Toxicity of used orthodontic archwires assessed by three-dimensional cell culture
Identifieur interne : 000568 ( Istex/Corpus ); précédent : 000567; suivant : 000569Toxicity of used orthodontic archwires assessed by three-dimensional cell culture
Auteurs : Bart Vande Vannet ; Nahid Mohebbian ; Heinrich WehrbeinSource :
- The European Journal of Orthodontics [ 0141-5387 ] ; 2006-08-11.
Abstract
The aim of the present study was to determine whether used orthodontic wires made of different materials cause toxicity and loss of viability on three-dimensional (3D) cell cultures. Three types of orthodontic wires, stainless steel, Nitinol, and TMA (n 9) which had been used clinically in fixed appliances for a period of 1 month, were retrieved at random from five patients. Both upper and lower archwires were collected and subjected to two different protocols: to assess toxicity, two pieces of each wire were placed on 3D cell cultures (reconstituted human epithelium); to investigate the possibility of cell damage, the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay was used and haematoxylin and eosin staining was performed to evaluate morphological changes. Copper wire served as the control to determine the morphology of severe toxicity, and native cell cultures and silk were used as the negative controls. Morphological evaluation of the native cell cultures revealed no toxic reactions. The ranking, from mild to severe toxicity was as follows: stainless steel < Nitinol TMA. There were no significant differences between TMA and Nitinol. The MTT assay revealed the following mean percentage values for viability: native cell line (negative control), 100; stainless steel, 102.25; TMA, 87.4; Nitinol, 85.3; and copper wire (positive control) 57.2. Histological evaluation of the 3D cell cultures showed no severe toxicity or loss of viability for any of the wires. However, relative comparison between the different wires revealed that stainless steel induced less toxicity/loss of viability compared with TMA and Nitinol wire.
Url:
DOI: 10.1093/ejo/cjl002
Links to Exploration step
ISTEX:F2A399013D09747F16E9673D25D5B4EE91973DADLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Toxicity of used orthodontic archwires assessed by three-dimensional cell culture</title><author><name sortKey="Vande Vannet, Bart" sort="Vande Vannet, Bart" uniqKey="Vande Vannet B" first="Bart" last="Vande Vannet">Bart Vande Vannet</name><affiliation><mods:affiliation>Department of Orthodontics, Free University of Brussels, Belgium</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: bart.vandevannet@pandora.be</mods:affiliation></affiliation></author><author><name sortKey="Mohebbian, Nahid" sort="Mohebbian, Nahid" uniqKey="Mohebbian N" first="Nahid" last="Mohebbian">Nahid Mohebbian</name><affiliation><mods:affiliation>Department of Restorative Dentistry, Free University of Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Wehrbein, Heinrich" sort="Wehrbein, Heinrich" uniqKey="Wehrbein H" first="Heinrich" last="Wehrbein">Heinrich Wehrbein</name><affiliation><mods:affiliation>Department of Orthodontics, University of Mainz, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F2A399013D09747F16E9673D25D5B4EE91973DAD</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1093/ejo/cjl002</idno><idno type="url">https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000568</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Toxicity of used orthodontic archwires assessed by three-dimensional cell culture</title><author><name sortKey="Vande Vannet, Bart" sort="Vande Vannet, Bart" uniqKey="Vande Vannet B" first="Bart" last="Vande Vannet">Bart Vande Vannet</name><affiliation><mods:affiliation>Department of Orthodontics, Free University of Brussels, Belgium</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: bart.vandevannet@pandora.be</mods:affiliation></affiliation></author><author><name sortKey="Mohebbian, Nahid" sort="Mohebbian, Nahid" uniqKey="Mohebbian N" first="Nahid" last="Mohebbian">Nahid Mohebbian</name><affiliation><mods:affiliation>Department of Restorative Dentistry, Free University of Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Wehrbein, Heinrich" sort="Wehrbein, Heinrich" uniqKey="Wehrbein H" first="Heinrich" last="Wehrbein">Heinrich Wehrbein</name><affiliation><mods:affiliation>Department of Orthodontics, University of Mainz, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">The European Journal of Orthodontics</title><idno type="ISSN">0141-5387</idno><idno type="eISSN">1460-2210</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2006-08-11">2006-08-11</date><biblScope unit="volume">28</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="426">426</biblScope><biblScope unit="page" to="432">432</biblScope></imprint><idno type="ISSN">0141-5387</idno></series><idno type="istex">F2A399013D09747F16E9673D25D5B4EE91973DAD</idno><idno type="DOI">10.1093/ejo/cjl002</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0141-5387</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The aim of the present study was to determine whether used orthodontic wires made of different materials cause toxicity and loss of viability on three-dimensional (3D) cell cultures. Three types of orthodontic wires, stainless steel, Nitinol, and TMA (n 9) which had been used clinically in fixed appliances for a period of 1 month, were retrieved at random from five patients. Both upper and lower archwires were collected and subjected to two different protocols: to assess toxicity, two pieces of each wire were placed on 3D cell cultures (reconstituted human epithelium); to investigate the possibility of cell damage, the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay was used and haematoxylin and eosin staining was performed to evaluate morphological changes. Copper wire served as the control to determine the morphology of severe toxicity, and native cell cultures and silk were used as the negative controls. Morphological evaluation of the native cell cultures revealed no toxic reactions. The ranking, from mild to severe toxicity was as follows: stainless steel < Nitinol TMA. There were no significant differences between TMA and Nitinol. The MTT assay revealed the following mean percentage values for viability: native cell line (negative control), 100; stainless steel, 102.25; TMA, 87.4; Nitinol, 85.3; and copper wire (positive control) 57.2. Histological evaluation of the 3D cell cultures showed no severe toxicity or loss of viability for any of the wires. However, relative comparison between the different wires revealed that stainless steel induced less toxicity/loss of viability compared with TMA and Nitinol wire.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Bart Vande Vannet</name><affiliations><json:string>Department of Orthodontics, Free University of Brussels, Belgium</json:string><json:string>E-mail: bart.vandevannet@pandora.be</json:string></affiliations></json:item><json:item><name>Nahid Mohebbian</name><affiliations><json:string>Department of Restorative Dentistry, Free University of Brussels, Belgium</json:string></affiliations></json:item><json:item><name>Heinrich Wehrbein</name><affiliations><json:string>Department of Orthodontics, University of Mainz, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Articles</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>The aim of the present study was to determine whether used orthodontic wires made of different materials cause toxicity and loss of viability on three-dimensional (3D) cell cultures. Three types of orthodontic wires, stainless steel, Nitinol, and TMA (n 9) which had been used clinically in fixed appliances for a period of 1 month, were retrieved at random from five patients. Both upper and lower archwires were collected and subjected to two different protocols: to assess toxicity, two pieces of each wire were placed on 3D cell cultures (reconstituted human epithelium); to investigate the possibility of cell damage, the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay was used and haematoxylin and eosin staining was performed to evaluate morphological changes. Copper wire served as the control to determine the morphology of severe toxicity, and native cell cultures and silk were used as the negative controls. Morphological evaluation of the native cell cultures revealed no toxic reactions. The ranking, from mild to severe toxicity was as follows: stainless steel > Nitinol TMA. There were no significant differences between TMA and Nitinol. The MTT assay revealed the following mean percentage values for viability: native cell line (negative control), 100; stainless steel, 102.25; TMA, 87.4; Nitinol, 85.3; and copper wire (positive control) 57.2. Histological evaluation of the 3D cell cultures showed no severe toxicity or loss of viability for any of the wires. However, relative comparison between the different wires revealed that stainless steel induced less toxicity/loss of viability compared with TMA and Nitinol wire.</abstract><qualityIndicators><score>6.97</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595 x 782 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>1</keywordCount><abstractCharCount>1668</abstractCharCount><pdfWordCount>3982</pdfWordCount><pdfCharCount>26819</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>249</abstractWordCount></qualityIndicators><title>Toxicity of used orthodontic archwires assessed by three-dimensional cell culture</title><genre><json:string>research-article</json:string></genre><host><volume>28</volume><publisherId><json:string>eortho</json:string></publisherId><pages><last>432</last><first>426</first></pages><issn><json:string>0141-5387</json:string></issn><issue>5</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1460-2210</json:string></eissn><title>The European Journal of Orthodontics</title></host><categories><wos><json:string>DENTISTRY, ORAL SURGERY & MEDICINE</json:string></wos></categories><publicationDate>2006</publicationDate><copyrightDate>2006</copyrightDate><doi><json:string>10.1093/ejo/cjl002</json:string></doi><id>F2A399013D09747F16E9673D25D5B4EE91973DAD</id><score>0.28621423</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Toxicity of used orthodontic archwires assessed by three-dimensional cell culture</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Oxford University Press</publisher><availability status="free"><p>Open Access</p></availability><date>2006-08-11</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Toxicity of used orthodontic archwires assessed by three-dimensional cell culture</title><author xml:id="author-1" corresp="yes"><persName><forename type="first">Bart</forename><surname>Vande Vannet</surname></persName><email>bart.vandevannet@pandora.be</email><affiliation>Department of Orthodontics, Free University of Brussels, Belgium</affiliation></author><author xml:id="author-2"><persName><forename type="first">Nahid</forename><surname>Mohebbian</surname></persName><affiliation>Department of Restorative Dentistry, Free University of Brussels, Belgium</affiliation></author><author xml:id="author-3"><persName><forename type="first">Heinrich</forename><surname>Wehrbein</surname></persName><affiliation>Department of Orthodontics, University of Mainz, Germany</affiliation></author></analytic><monogr><title