Temporal Localization of Immunoreactive Transforming Growth Factor β1 in Normal Equine Skin and in Full‐Thickness Dermal Wounds
Identifieur interne : 000C28 ( Main/Exploration ); précédent : 000C27; suivant : 000C29Temporal Localization of Immunoreactive Transforming Growth Factor β1 in Normal Equine Skin and in Full‐Thickness Dermal Wounds
Auteurs : Christine L. Theoret [Canada] ; Spencer M. Barber [Canada] ; John R. Gordon [Canada]Source :
- Veterinary Surgery [ 0161-3499 ] ; 2002-05.
English descriptors
- Teeft :
- Aberrant tissue repair, Acute phase, American college, Appendage, Assoc equine practnr, Biopsy, Broblast growth factor, Broblasts, Brogenic growth factor, Cellular biology, Cellular components, Cutaneous epithelium, Diplomate acvs, Distal aspect, Epidermal, Epidermal appendages, Epidermal layers, Epithelial, Epithelial tips, Epithelium, Equine, Equine limb wound, Equine limb wounds, Equine limbs, Equine skin, Excisional wounds, Exuberant granulation tissue, Exuberant granulation tissue formation, Granulation, Granulation tissue, Greater quantities, Growth factor, Growth factors, Hair follicles, Hematoxylin, Hematoxylin gill, Immunohistochemical, Immunohistochemical localization, Immunoreactivity, Intense staining, Limb, Limb wounds, Localization, Macrophage, Negative control, Normal equine skin, Normal horse serum, Normal skin, Plenum press, Positive cytoplasmic staining, Primary antibody, Proliferative phase, Regulatory role, Repair process, Sebaceous glands, Second intention wound healing, Sigma diagnostics, Spatial expression, Stratum corneum, Strong staining, Thoracic, Thoracic wounds, Vector laboratories, Veterinary medicine, Veterinary surgeons, Western college, Wound base, Wound healing, Wound margins, Wound repair, Wound repair epidermis.
Abstract
Objective— To describe the localization of immunoreactive transforming growth factor (TGF)‐β1 in both normal skin and full‐thickness dermal wounds of the limb and the thorax of the horse.
Study design— Six full‐thickness excisional wounds were created on the lateral aspect of one metacarpal region and on the midthoracic area of each horse. Sequentially collected tissue specimens from wound margins were assessed for TGF‐β1 expression by immunohistochemistry.
Animals— Four horses (2 to 4 years of age).
Methods— A neutralizing monoclonal anti‐human TGF‐β1 antibody was used to detect the spatial expression of TGF‐β1 protein by immunohistochemical localization in biopsies obtained before wounding and at 12 and 24 hours, and 5, 10, and 14 days.
Results— No differences in localization of immunoreactive TGF‐β1 were detected between limb and thorax, for either intact skin or wounds. Unwounded epidermis stained moderately for TGF‐β1 protein throughout all layers, whereas the dermis was relatively devoid of immunoreactivity. During the acute stage of repair, migrating epithelium lost its stain, whereas cells of epidermal appendages remained strongly immunoreactive. The epithelium recovered its TGF‐β1 immunoreactivity during wound remodeling, although cells of the stratum corneum remained negative. Macrophages of the inflammatory exudate had positive cytoplasmic staining that diminished with time. Immunoreactivity of granulation tissue fibroblasts was evident early on and increased throughout the repair process.
Conclusions— TGF‐β1 is constitutively expressed in normal, unwounded equine epithelium. Its expression is upregulated within the skin on injury and is associated with the cells involved in wound repair.
Clinical relevance— A more precise understanding of the temporal and spatial expression of TGF‐β1 during wound repair in horses should provide the groundwork for possible future manipulations of both normal and aberrant tissue repair.
