Restoration of Progranulin Expression Rescues Cortical Neuron Generation in an Induced Pluripotent Stem Cell Model of Frontotemporal Dementia
Identifieur interne : 000261 ( Pmc/Corpus ); précédent : 000260; suivant : 000262Restoration of Progranulin Expression Rescues Cortical Neuron Generation in an Induced Pluripotent Stem Cell Model of Frontotemporal Dementia
Auteurs : Susanna Raitano ; Laura Ordovàs ; Louis De Muynck ; Wenting Guo ; Ira Espuny-Camacho ; Martine Geraerts ; Satish Khurana ; Kim Vanuytsel ; Balazs I. T Th ; Thomas Voets ; Rik Vandenberghe ; Toni Cathomen ; Ludo Van Den Bosch ; Pierre Vanderhaeghen ; Philip Van Damme ; Catherine M. VerfaillieSource :
- Stem Cell Reports [ 2213-6711 ] ; 2014.
Abstract
To understand how haploinsufficiency of progranulin (PGRN) causes frontotemporal dementia (FTD), we created induced pluripotent stem cells (iPSCs) from patients carrying the
Url:
DOI: 10.1016/j.stemcr.2014.12.001
PubMed: 25556567
PubMed Central: 4297877
Links to Exploration step
PMC:4297877Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Restoration of Progranulin Expression Rescues Cortical Neuron Generation in an Induced Pluripotent Stem Cell Model of Frontotemporal Dementia</title><author><name sortKey="Raitano, Susanna" sort="Raitano, Susanna" uniqKey="Raitano S" first="Susanna" last="Raitano">Susanna Raitano</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Ordovas, Laura" sort="Ordovas, Laura" uniqKey="Ordovas L" first="Laura" last="Ordovàs">Laura Ordovàs</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="De Muynck, Louis" sort="De Muynck, Louis" uniqKey="De Muynck L" first="Louis" last="De Muynck">Louis De Muynck</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Laboratory of Neurobiology, VIB Vesalius Research Center, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Guo, Wenting" sort="Guo, Wenting" uniqKey="Guo W" first="Wenting" last="Guo">Wenting Guo</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Laboratory of Neurobiology, VIB Vesalius Research Center, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Espuny Camacho, Ira" sort="Espuny Camacho, Ira" uniqKey="Espuny Camacho I" first="Ira" last="Espuny-Camacho">Ira Espuny-Camacho</name><affiliation><nlm:aff id="aff6">Institute for Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff7">Institute of Neuroscience (UNI), ULB, 1070 Brussels, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">VIB Center for the Biology of Disease, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Center of Human Genetics, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Geraerts, Martine" sort="Geraerts, Martine" uniqKey="Geraerts M" first="Martine" last="Geraerts">Martine Geraerts</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Khurana, Satish" sort="Khurana, Satish" uniqKey="Khurana S" first="Satish" last="Khurana">Satish Khurana</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Vanuytsel, Kim" sort="Vanuytsel, Kim" uniqKey="Vanuytsel K" first="Kim" last="Vanuytsel">Kim Vanuytsel</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="T Th, Balazs I" sort="T Th, Balazs I" uniqKey="T Th B" first="Balazs I." last="T Th">Balazs I. T Th</name><affiliation><nlm:aff id="aff10">Laboratory of Ion Channel Research, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Voets, Thomas" sort="Voets, Thomas" uniqKey="Voets T" first="Thomas" last="Voets">Thomas Voets</name><affiliation><nlm:aff id="aff10">Laboratory of Ion Channel Research, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Vandenberghe, Rik" sort="Vandenberghe, Rik" uniqKey="Vandenberghe R" first="Rik" last="Vandenberghe">Rik Vandenberghe</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff14">Department of Neurology, University Hospitals Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Cathomen, Toni" sort="Cathomen, Toni" uniqKey="Cathomen T" first="Toni" last="Cathomen">Toni Cathomen</name><affiliation><nlm:aff id="aff11">Institute for Cell and Gene Therapy, University Medical Center, 79108 Freiburg, Germany</nlm:aff></affiliation><affiliation><nlm:aff id="aff12">Center for Chronic Immunodeficiency, University Medical Center, 79108 Freiburg, Germany</nlm:aff></affiliation></author><author><name sortKey="Van Den Bosch, Ludo" sort="Van Den Bosch, Ludo" uniqKey="Van Den Bosch L" first="Ludo" last="Van Den Bosch">Ludo Van Den Bosch</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Laboratory of Neurobiology, VIB Vesalius Research Center, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Vanderhaeghen, Pierre" sort="Vanderhaeghen, Pierre" uniqKey="Vanderhaeghen P" first="Pierre" last="Vanderhaeghen">Pierre Vanderhaeghen</name><affiliation><nlm:aff id="aff6">Institute for Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff7">Institute of Neuroscience (UNI), ULB, 1070 Brussels, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">VIB Center for the Biology of Disease, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Center of Human Genetics, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff13">Walloon Excellence in Life Sciences and Biotechnology (WELBIO), 1070 Brussels, Belgium</nlm:aff></affiliation></author><author><name sortKey="Van Damme, Philip" sort="Van Damme, Philip" uniqKey="Van Damme P" first="Philip" last="Van Damme">Philip Van Damme</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Laboratory of Neurobiology, VIB Vesalius Research Center, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff14">Department of Neurology, University Hospitals Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Verfaillie, Catherine M" sort="Verfaillie, Catherine M" uniqKey="Verfaillie C" first="Catherine M." last="Verfaillie">Catherine M. Verfaillie</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25556567</idno><idno type="pmc">4297877</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4297877</idno><idno type="RBID">PMC:4297877</idno><idno type="doi">10.1016/j.stemcr.2014.12.001</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000261</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Restoration of Progranulin Expression Rescues Cortical Neuron Generation in an Induced Pluripotent Stem Cell Model of Frontotemporal Dementia</title><author><name sortKey="Raitano, Susanna" sort="Raitano, Susanna" uniqKey="Raitano S" first="Susanna" last="Raitano">Susanna Raitano</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Ordovas, Laura" sort="Ordovas, Laura" uniqKey="Ordovas L" first="Laura" last="Ordovàs">Laura Ordovàs</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="De Muynck, Louis" sort="De Muynck, Louis" uniqKey="De Muynck L" first="Louis" last="De Muynck">Louis De Muynck</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Laboratory of Neurobiology, VIB Vesalius Research Center, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Guo, Wenting" sort="Guo, Wenting" uniqKey="Guo W" first="Wenting" last="Guo">Wenting Guo</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Laboratory of Neurobiology, VIB Vesalius Research Center, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Espuny Camacho, Ira" sort="Espuny Camacho, Ira" uniqKey="Espuny Camacho I" first="Ira" last="Espuny-Camacho">Ira Espuny-Camacho</name><affiliation><nlm:aff id="aff6">Institute for Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff7">Institute of Neuroscience (UNI), ULB, 1070 Brussels, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">VIB Center for the Biology of Disease, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Center of Human Genetics, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Geraerts, Martine" sort="Geraerts, Martine" uniqKey="Geraerts M" first="Martine" last="Geraerts">Martine Geraerts</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Khurana, Satish" sort="Khurana, Satish" uniqKey="Khurana S" first="Satish" last="Khurana">Satish Khurana</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Vanuytsel, Kim" sort="Vanuytsel, Kim" uniqKey="Vanuytsel K" first="Kim" last="Vanuytsel">Kim Vanuytsel</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="T Th, Balazs I" sort="T Th, Balazs I" uniqKey="T Th B" first="Balazs I." last="T Th">Balazs I. T Th</name><affiliation><nlm:aff id="aff10">Laboratory of Ion Channel Research, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Voets, Thomas" sort="Voets, Thomas" uniqKey="Voets T" first="Thomas" last="Voets">Thomas Voets</name><affiliation><nlm:aff id="aff10">Laboratory of Ion Channel Research, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Vandenberghe, Rik" sort="Vandenberghe, Rik" uniqKey="Vandenberghe R" first="Rik" last="Vandenberghe">Rik Vandenberghe</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff14">Department of Neurology, University Hospitals Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Cathomen, Toni" sort="Cathomen, Toni" uniqKey="Cathomen T" first="Toni" last="Cathomen">Toni Cathomen</name><affiliation><nlm:aff id="aff11">Institute for Cell and Gene Therapy, University Medical Center, 79108 Freiburg, Germany</nlm:aff></affiliation><affiliation><nlm:aff id="aff12">Center for Chronic Immunodeficiency, University Medical Center, 79108 Freiburg, Germany</nlm:aff></affiliation></author><author><name sortKey="Van Den Bosch, Ludo" sort="Van Den Bosch, Ludo" uniqKey="Van Den Bosch L" first="Ludo" last="Van Den Bosch">Ludo Van Den Bosch</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Laboratory of Neurobiology, VIB Vesalius Research Center, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Vanderhaeghen, Pierre" sort="Vanderhaeghen, Pierre" uniqKey="Vanderhaeghen P" first="Pierre" last="Vanderhaeghen">Pierre Vanderhaeghen</name><affiliation><nlm:aff id="aff6">Institute for Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff7">Institute of Neuroscience (UNI), ULB, 1070 Brussels, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">VIB Center for the Biology of Disease, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff9">Center of Human Genetics, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff13">Walloon Excellence in Life Sciences and Biotechnology (WELBIO), 1070 Brussels, Belgium</nlm:aff></affiliation></author><author><name sortKey="Van Damme, Philip" sort="Van Damme, Philip" uniqKey="Van Damme P" first="Philip" last="Van Damme">Philip Van Damme</name><affiliation><nlm:aff id="aff3">Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Laboratory of Neurobiology, VIB Vesalius Research Center, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff14">Department of Neurology, University Hospitals Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author><author><name sortKey="Verfaillie, Catherine M" sort="Verfaillie, Catherine M" uniqKey="Verfaillie C" first="Catherine M." last="Verfaillie">Catherine M. Verfaillie</name><affiliation><nlm:aff id="aff1">Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</nlm:aff></affiliation></author></analytic><series><title level="j">Stem Cell Reports</title><idno type="eISSN">2213-6711</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p>To understand how haploinsufficiency of progranulin (PGRN) causes frontotemporal dementia (FTD), we created induced pluripotent stem cells (iPSCs) from patients carrying the <italic>GRN</italic><sup>IVS1+5G > C</sup> mutation (FTD-iPSCs). FTD-iPSCs were fated to cortical neurons, the cells most affected in FTD. Although generation of neuroprogenitors was unaffected, their further differentiation into CTIP2-, FOXP2-, or TBR1-TUJ1 double-positive cortical neurons, but not motorneurons, was significantly decreased in FTD-neural progeny. Zinc finger nuclease-mediated introduction of <italic>GRN</italic> cDNA into the <italic>AAVS1</italic> locus corrected defects in cortical neurogenesis, demonstrating that PGRN haploinsufficiency causes inefficient cortical neuron generation. RNA sequencing analysis confirmed reversal of the altered gene expression profile following genetic correction. We identified the Wnt signaling pathway as one of the top defective pathways in FTD-iPSC-derived neurons, which was reversed following genetic correction. Differentiation of FTD-iPSCs in the presence of a WNT inhibitor mitigated defective corticogenesis. Therefore, we demonstrate that PGRN haploinsufficiency hampers corticogenesis in vitro.