Firing and intrinsic properties of antennal lobe neurons in the Noctuid moth Agrotis ipsilon.
Identifieur interne : 000049 ( PubMed/Corpus ); précédent : 000048; suivant : 000050Firing and intrinsic properties of antennal lobe neurons in the Noctuid moth Agrotis ipsilon.
Auteurs : Céline Lavialle-Defaix ; Vincent Jacob ; Christelle Monsempès ; Sylvia Anton ; Jean-Pierre Rospars ; Dominique Martinez ; Philippe LucasSource :
- Bio Systems [ 1872-8324 ] ; 2015.
Abstract
The antennal lobe (AL) of the Noctuid moth Agrotis ipsilon has emerged as an excellent model for studying olfactory processing and its plasticity in the central nervous system. Odor-evoked responses of AL neurons and input-to-output transformations involved in pheromone processing are well characterized in this species. However, the intrinsic electrical properties responsible of the firing of AL neurons are poorly known. To this end, patch-clamp recordings in current- and voltage-clamp mode from neurons located in the two main clusters of cell bodies in the ALs were combined with intracellular staining on A. ipsilon males. Staining indicated that the lateral cluster (LC) is composed of 85% of local neurons (LNs) and 15% of projection neurons (PNs). The medial cluster (MC) contains only PNs. Action potentials were readily recorded from the soma in LNs and PNs located in the LC but not from PNs in the MC where recordings showed small or no action potentials. In the LC, the spontaneous activity of about 20% of the LNs presented irregular bursts while being more regular in PNs. We also identified a small population of LNs lacking voltage-gated Na(+) currents and generating spikelets. We focused on the firing properties of LNs since in about 60% of LNs, but not in PNs, action potentials were followed by depolarizing afterpotentials (DAPs). These DAPs could generate a second action potential, so that the activity was composed of action potential doublets. DAPs depended on voltage, Ca(2+)-channels and possibly on Ca(2+)-activated non-specific cationic channels. During steady state current injection, DAPs occurred after each action potential and did not require high-frequency firing. The amplitude of DAPs increased when the interspike interval was small, typically within bursts, likely arising from a Ca(2+) build up. DAPs were more often found in bursting than in non-bursting LNs but do not support bursting activity. DAPs and spike doublets also occurred during odor-evoked activity suggesting that they can mediate olfactory integration in the AL.
DOI: 10.1016/j.biosystems.2015.06.005
PubMed: 26126723
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Electronic address: philippe.lucas@versailles.inra.fr.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Bio Systems</title><idno type="eISSN">1872-8324</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The antennal lobe (AL) of the Noctuid moth Agrotis ipsilon has emerged as an excellent model for studying olfactory processing and its plasticity in the central nervous system. Odor-evoked responses of AL neurons and input-to-output transformations involved in pheromone processing are well characterized in this species. However, the intrinsic electrical properties responsible of the firing of AL neurons are poorly known. To this end, patch-clamp recordings in current- and voltage-clamp mode from neurons located in the two main clusters of cell bodies in the ALs were combined with intracellular staining on A. ipsilon males. Staining indicated that the lateral cluster (LC) is composed of 85% of local neurons (LNs) and 15% of projection neurons (PNs). The medial cluster (MC) contains only PNs. Action potentials were readily recorded from the soma in LNs and PNs located in the LC but not from PNs in the MC where recordings showed small or no action potentials. In the LC, the spontaneous activity of about 20% of the LNs presented irregular bursts while being more regular in PNs. We also identified a small population of LNs lacking voltage-gated Na(+) currents and generating spikelets. We focused on the firing properties of LNs since in about 60% of LNs, but not in PNs, action potentials were followed by depolarizing afterpotentials (DAPs). These DAPs could generate a second action potential, so that the activity was composed of action potential doublets. DAPs depended on voltage, Ca(2+)-channels and possibly on Ca(2+)-activated non-specific cationic channels. During steady state current injection, DAPs occurred after each action potential and did not require high-frequency firing. The amplitude of DAPs increased when the interspike interval was small, typically within bursts, likely arising from a Ca(2+) build up. DAPs were more often found in bursting than in non-bursting LNs but do not support bursting activity. DAPs and spike doublets also occurred during odor-evoked activity suggesting that they can mediate olfactory integration in the AL.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">26126723</PMID><DateCreated><Year>2015</Year><Month>11</Month><Day>30</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-8324</ISSN><JournalIssue CitedMedium="Internet"><Volume>136</Volume><PubDate><Year>2015</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Bio Systems</Title><ISOAbbreviation>BioSystems</ISOAbbreviation></Journal><ArticleTitle>Firing and intrinsic properties of antennal lobe neurons in the Noctuid moth Agrotis ipsilon.</ArticleTitle><Pagination><MedlinePgn>46-58</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biosystems.2015.06.005</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0303-2647(15)00088-X</ELocationID><Abstract><AbstractText>The antennal lobe (AL) of the Noctuid moth Agrotis ipsilon has emerged as an excellent model for studying olfactory processing and its plasticity in the central nervous system. Odor-evoked responses of AL neurons and input-to-output transformations involved in pheromone processing are well characterized in this species. However, the intrinsic electrical properties responsible of the firing of AL neurons are poorly known. To this end, patch-clamp recordings in current- and voltage-clamp mode from neurons located in the two main clusters of cell bodies in the ALs were combined with intracellular staining on A. ipsilon males. Staining indicated that the lateral cluster (LC) is composed of 85% of local neurons (LNs) and 15% of projection neurons (PNs). The medial cluster (MC) contains only PNs. Action potentials were readily recorded from the soma in LNs and PNs located in the LC but not from PNs in the MC where recordings showed small or no action potentials. In the LC, the spontaneous activity of about 20% of the LNs presented irregular bursts while being more regular in PNs. We also identified a small population of LNs lacking voltage-gated Na(+) currents and generating spikelets. We focused on the firing properties of LNs since in about 60% of LNs, but not in PNs, action potentials were followed by depolarizing afterpotentials (DAPs). These DAPs could generate a second action potential, so that the activity was composed of action potential doublets. DAPs depended on voltage, Ca(2+)-channels and possibly on Ca(2+)-activated non-specific cationic channels. During steady state current injection, DAPs occurred after each action potential and did not require high-frequency firing. The amplitude of DAPs increased when the interspike interval was small, typically within bursts, likely arising from a Ca(2+) build up. DAPs were more often found in bursting than in non-bursting LNs but do not support bursting activity. DAPs and spike doublets also occurred during odor-evoked activity suggesting that they can mediate olfactory integration in the AL.</AbstractText><CopyrightInformation>Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lavialle-Defaix</LastName><ForeName>Céline</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>UMR 1392 Institute of Ecology and Environmental Sciences of Paris (iEES-Paris), INRA, Route de Saint-Cyr, F-78026 Versailles cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jacob</LastName><ForeName>Vincent</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>UMR 1392 Institute of Ecology and Environmental Sciences of Paris (iEES-Paris), INRA, Route de Saint-Cyr, F-78026 Versailles cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Monsempès</LastName><ForeName>Christelle</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>UMR 1392 Institute of Ecology and Environmental Sciences of Paris (iEES-Paris), INRA, Route de Saint-Cyr, F-78026 Versailles cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Anton</LastName><ForeName>Sylvia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Neuroéthologie-RCIM, INRA-Université d'Angers, UPRES EA 2647 USC INRA 1330, 42 rue Georges Morel, 49071 Beaucouzé, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rospars</LastName><ForeName>Jean-Pierre</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>UMR 1392 Institute of Ecology and Environmental Sciences of Paris (iEES-Paris), INRA, Route de Saint-Cyr, F-78026 Versailles cedex, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Martinez</LastName><ForeName>Dominique</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>UMR7503, Laboratoire Lorrain de Recherche en Informatique et ses Applications (LORIA), Centre National de la Recherche Scientifique (CNRS), Vandœuvre-lès-Nancy, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lucas</LastName><ForeName>Philippe</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>UMR 1392 Institute of Ecology and Environmental Sciences of Paris (iEES-Paris), INRA, Route de Saint-Cyr, F-78026 Versailles cedex, France. Electronic address: philippe.lucas@versailles.inra.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>06</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Ireland</Country><MedlineTA>Biosystems</MedlineTA><NlmUniqueID>0430773</NlmUniqueID><ISSNLinking>0303-2647</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antennal lobe</Keyword><Keyword MajorTopicYN="N">Depolarizing afterpotential</Keyword><Keyword MajorTopicYN="N">Firing</Keyword><Keyword MajorTopicYN="N">Local interneuron</Keyword><Keyword MajorTopicYN="N">Olfaction</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>3</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>6</Month><Day>4</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>6</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2015</Year><Month>6</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>7</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26126723</ArticleId><ArticleId IdType="pii">S0303-2647(15)00088-X</ArticleId><ArticleId IdType="doi">10.1016/j.biosystems.2015.06.005</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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