level="j">The European Journal of Orthodontics</title><idno type="pISSN">0141-5387</idno><idno type="eISSN">1460-2210</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2006-08-11"></date><biblScope unit="volume">28</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="426">426</biblScope><biblScope unit="page" to="432">432</biblScope></imprint></monogr><idno type="istex">F2A399013D09747F16E9673D25D5B4EE91973DAD</idno><idno type="DOI">10.1093/ejo/cjl002</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2006-08-11</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>The aim of the present study was to determine whether used orthodontic wires made of different materials cause toxicity and loss of viability on three-dimensional (3D) cell cultures. Three types of orthodontic wires, stainless steel, Nitinol, and TMA (n 9) which had been used clinically in fixed appliances for a period of 1 month, were retrieved at random from five patients. Both upper and lower archwires were collected and subjected to two different protocols: to assess toxicity, two pieces of each wire were placed on 3D cell cultures (reconstituted human epithelium); to investigate the possibility of cell damage, the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay was used and haematoxylin and eosin staining was performed to evaluate morphological changes. Copper wire served as the control to determine the morphology of severe toxicity, and native cell cultures and silk were used as the negative controls. Morphological evaluation of the native cell cultures revealed no toxic reactions. The ranking, from mild to severe toxicity was as follows: stainless steel < Nitinol TMA. There were no significant differences between TMA and Nitinol. The MTT assay revealed the following mean percentage values for viability: native cell line (negative control), 100; stainless steel, 102.25; TMA, 87.4; Nitinol, 85.3; and copper wire (positive control) 57.2. Histological evaluation of the 3D cell cultures showed no severe toxicity or loss of viability for any of the wires. However, relative comparison between the different wires revealed that stainless steel induced less toxicity/loss of viability compared with TMA and Nitinol wire.</p></abstract><textClass><keywords scheme="keyword"><list><item><term>Articles</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2006-08-11">Created</change><change when="2006-08-11">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-10-14">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">eortho</journal-id><journal-id journal-id-type="publisher-id">eortho</journal-id><journal-title>The European Journal of Orthodontics</journal-title><issn pub-type="epub">1460-2210</issn><issn pub-type="ppub">0141-5387</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/ejo/cjl002</article-id><article-categories><subj-group><subject>Articles</subject></subj-group></article-categories><title-group><article-title>Toxicity of used orthodontic archwires assessed by three-dimensional cell culture</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Vande Vannet</surname><given-names>Bart</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Mohebbian</surname><given-names>Nahid</given-names></name><xref ref-type="aff" rid="aff2">**</xref></contrib><contrib contrib-type="author"><name><surname>Wehrbein</surname><given-names>Heinrich</given-names></name><xref ref-type="aff" rid="aff3">***</xref></contrib></contrib-group><aff id="aff1"><label>*</label>Department of Orthodontics, Free University of Brussels, Belgium</aff><aff id="aff2"><label>**</label>Department of Restorative Dentistry, Free University of Brussels, Belgium</aff><aff id="aff3"><label>***</label>Department of Orthodontics, University of Mainz, Germany</aff><author-notes><corresp><bold>Address for correspondence</bold> Bart Vande Vannet, Department of Orthodontics, Medical and Pharmaceutical Faculty, Free University of Brussels, Laarbeeklaan 103, B-1090 Brussels, Belgium. E-mail: <email>bart.vandevannet@pandora.be</email></corresp></author-notes><pub-date pub-type="epub-ppub"><month>10</month><year>2006</year></pub-date><pub-date pub-type="epub"><day>11</day><month>8</month><year>2006</year></pub-date><volume>28</volume><issue>5</issue><fpage>426</fpage><lpage>432</lpage><permissions><copyright-statement>© The Author 2006. Published by Oxford University Press on behalf of the European Orthodontic Society. All rights reserved.</copyright-statement><copyright-year>2006</copyright-year><license license-type="open-access"><p>The online version of this article has been published under an Open Access model. Users are entitled to use, reproduce, disseminate, or display the Open Access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact: journals.permissions@oxfordjournals.org</p></license></permissions><abstract><p>The aim of the present study was to determine whether used orthodontic wires made of different materials cause toxicity and loss of viability on three-dimensional (3D) cell cultures. Three types of orthodontic wires, stainless steel, Nitinol®, and TMA® (<italic>n</italic> = 9) which had been used clinically in fixed appliances for a period of 1 month, were retrieved at random from five patients. Both upper and lower archwires were collected and subjected to two different protocols: to assess toxicity, two pieces of each wire were placed on 3D cell cultures (reconstituted human epithelium); to investigate the possibility of cell damage, the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay was used and haematoxylin and eosin staining was performed to evaluate morphological changes. Copper wire served as the control to determine the morphology of severe toxicity, and native cell cultures and silk were used as the negative controls.