Detection of single DNA molecules by multicolor quantum-dot end-labeling
Identifieur interne : 000990 ( Istex/Corpus ); précédent : 000989; suivant : 000991Detection of single DNA molecules by multicolor quantum-dot end-labeling
Auteurs : Aure Lien Crut ; Be Ne Dicte Ge Ron-Landre ; Isabelle Bonnet ; Ste Phane Bonneau ; Pierre Desbiolles ; Christophe EscudeSource :
- Nucleic Acids Research [ 0305-1048 ] ; 2005.
Abstract
Observation of DNA–protein interactions by single molecule fluorescence microscopy is usually performed by using fluorescent DNA binding agents. However, such dyes have been shown to induce cleavage of the DNA molecule and perturb its interactions with proteins. A new method for the detection of surface-attached DNA molecules by fluorescence microscopy is introduced in this paper. Biotin- and/or digoxigenin-modified DNA fragments are covalently linked at both extremities of a DNA molecule via sequence-specific hybridization and ligation. After the modified DNA molecules have been stretched on a glass surface, their ends are visualized by multicolor fluorescence microscopy using conjugated quantum dots (QD). We demonstrate that under carefully selected conditions, the position and orientation of individual DNA molecules can be inferred with good efficiency from the QD fluorescence signals alone. This is achieved by selecting QD pairs that have the distance and direction expected for the combed DNA molecules. Direct observation of single DNA molecules in the absence of DNA staining agent opens new possibilities in the fundamental study of DNA–protein interactions. This work also documents new possibilities regarding the use of QD for nucleic acid detection and analysis.
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DOI: 10.1093/nar/gni097
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ISTEX:6CDC46285C7526CA1CB86DD6590969430BEB01F4Le document en format XML
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Tel: +33 1 40793774; Fax: +33 1 40793705; Email: escude@mnhn.fr</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Nucleic Acids Research</title><title level="j" type="abbrev">Nucl. Acids Res.</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2005">2005</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="98">e98</biblScope><biblScope unit="page" to="98">e98</biblScope></imprint><idno type="ISSN">0305-1048</idno></series><idno type="istex">6CDC46285C7526CA1CB86DD6590969430BEB01F4</idno><idno type="DOI">10.1093/nar/gni097</idno><idno type="local">gni097</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Observation of DNA–protein interactions by single molecule fluorescence microscopy is usually performed by using fluorescent DNA binding agents. However, such dyes have been shown to induce cleavage of the DNA molecule and perturb its interactions with proteins. A new method for the detection of surface-attached DNA molecules by fluorescence microscopy is introduced in this paper. Biotin- and/or digoxigenin-modified DNA fragments are covalently linked at both extremities of a DNA molecule via sequence-specific hybridization and ligation. After the modified DNA molecules have been stretched on a glass surface, their ends are visualized by multicolor fluorescence microscopy using conjugated quantum dots (QD). We demonstrate that under carefully selected conditions, the position and orientation of individual DNA molecules can be inferred with good efficiency from the QD fluorescence signals alone. This is achieved by selecting QD pairs that have the distance and direction expected for the combed DNA molecules. Direct observation of single DNA molecules in the absence of DNA staining agent opens new possibilities in the fundamental study of DNA–protein interactions. This work also documents new possibilities regarding the use of QD for nucleic acid detection and analysis.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Aurélien Crut</name><affiliations><json:string>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</json:string></affiliations></json:item><json:item><name>Bénédicte Géron-Landre</name><affiliations><json:string>Laboratoire ‘Régulation et Dynamique des Génomes’, Département ‘Régulations, Développement et Diversité Moléculaire’, USM 0503 Muséum National d'Histoire Naturelle, CNRS UMR5153, INSERM U565 Case Postale 26, 43 rue Cuvier, F-75231 Paris Cedex 05, France</json:string></affiliations></json:item><json:item><name>Isabelle Bonnet</name><affiliations><json:string>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</json:string></affiliations></json:item><json:item><name>Stéphane Bonneau</name><affiliations><json:string>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</json:string><json:string>CEREMADE, Université Paris IX -Dauphine F-75775 Paris, France</json:string></affiliations></json:item><json:item><name>Pierre Desbiolles</name><affiliations><json:string>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</json:string></affiliations></json:item><json:item><name>Christophe Escudé</name><affiliations><json:string>Laboratoire ‘Régulation et Dynamique des Génomes’, Département ‘Régulations, Développement et Diversité Moléculaire’, USM 0503 Muséum National d'Histoire Naturelle, CNRS UMR5153, INSERM U565 Case Postale 26, 43 rue Cuvier, F-75231 Paris Cedex 05, France</json:string><json:string>To whom correspondence should be addressed. Tel: +33 1 40793774; Fax: +33 1 40793705; Email: escude@mnhn.fr</json:string><json:string>E-mail: escude@mnhn.fr</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>4</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>other</json:string></originalGenre><abstract>Observation of DNA–protein interactions by single molecule fluorescence microscopy is usually performed by using fluorescent DNA binding agents. However, such dyes have been shown to induce cleavage of the DNA molecule and perturb its interactions with proteins. A new method for the detection of surface-attached DNA molecules by fluorescence microscopy is introduced in this paper. Biotin- and/or digoxigenin-modified DNA fragments are covalently linked at both extremities of a DNA molecule via sequence-specific hybridization and ligation. After the modified DNA molecules have been stretched on a glass surface, their ends are visualized by multicolor fluorescence microscopy using conjugated quantum dots (QD). We demonstrate that under carefully selected conditions, the position and orientation of individual DNA molecules can be inferred with good efficiency from the QD fluorescence signals alone. This is achieved by selecting QD pairs that have the distance and direction expected for the combed DNA molecules. Direct observation of single DNA molecules in the absence of DNA staining agent opens new possibilities in the fundamental study of DNA–protein interactions. 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Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org</p></availability><date>2005</date></publicationStmt><notesStmt><note>*To whom correspondence should be addressed. Tel: +33 1 40793774; Fax: +33 1 40793705; Email: escude@mnhn.fr</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Detection of single DNA molecules by multicolor quantum-dot end-labeling</title><author xml:id="author-1"><persName><forename type="first">Aurélien</forename><surname>Crut</surname></persName><affiliation>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">Bénédicte</forename><surname>Géron-Landre</surname></persName><affiliation>Laboratoire ‘Régulation et Dynamique des Génomes’, Département ‘Régulations, Développement et Diversité Moléculaire’, USM 0503 Muséum National d'Histoire Naturelle, CNRS UMR5153, INSERM U565 Case Postale 26, 43 rue Cuvier, F-75231 Paris Cedex 05, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">Isabelle</forename><surname>Bonnet</surname></persName><affiliation>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">Stéphane</forename><surname>Bonneau</surname></persName><affiliation>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</affiliation><affiliation>CEREMADE, Université Paris IX -Dauphine F-75775 Paris, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">Pierre</forename><surname>Desbiolles</surname></persName><affiliation>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">Christophe</forename><surname>Escudé</surname></persName><affiliation>Laboratoire ‘Régulation et Dynamique des Génomes’, Département ‘Régulations, Développement et Diversité Moléculaire’, USM 0503 Muséum National d'Histoire Naturelle, CNRS UMR5153, INSERM U565 Case Postale 26, 43 rue Cuvier, F-75231 Paris Cedex 05, France</affiliation><affiliation>To whom correspondence should be addressed. Tel: +33 1 40793774; Fax: +33 1 40793705; Email: escude@mnhn.fr</affiliation></author></analytic><monogr><title level="j">Nucleic Acids Research</title><title level="j" type="abbrev">Nucl. Acids Res.</title><idno type="pISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2005"></date><biblScope unit="volume">33</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="98">e98</biblScope><biblScope unit="page" to="98">e98</biblScope></imprint></monogr><idno type="istex">6CDC46285C7526CA1CB86DD6590969430BEB01F4</idno><idno type="DOI">10.1093/nar/gni097</idno><idno type="local">gni097</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2005</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Observation of DNA–protein interactions by single molecule fluorescence microscopy is usually performed by using fluorescent DNA binding agents. However, such dyes have been shown to induce cleavage of the DNA molecule and perturb its interactions with proteins. A new method for the detection of surface-attached DNA molecules by fluorescence microscopy is introduced in this paper. Biotin- and/or digoxigenin-modified DNA fragments are covalently linked at both extremities of a DNA molecule via sequence-specific hybridization and ligation. After the modified DNA molecules have been stretched on a glass surface, their ends are visualized by multicolor fluorescence microscopy using conjugated quantum dots (QD). We demonstrate that under carefully selected conditions, the position and orientation of individual DNA molecules can be inferred with good efficiency from the QD fluorescence signals alone. This is achieved by selecting QD pairs that have the distance and direction expected for the combed DNA molecules. Direct observation of single DNA molecules in the absence of DNA staining agent opens new possibilities in the fundamental study of DNA–protein interactions. This work also documents new possibilities regarding the use of QD for nucleic acid detection and analysis.