Hyaluronate‐alginate gel as a novel biomaterial: Mechanical properties and formation mechanism
Identifieur interne : 000212 ( Istex/Corpus ); précédent : 000211; suivant : 000213Hyaluronate‐alginate gel as a novel biomaterial: Mechanical properties and formation mechanism
Auteurs : Sandra Oerther ; Hervé Le Gall ; Elisabeth Payan ; François Lapicque ; Nathalie Presle ; Patrick Hubert ; Jean Dexheimer ; Patrick Netter ; Françoise LapicqueSource :
- Biotechnology and Bioengineering [ 0006-3592 ] ; 1999-04-20.
English descriptors
- KwdEn :
- Actual alginate concentration, Alginate, Alginate content, Alginate solutions, Alginic acid, Aqueous solution, Average temperature, Average weight, Bead, Beads, Biologie forestiere, Calcium, Calcium chloride medium, Calcium chloride solution, Calcium content, Calcium contents, Calcium diffusion, Calcium figure, Calcium ions, Calcium medium, Characteristic viscosity, Classical problem, Coefficient, Complementary error function, Confidence intervals, Continuous sphere, Dialysis cell, Diffusion coefficient, Diffusion coefficients, Diffusion phenomena, Diffusion rates, Divalent cations, Drug carrier, Experimental data, Experimental procedure, Experimental variations, First hour, Gelation, Gelation mechanism, Gelation time, Generalised model, Good agreement, Guluronic, Guluronic acid, Hardness, Hyaluronate, Hyaluronate content, Hyaluronate loss, Hyaluronate salts, Hyaluronate solutions, Hyaluronic, Hyaluronic acid, Infinite dilution, Instantaneous compliance, John wiley sons, Lower extent, Mechanical properties, Molecular weight, Objective function, Oerther, Physical properties, Polymer, Polysaccharide, Polysaccharide mixtures, Polysaccharidic chains, Preliminary experiments, Pure alginate, Relaxation period, Rheological, Rheological measurements, Rooster comb, Rooster comb hyaluronate, Saturation chamber, Significant loss, Simple model, Small pores, Sodium alginate, Sodium hyaluronate, Specific interactions, Spherical beads, Synovial fluid, Thermal diffusivity, Thick slabs, Time variation, Transient diffusion, Ultrapure water, Various species, Water content, Water loss, Water transport, Weight loss, Weight ratio, Weight ratios, Wound healing.
- Teeft :
- Actual alginate concentration, Alginate, Alginate content, Alginate solutions, Alginic acid, Aqueous solution, Average temperature, Average weight, Bead, Beads, Biologie forestiere, Calcium, Calcium chloride medium, Calcium chloride solution, Calcium content, Calcium contents, Calcium diffusion, Calcium figure, Calcium ions, Calcium medium, Characteristic viscosity, Classical problem, Coefficient, Complementary error function, Confidence intervals, Continuous sphere, Dialysis cell, Diffusion coefficient, Diffusion coefficients, Diffusion phenomena, Diffusion rates, Divalent cations, Drug carrier, Experimental data, Experimental procedure, Experimental variations, First hour, Gelation, Gelation mechanism, Gelation time, Generalised model, Good agreement, Guluronic, Guluronic acid, Hardness, Hyaluronate, Hyaluronate content, Hyaluronate loss, Hyaluronate salts, Hyaluronate solutions, Hyaluronic, Hyaluronic acid, Infinite dilution, Instantaneous compliance, John wiley sons, Lower extent, Mechanical properties, Molecular weight, Objective function, Oerther, Physical properties, Polymer, Polysaccharide, Polysaccharide mixtures, Polysaccharidic chains, Preliminary experiments, Pure alginate, Relaxation period, Rheological, Rheological measurements, Rooster comb, Rooster comb hyaluronate, Saturation chamber, Significant loss, Simple model, Small pores, Sodium alginate, Sodium hyaluronate, Specific interactions, Spherical beads, Synovial fluid, Thermal diffusivity, Thick slabs, Time variation, Transient diffusion, Ultrapure water, Various species, Water content, Water loss, Water transport, Weight loss, Weight ratio, Weight ratios, Wound healing.
