What’s in your buffer? Solute altered millisecond motions detected by solution NMR
Identifieur interne : 000065 ( Pmc/Corpus ); précédent : 000064; suivant : 000066What’s in your buffer? Solute altered millisecond motions detected by solution NMR
Auteurs : Madeline Wong ; Gennady Khirich ; J. Patrick LoriaSource :
- Biochemistry [ 0006-2960 ] ; 2013.
Abstract
To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate, in comparison to MES- and HEPES-buffered references, on the chemical shift perturbation and millisecond, chemical or conformational exchange motions in the enzyme Ribonuclease A (RNase A), Triosephosphate Isomerase (TIM) and HisF. The results indicate that addition of these solutes has a small effect on 1H and 15N chemical shifts for RNase A and TIM but significant effects for HisF. For RNase A and TIM, Carr-Purcell-Meiboom-Gill relaxation dispersion experiments, however, show significant solute-dependent changes in conformational exchange motions. Some residues show loss of millisecond (ms) motions relative to the reference sample upon addition of solute, while others experience an enhancement. Comparison of exchange parameters obtained from fits of dispersion data indicates changes in either or both equilibrium populations and chemical shifts between conformations. Furthermore, the exchange kinetics are altered in many cases. The results demonstrate that common solute molecules can alter observed enzyme ms motions and play a more active role than what is routinely believed.
Url:
DOI: 10.1021/bi400973e
PubMed: 23991940
PubMed Central: 4096712
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PMC:4096712Le document en format XML
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<author><name sortKey="Wong, Madeline" sort="Wong, Madeline" uniqKey="Wong M" first="Madeline" last="Wong">Madeline Wong</name>
<affiliation><nlm:aff id="A1">Department of Chemistry, Yale University, New Haven, CT 06520</nlm:aff>
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<author><name sortKey="Khirich, Gennady" sort="Khirich, Gennady" uniqKey="Khirich G" first="Gennady" last="Khirich">Gennady Khirich</name>
<affiliation><nlm:aff id="A1">Department of Chemistry, Yale University, New Haven, CT 06520</nlm:aff>
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<author><name sortKey="Loria, J Patrick" sort="Loria, J Patrick" uniqKey="Loria J" first="J. Patrick" last="Loria">J. Patrick Loria</name>
<affiliation><nlm:aff id="A1">Department of Chemistry, Yale University, New Haven, CT 06520</nlm:aff>
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<affiliation><nlm:aff id="A2">Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520</nlm:aff>
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<affiliation><nlm:aff id="A1">Department of Chemistry, Yale University, New Haven, CT 06520</nlm:aff>
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<author><name sortKey="Loria, J Patrick" sort="Loria, J Patrick" uniqKey="Loria J" first="J. Patrick" last="Loria">J. Patrick Loria</name>
<affiliation><nlm:aff id="A1">Department of Chemistry, Yale University, New Haven, CT 06520</nlm:aff>
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<affiliation><nlm:aff id="A2">Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520</nlm:aff>
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<series><title level="j">Biochemistry</title>
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<front><div type="abstract" xml:lang="en"><p id="P1">To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate, in comparison to MES- and HEPES-buffered references, on the chemical shift perturbation and millisecond, chemical or conformational exchange motions in the enzyme Ribonuclease A (RNase A), Triosephosphate Isomerase (TIM) and HisF. The results indicate that addition of these solutes has a small effect on <sup>1</sup>
H and <sup>15</sup>
N chemical shifts for RNase A and TIM but significant effects for HisF. For RNase A and TIM, Carr-Purcell-Meiboom-Gill relaxation dispersion experiments, however, show significant solute-dependent changes in conformational exchange motions. Some residues show loss of millisecond (ms) motions relative to the reference sample upon addition of solute, while others experience an enhancement. Comparison of exchange parameters obtained from fits of dispersion data indicates changes in either or both equilibrium populations and chemical shifts between conformations. Furthermore, the exchange kinetics are altered in many cases. The results demonstrate that common solute molecules can alter observed enzyme ms motions and play a more active role than what is routinely believed.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
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<front><journal-meta><journal-id journal-id-type="nlm-journal-id">0370623</journal-id>
<journal-id journal-id-type="pubmed-jr-id">1028</journal-id>
<journal-id journal-id-type="nlm-ta">Biochemistry</journal-id>
<journal-id journal-id-type="iso-abbrev">Biochemistry</journal-id>
<journal-title-group><journal-title>Biochemistry</journal-title>
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<issn pub-type="ppub">0006-2960</issn>
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<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
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<title-group><article-title>What’s in your buffer? Solute altered millisecond motions detected by solution NMR</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Wong</surname>
<given-names>Madeline</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref rid="FN2" ref-type="author-notes">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Khirich</surname>
<given-names>Gennady</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
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<contrib contrib-type="author"><name><surname>Loria</surname>
<given-names>J. Patrick</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="aff" rid="A2">2</xref>
<xref rid="FN1" ref-type="author-notes">*</xref>
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<aff id="A1"><label>1</label>
Department of Chemistry, Yale University, New Haven, CT 06520</aff>
<aff id="A2"><label>2</label>
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520</aff>
<author-notes><corresp id="FN1"><label>*</label>
<bold>Corresponding Author:</bold>
<email>patrick.loria@yale.edu</email>
; phone: 203-436-2518</corresp>
<fn id="FN2" fn-type="present-address"><label>†</label>
<p><bold>Present Addresses:</bold>
Department of Chemistry, Massachusetts Institute of Technology</p>
</fn>
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<pub-date pub-type="nihms-submitted"><day>30</day>
<month>5</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="epub"><day>30</day>
<month>8</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="ppub"><day>17</day>
<month>9</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>17</day>
<month>9</month>
<year>2014</year>
</pub-date>
<volume>52</volume>
<issue>37</issue>
<fpage>6548</fpage>
<lpage>6558</lpage>
<pmc-comment>elocation-id from pubmed: 10.1021/bi400973e</pmc-comment>
<abstract><p id="P1">To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate, in comparison to MES- and HEPES-buffered references, on the chemical shift perturbation and millisecond, chemical or conformational exchange motions in the enzyme Ribonuclease A (RNase A), Triosephosphate Isomerase (TIM) and HisF. The results indicate that addition of these solutes has a small effect on <sup>1</sup>
H and <sup>15</sup>
N chemical shifts for RNase A and TIM but significant effects for HisF. For RNase A and TIM, Carr-Purcell-Meiboom-Gill relaxation dispersion experiments, however, show significant solute-dependent changes in conformational exchange motions. Some residues show loss of millisecond (ms) motions relative to the reference sample upon addition of solute, while others experience an enhancement. Comparison of exchange parameters obtained from fits of dispersion data indicates changes in either or both equilibrium populations and chemical shifts between conformations. Furthermore, the exchange kinetics are altered in many cases. The results demonstrate that common solute molecules can alter observed enzyme ms motions and play a more active role than what is routinely believed.</p>
</abstract>
<kwd-group><kwd>Solution NMR</kwd>
<kwd>Conformational motions</kwd>
<kwd>Buffer effects</kwd>
<kwd>Solute effects</kwd>
<kwd>Enzymes</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
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