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Optical method for long‐term and large‐scale monitoring of spatial biofilm development

Identifieur interne : 001279 ( Main/Exploration ); précédent : 001278; suivant : 001280

Optical method for long‐term and large‐scale monitoring of spatial biofilm development

Auteurs : K. Milferstedt [États-Unis] ; M. Pons [France] ; E. Morgenroth [États-Unis]

Source :

RBID : ISTEX:E3B4D9891D1F48332E1D9CB0C9BACB49BD37418A

Descripteurs français

English descriptors

Abstract

A method was developed that allows biofilm monitoring on the square centimeter scale over extended periods of time. The method is based on image acquisition using a desktop scanner and subsequent image analysis. It was shown that results from grey level analysis are highly correlated with physical properties of the biofilm like average biomass and biofilm thickness. The scanner method was applied to monitor overall biofilm growth, detachment, and surface roughness during two 3 and 4 week long experiments. Two significantly different growth dynamics during the biofilm development could be identified, depending on the biofilm history. Surface roughness on transects in flow direction was always higher than on transects perpendicular to the flow, reflecting the anisotropic characteristics of biofilms growing in a flow field. © 2006 Wiley Periodicals, Inc.

Url:
DOI: 10.1002/bit.20893


Affiliations:


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<term>Average thickness</term>
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<term>Background images</term>
<term>Background subtraction</term>
<term>Bakke</term>
<term>Batch inoculation</term>
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<term>Community shift</term>
<term>Community shifts</term>
<term>Deionized water</term>
<term>Desktop scanner</term>
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<term>Detachment event</term>
<term>Development biotechnology</term>
<term>Different growth dynamics</term>
<term>Dilution water</term>
<term>Early stages</term>
<term>Error bars</term>
<term>Exponential function</term>
<term>Fungal hyphae</term>
<term>Generation data</term>
<term>Generation development</term>
<term>Generation slides</term>
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<term>Grey level</term>
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<term>Lower range</term>
<term>Magnetic resonance imaging</term>
<term>Microbiol</term>
<term>Microscopic images</term>
<term>Monitoring</term>
<term>National science foundation</term>
<term>Nutrient solution</term>
<term>Optic probe</term>
<term>Optical density</term>
<term>Optical method</term>
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<term>Outer wall</term>
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<term>Spatial resolution</term>
<term>Square centimeter scale</term>
<term>Standard errors</term>
<term>Stoodley</term>
<term>Surface roughness</term>
<term>Thickness variability</term>
<term>Time axis</term>
<term>Time lapse microscopy</term>
<term>Total solids</term>
<term>Total solids measurements</term>
<term>Transects</term>
<term>Vertical direction</term>
<term>Vertical directions</term>
<term>Vertical transects</term>
<term>Wide range</term>
<term>Wiley periodicals</term>
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<term>Analyse image</term>
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<term>Annular reactor</term>
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<term>Average thickness</term>
<term>Background image</term>
<term>Background images</term>
<term>Background subtraction</term>
<term>Bakke</term>
<term>Batch inoculation</term>
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<term>Biomass accumulation</term>
<term>Biomass concentration</term>
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<term>Biomass distributions</term>
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<term>Community shifts</term>
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<term>Desktop scanner</term>
<term>Detachment</term>
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<term>Development biotechnology</term>
<term>Different growth dynamics</term>
<term>Dilution water</term>
<term>Early stages</term>
<term>Error bars</term>
<term>Exponential function</term>
<term>Fungal hyphae</term>
<term>Generation data</term>
<term>Generation development</term>
<term>Generation slides</term>
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<term>Image acquisition</term>
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<term>Individual slides</term>
<term>Initial formation</term>
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<term>Light microscopy</term>
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<term>Linear regressions</term>
<term>Local biomass concentrations</term>
<term>Local thickness</term>
<term>Lower range</term>
<term>Magnetic resonance imaging</term>
<term>Microbiol</term>
<term>Microscopic images</term>
<term>National science foundation</term>
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<term>Outer wall</term>
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<term>Standard errors</term>
<term>Stoodley</term>
<term>Surface roughness</term>
<term>Thickness variability</term>
<term>Time axis</term>
<term>Time lapse microscopy</term>
<term>Total solids</term>
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<front>
<div type="abstract" xml:lang="en">A method was developed that allows biofilm monitoring on the square centimeter scale over extended periods of time. The method is based on image acquisition using a desktop scanner and subsequent image analysis. It was shown that results from grey level analysis are highly correlated with physical properties of the biofilm like average biomass and biofilm thickness. The scanner method was applied to monitor overall biofilm growth, detachment, and surface roughness during two 3 and 4 week long experiments. Two significantly different growth dynamics during the biofilm development could be identified, depending on the biofilm history. Surface roughness on transects in flow direction was always higher than on transects perpendicular to the flow, reflecting the anisotropic characteristics of biofilms growing in a flow field. © 2006 Wiley Periodicals, Inc.</div>
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