Url:
DOI: 10.1053/jvet.2002.32397
Affiliations:
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Theoret</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>From the Departments of Large Animal Clinical Sciences and *Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan</wicri:regionArea><wicri:noRegion>Saskatchewan</wicri:noRegion></affiliation></author><author><name sortKey="Barber, Spencer M" sort="Barber, Spencer M" uniqKey="Barber S" first="Spencer M." last="Barber">Spencer M. Barber</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>From the Departments of Large Animal Clinical Sciences and *Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan</wicri:regionArea><wicri:noRegion>Saskatchewan</wicri:noRegion></affiliation></author><author><name sortKey="Gordon, John R" sort="Gordon, John R" uniqKey="Gordon J" first="John R." last="Gordon">John R. Gordon</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>From the Departments of Large Animal Clinical Sciences and *Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan</wicri:regionArea><wicri:noRegion>Saskatchewan</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Veterinary Surgery</title><idno type="ISSN">0161-3499</idno><idno type="eISSN">1532-950X</idno><imprint><publisher>Blackwell Science Inc</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="2002-05">2002-05</date><biblScope unit="volume">31</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="274">274</biblScope><biblScope unit="page" to="280">280</biblScope></imprint><idno type="ISSN">0161-3499</idno></series><idno type="istex">695861745ADB56FA726E8765A7C71266AC03C5A3</idno><idno type="DOI">10.1053/jvet.2002.32397</idno><idno type="ArticleID">VSU274</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0161-3499</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aberrant tissue repair</term><term>Acute phase</term><term>American college</term><term>Appendage</term><term>Assoc equine practnr</term><term>Biopsy</term><term>Broblast growth factor</term><term>Broblasts</term><term>Brogenic growth factor</term><term>Cellular biology</term><term>Cellular components</term><term>Cutaneous epithelium</term><term>Diplomate acvs</term><term>Distal aspect</term><term>Epidermal</term><term>Epidermal appendages</term><term>Epidermal layers</term><term>Epithelial</term><term>Epithelial tips</term><term>Epithelium</term><term>Equine</term><term>Equine limb wound</term><term>Equine limb wounds</term><term>Equine limbs</term><term>Equine skin</term><term>Excisional wounds</term><term>Exuberant granulation tissue</term><term>Exuberant granulation tissue formation</term><term>Granulation</term><term>Granulation tissue</term><term>Greater quantities</term><term>Growth factor</term><term>Growth factors</term><term>Hair follicles</term><term>Hematoxylin</term><term>Hematoxylin gill</term><term>Immunohistochemical</term><term>Immunohistochemical localization</term><term>Immunoreactivity</term><term>Intense staining</term><term>Limb</term><term>Limb wounds</term><term>Localization</term><term>Macrophage</term><term>Negative control</term><term>Normal equine skin</term><term>Normal horse serum</term><term>Normal skin</term><term>Plenum press</term><term>Positive cytoplasmic staining</term><term>Primary antibody</term><term>Proliferative phase</term><term>Regulatory role</term><term>Repair process</term><term>Sebaceous glands</term><term>Second intention wound healing</term><term>Sigma diagnostics</term><term>Spatial expression</term><term>Stratum corneum</term><term>Strong staining</term><term>Thoracic</term><term>Thoracic wounds</term><term>Vector laboratories</term><term>Veterinary medicine</term><term>Veterinary surgeons</term><term>Western college</term><term>Wound base</term><term>Wound healing</term><term>Wound margins</term><term>Wound repair</term><term>Wound repair epidermis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Objective— To describe the localization of immunoreactive transforming growth factor (TGF)‐β1 in both normal skin and full‐thickness dermal wounds of the limb and the thorax of the horse.</div><div type="abstract">Study design— Six full‐thickness excisional wounds were created on the lateral aspect of one metacarpal region and on the midthoracic area of each horse. Sequentially collected tissue specimens from wound margins were assessed for TGF‐β1 expression by immunohistochemistry.</div><div type="abstract">Animals— Four horses (2 to 4 years of age).</div><div type="abstract">Methods— A neutralizing monoclonal anti‐human TGF‐β1 antibody was used to detect the spatial expression of TGF‐β1 protein by immunohistochemical localization in biopsies obtained before wounding and at 12 and 24 hours, and 5, 10, and 14 days.</div><div type="abstract">Results— No differences in localization of immunoreactive TGF‐β1 were detected between limb and thorax, for either intact skin or wounds. Unwounded epidermis stained moderately for TGF‐β1 protein throughout all layers, whereas the dermis was relatively devoid of immunoreactivity. During the acute stage of repair, migrating epithelium lost its stain, whereas cells of epidermal appendages remained strongly immunoreactive. The epithelium recovered its TGF‐β1 immunoreactivity during wound remodeling, although cells of the stratum corneum remained negative. Macrophages of the inflammatory exudate had positive cytoplasmic staining that diminished with time. Immunoreactivity of granulation tissue fibroblasts was evident early on and increased throughout the repair process.</div><div type="abstract">Conclusions— TGF‐β1 is constitutively expressed in normal, unwounded equine epithelium. Its expression is upregulated within the skin on injury and is associated with the cells involved in wound repair.</div><div type="abstract">Clinical relevance— A more precise understanding of the temporal and spatial expression of TGF‐β1 during wound repair in horses should provide the groundwork for possible future manipulations of both normal and aberrant tissue repair.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Theoret, Christine L" sort="Theoret, Christine L" uniqKey="Theoret C" first="Christine L." last="Theoret">Christine L. Theoret</name></noRegion><name sortKey="Barber, Spencer M" sort="Barber, Spencer M" uniqKey="Barber S" first="Spencer M." last="Barber">Spencer M. Barber</name><name sortKey="Gordon, John R" sort="Gordon, John R" uniqKey="Gordon J" first="John R." last="Gordon">John R. Gordon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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