LrgpV1, Main, Exploration, bibRecord, 000E67

Bioproduction and Anticancer Activity of Biosurfactant Produced by the Dematiaceous Fungus Exophiala dermatitidis SK80

Identifieur interne : 000E67 ( Main/Exploration ); précédent : 000E66; suivant : 000E68

Bioproduction and Anticancer Activity of Biosurfactant Produced by the Dematiaceous Fungus Exophiala dermatitidis SK80

Auteurs : Paramaporn Chiewpattanakul [Thaïlande, France] ; Sirinet Phonnok [Thaïlande] ; Alain Durand [France] ; Emmanuelle Marie [France] ; BENJAMAS WONGSATAYANON THANOMSUB [Thaïlande]

Source :

Journal of microbiology and biotechnology [ 1017-7825 ] ; 2010.

RBID : Pascal:11-0051627

Descripteurs français

Pascal (Inist)
- Production, Anticancéreux, Agent surface biologique, Fungi, Exophiala, Monoglycéride, Microorganisme.
Wicri :
- topic : Production.

English descriptors

KwdEn :
- Antineoplastic agent, Biosurfactant, Exophiala, Fungi, Microorganism, Monoglyceride, Production.

Abstract

A new biosurfactant producer was isolated from palm-oil-contaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH₄NO₃, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30°C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.

Affiliations:

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000223
to stream PascalFrancis, to step Curation: 000888
to stream PascalFrancis, to step Checkpoint: 000269
to stream Main, to step Merge: 001046
to stream Main, to step Curation: 000E67