Abstract
With the aim of producing a biomaterial for surgical applications, the alginate–hyaluronate association has been investigated to combine the gel‐forming properties of alginate with the healing properties of hyaluronate. Gels were prepared by diffusion of calcium into alginate–hyaluronate mixtures, with an alginate content of 20 mg/mL. The hyaluronate source was shown to have significant effect on the aspect and the properties of the gels. The gels have viscoelastic behaviour and the transient measurements carried out in creep mode could be interpreted through a Kelvin–Voigt generalised model: experimental data led to the steady state hardness and a characteristic viscosity of the gel. Gels prepared from Na rooster comb hyaluronate with weight ratio up to 0.50 have satisfactory mechanical properties, and fully stable gels are obtained after a few days; on the contrary, use of lower molecular weight hyaluronate led to loose gels for hyaluronate contents over 0.25. Gel formation was investigated by measurements of the exchange fluxes between the calcium chloride solution and the forming gel, which allowed thorough investigations of the occuring diffusion phenomena of water, calcium ion and hyaluronate. Strong interactions of water with hyaluronate reduce significantly the rate of weight loss from the gel beads and allows higher water content in steady‐state gels. Calcium content in the gel samples could be correlated to the actual alginate concentration, whatever the nature and the weight ratio of hyaluronate. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 206–215, 1999.
Url:
DOI: 10.1002/(SICI)1097-0290(19990420)63:2<206::AID-BIT9>3.0.CO;2-8
Links to Exploration step
ISTEX:1961B86614E032EAA93052A7F32C45B0D748C62DLe document en format XML
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France</mods:affiliation></affiliation></author><author><name sortKey="Le Gall, Herve" sort="Le Gall, Herve" uniqKey="Le Gall H" first="Hervé" last="Le Gall">Hervé Le Gall</name><affiliation><mods:affiliation>Laboratoire des Sciences du Génie Chimique, CNRS–ENSIC–INPL, BP 451, 54001 Nancy, France</mods:affiliation></affiliation></author><author><name sortKey="Payan, Elisabeth" sort="Payan, Elisabeth" uniqKey="Payan E" first="Elisabeth" last="Payan">Elisabeth Payan</name><affiliation><mods:affiliation>Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</mods:affiliation></affiliation></author><author><name sortKey="Lapicque, Francois" sort="Lapicque, Francois" uniqKey="Lapicque F" first="François" last="Lapicque">François Lapicque</name><affiliation><mods:affiliation>E-mail: lapicque@facmed.u‐nancy.fr</mods:affiliation></affiliation><affiliation><mods:affiliation>Laboratoire des Sciences du Génie Chimique, CNRS–ENSIC–INPL, BP 451, 54001 Nancy, France</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: lapicque@facmed.u‐nancy.fr</mods:affiliation></affiliation></author><author><name sortKey="Presle, Nathalie" sort="Presle, Nathalie" uniqKey="Presle N" first="Nathalie" last="Presle">Nathalie Presle</name><affiliation><mods:affiliation>Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</mods:affiliation></affiliation></author><author><name sortKey="Hubert, Patrick" sort="Hubert, Patrick" uniqKey="Hubert P" first="Patrick" last="Hubert">Patrick Hubert</name><affiliation><mods:affiliation>UMR 7568 CNRS–INPL & FR CNRS W0070, ENSIC–INPL, BP 451, 54001 Nancy, France</mods:affiliation></affiliation></author><author><name sortKey="Dexheimer, Jean" sort="Dexheimer, Jean" uniqKey="Dexheimer J" first="Jean" last="Dexheimer">Jean Dexheimer</name><affiliation><mods:affiliation>Laboratoire de Biologie Forestière, Faculté des Sciences, UHP, Nancy 1, BP 239, 54506 Vandoeuvre les Nancy, France</mods:affiliation></affiliation></author><author><name sortKey="Netter, Patrick" sort="Netter, Patrick" uniqKey="Netter P" first="Patrick" last="Netter">Patrick Netter</name><affiliation><mods:affiliation>Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</mods:affiliation></affiliation></author><author><name sortKey="Lapicque, Francoise" sort="Lapicque, Francoise" uniqKey="Lapicque F" first="Françoise" last="Lapicque">Françoise Lapicque</name><affiliation><mods:affiliation>E-mail: lapicque@facmed.u‐nancy.fr</mods:affiliation></affiliation><affiliation><mods:affiliation>Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: lapicque@facmed.u‐nancy.fr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biotechnology and Bioengineering</title><title level="j" type="alt">BIOTECHNOLOGY AND BIOENGINEERING</title><idno type="ISSN">0006-3592</idno><idno type="eISSN">1097-0290</idno><imprint><biblScope