Crystallization and characterization of the thallium form of the Oxytricha nova G-quadruplex
Identifieur interne : 000032 ( Ncbi/Merge ); précédent : 000031; suivant : 000033Crystallization and characterization of the thallium form of the Oxytricha nova G-quadruplex
Auteurs : Michelle L. Gill [États-Unis] ; Scott A. Strobel [États-Unis] ; J. Patrick Loria [États-Unis]Source :
- Nucleic Acids Research [ 0305-1048 ] ; 2006.
Abstract
The crystal structure of the Tl+ form of the G-quadruplex formed from the
Url:
DOI: 10.1093/nar/gkl616
PubMed: 16945956
PubMed Central: 1636370
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Strobel</name><affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Molecular Biophysics and Biochemistry, Yale University</institution><addr-line>New Haven, CT 06520, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>New Haven, CT 06520</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="au2"><institution>Department of Chemistry, Yale University</institution><addr-line>New Haven, CT 06520, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>New Haven, CT 06520</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Loria, J Patrick" sort="Loria, J Patrick" uniqKey="Loria J" first="J. Patrick" last="Loria">J. Patrick Loria</name><affiliation wicri:level="2"><nlm:aff id="au2"><institution>Department of Chemistry, Yale University</institution><addr-line>New Haven, CT 06520, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>New Haven, CT 06520</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16945956</idno><idno type="pmc">1636370</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636370</idno><idno type="RBID">PMC:1636370</idno><idno type="doi">10.1093/nar/gkl616</idno><date when="2006">2006</date><idno type="wicri:Area/Pmc/Corpus">000083</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000083</idno><idno type="wicri:Area/Pmc/Curation">000083</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000083</idno><idno type="wicri:Area/Pmc/Checkpoint">000094</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000094</idno><idno type="wicri:Area/Ncbi/Merge">000032</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Crystallization and characterization of the thallium form of the <italic>Oxytricha nova</italic> G-quadruplex</title><author><name sortKey="Gill, Michelle L" sort="Gill, Michelle L" uniqKey="Gill M" first="Michelle L." last="Gill">Michelle L. Gill</name><affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Molecular Biophysics and Biochemistry, Yale University</institution><addr-line>New Haven, CT 06520, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>New Haven, CT 06520</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Strobel, Scott A" sort="Strobel, Scott A" uniqKey="Strobel S" first="Scott A." last="Strobel">Scott A. Strobel</name><affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Molecular Biophysics and Biochemistry, Yale University</institution><addr-line>New Haven, CT 06520, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>New Haven, CT 06520</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="au2"><institution>Department of Chemistry, Yale University</institution><addr-line>New Haven, CT 06520, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>New Haven, CT 06520</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Loria, J Patrick" sort="Loria, J Patrick" uniqKey="Loria J" first="J. Patrick" last="Loria">J. Patrick Loria</name><affiliation wicri:level="2"><nlm:aff id="au2"><institution>Department of Chemistry, Yale University</institution><addr-line>New Haven, CT 06520, USA</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>New Haven, CT 06520</wicri:regionArea><placeName><region type="state">Connecticut</region></placeName></affiliation></author></analytic><series><title level="j">Nucleic Acids Research</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The crystal structure of the Tl<sup>+</sup> form of the G-quadruplex formed from the <italic>Oxytricha nova</italic> telomere sequence, d(G<sub>4</sub>T<sub>4</sub>G<sub>4</sub>), has been solved to 1.55 Å. This G-quadruplex contains five Tl<sup>+</sup> ions, three of which are interspersed between adjacent G-quartet planes and one in each of the two thymine loops. The structure displays a high degree of similarity to the K<sup>+</sup> crystal structure [Haider <italic>et al.</italic> (2002), <italic>J. Mol. Biol.</italic>, 320, 189–200], including the number and location of the monovalent cation binding sites. The highly isomorphic nature of the two structures, which contain such a large number of monovalent binding sites (relative to nucleic acid content), verifies the ability of Tl<sup>+</sup> to mimic K<sup>+</sup> in nucleic acids. Information from this report confirms and extends the assignment of <sup>205</sup>Tl resonances from a previous report [Gill <italic>et al.</italic> (2005), <italic>J. Am. Chem. Soc.</italic>, 127, 16 723–16 732] where <sup>205</sup>Tl NMR was used to study monovalent cation binding to this G-quadruplex. The assignment of these resonances provides evidence for the occurrence of conformational dynamics in the thymine loop region that is in slow exchange on the <sup>205</sup>Tl timescale.