Interrelationships of interleukin‐8 with interleukin‐1β and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women
Identifieur interne : 000639 ( Istex/Corpus ); précédent : 000638; suivant : 000640Interrelationships of interleukin‐8 with interleukin‐1β and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women
Auteurs : Sabina Cauci ; Secondo Guaschino ; Domenico De Aloysio ; Silvia Driussi ; Davide De Santo ; Paola Penacchioni ; Franco QuadrifoglioSource :
- Molecular Human Reproduction [ 1360-9947 ] ; 2003-01.
English descriptors
- KwdEn :
- Teeft :
- Bacterial vaginosis, Cauci, Chemokines, Cytokine, Eld, Eschenbach, Gynecol, Healthy controls, Healthy women, High concentrations, High levels, High neutrophil counts, High neutrophils, High number, High numbers, Hillier, Immune, Innate factors, Innate immunity, Leukocyte, Median, Median value, Microbial, Mucosal, Neutrophil, Neutrophil recruitment, Obstet, Percentile, Positive women, Quadrifoglio, Reprod, Subgroup, Such differences, Udine, Vaginal, Vaginal neutrophils, Vaginalis, Vaginosis.
Abstract
Vaginal innate immunity in response to microbial perturbation is still poorly understood and could be crucial for protection from adverse outcomes. We investigated the relationship between interleukin (IL)‐8, IL‐1β and neutrophils in vaginal fluid obtained from 60 healthy women and 51 women who were bacterial vaginosis (BV) positive. Concentrations of IL‐8 and IL‐1β were highly correlated with counts of neutrophils in vaginal fluid of the entire population examined (111 subjects). Vaginal IL‐1β concentrations were significantly higher (P < 0.001) in BV positive women. There was no significant difference in IL‐8 levels or number of neutrophils between healthy controls and BV positive women. None of the healthy controls with high neutrophil counts (≥75th percentile, 14 average count per field) had high concentrations of IL‐1β (≥75th percentile, 220 pg/ml), whereas 84% of BV positive women with high neutrophil counts had high IL‐1β concentrations (P < 0.001). On the contrary, no difference in the percentage of subjects with elevated concentrations of IL‐8 (≥75th percentile, 2842 pg/ml) was found between healthy and BV positive women with high numbers of neutrophils (55.5% of healthy versus 53% of BV positive women). Our findings show that BV causes a large increase in IL‐1β concentrations which is not paralleled by an increase in IL‐8 concentrations in vaginal fluid, suggesting that BV‐associated factors more specifically dampen IL‐8 rather than IL‐1β. The lack of an increase in IL‐8 may explain the absence of an increase in neutrophil numbers in most women exposed to abnormal vaginal colonization (BV).
Url:
DOI: 10.1093/molehr/gag003
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ISTEX:3CDA555FAA9725714F8C4A9958181E4ED93AFF10Le document en format XML
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Bologna,Via Massarenti, 40138 Bologna and</mods:affiliation></affiliation></author><author><name sortKey="Driussi, Silvia" sort="Driussi, Silvia" uniqKey="Driussi S" first="Silvia" last="Driussi">Silvia Driussi</name><affiliation><mods:affiliation>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</mods:affiliation></affiliation><affiliation><mods:affiliation>Azienda Servizi Sanitari N. 4 Medio Friuli, Via San Valentino, 33100 Udine, Italy</mods:affiliation></affiliation></author><author><name sortKey="De Santo, Davide" sort="De Santo, Davide" uniqKey="De Santo D" first="Davide" last="De Santo">Davide De Santo</name><affiliation><mods:affiliation>Obstetric and Gynecologic Unit, Department of Reproductive and Development Sciences, IRCCS Burlo Garofolo Hospital, School of Medicine, University of Trieste, Via dell’Istria 65/1, 34100 Trieste,</mods:affiliation></affiliation></author><author><name sortKey="Penacchioni, 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We investigated the relationship between interleukin (IL)‐8, IL‐1β and neutrophils in vaginal fluid obtained from 60 healthy women and 51 women who were bacterial vaginosis (BV) positive. Concentrations of IL‐8 and IL‐1β were highly correlated with counts of neutrophils in vaginal fluid of the entire population examined (111 subjects). Vaginal IL‐1β concentrations were significantly higher (P < 0.001) in BV positive women. There was no significant difference in IL‐8 levels or number of neutrophils between healthy controls and BV positive women. None of the healthy controls with high neutrophil counts (≥75th percentile, 14 average count per field) had high concentrations of IL‐1β (≥75th percentile, 220 pg/ml), whereas 84% of BV positive women with high neutrophil counts had high IL‐1β concentrations (P < 0.001). On the contrary, no difference in the percentage of subjects with elevated concentrations of IL‐8 (≥75th percentile, 2842 pg/ml) was found between healthy and BV positive women with high numbers of neutrophils (55.5% of healthy versus 53% of BV positive women). Our findings show that BV causes a large increase in IL‐1β concentrations which is not paralleled by an increase in IL‐8 concentrations in vaginal fluid, suggesting that BV‐associated factors more specifically dampen IL‐8 rather than IL‐1β. The lack of an increase in IL‐8 may explain the absence of an increase in neutrophil numbers in most women exposed to abnormal vaginal colonization (BV).</div></front></TEI><istex><corpusName>oup</corpusName><keywords><teeft><json:string>neutrophil</json:string><json:string>positive women</json:string><json:string>vaginosis</json:string><json:string>bacterial vaginosis</json:string><json:string>percentile</json:string><json:string>cauci</json:string><json:string>cytokine</json:string><json:string>subgroup</json:string><json:string>healthy controls</json:string><json:string>high concentrations</json:string><json:string>mucosal</json:string><json:string>obstet</json:string><json:string>gynecol</json:string><json:string>vaginalis</json:string><json:string>chemokines</json:string><json:string>eschenbach</json:string><json:string>hillier</json:string><json:string>leukocyte</json:string><json:string>immune</json:string><json:string>reprod</json:string><json:string>udine</json:string><json:string>quadrifoglio</json:string><json:string>eld</json:string><json:string>high neutrophil counts</json:string><json:string>healthy women</json:string><json:string>high levels</json:string><json:string>vaginal neutrophils</json:string><json:string>vaginal</json:string><json:string>median</json:string><json:string>microbial</json:string><json:string>median value</json:string><json:string>high neutrophils</json:string><json:string>such differences</json:string><json:string>high numbers</json:string><json:string>innate immunity</json:string><json:string>high number</json:string><json:string>neutrophil recruitment</json:string><json:string>innate factors</json:string></teeft></keywords><author><json:item><name>Sabina Cauci</name><affiliations><json:string>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</json:string><json:string>To whom correspondence should be addressed. e‐mail: scauci@mail.dstb.uniud.it</json:string></affiliations></json:item><json:item><name>Secondo Guaschino</name><affiliations><json:string>Obstetric and Gynecologic Unit, Department of Reproductive and Development Sciences, IRCCS Burlo Garofolo Hospital, School of Medicine, University of Trieste, Via dell’Istria 65/1, 34100 Trieste,</json:string></affiliations></json:item><json:item><name>Domenico de Aloysio</name><affiliations><json:string>Department of Obstetrics and Gynecology, Sant’Orsola Hospital, School of Medicine, University of Bologna,Via Massarenti, 40138 Bologna and</json:string></affiliations></json:item><json:item><name>Silvia Driussi</name><affiliations><json:string>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</json:string><json:string>Azienda Servizi Sanitari N. 4 Medio Friuli, Via San Valentino, 33100 Udine, Italy</json:string></affiliations></json:item><json:item><name>Davide De Santo</name><affiliations><json:string>Obstetric and Gynecologic Unit, Department of Reproductive and Development Sciences, IRCCS Burlo Garofolo Hospital, School of Medicine, University of Trieste, Via dell’Istria 65/1, 34100 Trieste,</json:string></affiliations></json:item><json:item><name>Paola Penacchioni</name><affiliations><json:string>Department of Obstetrics and Gynecology, Sant’Orsola Hospital, School of Medicine, University of Bologna,Via Massarenti, 40138 Bologna and</json:string></affiliations></json:item><json:item><name>Franco Quadrifoglio</name><affiliations><json:string>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Key words: bacterial vaginosis/interleukin‐1β/interleukin‐8/mucosal innate immunity/vaginal leukocytes</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>Vaginal innate immunity in response to microbial perturbation is still poorly understood and could be crucial for protection from adverse outcomes. We investigated the relationship between interleukin (IL)‐8, IL‐1β and neutrophils in vaginal fluid obtained from 60 healthy women and 51 women who were bacterial vaginosis (BV) positive. Concentrations of IL‐8 and IL‐1β were highly correlated with counts of neutrophils in vaginal fluid of the entire population examined (111 subjects). Vaginal IL‐1β concentrations were significantly higher (P > 0.001) in BV positive women. There was no significant difference in IL‐8 levels or number of neutrophils between healthy controls and BV positive women. None of the healthy controls with high neutrophil counts (≥75th percentile, 14 average count per field) had high concentrations of IL‐1β (≥75th percentile, 220 pg/ml), whereas 84% of BV positive women with high neutrophil counts had high IL‐1β concentrations (P > 0.001). On the contrary, no difference in the percentage of subjects with elevated concentrations of IL‐8 (≥75th percentile, 2842 pg/ml) was found between healthy and BV positive women with high numbers of neutrophils (55.5% of healthy versus 53% of BV positive women). Our findings show that BV causes a large increase in IL‐1β concentrations which is not paralleled by an increase in IL‐8 concentrations in vaginal fluid, suggesting that BV‐associated factors more specifically dampen IL‐8 rather than IL‐1β. The lack of an increase in IL‐8 may explain the absence of an increase in neutrophil numbers in most women exposed to abnormal vaginal colonization (BV).</abstract><qualityIndicators><score>9.394</score><pdfVersion>1.2</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>1</keywordCount><abstractCharCount>1627</abstractCharCount><pdfWordCount>4430</pdfWordCount><pdfCharCount>28850</pdfCharCount><pdfPageCount>6</pdfPageCount><abstractWordCount>247</abstractWordCount></qualityIndicators><title>Interrelationships of interleukin‐8 with interleukin‐1β and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women</title><genre><json:string>research-article</json:string></genre><host><title>Molecular Human Reproduction</title><language><json:string>unknown</json:string></language><issn><json:string>1360-9947</json:string></issn><eissn><json:string>1460-2407</json:string></eissn><publisherId><json:string>molehr</json:string></publisherId><volume>9</volume><issue>1</issue><pages><first>53</first><last>58</last></pages><genre><json:string>journal</json:string></genre></host><categories><wos><json:string>science</json:string><json:string>reproductive biology</json:string><json:string>obstetrics & gynecology</json:string><json:string>developmental biology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>clinical medicine</json:string><json:string>obstetrics & reproductive medicine</json:string></scienceMetrix><inist><json:string>sciences appliquees, technologies et medecines</json:string><json:string>sciences biologiques et medicales</json:string><json:string>sciences medicales</json:string></inist></categories><publicationDate>2003</publicationDate><copyrightDate>2003</copyrightDate><doi><json:string>10.1093/molehr/gag003</json:string></doi><id>3CDA555FAA9725714F8C4A9958181E4ED93AFF10</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/3CDA555FAA9725714F8C4A9958181E4ED93AFF10/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/3CDA555FAA9725714F8C4A9958181E4ED93AFF10/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/3CDA555FAA9725714F8C4A9958181E4ED93AFF10/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Interrelationships of interleukin‐8 with interleukin‐1β and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Oxford University Press</publisher><availability><p>OUP</p></availability><date>2003</date></publicationStmt><notesStmt><note>Submitted on July 11, 2002; accepted on October 15, 2002</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Interrelationships of interleukin‐8 with interleukin‐1β and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women</title><author xml:id="author-0000"><persName><forename type="first">Sabina</forename><surname>Cauci</surname></persName><affiliation>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</affiliation><affiliation>To whom correspondence should be addressed. e‐mail: scauci@mail.dstb.uniud.it</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Secondo</forename><surname>Guaschino</surname></persName><affiliation>Obstetric and Gynecologic Unit, Department of Reproductive and Development Sciences, IRCCS Burlo Garofolo Hospital, School of Medicine, University of Trieste, Via dell’Istria 65/1, 34100 Trieste,</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Domenico</forename><surname>de Aloysio</surname></persName><affiliation>Department of Obstetrics and Gynecology, Sant’Orsola Hospital, School of Medicine, University of Bologna,Via Massarenti, 40138 Bologna and</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Silvia</forename><surname>Driussi</surname></persName><affiliation>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</affiliation><affiliation>Azienda Servizi Sanitari N. 4 Medio Friuli, Via San Valentino, 33100 Udine, Italy</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Davide</forename><surname>De Santo</surname></persName><affiliation>Obstetric and Gynecologic Unit, Department of Reproductive and Development Sciences, IRCCS Burlo Garofolo Hospital, School of Medicine, University of Trieste, Via dell’Istria 65/1, 34100 Trieste,</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Paola</forename><surname>Penacchioni</surname></persName><affiliation>Department of Obstetrics and Gynecology, Sant’Orsola Hospital, School of Medicine, University of Bologna,Via Massarenti, 40138 Bologna and</affiliation></author><author xml:id="author-0006"><persName><forename type="first">Franco</forename><surname>Quadrifoglio</surname></persName><affiliation>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</affiliation></author><idno type="istex">3CDA555FAA9725714F8C4A9958181E4ED93AFF10</idno><idno type="DOI">10.1093/molehr/gag003</idno><idno type="local">gag003</idno></analytic><monogr><title level="j">Molecular Human Reproduction</title><title level="j" type="abbrev">Mol. Hum. Reprod.</title><idno type="pISSN">1360-9947</idno><idno type="eISSN">1460-2407</idno><idno type="PublisherID">molehr</idno><idno type="PublisherID-hwp">molehr</idno><idno type="PublisherID-nlm-ta">Mol Hum Reprod</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2003-01"></date><biblScope unit="volume">9</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="53">53</biblScope><biblScope unit="page" to="58">58</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2003</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Vaginal innate immunity in response to microbial perturbation is still poorly understood and could be crucial for protection from adverse outcomes. We investigated the relationship between interleukin (IL)‐8, IL‐1β and neutrophils in vaginal fluid obtained from 60 healthy women and 51 women who were bacterial vaginosis (BV) positive. Concentrations of IL‐8 and IL‐1β were highly correlated with counts of neutrophils in vaginal fluid of the entire population examined (111 subjects). Vaginal IL‐1β concentrations were significantly higher (P < 0.001) in BV positive women. There was no significant difference in IL‐8 levels or number of neutrophils between healthy controls and BV positive women. None of the healthy controls with high neutrophil counts (≥75th percentile, 14 average count per field) had high concentrations of IL‐1β (≥75th percentile, 220 pg/ml), whereas 84% of BV positive women with high neutrophil counts had high IL‐1β concentrations (P < 0.001). On the contrary, no difference in the percentage of subjects with elevated concentrations of IL‐8 (≥75th percentile, 2842 pg/ml) was found between healthy and BV positive women with high numbers of neutrophils (55.5% of healthy versus 53% of BV positive women). Our findings show that BV causes a large increase in IL‐1β concentrations which is not paralleled by an increase in IL‐8 concentrations in vaginal fluid, suggesting that BV‐associated factors more specifically dampen IL‐8 rather than IL‐1β. The lack of an increase in IL‐8 may explain the absence of an increase in neutrophil numbers in most women exposed to abnormal vaginal colonization (BV).</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>KWD</head><item><term>Key words: bacterial vaginosis/interleukin‐1β/interleukin‐8/mucosal innate immunity/vaginal leukocytes</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2003-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/3CDA555FAA9725714F8C4A9958181E4ED93AFF10/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="US-ASCII"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article xml:lang="en" article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">molehr</journal-id><journal-id journal-id-type="nlm-ta">Mol Hum Reprod</journal-id><journal-id journal-id-type="publisher-id">molehr</journal-id><journal-title>Molecular Human Reproduction</journal-title><abbrev-journal-title abbrev-type="publisher">Mol. Hum. Reprod.</abbrev-journal-title><issn pub-type="ppub">1360-9947</issn><issn pub-type="epub">1460-2407</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="other">gag003</article-id><article-id pub-id-type="doi">10.1093/molehr/gag003</article-id><article-categories><subj-group subj-group-type="heading"><subject>Immunology of reproduction</subject></subj-group></article-categories><title-group><article-title>Interrelationships of interleukin‐8 with interleukin‐1β and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Cauci</surname><given-names>Sabina</given-names></name><xref rid="GAG003A1">1</xref><xref rid="GAG003A5">5</xref></contrib><contrib contrib-type="author"><name><surname>Guaschino</surname><given-names>Secondo</given-names></name><xref rid="GAG003A2">2</xref></contrib><contrib contrib-type="author"><name><surname>de Aloysio</surname><given-names>Domenico</given-names></name><xref rid="GAG003A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Driussi</surname><given-names>Silvia</given-names></name><xref rid="GAG003A1">1</xref><xref rid="GAG003A4">4</xref></contrib><contrib contrib-type="author"><name><surname>De Santo</surname><given-names>Davide</given-names></name><xref rid="GAG003A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Penacchioni</surname><given-names>Paola</given-names></name><xref rid="GAG003A3">3</xref></contrib><contrib contrib-type="author"><name><surname>Quadrifoglio</surname><given-names>Franco</given-names></name><xref rid="GAG003A1">1</xref></contrib><aff id="GAG003A1"><label>1</label>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine, </aff><aff id="GAG003A2"><label>2</label>Obstetric and Gynecologic Unit, Department of Reproductive and Development Sciences, IRCCS Burlo Garofolo Hospital, School of Medicine, University of Trieste, Via dell’Istria 65/1, 34100 Trieste, </aff><aff id="GAG003A3"><label>3</label>Department of Obstetrics and Gynecology, Sant’Orsola Hospital, School of Medicine, University of Bologna,Via Massarenti, 40138 Bologna and </aff><aff id="GAG003A4"><label>4</label>Azienda Servizi Sanitari N. 4 Medio Friuli, Via San Valentino, 33100 Udine, Italy</aff><aff id="GAG003A5"><label>5</label>To whom correspondence should be addressed. e‐mail: <ext-link xlink:href="scauci@mail.dstb.uniud.it" ext-link-type="email">scauci@mail.dstb.uniud.it</ext-link></aff></contrib-group><pub-date pub-type="ppub"><month>01</month><year>2003</year></pub-date><volume>9</volume><issue>1</issue><fpage>53</fpage><lpage>58</lpage><permissions><copyright-year>2003</copyright-year></permissions><abstract xml:lang="en"><p>Vaginal innate immunity in response to microbial perturbation is still poorly understood and could be crucial for protection from adverse outcomes. We investigated the relationship between interleukin (IL)‐8, IL‐1β and neutrophils in vaginal fluid obtained from 60 healthy women and 51 women who were bacterial vaginosis (BV) positive. Concentrations of IL‐8 and IL‐1β were highly correlated with counts of neutrophils in vaginal fluid of the entire population examined (111 subjects). Vaginal IL‐1β concentrations were significantly higher (<italic>P</italic> < 0.001) in BV positive women. There was no significant difference in IL‐8 levels or number of neutrophils between healthy controls and BV positive women. None of the healthy controls with high neutrophil counts (≥75th percentile, 14 average count per field) had high concentrations of IL‐1β (≥75th percentile, 220 pg/ml), whereas 84% of BV positive women with high neutrophil counts had high IL‐1β concentrations (<italic>P</italic> < 0.001). On the contrary, no difference in the percentage of subjects with elevated concentrations of IL‐8 (≥75th percentile, 2842 pg/ml) was found between healthy and BV positive women with high numbers of neutrophils (55.5% of healthy versus 53% of BV positive women). Our findings show that BV causes a large increase in IL‐1β concentrations which is not paralleled by an increase in IL‐8 concentrations in vaginal fluid, suggesting that BV‐associated factors more specifically dampen IL‐8 rather than IL‐1β. The lack of an increase in IL‐8 may explain the absence of an increase in neutrophil numbers in most women exposed to abnormal vaginal colonization (BV).</p></abstract><kwd-group kwd-group-type="KWD" xml:lang="en"><kwd><italic>Key words:</italic> bacterial vaginosis/interleukin‐1β/interleukin‐8/mucosal innate immunity/vaginal leukocytes</kwd></kwd-group><custom-meta-wrap><custom-meta><meta-name>hwp-legacy-fpage</meta-name><meta-value>53</meta-value></custom-meta><custom-meta><meta-name>hwp-legacy-dochead</meta-name><meta-value>Article</meta-value></custom-meta></custom-meta-wrap></article-meta><notes><p content-type="arthw-misc">Submitted on July 11, 2002; accepted on October 15, 2002</p></notes></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Interrelationships of interleukin‐8 with interleukin‐1β and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Interrelationships of interleukin‐8 with interleukin‐1β and neutrophils in vaginal fluid of healthy and bacterial vaginosis positive women</title></titleInfo><name type="personal"><namePart type="given">Sabina</namePart><namePart type="family">Cauci</namePart><affiliation>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</affiliation><affiliation>To whom correspondence should be addressed. e‐mail: scauci@mail.dstb.uniud.it</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Secondo</namePart><namePart type="family">Guaschino</namePart><affiliation>Obstetric and Gynecologic Unit, Department of Reproductive and Development Sciences, IRCCS Burlo Garofolo Hospital, School of Medicine, University of Trieste, Via dell’Istria 65/1, 34100 Trieste,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Domenico</namePart><namePart type="family">de Aloysio</namePart><affiliation>Department of Obstetrics and Gynecology, Sant’Orsola Hospital, School of Medicine, University of Bologna,Via Massarenti, 40138 Bologna and</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Silvia</namePart><namePart type="family">Driussi</namePart><affiliation>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</affiliation><affiliation>Azienda Servizi Sanitari N. 4 Medio Friuli, Via San Valentino, 33100 Udine, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Davide</namePart><namePart type="family">De Santo</namePart><affiliation>Obstetric and Gynecologic Unit, Department of Reproductive and Development Sciences, IRCCS Burlo Garofolo Hospital, School of Medicine, University of Trieste, Via dell’Istria 65/1, 34100 Trieste,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Paola</namePart><namePart type="family">Penacchioni</namePart><affiliation>Department of Obstetrics and Gynecology, Sant’Orsola Hospital, School of Medicine, University of Bologna,Via Massarenti, 40138 Bologna and</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Franco</namePart><namePart type="family">Quadrifoglio</namePart><affiliation>Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Piazzale Kolbe 4, 33100 Udine,</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2003-01</dateIssued><copyrightDate encoding="w3cdtf">2003</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Vaginal innate immunity in response to microbial perturbation is still poorly understood and could be crucial for protection from adverse outcomes. We investigated the relationship between interleukin (IL)‐8, IL‐1β and neutrophils in vaginal fluid obtained from 60 healthy women and 51 women who were bacterial vaginosis (BV) positive. Concentrations of IL‐8 and IL‐1β were highly correlated with counts of neutrophils in vaginal fluid of the entire population examined (111 subjects). Vaginal IL‐1β concentrations were significantly higher (P < 0.001) in BV positive women. There was no significant difference in IL‐8 levels or number of neutrophils between healthy controls and BV positive women. None of the healthy controls with high neutrophil counts (≥75th percentile, 14 average count per field) had high concentrations of IL‐1β (≥75th percentile, 220 pg/ml), whereas 84% of BV positive women with high neutrophil counts had high IL‐1β concentrations (P < 0.001). On the contrary, no difference in the percentage of subjects with elevated concentrations of IL‐8 (≥75th percentile, 2842 pg/ml) was found between healthy and BV positive women with high numbers of neutrophils (55.5% of healthy versus 53% of BV positive women). Our findings show that BV causes a large increase in IL‐1β concentrations which is not paralleled by an increase in IL‐8 concentrations in vaginal fluid, suggesting that BV‐associated factors more specifically dampen IL‐8 rather than IL‐1β. The lack of an increase in IL‐8 may explain the absence of an increase in neutrophil numbers in most women exposed to abnormal vaginal colonization (BV).</abstract><note>Submitted on July 11, 2002; accepted on October 15, 2002</note><subject lang="en"><genre>KWD</genre><topic>Key words: bacterial vaginosis/interleukin‐1β/interleukin‐8/mucosal innate immunity/vaginal leukocytes</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Human Reproduction</title></titleInfo><titleInfo type="abbreviated"><title>Mol. Hum. Reprod.</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1360-9947</identifier><identifier type="eISSN">1460-2407</identifier><identifier type="PublisherID">molehr</identifier><identifier type="PublisherID-hwp">molehr</identifier><identifier type="PublisherID-nlm-ta">Mol Hum Reprod</identifier><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>9</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>53</start><end>58</end></extent></part></relatedItem><identifier type="istex">3CDA555FAA9725714F8C4A9958181E4ED93AFF10</identifier><identifier type="DOI">10.1093/molehr/gag003</identifier><identifier type="local">gag003</identifier><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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