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Bioproduction and Anticancer Activity of Biosurfactant Produced by the Dematiaceous Fungus Exophiala dermatitidis SK80</title>
<author><name sortKey="Chiewpattanakul, Paramaporn" sort="Chiewpattanakul, Paramaporn" uniqKey="Chiewpattanakul P" first="Paramaporn" last="Chiewpattanakul">Paramaporn Chiewpattanakul</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Faculty of Medicine, Srinakharinwirot University</s1>
<s2>Bangkok 10110</s2>
<s3>THA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Thaïlande</country>
<wicri:noRegion>Bangkok 10110</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratoire de Chimie Physique Macromoléculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451</s1>
<s2>54001 Nancy</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Phonnok, Sirinet" sort="Phonnok, Sirinet" uniqKey="Phonnok S" first="Sirinet" last="Phonnok">Sirinet Phonnok</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Faculty of Medicine, Srinakharinwirot University</s1>
<s2>Bangkok 10110</s2>
<s3>THA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Thaïlande</country>
<wicri:noRegion>Bangkok 10110</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Durand, Alain" sort="Durand, Alain" uniqKey="Durand A" first="Alain" last="Durand">Alain Durand</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratoire de Chimie Physique Macromoléculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451</s1>
<s2>54001 Nancy</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Marie, Emmanuelle" sort="Marie, Emmanuelle" uniqKey="Marie E" first="Emmanuelle" last="Marie">Emmanuelle Marie</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratoire de Chimie Physique Macromoléculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451</s1>
<s2>54001 Nancy</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Benjamas Wongsatayanon Thanomsub" sort="Benjamas Wongsatayanon Thanomsub" uniqKey="Benjamas Wongsatayanon Thanomsub" last="Benjamas Wongsatayanon Thanomsub">BENJAMAS WONGSATAYANON THANOMSUB</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Faculty of Medicine, Srinakharinwirot University</s1>
<s2>Bangkok 10110</s2>
<s3>THA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Thaïlande</country>
<wicri:noRegion>Bangkok 10110</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">11-0051627</idno>
<date when="2010">2010</date>
<idno type="stanalyst">PASCAL 11-0051627 INIST</idno>
<idno type="RBID">Pascal:11-0051627</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000223</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000888</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000269</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000269</idno>
<idno type="wicri:doubleKey">1017-7825:2010:Chiewpattanakul P:bioproduction:and:anticancer</idno>
<idno type="wicri:Area/Main/Merge">001046</idno>
<idno type="wicri:Area/Main/Curation">000E67</idno>
<idno type="wicri:Area/Main/Exploration">000E67</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Bioproduction and Anticancer Activity of Biosurfactant Produced by the Dematiaceous Fungus Exophiala dermatitidis SK80</title>
<author><name sortKey="Chiewpattanakul, Paramaporn" sort="Chiewpattanakul, Paramaporn" uniqKey="Chiewpattanakul P" first="Paramaporn" last="Chiewpattanakul">Paramaporn Chiewpattanakul</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Faculty of Medicine, Srinakharinwirot University</s1>
<s2>Bangkok 10110</s2>
<s3>THA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Thaïlande</country>
<wicri:noRegion>Bangkok 10110</wicri:noRegion>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratoire de Chimie Physique Macromoléculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451</s1>
<s2>54001 Nancy</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Phonnok, Sirinet" sort="Phonnok, Sirinet" uniqKey="Phonnok S" first="Sirinet" last="Phonnok">Sirinet Phonnok</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Faculty of Medicine, Srinakharinwirot University</s1>
<s2>Bangkok 10110</s2>
<s3>THA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Thaïlande</country>
<wicri:noRegion>Bangkok 10110</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Durand, Alain" sort="Durand, Alain" uniqKey="Durand A" first="Alain" last="Durand">Alain Durand</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratoire de Chimie Physique Macromoléculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451</s1>
<s2>54001 Nancy</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Marie, Emmanuelle" sort="Marie, Emmanuelle" uniqKey="Marie E" first="Emmanuelle" last="Marie">Emmanuelle Marie</name>
<affiliation wicri:level="3"><inist:fA14 i1="02"><s1>Laboratoire de Chimie Physique Macromoléculaire CNRS-Nancy University, ENSIC, 1 rue Grandville, BP 20451</s1>
<s2>54001 Nancy</s2>
<s3>FRA</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region" nuts="2">Grand Est</region>
<region type="old region" nuts="2">Lorraine (région)</region>
<settlement type="city">Nancy</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Benjamas Wongsatayanon Thanomsub" sort="Benjamas Wongsatayanon Thanomsub" uniqKey="Benjamas Wongsatayanon Thanomsub" last="Benjamas Wongsatayanon Thanomsub">BENJAMAS WONGSATAYANON THANOMSUB</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology, Faculty of Medicine, Srinakharinwirot University</s1>
<s2>Bangkok 10110</s2>
<s3>THA</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Thaïlande</country>
<wicri:noRegion>Bangkok 10110</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of microbiology and biotechnology</title>
<title level="j" type="abbreviated">J. microbiol. biotechnol.</title>
<idno type="ISSN">1017-7825</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of microbiology and biotechnology</title>
<title level="j" type="abbreviated">J. microbiol. biotechnol.</title>
<idno type="ISSN">1017-7825</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antineoplastic agent</term>
<term>Biosurfactant</term>
<term>Exophiala</term>
<term>Fungi</term>
<term>Microorganism</term>
<term>Monoglyceride</term>
<term>Production</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Production</term>
<term>Anticancéreux</term>
<term>Agent surface biologique</term>
<term>Fungi</term>
<term>Exophiala</term>
<term>Monoglycéride</term>
<term>Microorganisme</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Production</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A new biosurfactant producer was isolated from palm-oil-contaminated soil and later identified through morphology and DNA sequencing as the yeast-like fungus Exophiala dermatitidis. Biosurfactant production was catalyzed by vegetable oil, supplemented with a basal medium. The culture conditions that provided the biosurfactant with the highest surface activity were found to be 5% palm oil with 0.08% NH<sub>4</sub>
NO<sub>3</sub>
, at a pH of 5.3, with shaking at 200 rpm, and a temperature of 30°C for a 14-day period of incubation. The biosurfactant was purified, in accordance with surfactant properties, by solvent fractionation using silica gel column chromatography. The chemical structure of the strongest surface-active compound was elucidated through the use of NMR and mass spectroscopy, and noted to be monoolein, which then went on to demonstrate antiproliferative activity against cervical cancer (HeLa) and leukemia (U937) cell lines in a dose-dependent manner. Interestingly, no cytotoxicity was observed with normal cells even when high concentrations were used. Cell and DNA morphological changes, in both cancer cell lines, were observed to be cell shrinkage, membrane blebbling, and DNA fragmentation.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
<li>Thaïlande</li>
</country>
<region><li>Grand Est</li>
<li>Lorraine (région)</li>
</region>
<settlement><li>Nancy</li>
</settlement>
</list>
<tree><country name="Thaïlande"><noRegion><name sortKey="Chiewpattanakul, Paramaporn" sort="Chiewpattanakul, Paramaporn" uniqKey="Chiewpattanakul P" first="Paramaporn" last="Chiewpattanakul">Paramaporn Chiewpattanakul</name>
</noRegion>
<name sortKey="Benjamas Wongsatayanon Thanomsub" sort="Benjamas Wongsatayanon Thanomsub" uniqKey="Benjamas Wongsatayanon Thanomsub" last="Benjamas Wongsatayanon Thanomsub">BENJAMAS WONGSATAYANON THANOMSUB</name>
<name sortKey="Phonnok, Sirinet" sort="Phonnok, Sirinet" uniqKey="Phonnok S" first="Sirinet" last="Phonnok">Sirinet Phonnok</name>
</country>
<country name="France"><region name="Grand Est"><name sortKey="Chiewpattanakul, Paramaporn" sort="Chiewpattanakul, Paramaporn" uniqKey="Chiewpattanakul P" first="Paramaporn" last="Chiewpattanakul">Paramaporn Chiewpattanakul</name>
</region>
<name sortKey="Durand, Alain" sort="Durand, Alain" uniqKey="Durand A" first="Alain" last="Durand">Alain Durand</name>
<name sortKey="Marie, Emmanuelle" sort="Marie, Emmanuelle" uniqKey="Marie E" first="Emmanuelle" last="Marie">Emmanuelle Marie</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Lorraine/explor/LrgpV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E67 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E67 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Lorraine
   |area=    LrgpV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:11-0051627
   |texte=   Bioproduction and Anticancer Activity of Biosurfactant Produced by the Dematiaceous Fungus Exophiala dermatitidis SK80
}}

This area was generated with Dilib version V0.6.32.
Data generation: Sat Nov 11 15:47:48 2017. Site generation: Wed Mar 6 23:31:34 2024

	Serveur d'exploration sur le LRGP
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le LRGP

Bioproduction and Anticancer Activity of Biosurfactant Produced by the Dematiaceous Fungus Exophiala dermatitidis SK80

Bioproduction and Anticancer Activity of Biosurfactant Produced by the Dematiaceous Fungus Exophiala dermatitidis SK80

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri