Temperature experiments on nerve and muscle membranes of frogs
Identifieur interne : 000292 ( Istex/Corpus ); précédent : 000291; suivant : 000293Temperature experiments on nerve and muscle membranes of frogs
Auteurs : Wolfgang SchwarzSource :
- Pflügers Archiv [ 0031-6768 ] ; 1979-10-01.
English descriptors
- Teeft :
- Absolute rate theory, Activation energy, Activation enthalpies, Activation enthalpy, Anaesthetic, Anaesthetic benzocaine, Arrhenius, Arrhenius plot, Arrhenius plots, Benzocaine, Cell membranes, Channel gating, Channel protein, Conductance, Critical value, Depolarizing, Depolarizing pulse, Different temperatures, Divalent cations, Drug molecules, Enthalpy, Excitable membranes, External potassium, External solution, Fibre, Frankenhaeuser, Frog, Frog nerve, Gating, Higher temperatures, Hille, Hysteresis, Hysteresis width, Inactivation, Integral proteins, Internal solution, Kinetics, Lipid, Lipid phase, Lipid phase transition, Local anaesthetics, Locust muscle, Lond, Lower temperatures, Membrane, Membrane lipids, Muscle fiber, Muscle fibers, Muscle membranes, Muscle preparations, Myelinated, Myelinated nerve, Myelinated nerve fibers, Myelinated nerve fibres, Node, Other hand, Peak currents, Pflfigers arch, Pfliigers arch, Pfltigers arch, Phase change, Phase transition, Phase transitions, Physiol, Quantitative description, Ranvier, Ranvier nodes, Rate constants, Receptor, Same computer, Same temperature, Schwarz, Slow changes, Slow inactivation, Slow sodium inactivation, Sodium channels, Sodium currents, Solid lines, Standardized pulses, Straight lines, Subsequent heating, Temperature change, Temperature changes, Temperature dependence, Temperature experiments, Temperature jump experiments, Temperature range, Time constants, Time course, Transition temperature, Transition temperatures, Voltage dependence, Xenopus laevis.
Abstract
Abstract: The influence of temperature changes in the range of 25°C to −6°C on the time constants of Na activation (τm) and inactivation (τh) was studied in twitch muscle fibers and the node of Ranvier under voltage-clamp conditions. Arrhenius plots of τm and τh exhibit a change in activation enthalpy at temperatures below 10°C. Cooling and subsequent heating induce a hystersis in the temperature dependence of τm and τh Ni2+ and UO 2 2+ increase the hysteresis width. With fast temperature changes the gating kinetics relax to their new values more slowly than the temperature change. Hence, temperature must be changed more slowly than 5°C/min if an additional apparent hysteresis due simply to this relaxation is to be avoided. The data are explained by the hypothesis of a phase transition in the membrane lipids. This conception is favoured over a temperature-induced change in protein conformation, since the neutral local anaesthetic benzocaine shows use-dependent block as if low temperature restricted the access of the drug through the lipid phase to its receptor.
