrille Gimenez [France] ; Jacqueline Marvel [France] ; Yves Richard [France] ; Bernard Meignier [France]

Source :

Cytotechnology : (Dordrecht) [ 0920-9069 ] ; 2003.

RBID : Pascal:04-0217040

Descripteurs français

Pascal (Inist)
- Cinétique, Métabolisme, Spécificité, Culture cellulaire, Bioréacteur, Milieu culture, Milieu sans sérum, Aminoacide, Glucose, Lactate, Singe, Rein, Lignée VERO.
Wicri :
- topic : Glucose, Singe.

English descriptors

KwdEn :
- Aminoacid, Bioreactor, Cell culture, Culture medium, Glucose, Kidney, Kinetics, Lactates, Metabolism, Monkey, Serum free medium, Specificity.

Abstract

The aim of this study was to understand the metabolism kinetics of Vero cells grown on microcarriers in bioreactors in serum-free medium (SFM). We sought to determine what nutrients are essential for Vero cells and how they are consumed. Contrary to glucose and to most of the amino acids, glutamine and serine were very quickly depleted in this medium and can be supposed to be responsible for cell apoptosis. Lactate and ammonium ions did not reach toxic levels for Vero cells. We payed more attention to the lactate metabolism. Usually we observed that after about 2 days lactate was consumed in serum-containing media, but its concentration plateaud in SFM. Moreover, the addition of serum in SFM provoked lactate consumption and the rate of glucose and glutamine consumption was twice as high as in the SFM not supplemented with serum. The depletion of glutamine and serine and the metabolic deviations leading to a shortage of intermediate products required for other metabolic pathways probably contribute to the lower cell yield and higher cell death rate in SFM.

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Pascal:04-0217040

Le document en format XML

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<author><name sortKey="Meignier, Bernard" sort="Meignier, Bernard" uniqKey="Meignier B" first="Bernard" last="Meignier">Bernard Meignier</name>
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<author><name sortKey="Gimenez, Cyrille" sort="Gimenez, Cyrille" uniqKey="Gimenez C" first="Cyrille" last="Gimenez">Cyrille Gimenez</name>
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<author><name sortKey="Marvel, Jacqueline" sort="Marvel, Jacqueline" uniqKey="Marvel J" first="Jacqueline" last="Marvel">Jacqueline Marvel</name>
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<region type="old region" nuts="2">Rhône-Alpes</region>
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<author><name sortKey="Richard, Yves" sort="Richard, Yves" uniqKey="Richard Y" first="Yves" last="Richard">Yves Richard</name>
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<sZ>6 aut.</sZ>
</inist:fA14>
<country>France</country>
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<region type="old region" nuts="2">Rhône-Alpes</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Meignier, Bernard" sort="Meignier, Bernard" uniqKey="Meignier B" first="Bernard" last="Meignier">Bernard Meignier</name>
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</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aminoacid</term>
<term>Bioreactor</term>
<term>Cell culture</term>
<term>Culture medium</term>
<term>Glucose</term>
<term>Kidney</term>
<term>Kinetics</term>
<term>Lactates</term>
<term>Metabolism</term>
<term>Monkey</term>
<term>Serum free medium</term>
<term>Specificity</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Cinétique</term>
<term>Métabolisme</term>
<term>Spécificité</term>
<term>Culture cellulaire</term>
<term>Bioréacteur</term>
<term>Milieu culture</term>
<term>Milieu sans sérum</term>
<term>Aminoacide</term>
<term>Glucose</term>
<term>Lactate</term>
<term>Singe</term>
<term>Rein</term>
<term>Lignée VERO</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Glucose</term>
<term>Singe</term>
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<front><div type="abstract" xml:lang="en">The aim of this study was to understand the metabolism kinetics of Vero cells grown on microcarriers in bioreactors in serum-free medium (SFM). We sought to determine what nutrients are essential for Vero cells and how they are consumed. Contrary to glucose and to most of the amino acids, glutamine and serine were very quickly depleted in this medium and can be supposed to be responsible for cell apoptosis. Lactate and ammonium ions did not reach toxic levels for Vero cells. We payed more attention to the lactate metabolism. Usually we observed that after about 2 days lactate was consumed in serum-containing media, but its concentration plateaud in SFM. Moreover, the addition of serum in SFM provoked lactate consumption and the rate of glucose and glutamine consumption was twice as high as in the SFM not supplemented with serum. The depletion of glutamine and serine and the metabolic deviations leading to a shortage of intermediate products required for other metabolic pathways probably contribute to the lower cell yield and higher cell death rate in SFM.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Auvergne-Rhône-Alpes</li>
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<tree><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Quesney, Sebastien" sort="Quesney, Sebastien" uniqKey="Quesney S" first="Sébastien" last="Quesney">Sébastien Quesney</name>
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<name sortKey="Gerdil, Catherine" sort="Gerdil, Catherine" uniqKey="Gerdil C" first="Catherine" last="Gerdil">Catherine Gerdil</name>
<name sortKey="Gimenez, Cyrille" sort="Gimenez, Cyrille" uniqKey="Gimenez C" first="Cyrille" last="Gimenez">Cyrille Gimenez</name>
<name sortKey="Marc, Annie" sort="Marc, Annie" uniqKey="Marc A" first="Annie" last="Marc">Annie Marc</name>
<name sortKey="Marvel, Jacqueline" sort="Marvel, Jacqueline" uniqKey="Marvel J" first="Jacqueline" last="Marvel">Jacqueline Marvel</name>
<name sortKey="Meignier, Bernard" sort="Meignier, Bernard" uniqKey="Meignier B" first="Bernard" last="Meignier">Bernard Meignier</name>
<name sortKey="Richard, Yves" sort="Richard, Yves" uniqKey="Richard Y" first="Yves" last="Richard">Yves Richard</name>
</country>
</tree>
</affiliations>
</record>

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Kinetics and metabolic specificities of Vero cells in bioreactor cultures with serum-free medium

Kinetics and metabolic specificities of Vero cells in bioreactor cultures with serum-free medium

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri