ChansonRolandV6, Main, Exploration, bibRecord, 001A84

Nonsense suppression in thymine-requiring strains of Escherichia coli is a consequence of altered folate metabolism

Identifieur interne : 001A84 ( Main/Exploration ); précédent : 001A83; suivant : 001A85

Nonsense suppression in thymine-requiring strains of Escherichia coli is a consequence of altered folate metabolism

Auteurs : Johnny Basso [Canada] ; Evangelos Tiganos [Canada] ; Muriel B. Herrington [Canada]

Source :

Molecular and General Genetics MGG [ 0026-8925 ] ; 1993.

RBID : ISTEX:E9D48F0160273203BC272D626692C0628BE254FE

English descriptors

Entity :
- org : Canlab Scientific Products, Concordia University, Fisher Scientific, Sigma Chemical Company.
- pers : A. Bognar, Alfred Noubani, Claire Cupples, E. Howell, Evangelos Tiganos, I. Suppression, Johnny Basso, M. Herrington, M. Singer, Pak, Thy GlyA, Zhong Qi.
- place : Canada, Quebec.
Teeft :
- American society, Aminopterin, Antifolate drug, Assay, Biosynthesis, Bognar, Burst size, Burst size assay, Casei, Cell extract, Cell growth, Coli, Dihydrofolate, Dihydrofolate reductase, Escherichia, Escherichia coli, Final concentration, Fola, Folate, Folate content, Folate level, Folate metabolism, Folc, Folc gene, Fpgs activity, Glya, Glycine, Herrington, Independent experiment, Minimal medium, Molecular biology, Mutant, Mutant phage, Mutation, Other folate, Phage, Plaque formation, Plasmid, Plasmid pac5, Plating, Plating efficiency, Relative burst size, Standard deviation, Sulfathiazole, Suppression, Suppressor, Synthase, Tail fiber protein, Tetrahydrofolate, Thya, Thymidine, Thymidylate, Thymidylate synthase, Total folate, Translational, Translational accuracy, Trimethoprim, Trna.

Abstract

Abstract: Thymine-requiring strains of Escherichia coli suppress nonsense and frameshift mutants of T4 phage. We proposed that these mutants make errors during translation because of an imbalance in folate metabolism. A thymine-requiring strain grown under suppressing conditions has elevated levels of reduced folates. We tested the effect of either mutational blocks or the inhibition of various steps in folate biosynthesis on suppression. Conditions which prevent the accumulation of 5-methyl tetrahydrofolate inhibit suppression, suggesting that elevated levels of this folate are required for suppression. Furthermore, conditions that result in an accumulation in dihydrofolate inhibit suppression.

Url:

https://api.istex.fr/ark:/67375/1BB-2FZ9QVJ9-K/fulltext.pdf

DOI: 10.1007/BF00279550

Affiliations:

Canada

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 002208
to stream Istex, to step Curation: 002052
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to stream Main, to step Curation: 001A84

Le document en format XML

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<term>I. Suppression</term>
<term>Johnny Basso</term>
<term>M. Herrington</term>
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<term>Thya</term>
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<term>Thymidylate</term>
<term>Thymidylate synthase</term>
<term>Total folate</term>
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<term>Translational accuracy</term>
<term>Trimethoprim</term>
<term>Trna</term>
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<front><div type="abstract" xml:lang="en">Abstract: Thymine-requiring strains of Escherichia coli suppress nonsense and frameshift mutants of T4 phage. We proposed that these mutants make errors during translation because of an imbalance in folate metabolism. A thymine-requiring strain grown under suppressing conditions has elevated levels of reduced folates. We tested the effect of either mutational blocks or the inhibition of various steps in folate biosynthesis on suppression. Conditions which prevent the accumulation of 5-methyl tetrahydrofolate inhibit suppression, suggesting that elevated levels of this folate are required for suppression. Furthermore, conditions that result in an accumulation in dihydrofolate inhibit suppression.</div>
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Nonsense suppression in thymine-requiring strains of Escherichia coli is a consequence of altered folate metabolism

Nonsense suppression in thymine-requiring strains of Escherichia coli is a consequence of altered folate metabolism

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri