Evaluation of virus neutralization antibody levels in diabetic mutant mice
Identifieur interne : 003297 ( Istex/Corpus ); précédent : 003296; suivant : 003298Evaluation of virus neutralization antibody levels in diabetic mutant mice
Auteurs : Roger M. Loria ; Louise B. Montgomery ; Nancy Tuttle-Fuller ; M. S. Hall ; M. Gregg ; Vernon M. ChinchilliSource :
- Journal of Virological Methods [ 0166-0934 ] ; 1990.
English descriptors
- Teeft :
- Academic press, Actual plaque reduction, Animal viruses, Antibody levels, Clinical diabetes, Coxsackievirus, Diabetes, Diabetes mellitus, Diabetic, Diagnostic procedures, Different groups, Dilution, Endpoint procedure, Food intake, Fundamental techniques, Genetic predisposition, Health association, Heterozygous, Heterozygous mice, Homozygous mice, Identical genotype, Input dose, Loria, Misty coat color, Multivariate, Multivariate analysis, Multivariate procedure, Multivariate wilcoxon rank, Mutant, Mutant mice, Neutralization, Neutralization activity, Neutralization antibodies, Neutralization antibody, Neutralization antibody levels, Neutralization levels, Overt diabetes, Plaque, Plaque reduction, Plaque reduction assay, Point procedure, Present report, Qualitative information, Quantitative information, Serum dilution, Serum dilutions, Serum neutralization levels, Significant differences, Statistical analysis, Test results, Test statistics, Virus plaque reduction.
Abstract
Abstract: Experiments were designed to determine whether genetic predisposition to diabetes or overt diabetes in the identical genotype had an effect on the level of neutralization antibodies to coxsackievirus B4 (CB4). Quantitation of neutralization antibody (NT) levels against CB4 was performed using both the 50% endpoint procedure and the multivariate Wilcoxon rank sum test. The results of these experiments demonstrate that the use of the multivariate procedure for the analysis of neutralization antibody levels provides both quantitative and qualitative information not evident when only the classical 50% end point procedure is used. Moreover, when comparison on antibody levels between different groups is being considered, the power of the comparison is markedly greater using the multivariate Wilcoxon rank sum test results. The present report provide an illustration of the difference in the qualitative and quantitative information obtained by the end-point procedure and the more comprehensive multivariate procedure for the analysis of neutralization antibody levels in diabetic mutant mice infected with coxsackievirus B4.
Url:
DOI: 10.1016/0166-0934(90)90117-X
Links to Exploration step
ISTEX:D5C1C168378B2E3B54EA95C4344FA291D145B48FLe document en format XML
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Loria</name><affiliation><mods:affiliation>Department of Pathology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Montgomery, Louise B" sort="Montgomery, Louise B" uniqKey="Montgomery L" first="Louise B." last="Montgomery">Louise B. Montgomery</name><affiliation><mods:affiliation>Department of Microbiology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Tuttle Fuller, Nancy" sort="Tuttle Fuller, Nancy" uniqKey="Tuttle Fuller N" first="Nancy" last="Tuttle-Fuller">Nancy Tuttle-Fuller</name><affiliation><mods:affiliation>Department of Immunology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Hall, M S" sort="Hall, M S" uniqKey="Hall M" first="M. S." last="Hall">M. S. Hall</name><affiliation><mods:affiliation>Department of Microbiology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Gregg, M" sort="Gregg, M" uniqKey="Gregg M" first="M." last="Gregg">M. Gregg</name><affiliation><mods:affiliation>Department of Biostatistics School of Basic Sciences and Medicine, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Chinchilli, Vernon M" sort="Chinchilli, Vernon M" uniqKey="Chinchilli V" first="Vernon M." last="Chinchilli">Vernon M. Chinchilli</name><affiliation><mods:affiliation>Department of Biostatistics School of Basic Sciences and Medicine, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="ISSN">0166-0934</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990">1990</date><biblScope unit="volume">28</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="235">235</biblScope><biblScope unit="page" to="244">244</biblScope></imprint><idno type="ISSN">0166-0934</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0166-0934</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Academic press</term><term>Actual plaque reduction</term><term>Animal viruses</term><term>Antibody levels</term><term>Clinical diabetes</term><term>Coxsackievirus</term><term>Diabetes</term><term>Diabetes mellitus</term><term>Diabetic</term><term>Diagnostic procedures</term><term>Different groups</term><term>Dilution</term><term>Endpoint procedure</term><term>Food intake</term><term>Fundamental techniques</term><term>Genetic predisposition</term><term>Health association</term><term>Heterozygous</term><term>Heterozygous mice</term><term>Homozygous mice</term><term>Identical genotype</term><term>Input dose</term><term>Loria</term><term>Misty coat color</term><term>Multivariate</term><term>Multivariate analysis</term><term>Multivariate procedure</term><term>Multivariate wilcoxon rank</term><term>Mutant</term><term>Mutant mice</term><term>Neutralization</term><term>Neutralization activity</term><term>Neutralization antibodies</term><term>Neutralization antibody</term><term>Neutralization antibody levels</term><term>Neutralization levels</term><term>Overt diabetes</term><term>Plaque</term><term>Plaque reduction</term><term>Plaque reduction assay</term><term>Point procedure</term><term>Present report</term><term>Qualitative information</term><term>Quantitative information</term><term>Serum dilution</term><term>Serum dilutions</term><term>Serum neutralization levels</term><term>Significant differences</term><term>Statistical analysis</term><term>Test results</term><term>Test statistics</term><term>Virus plaque reduction</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Experiments were designed to determine whether genetic predisposition to diabetes or overt diabetes in the identical genotype had an effect on the level of neutralization antibodies to coxsackievirus B4 (CB4). Quantitation of neutralization antibody (NT) levels against CB4 was performed using both the 50% endpoint procedure and the multivariate Wilcoxon rank sum test. The results of these experiments demonstrate that the use of the multivariate procedure for the analysis of neutralization antibody levels provides both quantitative and qualitative information not evident when only the classical 50% end point procedure is used. Moreover, when comparison on antibody levels between different groups is being considered, the power of the comparison is markedly greater using the multivariate Wilcoxon rank sum test results. The present report provide an illustration of the difference in the qualitative and quantitative information obtained by the end-point procedure and the more comprehensive multivariate procedure for the analysis of neutralization antibody levels in diabetic mutant mice infected with coxsackievirus B4.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>plaque reduction</json:string><json:string>multivariate</json:string><json:string>loria</json:string><json:string>coxsackievirus</json:string><json:string>serum dilution</json:string><json:string>overt diabetes</json:string><json:string>plaque</json:string><json:string>neutralization</json:string><json:string>mutant</json:string><json:string>neutralization antibody levels</json:string><json:string>serum dilutions</json:string><json:string>multivariate wilcoxon rank</json:string><json:string>genetic predisposition</json:string><json:string>different groups</json:string><json:string>multivariate procedure</json:string><json:string>clinical diabetes</json:string><json:string>food intake</json:string><json:string>antibody levels</json:string><json:string>statistical analysis</json:string><json:string>diabetes</json:string><json:string>neutralization antibodies</json:string><json:string>neutralization levels</json:string><json:string>academic press</json:string><json:string>actual plaque reduction</json:string><json:string>point procedure</json:string><json:string>misty coat color</json:string><json:string>homozygous mice</json:string><json:string>heterozygous</json:string><json:string>dilution</json:string><json:string>input dose</json:string><json:string>neutralization antibody</json:string><json:string>identical genotype</json:string><json:string>test statistics</json:string><json:string>virus plaque reduction</json:string><json:string>serum neutralization levels</json:string><json:string>endpoint procedure</json:string><json:string>test results</json:string><json:string>significant differences</json:string><json:string>mutant mice</json:string><json:string>diagnostic procedures</json:string><json:string>present report</json:string><json:string>heterozygous mice</json:string><json:string>neutralization activity</json:string><json:string>plaque reduction assay</json:string><json:string>quantitative information</json:string><json:string>diabetes mellitus</json:string><json:string>multivariate analysis</json:string><json:string>fundamental techniques</json:string><json:string>qualitative information</json:string><json:string>animal viruses</json:string><json:string>health association</json:string><json:string>diabetic</json:string></teeft></keywords><author><json:item><name>Roger M. Loria</name><affiliations><json:string>Department of Pathology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</json:string></affiliations></json:item><json:item><name>Louise B. Montgomery</name><affiliations><json:string>Department of Microbiology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</json:string></affiliations></json:item><json:item><name>Nancy Tuttle-Fuller</name><affiliations><json:string>Department of Immunology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</json:string></affiliations></json:item><json:item><name>M.S. Hall</name><affiliations><json:string>Department of Microbiology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</json:string></affiliations></json:item><json:item><name>M. Gregg</name><affiliations><json:string>Department of