</p></abstract><textClass><keywords scheme="keyword"><list><head>hwp-journal-coll</head><item><term>4</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2005">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-10-14">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/6CDC46285C7526CA1CB86DD6590969430BEB01F4/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="US-ASCII"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article xml:lang="en" article-type="other"><front><journal-meta><journal-id journal-id-type="hwp">nar</journal-id><journal-id journal-id-type="nlm-ta">Nucleic Acids Res</journal-id><journal-id journal-id-type="publisher-id">nar</journal-id><journal-title>Nucleic Acids Research</journal-title><abbrev-journal-title abbrev-type="publisher">Nucl. Acids Res.</abbrev-journal-title><issn pub-type="ppub">0305-1048</issn><issn pub-type="epub">1362-4962</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="other">gni097</article-id><article-id pub-id-type="doi">10.1093/nar/gni097</article-id><article-categories><subj-group subj-group-type="heading"><subject>Methods Online</subject></subj-group><subj-group subj-group-type="hwp-journal-coll"><subject>4</subject></subj-group></article-categories><title-group><article-title>Detection of single DNA molecules by multicolor quantum-dot end-labeling</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Crut</surname><given-names>Aurélien</given-names></name><xref rid="AU1">1</xref></contrib><contrib contrib-type="author"><name><surname>Géron-Landre</surname><given-names>Bénédicte</given-names></name><xref rid="AU2">2</xref></contrib><contrib contrib-type="author"><name><surname>Bonnet</surname><given-names>Isabelle</given-names></name><xref rid="AU1">1</xref></contrib><contrib contrib-type="author"><name><surname>Bonneau</surname><given-names>Stéphane</given-names></name><xref rid="AU1">1</xref><xref rid="AU3">3</xref></contrib><contrib contrib-type="author"><name><surname>Desbiolles</surname><given-names>Pierre</given-names></name><xref rid="AU1">1</xref></contrib><contrib contrib-type="author"><name><surname>Escudé</surname><given-names>Christophe</given-names></name><xref rid="AU2">2</xref><xref rid="COR1">*</xref></contrib><aff id="AU1"><sup>1</sup>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</aff><aff id="AU2"><sup>2</sup>Laboratoire ‘Régulation et Dynamique des Génomes’, Département ‘Régulations, Développement et Diversité Moléculaire’, USM 0503 Muséum National d'Histoire Naturelle, CNRS UMR5153, INSERM U565 Case Postale 26, 43 rue Cuvier, F-75231 Paris Cedex 05, France</aff><aff id="AU3"><sup>3</sup>CEREMADE, Université Paris IX -Dauphine F-75775 Paris, France</aff></contrib-group><author-notes><corresp id="COR1"><sup>*</sup>To whom correspondence should be addressed. Tel: +33 1 40793774; Fax: +33 1 40793705; Email: <ext-link xlink:href="escude@mnhn.fr" ext-link-type="email">escude@mnhn.fr</ext-link></corresp></author-notes><pub-date pub-type="ppub"><year>2005</year></pub-date><volume>33</volume><issue>11</issue><fpage>e98</fpage><lpage>e98</lpage><history><date date-type="accepted"><day>31</day><month>5</month><year>2005</year></date><date date-type="received"><day>13</day><month>4</month><year>2005</year></date><date date-type="rev-recd"><day>31</day><month>5</month><year>2005</year></date></history><permissions><copyright-statement>© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact <ext-link xlink:href="journals.permissions@oupjournals.org" ext-link-type="email">journals.permissions@oupjournals.org</ext-link></copyright-statement><copyright-year>2005</copyright-year><license license-type="open-access"><p></p></license></permissions><abstract xml:lang="en"><p>Observation of DNA–protein interactions by single molecule fluorescence microscopy is usually performed by using fluorescent DNA binding agents. However, such dyes have been shown to induce cleavage of the DNA molecule and perturb its interactions with proteins. A new method for the detection of surface-attached DNA molecules by fluorescence microscopy is introduced in this paper. Biotin- and/or digoxigenin-modified DNA fragments are covalently linked at both extremities of a DNA molecule via sequence-specific hybridization and ligation. After the modified DNA molecules have been stretched on a glass surface, their ends are visualized by multicolor fluorescence microscopy using conjugated quantum dots (QD). We demonstrate that under carefully selected conditions, the position and orientation of individual DNA molecules can be inferred with good efficiency from the QD fluorescence signals alone. This is achieved by selecting QD pairs that have the distance and direction expected for the combed DNA molecules. Direct observation of single DNA molecules in the absence of DNA staining agent opens new possibilities in the fundamental study of DNA–protein interactions. This work also documents new possibilities regarding the use of QD for nucleic acid detection and analysis.