Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine
Identifieur interne : 000195 ( Pmc/Checkpoint ); précédent : 000194; suivant : 000196Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine
Auteurs : Michael Vogeser [Allemagne] ; Maria Shipkova [Allemagne] ; Raül Rigo-Bonnin [Espagne] ; Pierre Wallemacq [Belgique] ; Matthias Orth [Allemagne] ; Monika Widmann [Allemagne] ; Alain G. Verstraete [Belgique]Source :
- Therapeutic Drug Monitoring [ 0163-4356 ] ; 2014.
Abstract
Supplemental Digital Content is Available in the Text.
Url:
DOI: 10.1097/FTD.0000000000000068
PubMed: 24646730
PubMed Central: 4218761
Affiliations:
- Allemagne, Belgique, Espagne
- Bade-Wurtemberg, Bavière, Catalogne, District de Haute-Bavière, District de Karlsruhe, District de Stuttgart, Région de Bruxelles-Capitale
- Barcelone, Bruxelles, Mannheim, Munich, Stuttgart
- Université Louis-et-Maximilien de Munich
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4218761Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine</title>
<author><name sortKey="Vogeser, Michael" sort="Vogeser, Michael" uniqKey="Vogeser M" first="Michael" last="Vogeser">Michael Vogeser</name>
<affiliation wicri:level="4"><nlm:aff id="aff1">Hospital of the University of Munich, Institute of Laboratory Medicine;</nlm:aff>
<orgName type="university">Université Louis-et-Maximilien de Munich</orgName>
<country>Allemagne</country>
<placeName><settlement type="city">Munich</settlement>
<region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shipkova, Maria" sort="Shipkova, Maria" uniqKey="Shipkova M" first="Maria" last="Shipkova">Maria Shipkova</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Central Institute of Clinical Chemistry and Laboratory Medicine, Klinikum-Stuttgart, Germany;</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Central Institute of Clinical Chemistry and Laboratory Medicine, Klinikum-Stuttgart</wicri:regionArea>
<wicri:noRegion>Klinikum-Stuttgart</wicri:noRegion>
<wicri:noRegion>Klinikum-Stuttgart</wicri:noRegion>
<wicri:noRegion>Klinikum-Stuttgart</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rigo Bonnin, Raul" sort="Rigo Bonnin, Raul" uniqKey="Rigo Bonnin R" first="Raül" last="Rigo-Bonnin">Raül Rigo-Bonnin</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Laboratori Clínic Department, Hospital Universitari de Bellvitge, Barcelona, Spain;</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Laboratori Clínic Department, Hospital Universitari de Bellvitge, Barcelona</wicri:regionArea>
<placeName><settlement type="city">Barcelone</settlement>
<region nuts="2" type="region">Catalogne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wallemacq, Pierre" sort="Wallemacq, Pierre" uniqKey="Wallemacq P" first="Pierre" last="Wallemacq">Pierre Wallemacq</name>
<affiliation wicri:level="3"><nlm:aff id="aff4">Cliniques Universitaires St. Luc, Louvain Toxicology and Applied Pharmacology, Université Catholique de Louvain, Brussels, Belgium;</nlm:aff>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Cliniques Universitaires St. Luc, Louvain Toxicology and Applied Pharmacology, Université Catholique de Louvain, Brussels</wicri:regionArea>
<placeName><settlement type="city">Bruxelles</settlement>
<region nuts="2">Région de Bruxelles-Capitale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Orth, Matthias" sort="Orth, Matthias" uniqKey="Orth M" first="Matthias" last="Orth">Matthias Orth</name>
<affiliation wicri:level="3"><nlm:aff id="aff5">Institue for Laboratory Medicine, Marienhospital, Stuttgart;</nlm:aff>
<country>Allemagne</country>
<placeName><settlement type="city">Stuttgart</settlement>
<region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Stuttgart</region>
</placeName>
<wicri:orgArea>Institue for Laboratory Medicine, Marienhospital</wicri:orgArea>
</affiliation>
</author>
<author><name sortKey="Widmann, Monika" sort="Widmann, Monika" uniqKey="Widmann M" first="Monika" last="Widmann">Monika Widmann</name>
<affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="aff6">Roche Diagnostics GmbH, Mannheim, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Roche Diagnostics GmbH, Mannheim</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Karlsruhe</region>
<settlement type="city">Mannheim</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Verstraete, Alain G" sort="Verstraete, Alain G" uniqKey="Verstraete A" first="Alain G." last="Verstraete">Alain G. Verstraete</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">Department of Laboratory Medicine, Ghent University and Ghent University Hospital, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea>Department of Laboratory Medicine, Ghent University and Ghent University Hospital</wicri:regionArea>
<wicri:noRegion>Ghent University and Ghent University Hospital</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24646730</idno>
<idno type="pmc">4218761</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4218761</idno>
<idno type="RBID">PMC:4218761</idno>
<idno type="doi">10.1097/FTD.0000000000000068</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000434</idno>
<idno type="wicri:Area/Pmc/Curation">000434</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000195</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine</title>
<author><name sortKey="Vogeser, Michael" sort="Vogeser, Michael" uniqKey="Vogeser M" first="Michael" last="Vogeser">Michael Vogeser</name>
<affiliation wicri:level="4"><nlm:aff id="aff1">Hospital of the University of Munich, Institute of Laboratory Medicine;</nlm:aff>
<orgName type="university">Université Louis-et-Maximilien de Munich</orgName>
<country>Allemagne</country>
<placeName><settlement type="city">Munich</settlement>
<region type="land" nuts="1">Bavière</region>
<region type="district" nuts="2">District de Haute-Bavière</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shipkova, Maria" sort="Shipkova, Maria" uniqKey="Shipkova M" first="Maria" last="Shipkova">Maria Shipkova</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Central Institute of Clinical Chemistry and Laboratory Medicine, Klinikum-Stuttgart, Germany;</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Central Institute of Clinical Chemistry and Laboratory Medicine, Klinikum-Stuttgart</wicri:regionArea>
<wicri:noRegion>Klinikum-Stuttgart</wicri:noRegion>
<wicri:noRegion>Klinikum-Stuttgart</wicri:noRegion>
<wicri:noRegion>Klinikum-Stuttgart</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Rigo Bonnin, Raul" sort="Rigo Bonnin, Raul" uniqKey="Rigo Bonnin R" first="Raül" last="Rigo-Bonnin">Raül Rigo-Bonnin</name>
<affiliation wicri:level="3"><nlm:aff id="aff3">Laboratori Clínic Department, Hospital Universitari de Bellvitge, Barcelona, Spain;</nlm:aff>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Laboratori Clínic Department, Hospital Universitari de Bellvitge, Barcelona</wicri:regionArea>
<placeName><settlement type="city">Barcelone</settlement>
<region nuts="2" type="region">Catalogne</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wallemacq, Pierre" sort="Wallemacq, Pierre" uniqKey="Wallemacq P" first="Pierre" last="Wallemacq">Pierre Wallemacq</name>
<affiliation wicri:level="3"><nlm:aff id="aff4">Cliniques Universitaires St. Luc, Louvain Toxicology and Applied Pharmacology, Université Catholique de Louvain, Brussels, Belgium;</nlm:aff>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Cliniques Universitaires St. Luc, Louvain Toxicology and Applied Pharmacology, Université Catholique de Louvain, Brussels</wicri:regionArea>
<placeName><settlement type="city">Bruxelles</settlement>
<region nuts="2">Région de Bruxelles-Capitale</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Orth, Matthias" sort="Orth, Matthias" uniqKey="Orth M" first="Matthias" last="Orth">Matthias Orth</name>
<affiliation wicri:level="3"><nlm:aff id="aff5">Institue for Laboratory Medicine, Marienhospital, Stuttgart;</nlm:aff>
<country>Allemagne</country>
<placeName><settlement type="city">Stuttgart</settlement>
<region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Stuttgart</region>
</placeName>
<wicri:orgArea>Institue for Laboratory Medicine, Marienhospital</wicri:orgArea>
</affiliation>
</author>
<author><name sortKey="Widmann, Monika" sort="Widmann, Monika" uniqKey="Widmann M" first="Monika" last="Widmann">Monika Widmann</name>
<affiliation wicri:level="3"><nlm:aff wicri:cut="; and" id="aff6">Roche Diagnostics GmbH, Mannheim, Germany</nlm:aff>
<country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Roche Diagnostics GmbH, Mannheim</wicri:regionArea>
<placeName><region type="land" nuts="1">Bade-Wurtemberg</region>
<region type="district" nuts="2">District de Karlsruhe</region>
<settlement type="city">Mannheim</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Verstraete, Alain G" sort="Verstraete, Alain G" uniqKey="Verstraete A" first="Alain G." last="Verstraete">Alain G. Verstraete</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">Department of Laboratory Medicine, Ghent University and Ghent University Hospital, Belgium.</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Belgique</country>
<wicri:regionArea>Department of Laboratory Medicine, Ghent University and Ghent University Hospital</wicri:regionArea>
<wicri:noRegion>Ghent University and Ghent University Hospital</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Therapeutic Drug Monitoring</title>
<idno type="ISSN">0163-4356</idno>
<idno type="eISSN">1536-3694</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Supplemental Digital Content is Available in the Text.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Abendroth, D" uniqKey="Abendroth D">D Abendroth</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Citterio, F" uniqKey="Citterio F">F Citterio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Morris, Rg" uniqKey="Morris R">RG Morris</name>
</author>
<author><name sortKey="Holt, Dw" uniqKey="Holt D">DW Holt</name>
</author>
<author><name sortKey="Armstrong, Vw" uniqKey="Armstrong V">VW Armstrong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Andrews, Dj" uniqKey="Andrews D">DJ Andrews</name>
</author>
<author><name sortKey="Cramb, R" uniqKey="Cramb R">R Cramb</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ekberg, H" uniqKey="Ekberg H">H Ekberg</name>
</author>
<author><name sortKey="Tedesco Silva, H" uniqKey="Tedesco Silva H">H Tedesco-Silva</name>
</author>
<author><name sortKey="Demirbas, A" uniqKey="Demirbas A">A Demirbas</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marin G Mez, Lm" uniqKey="Marin G Mez L">LM Marín Gómez</name>
</author>
<author><name sortKey="Loinaz Segurota, C" uniqKey="Loinaz Segurota C">C Loinaz Segurota</name>
</author>
<author><name sortKey="Moreno Gonzalez, E" uniqKey="Moreno Gonzalez E">E Moreno González</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mesar, I" uniqKey="Mesar I">I Mesar</name>
</author>
<author><name sortKey="Basi Juki, N" uniqKey="Basi Juki N">N Bašić-Jukić</name>
</author>
<author><name sortKey="Bubi Filipi, L" uniqKey="Bubi Filipi L">L Bubić-Filipi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chadban, S" uniqKey="Chadban S">S Chadban</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ghisdal, L" uniqKey="Ghisdal L">L Ghisdal</name>
</author>
<author><name sortKey="Bouchta, Nb" uniqKey="Bouchta N">NB Bouchta</name>
</author>
<author><name sortKey="Broeders, N" uniqKey="Broeders N">N Broeders</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moore, R" uniqKey="Moore R">R Moore</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vincenti, F" uniqKey="Vincenti F">F Vincenti</name>
</author>
<author><name sortKey="Friman, S" uniqKey="Friman S">S Friman</name>
</author>
<author><name sortKey="Scheuermann, E" uniqKey="Scheuermann E">E Scheuermann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Holt, Dw" uniqKey="Holt D">DW Holt</name>
</author>
<author><name sortKey="Marwaha, G" uniqKey="Marwaha G">G Marwaha</name>
</author>
<author><name sortKey="Jones, K" uniqKey="Jones K">K Jones</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Leland, Jk" uniqKey="Leland J">JK Leland</name>
</author>
<author><name sortKey="Powell, Mj" uniqKey="Powell M">MJ Powell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bieglmayer, C" uniqKey="Bieglmayer C">C Bieglmayer</name>
</author>
<author><name sortKey="Chan, Dw" uniqKey="Chan D">DW Chan</name>
</author>
<author><name sortKey="Sokoll, L" uniqKey="Sokoll L">L Sokoll</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Annesley, Tm" uniqKey="Annesley T">TM Annesley</name>
</author>
<author><name sortKey="Clayton, L" uniqKey="Clayton L">L Clayton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Streit, F" uniqKey="Streit F">F Streit</name>
</author>
<author><name sortKey="Armstrong, Vw" uniqKey="Armstrong V">VW Armstrong</name>
</author>
<author><name sortKey="Oellerich, M" uniqKey="Oellerich M">M Oellerich</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Kunst, A" uniqKey="Kunst A">A Kunst</name>
</author>
<author><name sortKey="Busse Grawitz, A" uniqKey="Busse Grawitz A">A Busse Grawitz</name>
</author>
<author><name sortKey="Engeldinger, W" uniqKey="Engeldinger W">W Engeldinger</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oellerich, M" uniqKey="Oellerich M">M Oellerich</name>
</author>
<author><name sortKey="Armstrong, Vw" uniqKey="Armstrong V">VW Armstrong</name>
</author>
<author><name sortKey="Kahan, B" uniqKey="Kahan B">B Kahan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ekins, Rp" uniqKey="Ekins R">RP Ekins</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Wallemacq, P" uniqKey="Wallemacq P">P Wallemacq</name>
</author>
<author><name sortKey="Maine, Gt" uniqKey="Maine G">GT Maine</name>
</author>
<author><name sortKey="Berg, K" uniqKey="Berg K">K Berg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Glick, Mr" uniqKey="Glick M">MR Glick</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Peter, A" uniqKey="Peter A">A Peter</name>
</author>
<author><name sortKey="Shipkova, M" uniqKey="Shipkova M">M Shipkova</name>
</author>
<author><name sortKey="Wieland, E" uniqKey="Wieland E">E Wieland</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bartoli, A" uniqKey="Bartoli A">A Bartoli</name>
</author>
<author><name sortKey="Molinaro, M" uniqKey="Molinaro M">M Molinaro</name>
</author>
<author><name sortKey="Visai, L" uniqKey="Visai L">L Visai</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Knight, Sr" uniqKey="Knight S">SR Knight</name>
</author>
<author><name sortKey="Morris, Pj" uniqKey="Morris P">PJ Morris</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oellerich, M" uniqKey="Oellerich M">M Oellerich</name>
</author>
<author><name sortKey="Armstrong, Vw" uniqKey="Armstrong V">VW Armstrong</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Ther Drug Monit</journal-id>
<journal-id journal-id-type="iso-abbrev">Ther Drug Monit</journal-id>
<journal-id journal-id-type="publisher-id">tdm</journal-id>
<journal-title-group><journal-title>Therapeutic Drug Monitoring</journal-title>
</journal-title-group>
<issn pub-type="ppub">0163-4356</issn>
<issn pub-type="epub">1536-3694</issn>
<publisher><publisher-name>Therapeutic Drug Monitoring</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24646730</article-id>
<article-id pub-id-type="pmc">4218761</article-id>
<article-id pub-id-type="publisher-id">TDM13-121-Oe</article-id>
<article-id pub-id-type="doi">10.1097/FTD.0000000000000068</article-id>
<article-id pub-id-type="art-access-id">00013</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Vogeser</surname>
<given-names>Michael</given-names>
</name>
<degrees>Dr. med.</degrees>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Shipkova</surname>
<given-names>Maria</given-names>
</name>
<degrees>Dr. med.</degrees>
<xref ref-type="aff" rid="aff2">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Rigo-Bonnin</surname>
<given-names>Raül</given-names>
</name>
<degrees>PhD</degrees>
<xref ref-type="aff" rid="aff3">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wallemacq</surname>
<given-names>Pierre</given-names>
</name>
<degrees>PhD</degrees>
<xref ref-type="aff" rid="aff4">§</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Orth</surname>
<given-names>Matthias</given-names>
</name>
<degrees>Dr. med.</degrees>
<xref ref-type="aff" rid="aff5">¶</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Widmann</surname>
<given-names>Monika</given-names>
</name>
<degrees>ChTech</degrees>
<xref ref-type="aff" rid="aff6">‖</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Verstraete</surname>
<given-names>Alain G.</given-names>
</name>
<degrees>Dr. med., PhD</degrees>
<xref ref-type="aff" rid="aff7">**</xref>
</contrib>
<aff id="aff1"><label>*</label>
Hospital of the University of Munich, Institute of Laboratory Medicine;</aff>
<aff id="aff2"><label>†</label>
Central Institute of Clinical Chemistry and Laboratory Medicine, Klinikum-Stuttgart, Germany;</aff>
<aff id="aff3"><label>‡</label>
Laboratori Clínic Department, Hospital Universitari de Bellvitge, Barcelona, Spain;</aff>
<aff id="aff4"><label>§</label>
Cliniques Universitaires St. Luc, Louvain Toxicology and Applied Pharmacology, Université Catholique de Louvain, Brussels, Belgium;</aff>
<aff id="aff5"><label>¶</label>
Institue for Laboratory Medicine, Marienhospital, Stuttgart;</aff>
<aff id="aff6"><label>‖</label>
Roche Diagnostics GmbH, Mannheim, Germany; and</aff>
<aff id="aff7"><label>**</label>
Department of Laboratory Medicine, Ghent University and Ghent University Hospital, Belgium.</aff>
</contrib-group>
<author-notes><corresp>Correspondence: Alain G. Verstraete, Dr. med., PhD, Department of Laboratory Medicine, Ghent University, Ghent University Hospital, De Pintelaan 185, B-9000 Ghent, Belgium (e-mail: <email>alain.verstraete@ugent.be</email>
).</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>10</month>
<year>2014</year>
</pub-date>
<pub-date pub-type="epub"><day>25</day>
<month>9</month>
<year>2014</year>
</pub-date>
<volume>36</volume>
<issue>5</issue>
<fpage>640</fpage>
<lpage>650</lpage>
<history><date date-type="received"><day>28</day>
<month>10</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>12</day>
<month>3</month>
<year>2014</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2014 by Lippincott Williams & Wilkins</copyright-statement>
<copyright-year>2014</copyright-year>
<license license-type="open-access"><license-p>This is an open access article distributed under the terms of the Creative Commons Attribution-Noncommercial No Derivative 3.0 License, which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially.</license-p>
</license>
</permissions>
<self-uri xlink:type="simple" xlink:href="tdm-36-640.pdf"></self-uri>
<abstract abstract-type="toc"><p>Supplemental Digital Content is Available in the Text.</p>
</abstract>
<abstract><sec><title>Background:</title>
<p>Cyclosporine A (CsA) is used as a posttransplantation immunosuppressant drug, and careful monitoring of CsA concentration in whole blood is essential. A new automated electrochemiluminescence immunoassay (ECLIA) for CsA measurement has been assessed in a multicenter evaluation.</p>
</sec>
<sec><title>Methods:</title>
<p>Residual EDTA whole blood samples from patients undergoing CsA therapy after organ transplant were used in assay evaluation at 5 clinical laboratories in Europe. Experiments included imprecision according to CLSI EP5-A2 (within-run and intermediate), lower limit of quantification, linearity according to CLSI EP6-A, and recovery of commercial external quality control samples. In addition, comparisons to liquid chromatography-tandem mass spectrometry methods in routine use at each investigational site and to commercial chemiluminescent microparticle immunoassay and antibody-conjugated magnetic immunoassay methods were performed.</p>
</sec>
<sec><title>Results:</title>
<p>Imprecision testing gave coefficients of variation of less than 9% in the 30–2000 mcg/L range for both within-run and intermediate imprecision. Lower limit of quantification of 6.8 mcg/L at one investigational site and 1.8 mcg/L at a second site at 20% coefficient of variation were observed. Linearity was measured over the concentration range 0–2000 mcg/L, yielding a deviation of less than ±12%. External quality control sample recovery by ECLIA was 93%–114% of LC-MS/MS sample recovery. Deming regression analysis of ECLIA method comparison to combined LC-MS/MS results yielded a slope of 1.04 [95% confidence interval (CI), 1.03–1.06] and intercept of 2.8 mcg/L (95% CI, 1.5–4.1 mcg/L). Comparison to chemiluminescent microparticle immunoassay yielded a slope of 0.87 (95% CI, 0.85–0.89) and intercept of 1.4 mcg/L (95% CI, −0.89 to 3.7 mcg/L); comparison to antibody-conjugated magnetic immunoassay yielded a slope of 0.96 (95% CI, 0.93–0.98) and intercept of −4.2 mcg/L (95% CI, −7.1 to −1.2 mcg/L).</p>
</sec>
<sec><title>Conclusions:</title>
<p>The data from this multicenter evaluation indicate that the new ECLIA-based cyclosporine assay is fit for its purpose, the therapeutic monitoring of CsA.</p>
</sec>
</abstract>
<kwd-group><title>Key Words:</title>
<kwd>cyclosporine</kwd>
<kwd>immunoassay</kwd>
<kwd>therapeutic drug monitoring</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>OPEN-ACCESS</meta-name>
<meta-value>TRUE</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Allemagne</li>
<li>Belgique</li>
<li>Espagne</li>
</country>
<region><li>Bade-Wurtemberg</li>
<li>Bavière</li>
<li>Catalogne</li>
<li>District de Haute-Bavière</li>
<li>District de Karlsruhe</li>
<li>District de Stuttgart</li>
<li>Région de Bruxelles-Capitale</li>
</region>
<settlement><li>Barcelone</li>
<li>Bruxelles</li>
<li>Mannheim</li>
<li>Munich</li>
<li>Stuttgart</li>
</settlement>
<orgName><li>Université Louis-et-Maximilien de Munich</li>
</orgName>
</list>
<tree><country name="Allemagne"><region name="Bavière"><name sortKey="Vogeser, Michael" sort="Vogeser, Michael" uniqKey="Vogeser M" first="Michael" last="Vogeser">Michael Vogeser</name>
</region>
<name sortKey="Orth, Matthias" sort="Orth, Matthias" uniqKey="Orth M" first="Matthias" last="Orth">Matthias Orth</name>
<name sortKey="Shipkova, Maria" sort="Shipkova, Maria" uniqKey="Shipkova M" first="Maria" last="Shipkova">Maria Shipkova</name>
<name sortKey="Widmann, Monika" sort="Widmann, Monika" uniqKey="Widmann M" first="Monika" last="Widmann">Monika Widmann</name>
</country>
<country name="Espagne"><region name="Catalogne"><name sortKey="Rigo Bonnin, Raul" sort="Rigo Bonnin, Raul" uniqKey="Rigo Bonnin R" first="Raül" last="Rigo-Bonnin">Raül Rigo-Bonnin</name>
</region>
</country>
<country name="Belgique"><region name="Région de Bruxelles-Capitale"><name sortKey="Wallemacq, Pierre" sort="Wallemacq, Pierre" uniqKey="Wallemacq P" first="Pierre" last="Wallemacq">Pierre Wallemacq</name>
</region>
<name sortKey="Verstraete, Alain G" sort="Verstraete, Alain G" uniqKey="Verstraete A" first="Alain G." last="Verstraete">Alain G. Verstraete</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000195 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000195 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Belgique |area= OpenAccessBelV2 |flux= Pmc |étape= Checkpoint |type= RBID |clé= PMC:4218761 |texte= Multicenter Analytical Evaluation of the Automated Electrochemiluminescence Immunoassay for Cyclosporine }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:24646730" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a OpenAccessBelV2
![]() | This area was generated with Dilib version V0.6.25. | ![]() |