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Almeida, S" uniqKey="Almeida S">S. Almeida</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z. Zhang</name></author><author><name sortKey="Coppola, G" uniqKey="Coppola G">G. Coppola</name></author><author><name sortKey="Mao, W" uniqKey="Mao W">W. Mao</name></author><author><name sortKey="Futai, K" uniqKey="Futai K">K. Futai</name></author><author><name sortKey="Karydas, A" uniqKey="Karydas A">A. Karydas</name></author><author><name sortKey="Geschwind, M D" uniqKey="Geschwind M">M.D. Geschwind</name></author><author><name sortKey="Tartaglia, M C" uniqKey="Tartaglia M">M.C. Tartaglia</name></author><author><name sortKey="Gao, F" uniqKey="Gao F">F. Gao</name></author><author><name sortKey="Gianni, D" uniqKey="Gianni D">D. Gianni</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baker, M" uniqKey="Baker M">M. Baker</name></author><author><name sortKey="Mackenzie, I R" uniqKey="Mackenzie I">I.R. Mackenzie</name></author><author><name sortKey="Pickering Brown, S M" uniqKey="Pickering Brown S">S.M. Pickering-Brown</name></author><author><name sortKey="Gass, J" uniqKey="Gass J">J. Gass</name></author><author><name sortKey="Rademakers, R" uniqKey="Rademakers R">R. Rademakers</name></author><author><name sortKey="Lindholm, C" uniqKey="Lindholm C">C. Lindholm</name></author><author><name sortKey="Snowden, J" uniqKey="Snowden J">J. Snowden</name></author><author><name sortKey="Adamson, J" uniqKey="Adamson J">J. Adamson</name></author><author><name sortKey="Sadovnick, A D" uniqKey="Sadovnick A">A.D. Sadovnick</name></author><author><name sortKey="Rollinson, S" uniqKey="Rollinson S">S. Rollinson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cruts, M" uniqKey="Cruts M">M. Cruts</name></author><author><name sortKey="Gijselinck, I" uniqKey="Gijselinck I">I. Gijselinck</name></author><author><name sortKey="Van Der Zee, J" uniqKey="Van Der Zee J">J. van der Zee</name></author><author><name sortKey="Engelborghs, S" uniqKey="Engelborghs S">S. Engelborghs</name></author><author><name sortKey="Wils, H" uniqKey="Wils H">H. Wils</name></author><author><name sortKey="Pirici, D" uniqKey="Pirici D">D. Pirici</name></author><author><name sortKey="Rademakers, R" uniqKey="Rademakers R">R. Rademakers</name></author><author><name sortKey="Vandenberghe, R" uniqKey="Vandenberghe R">R. Vandenberghe</name></author><author><name sortKey="Dermaut, B" uniqKey="Dermaut B">B. Dermaut</name></author><author><name sortKey="Martin, J J" uniqKey="Martin J">J.J. Martin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Muynck, L" uniqKey="De Muynck L">L. De Muynck</name></author><author><name sortKey="Herdewyn, S" uniqKey="Herdewyn S">S. Herdewyn</name></author><author><name sortKey="Beel, S" uniqKey="Beel S">S. Beel</name></author><author><name sortKey="Scheveneels, W" uniqKey="Scheveneels W">W. Scheveneels</name></author><author><name sortKey="Van Den Bosch, L" uniqKey="Van Den Bosch L">L. Van Den Bosch</name></author><author><name sortKey="Robberecht, W" uniqKey="Robberecht W">W. Robberecht</name></author><author><name sortKey="Van Damme, P" uniqKey="Van Damme P">P. Van Damme</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dejesus Hernandez, M" uniqKey="Dejesus Hernandez M">M. DeJesus-Hernandez</name></author><author><name sortKey="Mackenzie, I R" uniqKey="Mackenzie I">I.R. Mackenzie</name></author><author><name sortKey="Boeve, B F" uniqKey="Boeve B">B.F. Boeve</name></author><author><name sortKey="Boxer, A L" uniqKey="Boxer A">A.L. Boxer</name></author><author><name sortKey="Baker, M" uniqKey="Baker M">M. Baker</name></author><author><name sortKey="Rutherford, N J" uniqKey="Rutherford N">N.J. Rutherford</name></author><author><name sortKey="Nicholson, A M" uniqKey="Nicholson A">A.M. Nicholson</name></author><author><name sortKey="Finch, N A" uniqKey="Finch N">N.A. Finch</name></author><author><name sortKey="Flynn, H" uniqKey="Flynn H">H. Flynn</name></author><author><name sortKey="Adamson, J" uniqKey="Adamson J">J. Adamson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Espuny Camacho, I" uniqKey="Espuny Camacho I">I. Espuny-Camacho</name></author><author><name sortKey="Michelsen, K A" uniqKey="Michelsen K">K.A. Michelsen</name></author><author><name sortKey="Gall, D" uniqKey="Gall D">D. Gall</name></author><author><name sortKey="Linaro, D" uniqKey="Linaro D">D. Linaro</name></author><author><name sortKey="Hasche, A" uniqKey="Hasche A">A. Hasche</name></author><author><name sortKey="Bonnefont, J" uniqKey="Bonnefont J">J. Bonnefont</name></author><author><name sortKey="Bali, C" uniqKey="Bali C">C. Bali</name></author><author><name sortKey="Orduz, D" uniqKey="Orduz D">D. Orduz</name></author><author><name sortKey="Bilheu, A" uniqKey="Bilheu A">A. Bilheu</name></author><author><name sortKey="Herpoel, A" uniqKey="Herpoel A">A. Herpoel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gao, X" uniqKey="Gao X">X. Gao</name></author><author><name sortKey="Joselin, A P" uniqKey="Joselin A">A.P. Joselin</name></author><author><name sortKey="Wang, L" uniqKey="Wang L">L. Wang</name></author><author><name sortKey="Kar, A" uniqKey="Kar A">A. Kar</name></author><author><name sortKey="Ray, P" uniqKey="Ray P">P. Ray</name></author><author><name sortKey="Bateman, A" uniqKey="Bateman A">A. Bateman</name></author><author><name sortKey="Goate, A M" uniqKey="Goate A">A.M. Goate</name></author><author><name sortKey="Wu, J Y" uniqKey="Wu J">J.Y. Wu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gass, J" uniqKey="Gass J">J. Gass</name></author><author><name sortKey="Lee, W C" uniqKey="Lee W">W.C. Lee</name></author><author><name sortKey="Cook, C" uniqKey="Cook C">C. Cook</name></author><author><name sortKey="Finch, N" uniqKey="Finch N">N. Finch</name></author><author><name sortKey="Stetler, C" uniqKey="Stetler C">C. Stetler</name></author><author><name sortKey="Jansen West, K" uniqKey="Jansen West K">K. Jansen-West</name></author><author><name sortKey="Lewis, J" uniqKey="Lewis J">J. Lewis</name></author><author><name sortKey="Link, C D" uniqKey="Link C">C.D. Link</name></author><author><name sortKey="Rademakers, R" uniqKey="Rademakers R">R. Rademakers</name></author><author><name sortKey="Nykj R, A" uniqKey="Nykj R A">A. Nykjær</name></author><author><name sortKey="Petrucelli, L" uniqKey="Petrucelli L">L. Petrucelli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goldman, J S" uniqKey="Goldman J">J.S. Goldman</name></author><author><name sortKey="Farmer, J M" uniqKey="Farmer J">J.M. Farmer</name></author><author><name sortKey="Wood, E M" uniqKey="Wood E">E.M. Wood</name></author><author><name sortKey="Johnson, J K" uniqKey="Johnson J">J.K. Johnson</name></author><author><name sortKey="Boxer, A" uniqKey="Boxer A">A. Boxer</name></author><author><name sortKey="Neuhaus, J" uniqKey="Neuhaus J">J. Neuhaus</name></author><author><name sortKey="Lomen Hoerth, C" uniqKey="Lomen Hoerth C">C. Lomen-Hoerth</name></author><author><name sortKey="Wilhelmsen, K C" uniqKey="Wilhelmsen K">K.C. Wilhelmsen</name></author><author><name sortKey="Lee, V M" uniqKey="Lee V">V.M. Lee</name></author><author><name sortKey="Grossman, M" uniqKey="Grossman M">M. Grossman</name></author><author><name sortKey="Miller, B L" uniqKey="Miller B">B.L. Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, B Y" uniqKey="Hu B">B.Y. Hu</name></author><author><name sortKey="Zhang, S C" uniqKey="Zhang S">S.C. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hutton, M" uniqKey="Hutton M">M. Hutton</name></author><author><name sortKey="Lendon, C L" uniqKey="Lendon C">C.L. Lendon</name></author><author><name sortKey="Rizzu, P" uniqKey="Rizzu P">P. Rizzu</name></author><author><name sortKey="Baker, M" uniqKey="Baker M">M. Baker</name></author><author><name sortKey="Froelich, S" uniqKey="Froelich S">S. Froelich</name></author><author><name sortKey="Houlden, H" uniqKey="Houlden H">H. Houlden</name></author><author><name sortKey="Pickering Brown, S" uniqKey="Pickering Brown S">S. Pickering-Brown</name></author><author><name sortKey="Chakraverty, S" uniqKey="Chakraverty S">S. Chakraverty</name></author><author><name sortKey="Isaacs, A" uniqKey="Isaacs A">A. Isaacs</name></author><author><name sortKey="Grover, A" uniqKey="Grover A">A. Grover</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kayasuga, Y" uniqKey="Kayasuga Y">Y. Kayasuga</name></author><author><name sortKey="Chiba, S" uniqKey="Chiba S">S. Chiba</name></author><author><name sortKey="Suzuki, M" uniqKey="Suzuki M">M. Suzuki</name></author><author><name sortKey="Kikusui, T" uniqKey="Kikusui T">T. Kikusui</name></author><author><name sortKey="Matsuwaki, T" uniqKey="Matsuwaki T">T. Matsuwaki</name></author><author><name sortKey="Yamanouchi, K" uniqKey="Yamanouchi K">K. Yamanouchi</name></author><author><name sortKey="Kotaki, H" uniqKey="Kotaki H">H. Kotaki</name></author><author><name sortKey="Horai, R" uniqKey="Horai R">R. Horai</name></author><author><name sortKey="Iwakura, Y" uniqKey="Iwakura Y">Y. Iwakura</name></author><author><name sortKey="Nishihara, M" uniqKey="Nishihara M">M. Nishihara</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, C" uniqKey="Kim C">C. Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Korade, Z" uniqKey="Korade Z">Z. Korade</name></author><author><name sortKey="Mirnics, K" uniqKey="Mirnics K">K. Mirnics</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, H L" uniqKey="Li H">H.L. Li</name></author><author><name sortKey="Wang, H H" uniqKey="Wang H">H.H. Wang</name></author><author><name sortKey="Liu, S J" uniqKey="Liu S">S.J. Liu</name></author><author><name sortKey="Deng, Y Q" uniqKey="Deng Y">Y.Q. Deng</name></author><author><name sortKey="Zhang, Y J" uniqKey="Zhang Y">Y.J. Zhang</name></author><author><name sortKey="Tian, Q" uniqKey="Tian Q">Q. Tian</name></author><author><name sortKey="Wang, X C" uniqKey="Wang X">X.C. Wang</name></author><author><name sortKey="Chen, X Q" uniqKey="Chen X">X.Q. Chen</name></author><author><name sortKey="Yang, Y" uniqKey="Yang Y">Y. Yang</name></author><author><name sortKey="Zhang, J Y" uniqKey="Zhang J">J.Y. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ming, G L" uniqKey="Ming G">G.L. Ming</name></author><author><name sortKey="Song, H" uniqKey="Song H">H. Song</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Miranda, C J" uniqKey="Miranda C">C.J. Miranda</name></author><author><name sortKey="Braun, L" uniqKey="Braun L">L. Braun</name></author><author><name sortKey="Jiang, Y" uniqKey="Jiang Y">Y. Jiang</name></author><author><name sortKey="Hester, M E" uniqKey="Hester M">M.E. Hester</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L. Zhang</name></author><author><name sortKey="Riolo, M" uniqKey="Riolo M">M. Riolo</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author><author><name sortKey="Rao, M" uniqKey="Rao M">M. Rao</name></author><author><name sortKey="Altura, R A" uniqKey="Altura R">R.A. Altura</name></author><author><name sortKey="Kaspar, B K" uniqKey="Kaspar B">B.K. Kaspar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Neary, D" uniqKey="Neary D">D. Neary</name></author><author><name sortKey="Snowden, J" uniqKey="Snowden J">J. Snowden</name></author><author><name sortKey="Mann, D" uniqKey="Mann D">D. Mann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ong, C H" uniqKey="Ong C">C.H. Ong</name></author><author><name sortKey="He, Z" uniqKey="He Z">Z. He</name></author><author><name sortKey="Kriazhev, L" uniqKey="Kriazhev L">L. Kriazhev</name></author><author><name sortKey="Shan, X" uniqKey="Shan X">X. Shan</name></author><author><name sortKey="Palfree, R G" uniqKey="Palfree R">R.G. Palfree</name></author><author><name sortKey="Bateman, A" uniqKey="Bateman A">A. Bateman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qiang, L" uniqKey="Qiang L">L. Qiang</name></author><author><name sortKey="Fujita, R" uniqKey="Fujita R">R. Fujita</name></author><author><name sortKey="Abeliovich, A" uniqKey="Abeliovich A">A. Abeliovich</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Renton, A E" uniqKey="Renton A">A.E. Renton</name></author><author><name sortKey="Majounie, E" uniqKey="Majounie E">E. Majounie</name></author><author><name sortKey="Waite, A" uniqKey="Waite A">A. Waite</name></author><author><name sortKey="Sim N Sanchez, J" uniqKey="Sim N Sanchez J">J. Simón-Sánchez</name></author><author><name sortKey="Rollinson, S" uniqKey="Rollinson S">S. Rollinson</name></author><author><name sortKey="Gibbs, J R" uniqKey="Gibbs J">J.R. Gibbs</name></author><author><name sortKey="Schymick, J C" uniqKey="Schymick J">J.C. Schymick</name></author><author><name sortKey="Laaksovirta, H" uniqKey="Laaksovirta H">H. Laaksovirta</name></author><author><name sortKey="Van Swieten, J C" uniqKey="Van Swieten J">J.C. van Swieten</name></author><author><name sortKey="Myllykangas, L" uniqKey="Myllykangas L">L. Myllykangas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rosen, E Y" uniqKey="Rosen E">E.Y. Rosen</name></author><author><name sortKey="Wexler, E M" uniqKey="Wexler E">E.M. Wexler</name></author><author><name sortKey="Versano, R" uniqKey="Versano R">R. Versano</name></author><author><name sortKey="Coppola, G" uniqKey="Coppola G">G. Coppola</name></author><author><name sortKey="Gao, F" uniqKey="Gao F">F. Gao</name></author><author><name sortKey="Winden, K D" uniqKey="Winden K">K.D. Winden</name></author><author><name sortKey="Oldham, M C" uniqKey="Oldham M">M.C. Oldham</name></author><author><name sortKey="Martens, L H" uniqKey="Martens L">L.H. Martens</name></author><author><name sortKey="Zhou, P" uniqKey="Zhou P">P. Zhou</name></author><author><name sortKey="Farese, R V" uniqKey="Farese R">R.V. Farese</name></author><author><name sortKey="Geschwind, D H" uniqKey="Geschwind D">D.H. Geschwind</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takahashi, K" uniqKey="Takahashi K">K. Takahashi</name></author><author><name sortKey="Okita, K" uniqKey="Okita K">K. Okita</name></author><author><name sortKey="Nakagawa, M" uniqKey="Nakagawa M">M. Nakagawa</name></author><author><name sortKey="Yamanaka, S" uniqKey="Yamanaka S">S. Yamanaka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takahashi, K" uniqKey="Takahashi K">K. Takahashi</name></author><author><name sortKey="Tanabe, K" uniqKey="Tanabe K">K. Tanabe</name></author><author><name sortKey="Ohnuki, M" uniqKey="Ohnuki M">M. Ohnuki</name></author><author><name sortKey="Narita, M" uniqKey="Narita M">M. Narita</name></author><author><name sortKey="Ichisaka, T" uniqKey="Ichisaka T">T. Ichisaka</name></author><author><name sortKey="Tomoda, K" uniqKey="Tomoda K">K. Tomoda</name></author><author><name sortKey="Yamanaka, S" uniqKey="Yamanaka S">S. Yamanaka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Damme, P" uniqKey="Van Damme P">P. Van Damme</name></author><author><name sortKey="Van Hoecke, A" uniqKey="Van Hoecke A">A. Van Hoecke</name></author><author><name sortKey="Lambrechts, D" uniqKey="Lambrechts D">D. Lambrechts</name></author><author><name sortKey="Vanacker, P" uniqKey="Vanacker P">P. Vanacker</name></author><author><name sortKey="Bogaert, E" uniqKey="Bogaert E">E. Bogaert</name></author><author><name sortKey="Van Swieten, J" uniqKey="Van Swieten J">J. van Swieten</name></author><author><name sortKey="Carmeliet, P" uniqKey="Carmeliet P">P. Carmeliet</name></author><author><name sortKey="Van Den Bosch, L" uniqKey="Van Den Bosch L">L. Van Den Bosch</name></author><author><name sortKey="Robberecht, W" uniqKey="Robberecht W">W. Robberecht</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Swieten, J C" uniqKey="Van Swieten J">J.C. van Swieten</name></author><author><name sortKey="Heutink, P" uniqKey="Heutink P">P. Heutink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wexler, E M" uniqKey="Wexler E">E.M. Wexler</name></author><author><name sortKey="Paucer, A" uniqKey="Paucer A">A. Paucer</name></author><author><name sortKey="Kornblum, H I" uniqKey="Kornblum H">H.I. Kornblum</name></author><author><name sortKey="Palmer, T D" uniqKey="Palmer T">T.D. Palmer</name></author><author><name sortKey="Geschwind, D H" uniqKey="Geschwind D">D.H. Geschwind</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="brief-report"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Stem Cell Reports</journal-id><journal-id journal-id-type="iso-abbrev">Stem Cell Reports</journal-id><journal-title-group><journal-title>Stem Cell Reports</journal-title></journal-title-group><issn pub-type="epub">2213-6711</issn><publisher><publisher-name>Elsevier</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25556567</article-id><article-id pub-id-type="pmc">4297877</article-id><article-id pub-id-type="publisher-id">S2213-6711(14)00358-0</article-id><article-id pub-id-type="doi">10.1016/j.stemcr.2014.12.001</article-id><article-categories><subj-group subj-group-type="heading"><subject>Report</subject></subj-group></article-categories><title-group><article-title>Restoration of Progranulin Expression Rescues Cortical Neuron Generation in an Induced Pluripotent Stem Cell Model of Frontotemporal Dementia</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Raitano</surname><given-names>Susanna</given-names></name><xref rid="aff1" ref-type="aff">1</xref><xref rid="aff2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Ordovàs</surname><given-names>Laura</given-names></name><xref rid="aff1" ref-type="aff">1</xref><xref rid="aff2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>De Muynck</surname><given-names>Louis</given-names></name><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref><xref rid="aff5" ref-type="aff">5</xref></contrib><contrib contrib-type="author"><name><surname>Guo</surname><given-names>Wenting</given-names></name><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref><xref rid="aff5" ref-type="aff">5</xref></contrib><contrib contrib-type="author"><name><surname>Espuny-Camacho</surname><given-names>Ira</given-names></name><xref rid="aff6" ref-type="aff">6</xref><xref rid="aff7" ref-type="aff">7</xref><xref rid="aff8" ref-type="aff">8</xref><xref rid="aff9" ref-type="aff">9</xref></contrib><contrib contrib-type="author"><name><surname>Geraerts</surname><given-names>Martine</given-names></name><xref rid="aff1" ref-type="aff">1</xref><xref rid="aff2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Khurana</surname><given-names>Satish</given-names></name><xref rid="aff1" ref-type="aff">1</xref><xref rid="aff2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Vanuytsel</surname><given-names>Kim</given-names></name><xref rid="aff1" ref-type="aff">1</xref><xref rid="aff2" ref-type="aff">2</xref></contrib><contrib contrib-type="author"><name><surname>Tóth</surname><given-names>Balazs I.</given-names></name><xref rid="aff10" ref-type="aff">10</xref></contrib><contrib contrib-type="author"><name><surname>Voets</surname><given-names>Thomas</given-names></name><xref rid="aff10" ref-type="aff">10</xref></contrib><contrib contrib-type="author"><name><surname>Vandenberghe</surname><given-names>Rik</given-names></name><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref><xref rid="aff14" ref-type="aff">14</xref></contrib><contrib contrib-type="author"><name><surname>Cathomen</surname><given-names>Toni</given-names></name><xref rid="aff11" ref-type="aff">11</xref><xref rid="aff12" ref-type="aff">12</xref></contrib><contrib contrib-type="author"><name><surname>Van Den Bosch</surname><given-names>Ludo</given-names></name><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref><xref rid="aff5" ref-type="aff">5</xref></contrib><contrib contrib-type="author"><name><surname>Vanderhaeghen</surname><given-names>Pierre</given-names></name><xref rid="aff6" ref-type="aff">6</xref><xref rid="aff7" ref-type="aff">7</xref><xref rid="aff8" ref-type="aff">8</xref><xref rid="aff9" ref-type="aff">9</xref><xref rid="aff13" ref-type="aff">13</xref></contrib><contrib contrib-type="author"><name><surname>Van Damme</surname><given-names>Philip</given-names></name><xref rid="aff3" ref-type="aff">3</xref><xref rid="aff4" ref-type="aff">4</xref><xref rid="aff5" ref-type="aff">5</xref><xref rid="aff14" ref-type="aff">14</xref><xref rid="fn1" ref-type="fn">15</xref></contrib><contrib contrib-type="author"><name><surname>Verfaillie</surname><given-names>Catherine M.</given-names></name><email>catherine.verfaillie@med.kuleuven.be</email><xref rid="aff1" ref-type="aff">1</xref><xref rid="aff2" ref-type="aff">2</xref><xref rid="fn1" ref-type="fn">15</xref><xref rid="cor1" ref-type="corresp">∗</xref></contrib></contrib-group><aff id="aff1"><label>1</label>Stem Cell Institute, KU Leuven, 3000 Leuven, Belgium</aff><aff id="aff2"><label>2</label>Stem Cell Biology and Embryology Unit, Department of Development and Regeneration, KU Leuven, 3000 Leuven, Belgium</aff><aff id="aff3"><label>3</label>Leuven Research Institute for Neuroscience and Disease (LIND), KU Leuven, 3000 Leuven, Belgium</aff><aff id="aff4"><label>4</label>Research Group Experimental Neurology, Department of Neurosciences, KU Leuven, 3000 Leuven, Belgium</aff><aff id="aff5"><label>5</label>Laboratory of Neurobiology, VIB Vesalius Research Center, 3000 Leuven, Belgium</aff><aff id="aff6"><label>6</label>Institute for Interdisciplinary Research (IRIBHM), Université Libre de Bruxelles (ULB), 1070 Brussels, Belgium</aff><aff id="aff7"><label>7</label>Institute of Neuroscience (UNI), ULB, 1070 Brussels, Belgium</aff><aff id="aff8"><label>8</label>VIB Center for the Biology of Disease, 3000 Leuven, Belgium</aff><aff id="aff9"><label>9</label>Center of Human Genetics, KU Leuven, 3000 Leuven, Belgium</aff><aff id="aff10"><label>10</label>Laboratory of Ion Channel Research, KU Leuven, 3000 Leuven, Belgium</aff><aff id="aff11"><label>11</label>Institute for Cell and Gene Therapy, University Medical Center, 79108 Freiburg, Germany</aff><aff id="aff12"><label>12</label>Center for Chronic Immunodeficiency, University Medical Center, 79108 Freiburg, Germany</aff><aff id="aff13"><label>13</label>Walloon Excellence in Life Sciences and Biotechnology (WELBIO), 1070 Brussels, Belgium</aff><aff id="aff14"><label>14</label>Department of Neurology, University Hospitals Leuven, 3000 Leuven, Belgium</aff><author-notes><corresp id="cor1"><label>∗</label>Corresponding author <email>catherine.verfaillie@med.kuleuven.be</email></corresp><fn id="fn1"><label>15</label><p>Co-senior author</p></fn></author-notes><pub-date pub-type="pmc-release"><day>31</day><month>12</month><year>2014</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="epub"><day>31</day><month>12</month><year>2014</year></pub-date><pub-date pub-type="collection"><day>13</day><month>1</month><year>2015</year></pub-date><volume>4</volume><issue>1</issue><fpage>16</fpage><lpage>24</lpage><history><date date-type="received"><day>4</day><month>7</month><year>2014</year></date><date date-type="rev-recd"><day>28</day><month>11</month><year>2014</year></date><date date-type="accepted"><day>1</day><month>12</month><year>2014</year></date></history><permissions><copyright-statement>© 2015 The Authors</copyright-statement><copyright-year>2015</copyright-year><license license-type="CC BY-NC-ND" xlink:href="http://creativecommons.org/licenses/by-nc-nd/3.0/"><license-p>This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).