</p></abstract><custom-meta-wrap><custom-meta><meta-name>hwp-legacy-fpage</meta-name><meta-value>e98</meta-value></custom-meta><custom-meta><meta-name>cover-date</meta-name><meta-value></meta-value></custom-meta><custom-meta><meta-name>hwp-legacy-dochead</meta-name><meta-value>research-article</meta-value></custom-meta></custom-meta-wrap></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Detection of single DNA molecules by multicolor quantum-dot end-labeling</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Detection of single DNA molecules by multicolor quantum-dot end-labeling</title></titleInfo><name type="personal"><namePart type="given">Aurélien</namePart><namePart type="family">Crut</namePart><affiliation>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Bénédicte</namePart><namePart type="family">Géron-Landre</namePart><affiliation>Laboratoire ‘Régulation et Dynamique des Génomes’, Département ‘Régulations, Développement et Diversité Moléculaire’, USM 0503 Muséum National d'Histoire Naturelle, CNRS UMR5153, INSERM U565 Case Postale 26, 43 rue Cuvier, F-75231 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Isabelle</namePart><namePart type="family">Bonnet</namePart><affiliation>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stéphane</namePart><namePart type="family">Bonneau</namePart><affiliation>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</affiliation><affiliation>CEREMADE, Université Paris IX -Dauphine F-75775 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Pierre</namePart><namePart type="family">Desbiolles</namePart><affiliation>Laboratoire Kastler Brossel, Unité de Recherche de l'Ecole Normale Supérieure et de l'Université Pierre et Marie Curie, associée au CNRS, Département de Physique 24 rue Lhomond, F-75005 Paris, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Christophe</namePart><namePart type="family">Escudé</namePart><affiliation>Laboratoire ‘Régulation et Dynamique des Génomes’, Département ‘Régulations, Développement et Diversité Moléculaire’, USM 0503 Muséum National d'Histoire Naturelle, CNRS UMR5153, INSERM U565 Case Postale 26, 43 rue Cuvier, F-75231 Paris Cedex 05, France</affiliation><affiliation>To whom correspondence should be addressed. Tel: +33 1 40793774; Fax: +33 1 40793705; Email: escude@mnhn.fr</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="other" displayLabel="other"></genre><subject><genre>hwp-journal-coll</genre><topic>4</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2005</dateIssued><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Observation of DNA–protein interactions by single molecule fluorescence microscopy is usually performed by using fluorescent DNA binding agents. However, such dyes have been shown to induce cleavage of the DNA molecule and perturb its interactions with proteins. A new method for the detection of surface-attached DNA molecules by fluorescence microscopy is introduced in this paper. Biotin- and/or digoxigenin-modified DNA fragments are covalently linked at both extremities of a DNA molecule via sequence-specific hybridization and ligation. After the modified DNA molecules have been stretched on a glass surface, their ends are visualized by multicolor fluorescence microscopy using conjugated quantum dots (QD). We demonstrate that under carefully selected conditions, the position and orientation of individual DNA molecules can be inferred with good efficiency from the QD fluorescence signals alone. This is achieved by selecting QD pairs that have the distance and direction expected for the combed DNA molecules. Direct observation of single DNA molecules in the absence of DNA staining agent opens new possibilities in the fundamental study of DNA–protein interactions. This work also documents new possibilities regarding the use of QD for nucleic acid detection and analysis.</abstract><note type="author-notes">*To whom correspondence should be addressed. Tel: +33 1 40793774; Fax: +33 1 40793705; Email: escude@mnhn.fr</note><relatedItem type="host"><titleInfo><title>Nucleic Acids Research</title></titleInfo><titleInfo type="abbreviated"><title>Nucl. Acids Res.</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0305-1048</identifier><identifier type="eISSN">1362-4962</identifier><identifier type="PublisherID">nar</identifier><identifier type="PublisherID-hwp">nar</identifier><identifier type="PublisherID-nlm-ta">Nucleic Acids Res</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>33</number></detail><detail type="issue"><caption>no.</caption><number>11</number></detail><extent unit="pages"><start>e98</start><end>e98</end></extent></part></relatedItem><identifier type="istex">6CDC46285C7526CA1CB86DD6590969430BEB01F4</identifier><identifier type="DOI">10.1093/nar/gni097</identifier><identifier type="local">gni097</identifier><accessCondition type="use and reproduction" contentType="copyright">© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/6CDC46285C7526CA1CB86DD6590969430BEB01F4/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/6CDC46285C7526CA1CB86DD6590969430BEB01F4/annexes/gif</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/6CDC46285C7526CA1CB86DD6590969430BEB01F4/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">BIOCHEMISTRY & MOLECULAR BIOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/6CDC46285C7526CA1CB86DD6590969430BEB01F4/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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