unit="vol">63</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="206">206</biblScope><biblScope unit="page" to="215">215</biblScope><biblScope unit="page-count">10</biblScope><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="1999-04-20">1999-04-20</date></imprint><idno type="ISSN">0006-3592</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-3592</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Actual alginate concentration</term><term>Alginate</term><term>Alginate content</term><term>Alginate solutions</term><term>Alginic acid</term><term>Aqueous solution</term><term>Average temperature</term><term>Average weight</term><term>Bead</term><term>Beads</term><term>Biologie forestiere</term><term>Calcium</term><term>Calcium chloride medium</term><term>Calcium chloride solution</term><term>Calcium content</term><term>Calcium contents</term><term>Calcium diffusion</term><term>Calcium figure</term><term>Calcium ions</term><term>Calcium medium</term><term>Characteristic viscosity</term><term>Classical problem</term><term>Coefficient</term><term>Complementary error function</term><term>Confidence intervals</term><term>Continuous sphere</term><term>Dialysis cell</term><term>Diffusion coefficient</term><term>Diffusion coefficients</term><term>Diffusion phenomena</term><term>Diffusion rates</term><term>Divalent cations</term><term>Drug carrier</term><term>Experimental data</term><term>Experimental procedure</term><term>Experimental variations</term><term>First hour</term><term>Gelation</term><term>Gelation mechanism</term><term>Gelation time</term><term>Generalised model</term><term>Good agreement</term><term>Guluronic</term><term>Guluronic acid</term><term>Hardness</term><term>Hyaluronate</term><term>Hyaluronate content</term><term>Hyaluronate loss</term><term>Hyaluronate salts</term><term>Hyaluronate solutions</term><term>Hyaluronic</term><term>Hyaluronic acid</term><term>Infinite dilution</term><term>Instantaneous compliance</term><term>John wiley sons</term><term>Lower extent</term><term>Mechanical properties</term><term>Molecular weight</term><term>Objective function</term><term>Oerther</term><term>Physical properties</term><term>Polymer</term><term>Polysaccharide</term><term>Polysaccharide mixtures</term><term>Polysaccharidic chains</term><term>Preliminary experiments</term><term>Pure alginate</term><term>Relaxation period</term><term>Rheological</term><term>Rheological measurements</term><term>Rooster comb</term><term>Rooster comb hyaluronate</term><term>Saturation chamber</term><term>Significant loss</term><term>Simple model</term><term>Small pores</term><term>Sodium alginate</term><term>Sodium hyaluronate</term><term>Specific interactions</term><term>Spherical beads</term><term>Synovial fluid</term><term>Thermal diffusivity</term><term>Thick slabs</term><term>Time variation</term><term>Transient diffusion</term><term>Ultrapure water</term><term>Various species</term><term>Water content</term><term>Water loss</term><term>Water transport</term><term>Weight loss</term><term>Weight ratio</term><term>Weight ratios</term><term>Wound healing</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actual alginate concentration</term><term>Alginate</term><term>Alginate content</term><term>Alginate solutions</term><term>Alginic acid</term><term>Aqueous solution</term><term>Average temperature</term><term>Average weight</term><term>Bead</term><term>Beads</term><term>Biologie forestiere</term><term>Calcium</term><term>Calcium chloride medium</term><term>Calcium chloride solution</term><term>Calcium content</term><term>Calcium contents</term><term>Calcium diffusion</term><term>Calcium figure</term><term>Calcium ions</term><term>Calcium medium</term><term>Characteristic viscosity</term><term>Classical problem</term><term>Coefficient</term><term>Complementary error function</term><term>Confidence intervals</term><term>Continuous sphere</term><term>Dialysis cell</term><term>Diffusion coefficient</term><term>Diffusion coefficients</term><term>Diffusion phenomena</term><term>Diffusion rates</term><term>Divalent cations</term><term>Drug carrier</term><term>Experimental data</term><term>Experimental procedure</term><term>Experimental variations</term><term>First hour</term><term>Gelation</term><term>Gelation mechanism</term><term>Gelation time</term><term>Generalised model</term><term>Good agreement</term><term>Guluronic</term><term>Guluronic acid</term><term>Hardness</term><term>Hyaluronate</term><term>Hyaluronate content</term><term>Hyaluronate loss</term><term>Hyaluronate salts</term><term>Hyaluronate solutions</term><term>Hyaluronic</term><term>Hyaluronic