</license-p></license></permissions><abstract><title>Summary</title><p>To understand how haploinsufficiency of progranulin (PGRN) causes frontotemporal dementia (FTD), we created induced pluripotent stem cells (iPSCs) from patients carrying the <italic>GRN</italic><sup>IVS1+5G > C</sup> mutation (FTD-iPSCs). FTD-iPSCs were fated to cortical neurons, the cells most affected in FTD. Although generation of neuroprogenitors was unaffected, their further differentiation into CTIP2-, FOXP2-, or TBR1-TUJ1 double-positive cortical neurons, but not motorneurons, was significantly decreased in FTD-neural progeny. Zinc finger nuclease-mediated introduction of <italic>GRN</italic> cDNA into the <italic>AAVS1</italic> locus corrected defects in cortical neurogenesis, demonstrating that PGRN haploinsufficiency causes inefficient cortical neuron generation. RNA sequencing analysis confirmed reversal of the altered gene expression profile following genetic correction. We identified the Wnt signaling pathway as one of the top defective pathways in FTD-iPSC-derived neurons, which was reversed following genetic correction. Differentiation of FTD-iPSCs in the presence of a WNT inhibitor mitigated defective corticogenesis. Therefore, we demonstrate that PGRN haploinsufficiency hampers corticogenesis in vitro.</p></abstract><abstract abstract-type="graphical"><title>Graphical Abstract</title><fig id="undfig1" position="anchor"><graphic xlink:href="fx1"></graphic></fig></abstract><abstract abstract-type="author-highlights"><title>Highlights</title><p><list list-type="simple"><list-item id="u0010"><label>•</label><p>In vitro generation of cortical neurons from PGRN deficient FTD-iPSC is inefficient</p></list-item><list-item id="u0015"><label>•</label><p>Incorporation of <italic>GRN</italic> cDNA via ZFN technology rescues cortical neurons generation</p></list-item><list-item id="u0020"><label>•</label><p>WNT signaling may also play a major role in the defects observed</p></list-item></list></p></abstract><abstract abstract-type="teaser"><p>Verfaillie and colleagues describe the inefficient cortical neuron, but not motorneuron, generation, from FTD-patient-derived iPSCs carrying a mutation in the <italic>GRN</italic> gene. They show restoration of the defective phenotype following introduction of the <italic>GRN</italic> cDNA in FTD-iPSC using zinc finger nucleases and by inhibiting the WNT pathway.</p></abstract></article-meta></front><body><sec sec-type="intro" id="sec1"><title>Introduction</title><p>Frontotemporal dementia (FTD) accounts for ∼50% of dementia cases before the age of 60. Up to 40% of FTD patients have a familial history (<xref rid="bib9 bib26" ref-type="bibr">Goldman et al., 2005; van Swieten and Heutink, 2008</xref>) due to mutations in the microtubule-associated protein tau gene (<italic>MAPT</italic>), progranulin gene (<italic>GRN</italic>), or <italic>C9orf72</italic> gene (<xref rid="bib2 bib3 bib5 bib11 bib21" ref-type="bibr">Baker et al., 2006; Cruts et al., 2006; DeJesus-Hernandez et al., 2011; Hutton et al., 1998; Renton et al., 2011</xref>). The majority of FTD-causing mutations in <italic>GRN</italic> are predicted to result in functional null alleles, causing haploinsufficiency. Progranulin (PGRN) has neurotrophic function in vitro and in vivo. Although PGRN<sup>−/−</sup> mice are viable, they do not recapitulate all the features of FTD (<xref rid="bib12" ref-type="bibr">Kayasuga et al., 2007</xref>).</p><p>Human somatic cell reprogramming to a pluripotent state (induced pluripotent stem cells; iPSCs)(<xref rid="bib23" ref-type="bibr">Takahashi et al., 2007a</xref>) can create human disease models in vitro using patient-derived iPSCs (<xref rid="bib13" ref-type="bibr">Kim, 2014</xref>), including neurodegenerative diseases (<xref rid="bib20" ref-type="bibr">Qiang et al., 2013</xref>) and, specifically, FTD (<xref rid="bib1" ref-type="bibr">Almeida et al., 2012</xref>). Unlike in the published FTD-iPSC model that differentiated iPSCs to a mixture of neuronal cells, we evaluated cortical neuron development from FTD-patient-derived iPSCs, as FTD is characterized by selective neurodegeneration of the frontal and/or temporal cortex (<xref rid="bib18" ref-type="bibr">Neary et al., 2005</xref>). We demonstrate that FTD-iPSCs carrying a <italic>GRN</italic><sup>IVS1+5G > C</sup> mutation differ in their ability to generate cortical neurons from control lines (iPSCs and human embryonic stem cells; hESCs) and that genetic correction restores this differentiation defect.</p></sec><sec sec-type="results" id="sec2"><title>Results</title><sec id="sec2.1"><title>FTD-iPSCs Differentiation into Neuroprogenitors Is Normal</title><p>To study the effect of PGRN haploinsufficiency in human neurogenesis, iPSC lines were generated from three different patients carrying the <italic>GRN</italic><sup>IVS1+5G > C</sup> mutation (<xref rid="app2" ref-type="sec">Figure S1</xref>A available online) as previously described (<xref rid="bib24" ref-type="bibr">Takahashi et al., 2007b</xref>). The human embryonic stem cell line, H9 (H9-ESC), and iPSCs from normal donor fibroblasts (CTRL-iPSCs) were used as control lines (<xref rid="app2" ref-type="sec">Figures S1</xref>B–S1E). Transcript and protein levels of PGRN in FTD-iPSC lines were reduced, approximately 30% and 50%, respectively, compared to H9-hESCs and CTRL-iPSCs (<xref rid="app2" ref-type="sec">Figures S1</xref>F and S1G). We next induced cortical differentiation (<xref rid="bib6" ref-type="bibr">Espuny-Camacho et al., 2013</xref>), which induced an increase in transcripts for the neuroprogenitor genes <italic>SOX1</italic>, <italic>PAX6</italic>, and <italic>FABP7</italic> (<italic>BLBP</italic>), with concomitant decrease in <italic>OCT4</italic> expression in day (d)24 FTD-iPSCs as well as CTRL-iPSC and H9-hESC progeny (<xref rid="app2" ref-type="sec">Figure S2</xref>A). Immunostaining confirmed that d24 neuroprogenitors did not express OCT4. Nearly 100% of the progeny of all lines were positive for the neuroectoderm-specific NESTIN marker, with a PAX6-positive dorsal fate, and stained positive for BLBP and OTX1-2 (<xref rid="app2" ref-type="sec">Figure S2</xref>B). Thus, neuroprogenitor formation from FTD-iPSCs appeared normal.</p></sec><sec id="sec2.2"><title>Inefficient Cortical Neuron Formation from FTD-iPSCs</title><p>We next allowed the neuroprogenitors to mature into cortical neurons. <italic>GRN</italic> mRNA levels in FTD cells during differentiation were approximately 50% compared to control lines (<xref rid="fig1" ref-type="fig">Figure 1</xref>A). D40 progeny from CTRL- and FTD-iPSCs contained functional neurons based on whole-cell current-clamp analysis. FTD-iPSC neurons consistently fired action potentials in response to depolarizing current injections, similar to neurons from control cell lines (<xref rid="app2" ref-type="sec">Figures S2</xref>CI–S2CII). Whole-cell voltage-clamp recordings revealed time- and voltage-dependent currents during depolarizing voltage steps, consistent with functional voltage-gated Na<sup>+</sup> and K<sup>+</sup> channels (<xref rid="app2" ref-type="sec">Figure S2</xref>CIII). The cortical neurotransmitter GABA induced transmembrane currents in FTD-iPSC-derived neurons, exhibiting the typical features of ionotropic GABA<sub>A</sub> receptors (<xref rid="app2" ref-type="sec">Figure S2</xref>CIV). We also observed spontaneous action potential firing in FTD-iPSC neurons (<xref rid="app2" ref-type="sec">Figure S2</xref>CV). Thus, FTD-iPSC neuroprogenitors were able to differentiate into functional, excitable neurons.</p><p>Between d24 and d40 of differentiation, transcript levels of <italic>REELIN</italic>, <italic>CTIP2</italic>, <italic>FOXG1</italic>, <italic>FOXP2</italic>, and <italic>TBR1</italic> progressively increased in neural progeny from FTD- and CTRL-iPSC lines. However, on d40, <italic>CTIP2</italic> and <italic>FOXG1</italic> mRNA levels were significantly lower in FTD-iPSC than in CTRL-iPSC progeny (<xref rid="fig1" ref-type="fig">Figures 1</xref>B and <xref rid="app2" ref-type="sec">S2</xref>D). Also, mature TUJ1-positive neurons coexpressed the cortical markers TBR1, FOXP2, and CTIP2. However, compared to CTRL-iPSC and H9-hESC progeny, only a small fraction of FTD-iPSC progeny was positive for TUJ1 (CTRL-iPSCs, 20.7% ± 3.1%; FTD-iPSCs, 4.0% ± 0.69%) (<xref rid="fig1" ref-type="fig">Figures 1</xref>C and 1D). In both CTRL-iPSC and FTD-iPSC progeny, a proportion of undifferentiated NESTIN-positive neuroprogenitors persisted till d40 (<xref rid="fig1" ref-type="fig">Figure 1</xref>E). Thus, using a cortical neuron differentiation protocol, we demonstrate significantly decreased corticogenesis from FTD-iPSCs.</p><p>To test if the neurogenesis defect was specific for cortical neuron generation, FTD-iPSCs and hESCs were differentiated to motor neurons (<xref rid="bib10" ref-type="bibr">Hu and Zhang, 2009</xref>). Immunostaining for the mature motor neuron markers <italic>HB9</italic> and <italic>ISLET1</italic> (<xref rid="fig1" ref-type="fig">Figure 1</xref>F) demonstrated that FTD3-iPSCs generated motor neurons in vitro. Thus, in contrast to what we observed during cortical neuron differentiation, motor neuron generation from FTD-iPSCs was not affected.</p><p>We stained cortical neuron progeny for activated caspase-3 but found no significant differences in the number of apoptotic cells between FTD and CTRL lines (<xref rid="app2" ref-type="sec">Figure S2</xref>E). As <italic>GRN</italic> mutations in humans lead to accumulation of TDP-43-positive inclusions, we performed TDP-43 staining, which did not identify TDP-43 aggregates, and TDP-43 displayed a nuclear staining in all cells (<xref rid="app2" ref-type="sec">Figure S2</xref>F).</p></sec><sec id="sec2.3"><title>Genetic Correction of FTD-iPSCs Restores PGRN Levels</title><p>To study the relationship between PGRN haploinsufficiency and the phenotype observed, we introduced <italic>GRN</italic> cDNA by homologous recombination with zinc finger nucleases (ZFNs) in the <italic>AAVS1</italic> locus of FTD3#6-iPSCs (<xref rid="fig2" ref-type="fig">Figure 2</xref>A). To identify correct targeting and absence of random integrations, we performed genotyping based on PCR and Southern blot analysis (<xref rid="fig2" ref-type="fig">Figures 2</xref>B and 2C). One correctly homozygously targeted clone (#9) derived from the FTD3#6 line (hereinafter referred to as FTD3#6-PGRN) was chosen for complete characterization. As an additional control, we recombined the <italic>GRN</italic> cDNA into the <italic>AAVS1</italic> locus of H9-hESCs (H9-hESC-PGRN) (<xref rid="app2" ref-type="sec">Figure S3</xref>A).</p><p><italic>GRN</italic> transcript levels in FTD3#6-PGRN and H9-hESC-PGRN cells were not significantly different from that in H9-hESCs (<xref rid="fig2" ref-type="fig">Figure 2</xref>D). FTD3#6-PGRN cells expressed the pluripotency markers at levels comparable to that of H9-hESCs (<xref rid="fig2" ref-type="fig">Figures 2</xref>E and 2F) and formed teratomas (<xref rid="fig2" ref-type="fig">Figure 2</xref>G). Genome integrity of FTD3#6-PGRN, assessed by array comparative genomic hybridization, revealed no significant acquired genetic abnormalities after gene editing, compared to the original line.</p></sec><sec id="sec2.4"><title>Genetic Correction of FTD-iPSCs Restores Cortical Neuron Formation</title><p>We differentiated FTD3#6-iPSC, FTD3#6-PGRN, H9-hESC, and H9-hESC-PGRN lines to cortical neurons. Patch clamp recording confirmed the functional maturity of FTD3#6-PGRN and H9-hESC-PGRN neurons (data not shown). Differentiation toward neuroprogenitors until d24 was similar for the FTD3#6, FTD3#6-PGRN, H9-ESC, and H9-PGRN lines, as shown by immunostaining for neuroprogenitor markers (<xref rid="app2" ref-type="sec">Figure S3</xref>B) and quantitative RT-PCR (<xref rid="app2" ref-type="sec">Figure S3</xref>C). However, compared with FTD3#6, d40 FTD3#6-PGRN progeny expressed significantly higher <italic>BLBP</italic>, <italic>FOXG1</italic>, and <italic>CTIP2</italic> transcript levels, which were similar to those in H9-hESC and H9-hESC-PGRN progeny (<xref rid="fig3" ref-type="fig">Figure 3</xref>A; <xref rid="app2" ref-type="sec">Figure S3</xref>C). Immunostaining for TUJ1 and the cortical neuron markers CTIP2, FOXP2, and TBR1 demonstrated that more double-positive cells were present in progeny from the FTD3#6-PGRN line compared to the original isogenic line FTD3#6 (<xref rid="fig3" ref-type="fig">Figure 3</xref>B). Quantification of the number of TUJ1<sup>+</sup> and CTIP2<sup>+</sup> neurons demonstrated that FTD3#6-PGRN iPSC progeny contained significantly more TUJ1<sup>+</sup> and CTIP2<sup>+</sup> neurons compared with FTD3#6 and that genetic correction partially rescued the frequency of neurons generated compared to CTRL-iPSCs (TUJ1: CTRL-iPSC, 23.7% ± 3.8%, FTD3#6-PGRN, 9.4% ± 1.4%, and FTD3#6, 3.2 %± 0.3%; CTIP2: CTRL-iPSC, 14.2% ± 2.2%, FTD3#6-PGRN 6.2, ± 1.9%, and FTD3#6, 1.6% ± 0.5%) (<xref rid="fig3" ref-type="fig">Figure 3</xref>C). Hence, incorporation of <italic>GRN</italic> cDNA in the <italic>AAVS1</italic> locus restored the ability of FTD-iPSCs to differentiate to functional neurons with a cortical fate. As the presence of an extra copy of <italic>GRN</italic> in H9-hESCs did not affect the differentiation potential of H9-hESCs, our studies indicate that deficiency of PGRN is the cause of the in vitro impaired cortical neuron generation from FTD-iPSCs. Incorporation of <italic>GRN</italic> cDNA in the <italic>AAVS1</italic> locus of FTD3#6 iPSCs did not affect motor neuron differentiation (<xref rid="fig3" ref-type="fig">Figures 3</xref>D and 3E).</p></sec><sec id="sec2.5"><title>Genome-wide Transcriptome Analysis of d40 Neuronal Progeny Identifies Increased Wnt Signaling</title><p>To gain insights in possible mechanisms underlying the inefficient cortical neuron generation from FTD-iPSCs, we performed RNA sequencing (RNA-seq) of d40 progeny from FTD3#6, FTD3#6-PGRN, and H9-hESC lines. We identified 2,295 genes differentially expressed between d40 progeny of FTD3#6 and H9-hESCs, whereas only 122 genes were differentially expressed between the FTD3#6-PGRN and the H9-hESC progeny (<xref rid="fig4" ref-type="fig">Figure 4</xref>A).</p><p>RNA-seq data corroborated our observation that cortical neuronal differentiation from FTD-iPSCs is inefficient, as here too, <italic>TUBB3</italic>, <italic>CTIP2</italic>, <italic>FOXG1</italic>, <italic>BLBP</italic>, and <italic>MAP2</italic> were significantly downregulated in FTD3#6, compared to H9-hESC progeny, and restored in FTD3#6-PGRN progeny (<xref rid="fig4" ref-type="fig">Figure 4</xref>B). Although we did not see an increase in apoptotic cells, the RNA-seq data demonstrated that, in line with the findings in the <xref rid="bib1" ref-type="bibr">Almeida et al. (2012)</xref> paper, some components of the MAPK pathway were differentially expressed in the d40 progeny of FTD3#6 cells compared with FTD3#6-PGRN and H9-hESC progeny (<xref rid="app2" ref-type="sec">Figure S4</xref>A).</p><p>Gene ontology analysis using DAVID demonstrated that “neurogenesis,” “generation of neurons,” “neuron development,” “neuron projection development,” and “synaptic transmission” were within the top “Biological Processes” categories significantly enriched in FTD3#6 versus H9-hESC progeny and in FTD3#6-PGRN versus FTD3#6 progeny. Ingenuity pathway analysis identified Wnt/β-catenin signaling as one of the top altered pathways. Among other genes, <italic>WNT2</italic>, <italic>WNT3a</italic>, <italic>WNT5a</italic>, <italic>WNT6</italic>, and <italic>WNT10a; CTNNB1; LEF/TCF</italic> were more highly expressed in FTD3#6 compared to FTD3#6-PGRN and H9-hESC progeny, while <italic>GSK3β</italic>, <italic>APC</italic>, and <italic>PP2A</italic> were expressed significantly less in FTD3#6-iPSC progeny. However, expression of genes from the WNT signaling pathway was similar in FTD3#6-PGRN compared with H9-hESC neural progeny (<xref rid="fig4" ref-type="fig">Figures 4</xref>C and <xref rid="app2" ref-type="sec">S4</xref>B).</p><p>To address whether aberrantly activated WNT signaling was, at least in part, responsible for the defective corticogenesis, we induced cortical neuron differentiation of FTD3#6, FTD3#6-PGRN, and H9-ESC lines in the presence or absence of the WNT inhibitor, IWP2. Western blot analysis demonstrated that IWP2 inhibited active β-catenin levels (<xref rid="fig4" ref-type="fig">Figure 4</xref>D). On d40 of differentiation, transcript levels for <italic>BLBP</italic> and <italic>FOXG1</italic> were significantly higher in FTD3#6 progeny treated with IWP2, dissolved in DMSO, compared to FTD3#6 treated with DMSO and untreated cells (<xref rid="fig4" ref-type="fig">Figures 4</xref>E–4I). Of note, addition of DMSO alone also enhanced expression of <italic>BLBP</italic> and <italic>FOXG1</italic> in FTD3#6 progeny, while IWP2 induced a further significant increase in these transcripts. The increased frequency of neurons in DMSO-treated FTD-iPSC progeny is possibly an indirect effect of DMSO-induced PGRN upregulation, as previously reported (<xref rid="bib19" ref-type="bibr">Ong et al., 2006</xref>). Consistent with this notion, addition of DMSO (and IWP2) to differentiation of FTD3#6-PGRN (<xref rid="fig4" ref-type="fig">Figure 4</xref>EII) or H9-hESC (data not shown) lines did not affect expression of cortical neuron markers. Immunostaining demonstrated that more BLBP- and CTIP2-positive cells were generated when IWP2 was added during the cortical neuron differentiation of FTD3#6 cells (<xref rid="fig4" ref-type="fig">Figure 4</xref>F). Again, this improvement was also visible in the DMSO-treated cells (data not shown).</p></sec></sec><sec sec-type="discussion" id="sec3"><title>Discussion</title><p>We demonstrate that iPSCs generated from FTD patients carrying a <italic>GRN IVS1+5G > C</italic> null mutation poorly differentiate to cortical neurons, while differentiation to motor neurons appears normal. Introduction of the <italic>GRN</italic> cDNA in the <italic>AAVS1</italic> locus of FTD-iPSCs using ZFN-mediated homologous recombination corrected the inefficient cortical neuron generation. Loss of PGRN caused aberrant activation of the WNT signaling pathway during neuronal differentiation, and inhibition of WNT signaling partially rescued the FTD-iPSC corticogenesis defect, further substantiating the notion that the WNT signaling pathway might be a therapeutic target for FTD (<xref rid="bib14" ref-type="bibr">Korade and Mirnics, 2011</xref>).</p><p><xref rid="bib1" ref-type="bibr">Almeida et al. (2012)</xref> previously demonstrated that <italic>GRN</italic><sup>S116X</sup>-iPSCs could be differentiated toward neurons with a similar efficiency as that of control iPSCs but that <italic>GRN</italic><sup>S116X</sup>-iPSCs were more sensitive to stress. We chose to differentiate neuronal progenitors from iPSC lines specifically toward the cortical lineage, as we hypothesized that evaluation of the influence of PGRN haploinsufficiency on cortical neurons, the most affected cell type in FTD, would likely yield the most important insights into the disease. We induced cortical neuron differentiation and demonstrated that generation of TUJ1-positive cells coexpressing cortical markers such as CTIP2, FOXP2, and TBR1 was significantly decreased in FTD-iPSCs. By contrast, Almeida et al., who differentiated iPSCs sequentially as embryoid bodies and neurospheres with additional growth factors to generate a mixture of glutamatergic, GABAergic, and dopaminergic neurons, did not observe decreased cell differentiation. We hypothesize that the differences in the efficiency of neural progeny generation between the two studies might be caused by the specific subtype of neuronal cells we generated. In fact, generation of HB9/TUJ1- and ISLET-1/SMI32-positive motor neurons appeared similar when FTD-iPSC or H9-hESC lines were differentiated to motor neurons.</p><p>The role of PGRN in cortical neurons is currently not well understood. PGRN exerts neurotrophic properties through a yet-unidentified receptor (<xref rid="bib4 bib8 bib25" ref-type="bibr">De Muynck et al., 2013; Gass et al., 2012; Van Damme et al., 2008</xref>). However, a strong link between PGRN and the WNT signaling pathway in neuronal physiology has been established. <xref rid="bib22" ref-type="bibr">Rosen et al. (2011)</xref>, demonstrated that <italic>GRN</italic> knockdown in primary human neurons activated the canonical and noncanonical WNT signaling pathway. They also found similar WNT signaling activation in transcriptome studies of human <italic>GRN</italic> mutant brain. Furthermore, knockdown of <italic>GRN</italic> in a neural progenitor cell line was shown to inhibit the level of phosphorylated GSK3β, resulting in canonical WNT signaling activation and hampered retinoic-acid-mediated neural differentiation in human cells (<xref rid="bib7" ref-type="bibr">Gao et al., 2010</xref>).</p><p>WNT signaling is also important for pre- and postnatal neuronal development. WNT signaling inhibits neuroectoderm and forebrain specification (<xref rid="bib16" ref-type="bibr">Ming and Song, 2011</xref>). Postnatally, WNT regulates proliferation and maturation of neural progenitors in the hippocampus and dentate gyrus (<xref rid="bib17 bib27" ref-type="bibr">Miranda et al., 2012; Wexler et al., 2009</xref>). These studies identify a very important role of WNT signaling during brain development, as well as neurogenesis and neural maturation postnatally.</p><p>Transcriptome studies on cortical neuron progeny from FTD-iPSCs and isogenic genetically corrected FTD-iPSC and CTRL lines revealed that WNT signaling was among the top canonical signaling pathways deregulated by <italic>GRN</italic> haploinsufficiency. This may explain the decrease in mature cortical neurons generated, as WNT signaling was shown to affect proliferation and maturation of cortical neuronal progenitors (<xref rid="bib27" ref-type="bibr">Wexler et al., 2009</xref>). However, we could not identify aberrant activation of the WNT signaling pathway in the <xref rid="bib1" ref-type="bibr">Almeida et al. (2012)</xref> transcriptome studies (<xref rid="app2" ref-type="sec">Figure S4</xref>C), which may, again, be due to differences in neurons generated.</p><p>We also demonstrated that aberrant WNT signaling is, at least partly, responsible for the defective in vitro corticogenesis, based on differentiations wherein we added the WNT inhibitor IWP2, which inhibits WNT processing and secretion. WNT signaling abnormalities, observed in our study, are also seen in brains of 6-week-old <italic>Grn</italic><sup><italic>−/−</italic></sup> mice (<xref rid="bib22" ref-type="bibr">Rosen et al., 2011</xref>) before the occurrence of neuroinflammation or neuronal apoptosis, suggesting that aberrant WNT signaling may be an early event in FTD development. Future studies to assess whether spontaneous apoptosis occurs in FTD-iPSC neurons maintained for longer periods of time, and whether this can be linked to aberrant WNT signaling, will be of interest.</p><p>We found that in vitro corticogenesis is decreased in <italic>GRN</italic><sup>IVS1+5G > C</sup>-iPSCs; however, brain development in <italic>Grn</italic><sup><italic>+/−</italic></sup> and <italic>Grn</italic><sup><italic>−/−</italic></sup> mice and in PGRN haploinsufficient patients appears normal. The reason for the discrepancies in vitro and in vivo might be that excess of WNT signaling during early steps of development can be compensated by other morphogens or cellular interactions, not present in the culture system. However, it is interesting that in patients with FTD, evidence of presymptomatic changes exists (<xref rid="bib15" ref-type="bibr">Li et al., 2007</xref>).</p><p>Neurodegenerative diseases were thought to not be readily recreated in vitro, as they are late-onset diseases. However, several papers have shown that disease phenotypes recapitulated to some extent, using iPSCs (<xref rid="bib20" ref-type="bibr">Qiang et al., 2013</xref>). This suggests that cellular and molecular causes of these diseases of aging may often already be present earlier during life and that additional events ultimately lead to the full manifestation of the symptoms of the disease. In addition, published studies, as well as the study presented here, suggest that signaling events required for neurodevelopment may also play major roles in neurodegeneration and that targeting such pathways—as for instance, the WNT pathway presented in this study—may result in the creation of novel therapeutic approaches for FTD.</p></sec><sec sec-type="methods" id="sec4"><title>Experimental Procedures</title><p>Primary human fibroblast cultures were obtained from skin biopsies of FTD patients after informed consent; control iPSCs were generated from BJ fibroblasts. Human iPSC lines were generated and characterized as previously described (<xref rid="bib24" ref-type="bibr">Takahashi et al., 2007b</xref>). hESC and iPSC lines were differentiated toward cortical or motor neuron progeny as previously described (<xref rid="bib6 bib10" ref-type="bibr">Espuny-Camacho et al., 2013; Hu and Zhang, 2009</xref>). Gene targeting was performed by use of ZFN technology in the <italic>AAVS1</italic> locus of iPSCs.</p><p>For further information, see the <xref rid="app2" ref-type="sec">Supplemental Experimental Procedures</xref>.</p></sec><sec sec-type="acknowledgement" id="sec5"><title>Author Contributions</title><p>C.M.V. and P.V.D. planned the project. S.R., L.O., C.M.V., P.V.D., M.G., and L.D.M. participated in the study design, planning, and interpretation of the experiments. L.D.M. performed the ELISA experiments and sequencing of GRN gene in iPSCs and analyzed RNA-seq data. K.V. generated and characterized the CTRL-iPSC line. W.G. performed the motor neuron differentiations, and L.V.D.B. helped in the interpretation of these results. T.C. provided the <italic>AAVS1</italic> ZFN. S.K. and L.D.M. provided scientific discussions and helped with data interpretation. P.V. and I.E-C. provided a training course for cortical neuron generation and helped with the interpretation of data. B.I.T. and T.V. performed patch-clamp analysis and contributed in writing the manuscript. R.V. provided patient samples. S.R. and C.M.V. wrote the manuscript.</p></sec></body><back><ref-list><title>References</title><ref id="bib1"><element-citation publication-type="journal" id="sref1"><person-group person-group-type="author"><name><surname>Almeida</surname><given-names>S.</given-names></name><name><surname>Zhang</surname><given-names>Z.</given-names></name><name><surname>Coppola</surname><given-names>G.</given-names></name><name><surname>Mao</surname><given-names>W.</given-names></name><name><surname>Futai</surname><given-names>K.</given-names></name><name><surname>Karydas</surname><given-names>A.</given-names></name><name><surname>Geschwind</surname><given-names>M.D.</given-names></name><name><surname>Tartaglia</surname><given-names>M.C.</given-names></name><name><surname>Gao</surname><given-names>F.</given-names></name><name><surname>Gianni</surname><given-names>D.</given-names></name></person-group><article-title>Induced pluripotent stem cell models of progranulin-deficient frontotemporal dementia uncover specific reversible neuronal defects</article-title><source>Cell Reports</source><volume>2</volume><year>2012</year><fpage>789</fpage><lpage>798</lpage><pub-id pub-id-type="pmid">23063362</pub-id></element-citation></ref><ref id="bib2"><element-citation publication-type="journal" id="sref2"><person-group person-group-type="author"><name><surname>Baker</surname><given-names>M.</given-names></name><name><surname>Mackenzie</surname><given-names>I.R.</given-names></name><name><surname>Pickering-Brown</surname><given-names>S.M.</given-names></name><name><surname>Gass</surname><given-names>J.</given-names></name><name><surname>Rademakers</surname><given-names>R.</given-names></name><name><surname>Lindholm</surname><given-names>C.</given-names></name><name><surname>Snowden</surname><given-names>J.</given-names></name><name><surname>Adamson</surname><given-names>J.</given-names></name><name><surname>Sadovnick</surname><given-names>A.D.</given-names></name><name><surname>Rollinson</surname><given-names>S.</given-names></name></person-group><article-title>Mutations in progranulin cause tau-negative frontotemporal dementia linked to chromosome 17</article-title><source>Nature</source><volume>442</volume><year>2006</year><fpage>916</fpage><lpage>919</lpage><pub-id pub-id-type="pmid">16862116</pub-id></element-citation></ref><ref id="bib3"><element-citation publication-type="journal" id="sref3"><person-group person-group-type="author"><name><surname>Cruts</surname><given-names>M.</given-names></name><name><surname>Gijselinck</surname><given-names>I.</given-names></name><name><surname>van der Zee</surname><given-names>J.</given-names></name><name><surname>Engelborghs</surname><given-names>S.</given-names></name><name><surname>Wils</surname><given-names>H.</given-names></name><name><surname>Pirici</surname><given-names>D.</given-names></name><name><surname>Rademakers</surname><given-names>R.</given-names></name><name><surname>Vandenberghe</surname><given-names>R.</given-names></name><name><surname>Dermaut</surname><given-names>B.</given-names></name><name><surname>Martin</surname><given-names>J.J.</given-names></name></person-group><article-title>Null mutations in progranulin cause ubiquitin-positive frontotemporal dementia linked to chromosome 17q21</article-title><source>Nature</source><volume>442</volume><year>2006</year><fpage>920</fpage><lpage>924</lpage><pub-id pub-id-type="pmid">16862115</pub-id></element-citation></ref><ref id="bib4"><element-citation publication-type="journal" id="sref4"><person-group person-group-type="author"><name><surname>De Muynck</surname><given-names>L.</given-names></name><name><surname>Herdewyn</surname><given-names>S.</given-names></name><name><surname>Beel</surname><given-names>S.</given-names></name><name><surname>Scheveneels</surname><given-names>W.</given-names></name><name><surname>Van Den Bosch</surname><given-names>L.</given-names></name><name><surname>Robberecht</surname><given-names>W.</given-names></name><name><surname>Van Damme</surname><given-names>P.</given-names></name></person-group><article-title>The neurotrophic properties of progranulin depend on the granulin E domain but do not require sortilin binding</article-title><source>Neurobiol. Aging</source><volume>34</volume><year>2013</year><fpage>2541</fpage><lpage>2547</lpage><pub-id pub-id-type="pmid">23706646</pub-id></element-citation></ref><ref id="bib5"><element-citation publication-type="journal" id="sref5"><person-group person-group-type="author"><name><surname>DeJesus-Hernandez</surname><given-names>M.</given-names></name><name><surname>Mackenzie</surname><given-names>I.R.</given-names></name><name><surname>Boeve</surname><given-names>B.F.</given-names></name><name><surname>Boxer</surname><given-names>A.L.</given-names></name><name><surname>Baker</surname><given-names>M.</given-names></name><name><surname>Rutherford</surname><given-names>N.J.</given-names></name><name><surname>Nicholson</surname><given-names>A.M.</given-names></name><name><surname>Finch</surname><given-names>N.A.</given-names></name><name><surname>Flynn</surname><given-names>H.</given-names></name><name><surname>Adamson</surname><given-names>J.</given-names></name></person-group><article-title>Expanded GGGGCC hexanucleotide repeat in noncoding region of C9ORF72 causes chromosome 9p-linked FTD and ALS</article-title><source>Neuron</source><volume>72</volume><year>2011</year><fpage>245</fpage><lpage>256</lpage><pub-id pub-id-type="pmid">21944778</pub-id></element-citation></ref><ref id="bib6"><element-citation publication-type="journal" id="sref6"><person-group person-group-type="author"><name><surname>Espuny-Camacho</surname><given-names>I.</given-names></name><name><surname>Michelsen</surname><given-names>K.A.</given-names></name><name><surname>Gall</surname><given-names>D.</given-names></name><name><surname>Linaro</surname><given-names>D.</given-names></name><name><surname>Hasche</surname><given-names>A.</given-names></name><name><surname>Bonnefont</surname><given-names>J.</given-names></name><name><surname>Bali</surname><given-names>C.</given-names></name><name><surname>Orduz</surname><given-names>D.</given-names></name><name><surname>Bilheu</surname><given-names>A.</given-names></name><name><surname>Herpoel</surname><given-names>A.</given-names></name></person-group><article-title>Pyramidal neurons derived from human pluripotent stem cells integrate efficiently into mouse brain circuits in vivo</article-title><source>Neuron</source><volume>77</volume><year>2013</year><fpage>440</fpage><lpage>456</lpage><pub-id pub-id-type="pmid">23395372</pub-id></element-citation></ref><ref id="bib7"><element-citation publication-type="journal" id="sref7"><person-group person-group-type="author"><name><surname>Gao</surname><given-names>X.</given-names></name><name><surname>Joselin</surname><given-names>A.P.</given-names></name><name><surname>Wang</surname><given-names>L.</given-names></name><name><surname>Kar</surname><given-names>A.</given-names></name><name><surname>Ray</surname><given-names>P.</given-names></name><name><surname>Bateman</surname><given-names>A.</given-names></name><name><surname>Goate</surname><given-names>A.M.</given-names></name><name><surname>Wu</surname><given-names>J.Y.</given-names></name></person-group><article-title>Progranulin promotes neurite outgrowth and neuronal differentiation by regulating GSK-3β</article-title><source>Protein Cell.</source><volume>1</volume><year>2010</year><fpage>552</fpage><lpage>562</lpage><pub-id pub-id-type="pmid">21204008</pub-id></element-citation></ref><ref id="bib8"><element-citation publication-type="journal" id="sref8"><person-group person-group-type="author"><name><surname>Gass</surname><given-names>J.</given-names></name><name><surname>Lee</surname><given-names>W.C.</given-names></name><name><surname>Cook</surname><given-names>C.</given-names></name><name><surname>Finch</surname><given-names>N.</given-names></name><name><surname>Stetler</surname><given-names>C.</given-names></name><name><surname>Jansen-West</surname><given-names>K.</given-names></name><name><surname>Lewis</surname><given-names>J.</given-names></name><name><surname>Link</surname><given-names>C.D.</given-names></name><name><surname>Rademakers</surname><given-names>R.</given-names></name><name><surname>Nykjær</surname><given-names>A.</given-names></name><name><surname>Petrucelli</surname><given-names>L.</given-names></name></person-group><article-title>Progranulin regulates neuronal outgrowth independent of sortilin</article-title><source>Mol. Neurodegener.</source><volume>7</volume><year>2012</year><fpage>33</fpage><pub-id pub-id-type="pmid">22781549</pub-id></element-citation></ref><ref id="bib9"><element-citation publication-type="journal" id="sref9"><person-group person-group-type="author"><name><surname>Goldman</surname><given-names>J.S.</given-names></name><name><surname>Farmer</surname><given-names>J.M.</given-names></name><name><surname>Wood</surname><given-names>E.M.</given-names></name><name><surname>Johnson</surname><given-names>J.K.