Peroxidases identified in a subtractive cDNA library approach show tissue-specific transcript abundance and enzyme activity during seed germination of Lepidium sativum
Identifieur interne : 000D81 ( Main/Exploration ); précédent : 000D80; suivant : 000D82Peroxidases identified in a subtractive cDNA library approach show tissue-specific transcript abundance and enzyme activity during seed germination of Lepidium sativum
Auteurs : Ada Linkies [Allemagne] ; Uta Schuster-Sherpa [Allemagne] ; Stefanie Tintelnot [Allemagne] ; Gerhard Leubner-Metzger [Allemagne] ; Kerstin Mller [Allemagne]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2009-10-30.
Abstract
The micropylar endosperm is a major regulator of seed germination in endospermic species, to which the close Brassicaceae relatives Arabidopsis thaliana and Lepidium sativum (cress) belong. Cress seeds are about 20 times larger than the seeds of Arabidopsis. This advantage was used to construct a tissue-specific subtractive cDNA library of transcripts that are up-regulated late in the germination process specifically in the micropylar endosperm of cress seeds. The library showed that a number of transcripts known to be up-regulated late during germination are up-regulated in the micropylar endosperm cap. Detailed germination kinetics of SALK lines carrying insertions in genes present in our library showed that the identified transcripts do indeed play roles during germination. Three peroxidases were present in the library. These peroxidases were identified as orthologues of Arabidopsis AtAPX01, AtPrx16, and AtPrxIIE. The corresponding SALK lines displayed significant germination phenotypes. Their transcripts were quantified in specific cress seed tissues during germination in the presence and absence of ABA and they were found to be regulated in a tissue-specific manner. Peroxidase activity, and particularly its regulation by ABA, also differed between radicles and micropylar endosperm caps. Possible implications of this tissue-specificity are discussed.
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DOI: 10.1093/jxb/erp318
Affiliations:
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when="2009-10-30">2009-10-30</date><biblScope unit="volume">61</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="491">491</biblScope><biblScope unit="page" to="502">502</biblScope></imprint><idno type="ISSN">0022-0957</idno></series><idno type="istex">A6FA16DA61B9222984C3ED81569665B1B91B291B</idno><idno type="DOI">10.1093/jxb/erp318</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The micropylar endosperm is a major regulator of seed germination in endospermic species, to which the close Brassicaceae relatives Arabidopsis thaliana and Lepidium sativum (cress) belong. Cress seeds are about 20 times larger than the seeds of Arabidopsis. This advantage was used to construct a tissue-specific subtractive cDNA library of transcripts that are up-regulated late in the germination process specifically in the micropylar endosperm of cress seeds. The library showed that a number of transcripts known to be up-regulated late during germination are up-regulated in the micropylar endosperm cap. Detailed germination kinetics of SALK lines carrying insertions in genes present in our library showed that the identified transcripts do indeed play roles during germination. Three peroxidases were present in the library. These peroxidases were identified as orthologues of Arabidopsis AtAPX01, AtPrx16, and AtPrxIIE. The corresponding SALK lines displayed significant germination phenotypes. Their transcripts were quantified in specific cress seed tissues during germination in the presence and absence of ABA and they were found to be regulated in a tissue-specific manner. Peroxidase activity, and particularly its regulation by ABA, also differed between radicles and micropylar endosperm caps. Possible implications of this tissue-specificity are discussed.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Bade-Wurtemberg</li><li>District de Fribourg-en-Brisgau</li></region><settlement><li>Fribourg-en-Brisgau</li></settlement></list><tree><country name="Allemagne"><region name="Bade-Wurtemberg"><name sortKey="Linkies, Ada" sort="Linkies, Ada" uniqKey="Linkies A" first="Ada" last="Linkies">Ada Linkies</name></region><name sortKey="Leubner Metzger, Gerhard" sort="Leubner Metzger, Gerhard" uniqKey="Leubner Metzger G" first="Gerhard" last="Leubner-Metzger">Gerhard Leubner-Metzger</name><name sortKey="Mller, Kerstin" sort="Mller, Kerstin" uniqKey="Mller K" first="Kerstin" last="Mller">Kerstin Mller</name><name sortKey="Schuster Sherpa, Uta" sort="Schuster Sherpa, Uta" uniqKey="Schuster Sherpa U" first="Uta" last="Schuster-Sherpa">Uta Schuster-Sherpa</name><name sortKey="Tintelnot, Stefanie" sort="Tintelnot, Stefanie" uniqKey="Tintelnot S" first="Stefanie" last="Tintelnot">Stefanie Tintelnot</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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