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Nucleic Acids Res</journal-id><journal-id journal-id-type="publisher-id">Nucleic Acids Research</journal-id><journal-title>Nucleic Acids Research</journal-title><issn pub-type="ppub">0305-1048</issn><issn pub-type="epub">1362-4962</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16945956</article-id><article-id pub-id-type="pmc">1636370</article-id><article-id pub-id-type="doi">10.1093/nar/gkl616</article-id><article-categories><subj-group subj-group-type="heading"><subject>Structural Biology</subject></subj-group></article-categories><title-group><article-title>Crystallization and characterization of the thallium form of the <italic>Oxytricha nova</italic> G-quadruplex</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Gill</surname><given-names>Michelle L.</given-names></name><xref rid="au1" ref-type="aff">1</xref></contrib><contrib contrib-type="author"><name><surname>Strobel</surname><given-names>Scott A.</given-names></name><xref rid="au1" ref-type="aff">1</xref><xref rid="au2" ref-type="aff">2</xref><xref ref-type="corresp" rid="cor2">*</xref></contrib><contrib contrib-type="author"><name><surname>Loria</surname><given-names>J. Patrick</given-names></name><xref rid="au2" ref-type="aff">2</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><aff id="au1"><sup>1</sup><institution>Department of Molecular Biophysics and Biochemistry, Yale University</institution><addr-line>New Haven, CT 06520, USA</addr-line></aff><aff id="au2"><sup>2</sup><institution>Department of Chemistry, Yale University</institution><addr-line>New Haven, CT 06520, USA</addr-line></aff></contrib-group><author-notes><corresp id="cor1"><sup>*</sup>To whom correspondence should be addressed. Tel: +1 203 436 4847; Fax: +1 203 432 6144; Email: <email>patrick.loria@yale.edu</email></corresp><corresp id="cor2"><sup>*</sup>Correspondence may also be addressed to Scott A. Strobel. Tel: +1 203 432 9772; Fax: +1 203 432 5767; Email: <email>scott.strobel@yale.edu</email></corresp></author-notes><pmc-comment>For NAR: both ppub and collection dates generated for PMC processing 1/27/05 beck</pmc-comment>
<pub-date pub-type="collection"><month>9</month><year>2006</year></pub-date><pub-date pub-type="ppub"><month>9</month><year>2006</year></pub-date><pub-date pub-type="epub"><day>31</day><month>8</month><year>2006</year></pub-date><volume>34</volume><issue>16</issue><fpage>4506</fpage><lpage>4514</lpage><history><date date-type="received"><day>20</day><month>6</month><year>2006</year></date><date date-type="rev-recd"><day>04</day><month>8</month><year>2006</year></date><date date-type="accepted"><day>04</day><month>8</month><year>2006</year></date></history><copyright-statement>© 2006 The Author(s)</copyright-statement><copyright-year>2006</copyright-year><license license-type="openaccess"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/uk/"></ext-link>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</p></license><abstract><p>The crystal structure of the Tl<sup>+</sup> form of the G-quadruplex formed from the <italic>Oxytricha nova</italic> telomere sequence, d(G<sub>4</sub>T<sub>4</sub>G<sub>4</sub>), has been solved to 1.55 Å. This G-quadruplex contains five Tl<sup>+</sup> ions, three of which are interspersed between adjacent G-quartet planes and one in each of the two thymine loops. The structure displays a high degree of similarity to the K<sup>+</sup> crystal structure [Haider <italic>et al.</italic> (2002), <italic>J. Mol. Biol.</italic>, 320, 189–200], including the number and location of the monovalent cation binding sites. The highly isomorphic nature of the two structures, which contain such a large number of monovalent binding sites (relative to nucleic acid content), verifies the ability of Tl<sup>+</sup> to mimic K<sup>+</sup> in nucleic acids. Information from this report confirms and extends the assignment of <sup>205</sup>Tl resonances from a previous report [Gill <italic>et al.</italic> (2005), <italic>J. Am. Chem. Soc.</italic>, 127, 16 723–16 732] where <sup>205</sup>Tl NMR was used to study monovalent cation binding to this G-quadruplex. The assignment of these resonances provides evidence for the occurrence of conformational dynamics in the thymine loop region that is in slow exchange on the <sup>205</sup>Tl timescale.</p></abstract></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country><region><li>Connecticut</li></region></list><tree><country name="États-Unis"><region name="Connecticut"><name sortKey="Gill, Michelle L" sort="Gill, Michelle L" uniqKey="Gill M" first="Michelle L." last="Gill">Michelle L. Gill</name></region><name sortKey="Loria, J Patrick" sort="Loria, J Patrick" uniqKey="Loria J" first="J. Patrick" last="Loria">J. Patrick Loria</name><name sortKey="Strobel, Scott A" sort="Strobel, Scott A" uniqKey="Strobel S" first="Scott A." last="Strobel">Scott A. Strobel</name><name sortKey="Strobel, Scott A" sort="Strobel, Scott A" uniqKey="Strobel S" first="Scott A." last="Strobel">Scott A. Strobel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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