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The exposure of bacteria to CdTe-core quantum dots: the importance of surface chemistryon cytotoxicity

Identifieur interne : 000F24 ( Main/Exploration ); précédent : 000F23; suivant : 000F25

The exposure of bacteria to CdTe-core quantum dots: the importance of surface chemistryon cytotoxicity

Auteurs : Raphal Schneider [France] ; Ccile Wolpert [France] ; Hlne Guilloteau [France] ; Lavinia Balan [France] ; Jacques Lambert [France] ; Christophe Merlin [France]

Source :

RBID : ISTEX:305E9C359E5C0F19C5E2A862A10381E646D2F6F6

Descripteurs français

English descriptors

Abstract

A series of water-soluble CdTe-core quantum dots (QDs) with diameters below 5.0nm andfunctionalized at their surface with polar ligands such as thioglycolic acid (TGA) or thetripeptide glutathione (GSH) were synthesized and characterized by UVvis absorptionspectroscopy, their photoluminescence measurements, atomic force microscopy (AFM) andtransmission electron microscopy (TEM). Because cell elongations and growth inhibitionswere observed during labeling experiments, the cytotoxicity of CdTe-core QDs wasinvestigated. Using growth inhibition tests combining different bacterial strains withdifferent CdTe-core QDs, it was possible to demonstrate that the cytotoxicity ofQDs towards bacteria depends on exposure concentrations, surface chemistry andcoating, and that it varied with the strain considered. Growth inhibition testscarried out with heavy-metal-resistant bacteria, as well as ICP-AES analyses ofcadmium species released by CdTe@TGA QDs, demonstrated that the leakage ofCd2 is notthe main source of QD toxicity. Our study suggests that QD cytotoxicity is rather due to the formationof TeO2 and probably the existence of CdO formed by surface oxidation. In this respect, QDspossessing a CdO shell appeared very toxic.

Url:
DOI: 10.1088/0957-4484/20/22/225101


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<front>
<div type="abstract">A series of water-soluble CdTe-core quantum dots (QDs) with diameters below 5.0nm andfunctionalized at their surface with polar ligands such as thioglycolic acid (TGA) or thetripeptide glutathione (GSH) were synthesized and characterized by UVvis absorptionspectroscopy, their photoluminescence measurements, atomic force microscopy (AFM) andtransmission electron microscopy (TEM). Because cell elongations and growth inhibitionswere observed during labeling experiments, the cytotoxicity of CdTe-core QDs wasinvestigated. Using growth inhibition tests combining different bacterial strains withdifferent CdTe-core QDs, it was possible to demonstrate that the cytotoxicity ofQDs towards bacteria depends on exposure concentrations, surface chemistry andcoating, and that it varied with the strain considered. Growth inhibition testscarried out with heavy-metal-resistant bacteria, as well as ICP-AES analyses ofcadmium species released by CdTe@TGA QDs, demonstrated that the leakage ofCd2 is notthe main source of QD toxicity. Our study suggests that QD cytotoxicity is rather due to the formationof TeO2 and probably the existence of CdO formed by surface oxidation. In this respect, QDspossessing a CdO shell appeared very toxic.</div>
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