acid</term><term>Infinite dilution</term><term>Instantaneous compliance</term><term>John wiley sons</term><term>Lower extent</term><term>Mechanical properties</term><term>Molecular weight</term><term>Objective function</term><term>Oerther</term><term>Physical properties</term><term>Polymer</term><term>Polysaccharide</term><term>Polysaccharide mixtures</term><term>Polysaccharidic chains</term><term>Preliminary experiments</term><term>Pure alginate</term><term>Relaxation period</term><term>Rheological</term><term>Rheological measurements</term><term>Rooster comb</term><term>Rooster comb hyaluronate</term><term>Saturation chamber</term><term>Significant loss</term><term>Simple model</term><term>Small pores</term><term>Sodium alginate</term><term>Sodium hyaluronate</term><term>Specific interactions</term><term>Spherical beads</term><term>Synovial fluid</term><term>Thermal diffusivity</term><term>Thick slabs</term><term>Time variation</term><term>Transient diffusion</term><term>Ultrapure water</term><term>Various species</term><term>Water content</term><term>Water loss</term><term>Water transport</term><term>Weight loss</term><term>Weight ratio</term><term>Weight ratios</term><term>Wound healing</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">With the aim of producing a biomaterial for surgical applications, the alginate–hyaluronate association has been investigated to combine the gel‐forming properties of alginate with the healing properties of hyaluronate. Gels were prepared by diffusion of calcium into alginate–hyaluronate mixtures, with an alginate content of 20 mg/mL. The hyaluronate source was shown to have significant effect on the aspect and the properties of the gels. The gels have viscoelastic behaviour and the transient measurements carried out in creep mode could be interpreted through a Kelvin–Voigt generalised model: experimental data led to the steady state hardness and a characteristic viscosity of the gel. Gels prepared from Na rooster comb hyaluronate with weight ratio up to 0.50 have satisfactory mechanical properties, and fully stable gels are obtained after a few days; on the contrary, use of lower molecular weight hyaluronate led to loose gels for hyaluronate contents over 0.25. Gel formation was investigated by measurements of the exchange fluxes between the calcium chloride solution and the forming gel, which allowed thorough investigations of the occuring diffusion phenomena of water, calcium ion and hyaluronate. Strong interactions of water with hyaluronate reduce significantly the rate of weight loss from the gel beads and allows higher water content in steady‐state gels. Calcium content in the gel samples could be correlated to the actual alginate concentration, whatever the nature and the weight ratio of hyaluronate. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 206–215, 1999.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>hyaluronate</json:string><json:string>alginate</json:string><json:string>gelation</json:string><json:string>polysaccharide</json:string><json:string>oerther</json:string><json:string>weight loss</json:string><json:string>calcium content</json:string><json:string>gelation time</json:string><json:string>hyaluronic acid</json:string><json:string>rheological</json:string><json:string>hyaluronic</json:string><json:string>experimental data</json:string><json:string>guluronic</json:string><json:string>calcium chloride solution</json:string><json:string>bead</json:string><json:string>mechanical properties</json:string><json:string>polymer</json:string><json:string>hyaluronate solutions</json:string><json:string>ultrapure water</json:string><json:string>rheological measurements</json:string><json:string>good agreement</json:string><json:string>diffusion coefficient</json:string><json:string>molecular weight</json:string><json:string>calcium ions</json:string><json:string>characteristic viscosity</json:string><json:string>pure alginate</json:string><json:string>divalent cations</json:string><json:string>hyaluronate content</json:string><json:string>diffusion phenomena</json:string><json:string>beads</json:string><json:string>water loss</json:string><json:string>alginic acid</json:string><json:string>generalised model</json:string><json:string>sodium alginate</json:string><json:string>instantaneous compliance</json:string><json:string>synovial fluid</json:string><json:string>diffusion coefficients</json:string><json:string>infinite dilution</json:string><json:string>hardness</json:string><json:string>calcium</json:string><json:string>gelation mechanism</json:string><json:string>significant