</p><p>Morphological evaluation of the native cell cultures revealed no toxic reactions. The ranking, from mild to severe toxicity was as follows: stainless steel < Nitinol® = TMA®. There were no significant differences between TMA® and Nitinol®. The MTT assay revealed the following mean percentage values for viability: native cell line (negative control), 100; stainless steel, 102.25; TMA®, 87.4; Nitinol®, 85.3; and copper wire (positive control) 57.2. Histological evaluation of the 3D cell cultures showed no severe toxicity or loss of viability for any of the wires. However, relative comparison between the different wires revealed that stainless steel induced less toxicity/loss of viability compared with TMA® and Nitinol® wire.</p></abstract></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Toxicity of used orthodontic archwires assessed by three-dimensional cell culture</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Toxicity of used orthodontic archwires assessed by three-dimensional cell culture</title></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Bart</namePart><namePart type="family">Vande Vannet</namePart><affiliation>Department of Orthodontics, Free University of Brussels, Belgium</affiliation><affiliation>E-mail: bart.vandevannet@pandora.be</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nahid</namePart><namePart type="family">Mohebbian</namePart><affiliation>Department of Restorative Dentistry, Free University of Brussels, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Heinrich</namePart><namePart type="family">Wehrbein</namePart><affiliation>Department of Orthodontics, University of Mainz, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><subject><topic>Articles</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2006-08-11</dateIssued><dateCreated encoding="w3cdtf">2006-08-11</dateCreated><copyrightDate encoding="w3cdtf">2006</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>The aim of the present study was to determine whether used orthodontic wires made of different materials cause toxicity and loss of viability on three-dimensional (3D) cell cultures. Three types of orthodontic wires, stainless steel, Nitinol, and TMA (n 9) which had been used clinically in fixed appliances for a period of 1 month, were retrieved at random from five patients. Both upper and lower archwires were collected and subjected to two different protocols: to assess toxicity, two pieces of each wire were placed on 3D cell cultures (reconstituted human epithelium); to investigate the possibility of cell damage, the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay was used and haematoxylin and eosin staining was performed to evaluate morphological changes. Copper wire served as the control to determine the morphology of severe toxicity, and native cell cultures and silk were used as the negative controls. Morphological evaluation of the native cell cultures revealed no toxic reactions. The ranking, from mild to severe toxicity was as follows: stainless steel < Nitinol TMA. There were no significant differences between TMA and Nitinol. The MTT assay revealed the following mean percentage values for viability: native cell line (negative control), 100; stainless steel, 102.25; TMA, 87.4; Nitinol, 85.3; and copper wire (positive control) 57.2. Histological evaluation of the 3D cell cultures showed no severe toxicity or loss of viability for any of the wires. However, relative comparison between the different wires revealed that stainless steel induced less toxicity/loss of viability compared with TMA and Nitinol wire.</abstract><relatedItem type="host"><titleInfo><title>The European Journal of Orthodontics</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0141-5387</identifier><identifier type="eISSN">1460-2210</identifier><identifier type="PublisherID">eortho</identifier><identifier type="PublisherID-hwp">eortho</identifier><part><date>2006</date><detail type="volume"><caption>vol.</caption><number>28</number></detail><detail type="issue"><caption>no.</caption><number>5</number></detail><extent unit="pages"><start>426</start><end>432</end></extent></part></relatedItem><identifier type="istex">F2A399013D09747F16E9673D25D5B4EE91973DAD</identifier><identifier type="DOI">10.1093/ejo/cjl002</identifier><accessCondition type="use and reproduction" contentType="open-access">The online version of this article has been published under an Open Access model. Users are entitled to use, reproduce, disseminate, or display the Open Access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact: journals.permissions@oxfordjournals.org</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><covers><json:item><original>true</original><mimetype>image/tiff</mimetype><extension>tiff</extension><uri>https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/covers/tiff</uri></json:item></covers><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/annexes/gif</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">DENTISTRY, ORAL SURGERY & MEDICINE</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/F2A399013D09747F16E9673D25D5B4EE91973DAD/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000568 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000568 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:F2A399013D09747F16E9673D25D5B4EE91973DAD
|texte= Toxicity of used orthodontic archwires assessed by three-dimensional cell culture
}}
| This area was generated with Dilib version V0.6.25. |  |