Biostatistics School of Basic Sciences and Medicine, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</json:string></affiliations></json:item><json:item><name>Vernon M. Chinchilli</name><affiliations><json:string>Department of Biostatistics School of Basic Sciences and Medicine, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Diabetes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Mutation</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Antibody</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Coxsackievirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>End-point analysis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Multivariate analysis</value></json:item></subject><arkIstex>ark:/67375/6H6-TP4S9WSM-7</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Experiments were designed to determine whether genetic predisposition to diabetes or overt diabetes in the identical genotype had an effect on the level of neutralization antibodies to coxsackievirus B4 (CB4). Quantitation of neutralization antibody (NT) levels against CB4 was performed using both the 50% endpoint procedure and the multivariate Wilcoxon rank sum test. The results of these experiments demonstrate that the use of the multivariate procedure for the analysis of neutralization antibody levels provides both quantitative and qualitative information not evident when only the classical 50% end point procedure is used. Moreover, when comparison on antibody levels between different groups is being considered, the power of the comparison is markedly greater using the multivariate Wilcoxon rank sum test results. The present report provide an illustration of the difference in the qualitative and quantitative information obtained by the end-point procedure and the more comprehensive multivariate procedure for the analysis of neutralization antibody levels in diabetic mutant mice infected with coxsackievirus B4.</abstract><qualityIndicators><score>7.052</score><pdfWordCount>3120</pdfWordCount><pdfCharCount>19641</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>432 x 648 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>161</abstractWordCount><abstractCharCount>1140</abstractCharCount><keywordCount>6</keywordCount></qualityIndicators><title>Evaluation of virus neutralization antibody levels in diabetic mutant mice</title><pmid><json:string>2166746</json:string></pmid><pii><json:string>0166-0934(90)90117-X</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. 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Chinchilli</json:string><json:string>M. Gregg</json:string><json:string>Louise B. Montgomery</json:string><json:string>M. Loria</json:string><json:string>Vernon Nancy</json:string></persName><placeName><json:string>Richmond</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Chick and Like, 1971</json:string><json:string>Loria, 1984</json:string><json:string>Lennette and Schmidt, 1979</json:string><json:string>Dulbecco et al., 1956</json:string><json:string>Wyse and Dulin, 1970</json:string><json:string>Montgomery, 1986</json:string><json:string>Burch et al. (1971)</json:string><json:string>Habel, 1969</json:string><json:string>Lehmann, 1975</json:string><json:string>Montgomery et al., 1988</json:string><json:string>Loria and Montgomery, 1986</json:string><json:string>Lennette (1956)</json:string><json:string>Dulbecco et al. (1956)</json:string><json:string>Fenner et al., 1974</json:string><json:string>Cook et al., 1982</json:string><json:string>Loria, 1986</json:string><json:string>Jordan, 1972</json:string><json:string>Reed and Munch (1938)</json:string><json:string>Montgomery, 1986, 1989</json:string><json:string>Kibrick and Benirschke (1958)</json:string><json:string>Loria et al., 1984</json:string><json:string>Dulbecco, 1952</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-TP4S9WSM-7</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string><json:string>2 - biotechnology & applied microbiology</json:string><json:string>2 - biochemical research methods</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1990</publicationDate><copyrightDate>1990</copyrightDate><doi><json:string>10.1016/0166-0934(90)90117-X</json:string></doi><id>D5C1C168378B2E3B54EA95C4344FA291D145B48F</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-TP4S9WSM-7/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-TP4S9WSM-7/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-TP4S9WSM-7/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Evaluation of virus neutralization antibody levels in diabetic mutant mice</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1990</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Evaluation of virus neutralization antibody levels in diabetic mutant mice</title><author xml:id="author-0000"><persName><forename type="first">Roger M.</forename><surname>Loria</surname></persName><note type="correspondence"><p>Correspondence to: R.M. Loria, Department of Microbiology and Immunology, Box 678, Medical College of Virginia, Richmond VA 23298, U.S.A.</p></note><affiliation>Department of Pathology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Louise B.</forename><surname>Montgomery</surname></persName><affiliation>Department of Microbiology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Nancy</forename><surname>Tuttle-Fuller</surname></persName><affiliation>Department of Immunology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation></author><author xml:id="author-0003"><persName><forename type="first">M.S.</forename><surname>Hall</surname></persName><affiliation>Department of Microbiology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation></author><author xml:id="author-0004"><persName><forename type="first">M.</forename><surname>Gregg</surname></persName><affiliation>Department of Biostatistics School of Basic Sciences and Medicine, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Vernon M.</forename><surname>Chinchilli</surname></persName><affiliation>Department of Biostatistics School of Basic Sciences and Medicine, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation></author><idno type="istex">D5C1C168378B2E3B54EA95C4344FA291D145B48F</idno><idno type="ark">ark:/67375/6H6-TP4S9WSM-7</idno><idno type="DOI">10.1016/0166-0934(90)90117-X</idno><idno type="PII">0166-0934(90)90117-X</idno></analytic><monogr><title level="j">Journal of Virological Methods</title><title level="j" type="abbrev">VIRMET</title><idno type="pISSN">0166-0934</idno><idno type="PII">S0166-0934(00)X0155-3</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990"></date><biblScope unit="volume">28</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="235">235</biblScope><biblScope unit="page" to="244">244</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1990</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Experiments were designed to determine whether genetic predisposition to diabetes or overt diabetes in the identical genotype had an effect on the level of neutralization antibodies to coxsackievirus B4 (CB4). Quantitation of neutralization antibody (NT) levels against CB4 was performed using both the 50% endpoint procedure and the multivariate Wilcoxon rank sum test. The results of these experiments demonstrate that the use of the multivariate procedure for the analysis of neutralization antibody levels provides both quantitative and qualitative information not evident when only the classical 50% end point procedure is used. Moreover, when comparison on antibody levels between different groups is being considered, the power of the comparison is markedly greater using the multivariate Wilcoxon rank sum test results. The present report provide an illustration of the difference in the qualitative and quantitative information obtained by the end-point procedure and the more comprehensive multivariate procedure for the analysis of neutralization antibody levels in diabetic mutant mice infected with coxsackievirus B4.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Diabetes</term></item><item><term>Mutation</term></item><item><term>Antibody</term></item><item><term>Coxsackievirus</term></item><item><term>End-point analysis</term></item><item><term>Multivariate analysis</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1990">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-TP4S9WSM-7/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VIRMET</jid><aid>9090117X</aid><ce:pii>0166-0934(90)90117-X</ce:pii><ce:doi>10.1016/0166-0934(90)90117-X</ce:doi><ce:copyright type="unknown" year="1990"></ce:copyright></item-info><head><ce:title>Evaluation of virus neutralization antibody levels in diabetic mutant mice</ce:title><ce:author-group><ce:author><ce:given-name>Roger M.</ce:given-name><ce:surname>Loria</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Louise B.</ce:given-name><ce:surname>Montgomery</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Nancy</ce:given-name><ce:surname>Tuttle-Fuller</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.S.</ce:given-name><ce:surname>Hall</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Gregg</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>4</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Vernon M.</ce:given-name><ce:surname>Chinchilli</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>4</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>1</ce:label><ce:textfn>Department of Microbiology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>2</ce:label><ce:textfn>Department of Immunology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>3</ce:label><ce:textfn>Department of Pathology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>4</ce:label><ce:textfn>Department of Biostatistics School of Basic Sciences and Medicine, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: R.M. Loria, Department of Microbiology and Immunology, Box 678, Medical College of Virginia, Richmond VA 23298, U.S.A.