loss</json:string><json:string>diffusion rates</json:string><json:string>sodium hyaluronate</json:string><json:string>hyaluronate salts</json:string><json:string>rooster comb</json:string><json:string>thick slabs</json:string><json:string>biologie forestiere</json:string><json:string>aqueous solution</json:string><json:string>guluronic acid</json:string><json:string>rooster comb hyaluronate</json:string><json:string>weight ratio</json:string><json:string>weight ratios</json:string><json:string>dialysis cell</json:string><json:string>calcium chloride medium</json:string><json:string>physical properties</json:string><json:string>saturation chamber</json:string><json:string>average weight</json:string><json:string>alginate solutions</json:string><json:string>experimental procedure</json:string><json:string>preliminary experiments</json:string><json:string>calcium contents</json:string><json:string>wound healing</json:string><json:string>water content</json:string><json:string>calcium medium</json:string><json:string>polysaccharidic chains</json:string><json:string>actual alginate concentration</json:string><json:string>small pores</json:string><json:string>calcium figure</json:string><json:string>relaxation period</json:string><json:string>john wiley sons</json:string><json:string>first hour</json:string><json:string>experimental variations</json:string><json:string>hyaluronate loss</json:string><json:string>time variation</json:string><json:string>water transport</json:string><json:string>continuous sphere</json:string><json:string>various species</json:string><json:string>simple model</json:string><json:string>classical problem</json:string><json:string>complementary error function</json:string><json:string>objective function</json:string><json:string>alginate content</json:string><json:string>confidence intervals</json:string><json:string>lower extent</json:string><json:string>drug carrier</json:string><json:string>calcium diffusion</json:string><json:string>polysaccharide mixtures</json:string><json:string>specific interactions</json:string><json:string>thermal diffusivity</json:string><json:string>average temperature</json:string><json:string>transient diffusion</json:string><json:string>spherical beads</json:string><json:string>coefficient</json:string></teeft></keywords><author><json:item><name>Sandra Oerther</name><affiliations><json:string>Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</json:string></affiliations></json:item><json:item><name>Hervé Le Gall</name><affiliations><json:string>Laboratoire des Sciences du Génie Chimique, CNRS–ENSIC–INPL, BP 451, 54001 Nancy, France</json:string></affiliations></json:item><json:item><name>Elisabeth Payan</name><affiliations><json:string>Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</json:string></affiliations></json:item><json:item><name>François Lapicque</name><affiliations><json:string>Laboratoire des Sciences du Génie Chimique, CNRS–ENSIC–INPL, BP 451, 54001 Nancy, France</json:string><json:string>E-mail: lapicque@facmed.u‐nancy.fr</json:string></affiliations></json:item><json:item><name>Nathalie Presle</name><affiliations><json:string>Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</json:string></affiliations></json:item><json:item><name>Patrick Hubert</name><affiliations><json:string>UMR 7568 CNRS–INPL & FR CNRS W0070, ENSIC–INPL, BP 451, 54001 Nancy, France</json:string></affiliations></json:item><json:item><name>Jean Dexheimer</name><affiliations><json:string>Laboratoire de Biologie Forestière, Faculté des Sciences, UHP, Nancy 1, BP 239, 54506 Vandoeuvre les Nancy, France</json:string></affiliations></json:item><json:item><name>Patrick 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Gels were prepared by diffusion of calcium into alginate–hyaluronate mixtures, with an alginate content of 20 mg/mL. The hyaluronate source was shown to have significant effect on the aspect and the properties of the gels. The gels have viscoelastic behaviour and the transient measurements carried out in creep mode could be interpreted through a Kelvin–Voigt generalised model: experimental data led to the steady state hardness and a characteristic viscosity of the gel. Gels prepared from Na rooster comb hyaluronate with weight ratio up to 0.50 have satisfactory mechanical properties, and fully stable gels are obtained after a few days; on the contrary, use of lower molecular weight hyaluronate led to loose gels for hyaluronate contents over 0.25. Gel formation was investigated by measurements of the exchange fluxes between the calcium chloride solution and the forming gel, which allowed thorough investigations of the occuring diffusion phenomena of water, calcium ion and hyaluronate. Strong interactions of water with hyaluronate reduce significantly the rate of weight loss from the gel beads and allows higher water content in steady‐state gels. Calcium content in the gel samples could be correlated to the actual alginate concentration, whatever the nature and the weight ratio of hyaluronate. © 1999 John Wiley & Sons, Inc. 