</given-names></name><name><surname>Boxer</surname><given-names>A.</given-names></name><name><surname>Neuhaus</surname><given-names>J.</given-names></name><name><surname>Lomen-Hoerth</surname><given-names>C.</given-names></name><name><surname>Wilhelmsen</surname><given-names>K.C.</given-names></name><name><surname>Lee</surname><given-names>V.M.</given-names></name><name><surname>Grossman</surname><given-names>M.</given-names></name><name><surname>Miller</surname><given-names>B.L.</given-names></name></person-group><article-title>Comparison of family histories in FTLD subtypes and related tauopathies</article-title><source>Neurology</source><volume>65</volume><year>2005</year><fpage>1817</fpage><lpage>1819</lpage><pub-id pub-id-type="pmid">16344531</pub-id></element-citation></ref><ref id="bib10"><element-citation publication-type="journal" id="sref10"><person-group person-group-type="author"><name><surname>Hu</surname><given-names>B.Y.</given-names></name><name><surname>Zhang</surname><given-names>S.C.</given-names></name></person-group><article-title>Differentiation of spinal motor neurons from pluripotent human stem cells</article-title><source>Nat. Protoc.</source><volume>4</volume><year>2009</year><fpage>1295</fpage><lpage>1304</lpage><pub-id pub-id-type="pmid">19696748</pub-id></element-citation></ref><ref id="bib11"><element-citation publication-type="journal" id="sref11"><person-group person-group-type="author"><name><surname>Hutton</surname><given-names>M.</given-names></name><name><surname>Lendon</surname><given-names>C.L.</given-names></name><name><surname>Rizzu</surname><given-names>P.</given-names></name><name><surname>Baker</surname><given-names>M.</given-names></name><name><surname>Froelich</surname><given-names>S.</given-names></name><name><surname>Houlden</surname><given-names>H.</given-names></name><name><surname>Pickering-Brown</surname><given-names>S.</given-names></name><name><surname>Chakraverty</surname><given-names>S.</given-names></name><name><surname>Isaacs</surname><given-names>A.</given-names></name><name><surname>Grover</surname><given-names>A.</given-names></name></person-group><article-title>Association of missense and 5′-splice-site mutations in tau with the inherited dementia FTDP-17</article-title><source>Nature</source><volume>393</volume><year>1998</year><fpage>702</fpage><lpage>705</lpage><pub-id pub-id-type="pmid">9641683</pub-id></element-citation></ref><ref id="bib12"><element-citation publication-type="journal" id="sref12"><person-group person-group-type="author"><name><surname>Kayasuga</surname><given-names>Y.</given-names></name><name><surname>Chiba</surname><given-names>S.</given-names></name><name><surname>Suzuki</surname><given-names>M.</given-names></name><name><surname>Kikusui</surname><given-names>T.</given-names></name><name><surname>Matsuwaki</surname><given-names>T.</given-names></name><name><surname>Yamanouchi</surname><given-names>K.</given-names></name><name><surname>Kotaki</surname><given-names>H.</given-names></name><name><surname>Horai</surname><given-names>R.</given-names></name><name><surname>Iwakura</surname><given-names>Y.</given-names></name><name><surname>Nishihara</surname><given-names>M.</given-names></name></person-group><article-title>Alteration of behavioural phenotype in mice by targeted disruption of the progranulin gene</article-title><source>Behav. Brain Res.</source><volume>185</volume><year>2007</year><fpage>110</fpage><lpage>118</lpage><pub-id pub-id-type="pmid">17764761</pub-id></element-citation></ref><ref id="bib13"><element-citation publication-type="journal" id="sref13"><person-group person-group-type="author"><name><surname>Kim</surname><given-names>C.</given-names></name></person-group><article-title>Disease modeling and cell based therapy with iPSC: future therapeutic option with fast and safe application</article-title><source>Blood Res.</source><volume>49</volume><year>2014</year><fpage>7</fpage><lpage>14</lpage><pub-id pub-id-type="pmid">24724061</pub-id></element-citation></ref><ref id="bib14"><element-citation publication-type="journal" id="sref14"><person-group person-group-type="author"><name><surname>Korade</surname><given-names>Z.</given-names></name><name><surname>Mirnics</surname><given-names>K.</given-names></name></person-group><article-title>Wnt signaling as a potential therapeutic target for frontotemporal dementia</article-title><source>Neuron</source><volume>71</volume><year>2011</year><fpage>955</fpage><lpage>957</lpage><pub-id pub-id-type="pmid">21943593</pub-id></element-citation></ref><ref id="bib15"><element-citation publication-type="journal" id="sref15"><person-group person-group-type="author"><name><surname>Li</surname><given-names>H.L.</given-names></name><name><surname>Wang</surname><given-names>H.H.</given-names></name><name><surname>Liu</surname><given-names>S.J.</given-names></name><name><surname>Deng</surname><given-names>Y.Q.</given-names></name><name><surname>Zhang</surname><given-names>Y.J.</given-names></name><name><surname>Tian</surname><given-names>Q.</given-names></name><name><surname>Wang</surname><given-names>X.C.</given-names></name><name><surname>Chen</surname><given-names>X.Q.</given-names></name><name><surname>Yang</surname><given-names>Y.</given-names></name><name><surname>Zhang</surname><given-names>J.Y.</given-names></name></person-group><article-title>Phosphorylation of tau antagonizes apoptosis by stabilizing beta-catenin, a mechanism involved in Alzheimer’s neurodegeneration</article-title><source>Proc. Natl. Acad. Sci. USA</source><volume>104</volume><year>2007</year><fpage>3591</fpage><lpage>3596</lpage><pub-id pub-id-type="pmid">17360687</pub-id></element-citation></ref><ref id="bib16"><element-citation publication-type="journal" id="sref16"><person-group person-group-type="author"><name><surname>Ming</surname><given-names>G.L.</given-names></name><name><surname>Song</surname><given-names>H.</given-names></name></person-group><article-title>Adult neurogenesis in the mammalian brain: significant answers and significant questions</article-title><source>Neuron</source><volume>70</volume><year>2011</year><fpage>687</fpage><lpage>702</lpage><pub-id pub-id-type="pmid">21609825</pub-id></element-citation></ref><ref id="bib17"><element-citation publication-type="journal" id="sref17"><person-group person-group-type="author"><name><surname>Miranda</surname><given-names>C.J.</given-names></name><name><surname>Braun</surname><given-names>L.</given-names></name><name><surname>Jiang</surname><given-names>Y.</given-names></name><name><surname>Hester</surname><given-names>M.E.</given-names></name><name><surname>Zhang</surname><given-names>L.</given-names></name><name><surname>Riolo</surname><given-names>M.</given-names></name><name><surname>Wang</surname><given-names>H.</given-names></name><name><surname>Rao</surname><given-names>M.</given-names></name><name><surname>Altura</surname><given-names>R.A.</given-names></name><name><surname>Kaspar</surname><given-names>B.K.</given-names></name></person-group><article-title>Aging brain microenvironment decreases hippocampal neurogenesis through Wnt-mediated survivin signaling</article-title><source>Aging Cell</source><volume>11</volume><year>2012</year><fpage>542</fpage><lpage>552</lpage><pub-id pub-id-type="pmid">22404871</pub-id></element-citation></ref><ref id="bib18"><element-citation publication-type="journal" id="sref18"><person-group person-group-type="author"><name><surname>Neary</surname><given-names>D.</given-names></name><name><surname>Snowden</surname><given-names>J.</given-names></name><name><surname>Mann</surname><given-names>D.</given-names></name></person-group><article-title>Frontotemporal dementia</article-title><source>Lancet Neurol.</source><volume>4</volume><year>2005</year><fpage>771</fpage><lpage>780</lpage><pub-id pub-id-type="pmid">16239184</pub-id></element-citation></ref><ref id="bib19"><element-citation publication-type="journal" id="sref19"><person-group person-group-type="author"><name><surname>Ong</surname><given-names>C.H.</given-names></name><name><surname>He</surname><given-names>Z.</given-names></name><name><surname>Kriazhev</surname><given-names>L.</given-names></name><name><surname>Shan</surname><given-names>X.</given-names></name><name><surname>Palfree</surname><given-names>R.G.</given-names></name><name><surname>Bateman</surname><given-names>A.</given-names></name></person-group><article-title>Regulation of progranulin expression in myeloid cells</article-title><source>Am. J. Physiol. Regul. Integr. Comp. Physiol.</source><volume>291</volume><year>2006</year><fpage>R1602</fpage><lpage>R1612</lpage><pub-id pub-id-type="pmid">16873554</pub-id></element-citation></ref><ref id="bib20"><element-citation publication-type="journal" id="sref20"><person-group person-group-type="author"><name><surname>Qiang</surname><given-names>L.</given-names></name><name><surname>Fujita</surname><given-names>R.</given-names></name><name><surname>Abeliovich</surname><given-names>A.</given-names></name></person-group><article-title>Remodeling neurodegeneration: somatic cell reprogramming-based models of adult neurological disorders</article-title><source>Neuron</source><volume>78</volume><year>2013</year><fpage>957</fpage><lpage>969</lpage><pub-id pub-id-type="pmid">23791192</pub-id></element-citation></ref><ref id="bib21"><element-citation publication-type="journal" id="sref21"><person-group person-group-type="author"><name><surname>Renton</surname><given-names>A.E.</given-names></name><name><surname>Majounie</surname><given-names>E.</given-names></name><name><surname>Waite</surname><given-names>A.</given-names></name><name><surname>Simón-Sánchez</surname><given-names>J.</given-names></name><name><surname>Rollinson</surname><given-names>S.</given-names></name><name><surname>Gibbs</surname><given-names>J.R.</given-names></name><name><surname>Schymick</surname><given-names>J.C.</given-names></name><name><surname>Laaksovirta</surname><given-names>H.</given-names></name><name><surname>van Swieten</surname><given-names>J.C.</given-names></name><name><surname>Myllykangas</surname><given-names>L.</given-names></name><collab>ITALSGEN Consortium</collab></person-group><article-title>A hexanucleotide repeat expansion in C9ORF72 is the cause of chromosome 9p21-linked ALS-FTD</article-title><source>Neuron</source><volume>72</volume><year>2011</year><fpage>257</fpage><lpage>268</lpage><pub-id pub-id-type="pmid">21944779</pub-id></element-citation></ref><ref id="bib22"><element-citation publication-type="journal" id="sref22"><person-group person-group-type="author"><name><surname>Rosen</surname><given-names>E.Y.</given-names></name><name><surname>Wexler</surname><given-names>E.M.</given-names></name><name><surname>Versano</surname><given-names>R.</given-names></name><name><surname>Coppola</surname><given-names>G.</given-names></name><name><surname>Gao</surname><given-names>F.</given-names></name><name><surname>Winden</surname><given-names>K.D.</given-names></name><name><surname>Oldham</surname><given-names>M.C.</given-names></name><name><surname>Martens</surname><given-names>L.H.</given-names></name><name><surname>Zhou</surname><given-names>P.</given-names></name><name><surname>Farese</surname><given-names>R.V.</given-names><suffix>Jr.</suffix></name><name><surname>Geschwind</surname><given-names>D.H.</given-names></name></person-group><article-title>Functional genomic analyses identify pathways dysregulated by progranulin deficiency, implicating Wnt signaling</article-title><source>Neuron</source><volume>71</volume><year>2011</year><fpage>1030</fpage><lpage>1042</lpage><pub-id pub-id-type="pmid">21943601</pub-id></element-citation></ref><ref id="bib23"><element-citation publication-type="journal" id="sref23"><person-group person-group-type="author"><name><surname>Takahashi</surname><given-names>K.</given-names></name><name><surname>Okita</surname><given-names>K.</given-names></name><name><surname>Nakagawa</surname><given-names>M.</given-names></name><name><surname>Yamanaka</surname><given-names>S.</given-names></name></person-group><article-title>Induction of pluripotent stem cells from fibroblast cultures</article-title><source>Nat. Protoc.</source><volume>2</volume><year>2007</year><fpage>3081</fpage><lpage>3089</lpage><pub-id pub-id-type="pmid">18079707</pub-id></element-citation></ref><ref id="bib24"><element-citation publication-type="journal" id="sref24"><person-group person-group-type="author"><name><surname>Takahashi</surname><given-names>K.</given-names></name><name><surname>Tanabe</surname><given-names>K.</given-names></name><name><surname>Ohnuki</surname><given-names>M.</given-names></name><name><surname>Narita</surname><given-names>M.</given-names></name><name><surname>Ichisaka</surname><given-names>T.</given-names></name><name><surname>Tomoda</surname><given-names>K.</given-names></name><name><surname>Yamanaka</surname><given-names>S.</given-names></name></person-group><article-title>Induction of pluripotent stem cells from adult human fibroblasts by defined factors</article-title><source>Cell</source><volume>131</volume><year>2007</year><fpage>861</fpage><lpage>872</lpage><pub-id pub-id-type="pmid">18035408</pub-id></element-citation></ref><ref id="bib25"><element-citation publication-type="journal" id="sref25"><person-group person-group-type="author"><name><surname>Van Damme</surname><given-names>P.</given-names></name><name><surname>Van Hoecke</surname><given-names>A.</given-names></name><name><surname>Lambrechts</surname><given-names>D.</given-names></name><name><surname>Vanacker</surname><given-names>P.</given-names></name><name><surname>Bogaert</surname><given-names>E.</given-names></name><name><surname>van Swieten</surname><given-names>J.</given-names></name><name><surname>Carmeliet</surname><given-names>P.</given-names></name><name><surname>Van Den Bosch</surname><given-names>L.</given-names></name><name><surname>Robberecht</surname><given-names>W.</given-names></name></person-group><article-title>Progranulin functions as a neurotrophic factor to regulate neurite outgrowth and enhance neuronal survival</article-title><source>J. Cell Biol.</source><volume>181</volume><year>2008</year><fpage>37</fpage><lpage>41</lpage><pub-id pub-id-type="pmid">18378771</pub-id></element-citation></ref><ref id="bib26"><element-citation publication-type="journal" id="sref26"><person-group person-group-type="author"><name><surname>van Swieten</surname><given-names>J.C.</given-names></name><name><surname>Heutink</surname><given-names>P.</given-names></name></person-group><article-title>Mutations in progranulin (GRN) within the spectrum of clinical and pathological phenotypes of frontotemporal dementia</article-title><source>Lancet Neurol.</source><volume>7</volume><year>2008</year><fpage>965</fpage><lpage>974</lpage><pub-id pub-id-type="pmid">18771956</pub-id></element-citation></ref><ref id="bib27"><element-citation publication-type="journal" id="sref27"><person-group person-group-type="author"><name><surname>Wexler</surname><given-names>E.M.</given-names></name><name><surname>Paucer</surname><given-names>A.</given-names></name><name><surname>Kornblum</surname><given-names>H.I.</given-names></name><name><surname>Palmer</surname><given-names>T.D.</given-names></name><name><surname>Geschwind</surname><given-names>D.H.</given-names></name></person-group><article-title>Endogenous Wnt signaling maintains neural progenitor cell potency</article-title><source>Stem Cells</source><volume>27</volume><year>2009</year><fpage>1130</fpage><lpage>1141</lpage><pub-id pub-id-type="pmid">19418460</pub-id></element-citation></ref></ref-list><sec id="app2" sec-type="supplementary-material"><title>Supplemental Information</title><p><supplementary-material content-type="local-data" id="mmc1"><caption><title>Document S1. Supplemental Experimental Procedures and Figures S1–S4</title></caption><media xlink:href="mmc1.pdf"></media></supplementary-material><supplementary-material content-type="local-data" id="mmc2"><caption><title>Document S2. Article plus Supplemental Information</title></caption><media xlink:href="mmc2.pdf"></media></supplementary-material></p></sec><ack id="ack0010"><title>Acknowledgments</title><p>We thank the families of the patients who participated in this study. We also thank Mr. Javed Manesia and Dr. Maria Elena Pistoni for their critical help in the review of the data, Prof. Tanya Roskams for the analysis of the teratomas, and Dr. Anja Hasche for her expertise in WNT signaling. T.C. is a consultant for CRISPR Therapeutics. The work was supported by FWO grants G.0832 (to C.M.V. and P.V.) and G.0667.07 (to C.M.V.), by grants from KU Leuven (EIW-B4855-EF/05/11, ETH-C1900-PF, and EME-C2161-GOA/11/012 to C.M.V.) and by a BELSPO-IUAP-DEVREPAIR grant to C.M.V. This project was supported by a grant from the KU Leuven (GOA/11/014), by the Stichting Alzheimer Onderzoek (SAO), and by the Interuniversity Attraction Poles (IUAP) program P7/16 of the Belgian Federal Science Policy Office. P.V.D. is supported by the Belgian ALS liga. P.V.D. and R.V.D.B. are senior clinical investigators of FWO-Vlaanderen.</p></ack><fn-group><fn id="d32e227"><p>This is an open access article under the CC BY-NC-ND license (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc-nd/3.0/" id="ccintref0005">http://creativecommons.org/licenses/by-nc-nd/3.0/</ext-link>).</p></fn></fn-group></back><floats-group><fig id="fig1"><label>Figure 1</label><caption><p>Generation of Cortical Neurons from FTD-iPSCs and CTRL Lines</p><p>(A) <italic>GRN</italic> levels in FTD-iPSC lines compared to those in control CTRL-iPSC lines (n = 3 independent experiments). Error bars indicate mean ± SEM. <sup>∗</sup>p < 0.05, t test.</p><p>(B) <italic>CTIP</italic> and <italic>FOXG1</italic> expression in d40 progeny of FTD-iPSC and CTRL lines (n = 3 independent experiments). Error bars indicate mean ± SEM. <sup>∗</sup>p < 0.05, t test.</p><p>(C) Immunostaining for TUJ1 and the cortical markers TBR1, CTIP2, and FOXP2. FTD1#1-iPSC and CTRL-iPSC progeny at d40 are shown. Scale bar, 100 μm.</p><p>(D) Enumeration of TUJ1-positive cells in FTD-iPSC and CTRL-iPSC d40 progeny. FTD1#1 and FTD3#6 (FTD) lines and CTRL-iPSC and H9-hESC (CTRL) lines are shown (n = 3 independent experiments per line. Error bars indicate mean ± SEM. <sup>∗</sup>p < 0.05, t test.</p><p>(E) Immunostaining on d40 progeny for Nestin (n = 3). Scale bar, 100 μm.</p><p>(F) Immunostaining of FTD3#6 on d40 of differentiation to motorneurons for TUJ1, SMI32, HB9, and ISLET1 (n = 2). Scale bar, 100 μm.</p><p>See also <xref rid="app2" ref-type="sec">Figures S1</xref> and <xref rid="app2" ref-type="sec">S2</xref>.</p></caption><graphic xlink:href="gr1"></graphic></fig><fig id="fig2"><label>Figure 2</label><caption><p>Gene Targeting Using ZFNs</p><p>(A) Schematic representation of <italic>GRN</italic> gene targeting in the <italic>AAVS1</italic> locus. HA, homology arm; CAGGS, chicken β-actin promoter; HYG<sup>R</sup>, hygromycin resistance-thymidine kinase fusion gene. Blue arrows, primers designed for junction assay (JA) PCR at the 5′ of the construct; red arrows, PCR for JA at the 3′. PURO<sup>R</sup>, puromycin resistance.</p><p>(B) JA-PCR for several clones; the clone number 9 was selected. <bold>+</bold>, positive control for 3′ JA (engineered H9-hESC line created by L.O. [unpublished data]); <bold>−</bold>, negative control (FTD-iPSC not targeted).</p><p>(C) Southern blot of FTD3#6 and FTD3#6-PGRN lines.</p><p>(D) <italic>GRN</italic> expression in FTD3#6, H9-hESC, FTD3#6-PGRN, and H9-PGRN lines (NS, not significant, p = 1.6, t test; n = 3 different passages). Error bars indicate mean ± SEM. <sup>#</sup>p = 0.07; <sup>∗</sup>p < 0.05, t test.</p><p>(E) <italic>NANOG</italic>, <italic>OCT4</italic>, and <italic>DPPA4</italic> expression in FTD3#6, FTD3#6-PGRN, and H9-hESC lines (NS, not significant, p = 0.26, t test; n = 3 different passages). Error bars indicate mean ± SEM.</p><p>(F) Immunostaining of FTD3#6-PGRN for OCT4, SSEA4, and TRA1-60 (n = 3). Scale bar, 100 μm.</p><p>(G) FTD3#6-PGRN-derived teratoma with presence of derivatives of all three germ layers. Scale bar, 500 μm.</p><p>See also <xref rid="app2" ref-type="sec">Figure S3</xref>.</p></caption><graphic xlink:href="gr2"></graphic></fig><fig id="fig3"><label>Figure 3</label><caption><p>Rescue of Corticogenesis in FTD3#6-PGRN Cells</p><p>(A) <italic>BLBP</italic>, <italic>FOXG1</italic>, and <italic>CTIP2</italic> expression in FTD3#6-PGRN, FTD3#6-iPSC, H9-hESC, and H9-PGRN lines (n = 3 independent experiments). NS, not significant; p = 0.28, p = 0.23, p = 0.2 for top, middle, and bottom panels, respectively, t test. Error bars indicate mean ± SEM. <sup>∗</sup>p < 0.05, t test.</p><p>(B) Immunostaining of FTD3#6 and FTD3#6-PGRN for TUJ1 and CTIP2, FOXP2, and TBR1 (n = 3). Scale bars, 100 μm.</p><p>(C) Enumeration of TUJ1-positive (I) and CTIP2-positive cells (II) in d40 progeny from FTD3#6-PGRN iPSCs compared to FTD3#6 and CTRL-iPSC lines (n = 3 independent experiments per line). Error bars indicate mean ± SEM. <sup>∗</sup>p < 0.05, t test.</p><p>(D) Immunostaining of FTD3#6-PGRN for TUJ1 and HB9 (n = 2). Scale bar, 100 μm.</p><p>(E) Transcript levels of motorneuron precursor and mature markers (n = 2 independent experiments). Error bars indicate mean ± SEM.</p><p>See also <xref rid="app2" ref-type="sec">Figure S3</xref>.</p></caption><graphic xlink:href="gr3"></graphic></fig><fig id="fig4"><label>Figure 4</label><caption><p>Genome-wide Transcriptome Analysis of d40 Neuronal Progeny</p><p>(A) Table with the significantly differentially expressed genes (using Benjamini-Hochberg-corrected p values).</p><p>(B) Represents the log fold change of gene transcripts of <italic>TUBB3</italic>, <italic>TBR1</italic>, <italic>FOXP2</italic>, <italic>CTIP2</italic>, <italic>FOXG1</italic>, <italic>BLBP</italic>, and <italic>MAP2</italic> in d40 neural progeny of the FTD3#6, H9-hESC, and FTD3#6-PGRN lines from RNA-seq studies.</p><p>(C) Wnt signaling pathway, overlaid with the differentially expressed genes (log<sub>2</sub> ratio >1 or <−1) of FTD3#6 versus H9-hESC line comparison. Upregulated genes are in green, and downregulated genes are in red.</p><p>(D) Western blot for active and total β-catenin on H9-hESCs at d24 treated with DMSO or DMSO-IWP2 (IWP2). β-actin, internal control.</p><p>(E) Panel I: Relative expression of <italic>BLBP</italic> and <italic>FOXG1</italic> transcripts in FTD3#6 treated with or without DMSO and IWP2 (n = 3 independent experiments). Error bars indicate mean ± SEM; <sup>∗</sup>p < 0.05, t test. Panel II: <italic>BLBP</italic> and <italic>FOXG1</italic> transcripts in FTD3#6-iPSC treated with DMSO or DMSO-IWP2 (IWP2). n = 3 independent experiments. Error bars indicate mean ± SEM. <sup>∗</sup>p < 0.05, t test.</p><p>(F) Immunostaining on FTD3#6 d40 progeny treated with DMSO-IWP2 (IWP2) for NESTIN and BLBP or TUJ1 and CTIP2 (n = 3). Scale bars, 100 μm.</p><p>See also <xref rid="app2" ref-type="sec">Figure S4</xref>.</p></caption><graphic xlink:href="gr4"></graphic></fig></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000261 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000261 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Pmc
|étape= Corpus
|type= RBID
|clé= PMC:4297877
|texte= Restoration of Progranulin Expression Rescues Cortical Neuron Generation in an Induced Pluripotent Stem Cell Model of Frontotemporal Dementia
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:25556567" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a OpenAccessBelV2
| This area was generated with Dilib version V0.6.25. |  |