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="9" month="2" year="1990"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Experiments were designed to determine whether genetic predisposition to diabetes or overt diabetes in the identical genotype had an effect on the level of neutralization antibodies to coxsackievirus B4 (CB4). Quantitation of neutralization antibody (NT) levels against CB4 was performed using both the 50% endpoint procedure and the multivariate Wilcoxon rank sum test. The results of these experiments demonstrate that the use of the multivariate procedure for the analysis of neutralization antibody levels provides both quantitative and qualitative information not evident when only the classical 50% end point procedure is used. Moreover, when comparison on antibody levels between different groups is being considered, the power of the comparison is markedly greater using the multivariate Wilcoxon rank sum test results. The present report provide an illustration of the difference in the qualitative and quantitative information obtained by the end-point procedure and the more comprehensive multivariate procedure for the analysis of neutralization antibody levels in diabetic mutant mice infected with coxsackievirus B4.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Diabetes</ce:text></ce:keyword><ce:keyword><ce:text>Mutation</ce:text></ce:keyword><ce:keyword><ce:text>Antibody</ce:text></ce:keyword><ce:keyword><ce:text>Coxsackievirus</ce:text></ce:keyword><ce:keyword><ce:text>End-point analysis</ce:text></ce:keyword><ce:keyword><ce:text>Multivariate analysis</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Evaluation of virus neutralization antibody levels in diabetic mutant mice</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Evaluation of virus neutralization antibody levels in diabetic mutant mice</title></titleInfo><name type="personal"><namePart type="given">Roger M.</namePart><namePart type="family">Loria</namePart><affiliation>Department of Pathology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation><description>Correspondence to: R.M. Loria, Department of Microbiology and Immunology, Box 678, Medical College of Virginia, Richmond VA 23298, U.S.A.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Louise B.</namePart><namePart type="family">Montgomery</namePart><affiliation>Department of Microbiology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nancy</namePart><namePart type="family">Tuttle-Fuller</namePart><affiliation>Department of Immunology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.S.</namePart><namePart type="family">Hall</namePart><affiliation>Department of Microbiology, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Gregg</namePart><affiliation>Department of Biostatistics School of Basic Sciences and Medicine, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Vernon M.</namePart><namePart type="family">Chinchilli</namePart><affiliation>Department of Biostatistics School of Basic Sciences and Medicine, Virginia Commonwealth University, Richmond, Virginia, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued><copyrightDate encoding="w3cdtf">1990</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Experiments were designed to determine whether genetic predisposition to diabetes or overt diabetes in the identical genotype had an effect on the level of neutralization antibodies to coxsackievirus B4 (CB4). Quantitation of neutralization antibody (NT) levels against CB4 was performed using both the 50% endpoint procedure and the multivariate Wilcoxon rank sum test. The results of these experiments demonstrate that the use of the multivariate procedure for the analysis of neutralization antibody levels provides both quantitative and qualitative information not evident when only the classical 50% end point procedure is used. Moreover, when comparison on antibody levels between different groups is being considered, the power of the comparison is markedly greater using the multivariate Wilcoxon rank sum test results. The present report provide an illustration of the difference in the qualitative and quantitative information obtained by the end-point procedure and the more comprehensive multivariate procedure for the analysis of neutralization antibody levels in diabetic mutant mice infected with coxsackievirus B4.</abstract><subject><genre>Keywords</genre><topic>Diabetes</topic><topic>Mutation</topic><topic>Antibody</topic><topic>Coxsackievirus</topic><topic>End-point analysis</topic><topic>Multivariate analysis</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Virological Methods</title></titleInfo><titleInfo type="abbreviated"><title>VIRMET</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued></originInfo><identifier type="ISSN">0166-0934</identifier><identifier type="PII">S0166-0934(00)X0155-3</identifier><part><date>1990</date><detail type="volume"><number>28</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue-pages"><start>219</start><end>336</end></extent><extent unit="pages"><start>235</start><end>244</end></extent></part></relatedItem><identifier type="istex">D5C1C168378B2E3B54EA95C4344FA291D145B48F</identifier><identifier type="ark">ark:/67375/6H6-TP4S9WSM-7</identifier><identifier type="DOI">10.1016/0166-0934(90)90117-X</identifier><identifier type="PII">0166-0934(90)90117-X</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-TP4S9WSM-7/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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