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type="family">Netter</namePart><affiliation>Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</affiliation><affiliation>Correspondence address: Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Françoise</namePart><namePart type="family">Lapicque</namePart><affiliation>E-mail: lapicque@facmed.u‐nancy.fr</affiliation><affiliation>Physiopathologie et Pharmacologie Articulaires, UMR 7561 CNRS–UHP Nancy 1 & FR CNRS W0070, Faculté de Médecine, BP 184, 54505 Vandœuvre les Nancy, France</affiliation><affiliation>E-mail: lapicque@facmed.u‐nancy.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>John Wiley & Sons, Inc.</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">1999-04-20</dateIssued><dateCaptured encoding="w3cdtf">1998-02-27</dateCaptured><dateValid encoding="w3cdtf">1998-10-14</dateValid><copyrightDate encoding="w3cdtf">1999</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">7</extent><extent unit="tables">1</extent><extent unit="references">33</extent><extent unit="words">6169</extent></physicalDescription><abstract lang="en">With the aim of producing a biomaterial for surgical applications, the alginate–hyaluronate association has been investigated to combine the gel‐forming properties of alginate with the healing properties of hyaluronate. Gels were prepared by diffusion of calcium into alginate–hyaluronate mixtures, with an alginate content of 20 mg/mL. The hyaluronate source was shown to have significant effect on the aspect and the properties of the gels. The gels have viscoelastic behaviour and the transient measurements carried out in creep mode could be interpreted through a Kelvin–Voigt generalised model: experimental data led to the steady state hardness and a characteristic viscosity of the gel. Gels prepared from Na rooster comb hyaluronate with weight ratio up to 0.50 have satisfactory mechanical properties, and fully stable gels are obtained after a few days; on the contrary, use of lower molecular weight hyaluronate led to loose gels for hyaluronate contents over 0.25. Gel formation was investigated by measurements of the exchange fluxes between the calcium chloride solution and the forming gel, which allowed thorough investigations of the occuring diffusion phenomena of water, calcium ion and hyaluronate. Strong interactions of water with hyaluronate reduce significantly the rate of weight loss from the gel beads and allows higher water content in steady‐state gels. Calcium content in the gel samples could be correlated to the actual alginate concentration, whatever the nature and the weight ratio of hyaluronate. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 206–215, 1999.</abstract><subject lang="en"><genre>keywords</genre><topic>sodium alginate</topic><topic>sodium hyaluronate</topic><topic>rheological measurements</topic><topic>diffusion phenomena</topic></subject><relatedItem type="host"><titleInfo><title>Biotechnology and Bioengineering</title></titleInfo><titleInfo type="abbreviated"><title>Biotechnol. Bioeng.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0006-3592</identifier><identifier type="eISSN">1097-0290</identifier><identifier type="DOI">10.1002/(ISSN)1097-0290</identifier><identifier type="PublisherID">BIT</identifier><part><date>1999</date><detail type="volume"><caption>vol.</caption><number>63</number></detail><detail type="issue"><caption>no.</caption><number>2</number></detail><extent unit="pages"><start>206</start><end>215</end><total>10</total></extent></part></relatedItem><identifier type="istex">1961B86614E032EAA93052A7F32C45B0D748C62D</identifier><identifier type="ark">ark:/67375/WNG-4XBCGRBN-L</identifier><identifier type="DOI">10.1002/(SICI)1097-0290(19990420)63:2<206::AID-BIT9>3.0.CO;2-8</identifier><identifier type="ArticleID">BIT9</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1999 John Wiley & Sons, Inc.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>John Wiley & Sons, Inc.</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/1961B86614E032EAA93052A7F32C45B0D748C62D/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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