Url:
DOI: 10.1007/BF00585900
Links to Exploration step
ISTEX:1C3A333979C1C574E7EDC8ADD24EDDEFFE89CC2ALe document en format XML
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Arrhenius plots of τm and τh exhibit a change in activation enthalpy at temperatures below 10°C. Cooling and subsequent heating induce a hystersis in the temperature dependence of τm and τh Ni2+ and UO 2 2+ increase the hysteresis width. With fast temperature changes the gating kinetics relax to their new values more slowly than the temperature change. Hence, temperature must be changed more slowly than 5°C/min if an additional apparent hysteresis due simply to this relaxation is to be avoided. The data are explained by the hypothesis of a phase transition in the membrane lipids. 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Physiologisches Institut, Universität des Saarlandes, D-6650, Homburg (Saar), Federal Republic of Germany</json:string></affiliations></json:item></author><articleId><json:string>BF00585900</json:string><json:string>Art4</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Abstract: The influence of temperature changes in the range of 25°C to −6°C on the time constants of Na activation (τm) and inactivation (τh) was studied in twitch muscle fibers and the node of Ranvier under voltage-clamp conditions. Arrhenius plots of τm and τh exhibit a change in activation enthalpy at temperatures below 10°C. Cooling and subsequent heating induce a hystersis in the temperature dependence of τm and τh Ni2+ and UO 2 2+ increase the hysteresis width. With fast temperature changes the gating kinetics relax to their new values more slowly than the temperature change. Hence, temperature must be changed more slowly than 5°C/min if an additional apparent hysteresis due simply to this relaxation is to be avoided. The data are explained by the hypothesis of a phase transition in the membrane lipids. This conception is favoured over a temperature-induced change in protein conformation, since the neutral local anaesthetic benzocaine shows use-dependent block as if low temperature restricted the access of the drug through the lipid phase to its receptor.</abstract><qualityIndicators><score>7.052</score><pdfWordCount>5409</pdfWordCount><pdfCharCount>30775</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>594 x 792 pts</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>171</abstractWordCount><abstractCharCount>1077</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Temperature experiments on nerve and muscle membranes of frogs</title><genre><json:string>research-article</json:string></genre><host><title>Pflügers Archiv</title><language><json:string>unknown</json:string></language><publicationDate>1979</publicationDate><copyrightDate>1979</copyrightDate><issn><json:string>0031-6768</json:string></issn><eissn><json:string>1432-2013</json:string></eissn><journalId><json:string>424</json:string></journalId><volume>382</volume><issue>1</issue><pages><first>27</first><last>34</last></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Human Physiology</value></json:item></subject></host><categories><wos><json:string>science</json:string><json:string>physiology</json:string></wos><scienceMetrix><json:string>health sciences</json:string><json:string>biomedical research</json:string><json:string>physiology</json:string></scienceMetrix><inist><json:string>sciences appliquees, technologies et medecines</json:string><json:string>sciences biologiques et medicales</json:string><json:string>sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>psychologie. psychophysiologie</json:string></inist></categories><publicationDate>1979</publicationDate><copyrightDate>1979</copyrightDate><doi><json:string>10.1007/BF00585900</json:string></doi><id>1C3A333979C1C574E7EDC8ADD24EDDEFFE89CC2A</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/1C3A333979C1C574E7EDC8ADD24EDDEFFE89CC2A/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/1C3A333979C1C574E7EDC8ADD24EDDEFFE89CC2A/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/1C3A333979C1C574E7EDC8ADD24EDDEFFE89CC2A/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Temperature experiments on nerve and muscle membranes of frogs</title><title level="a" type="sub" xml:lang="en">Indications for a phase transition</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Springer-Verlag</publisher><pubPlace>Berlin/Heidelberg</pubPlace><availability><p>Springer-Verlag, 1979</p></availability><date>1979-02-09</date></publicationStmt><notesStmt><note>Excitable Tissues and Central Nervous Physiology</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Temperature experiments on nerve and muscle membranes of frogs</title><title level="a" type="sub" xml:lang="en">Indications for a phase transition</title><author xml:id="author-0000" corresp="yes"><persName><forename type="first">Wolfgang</forename><surname>Schwarz</surname></persName><affiliation>Dept of Physiology and Biophysics, University of Washington, 98195, Seattle, WA, USA</affiliation><affiliation>I. 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Arrhenius plots of τm and τh exhibit a change in activation enthalpy at temperatures below 10°C. Cooling and subsequent heating induce a hystersis in the temperature dependence of τm and τh Ni2+ and UO 2 2+ increase the hysteresis width. With fast temperature changes the gating kinetics relax to their new values more slowly than the temperature change. Hence, temperature must be changed more slowly than 5°C/min if an additional apparent hysteresis due simply to this relaxation is to be avoided. The data are explained by the hypothesis of a phase transition in the membrane lipids. This conception is favoured over a temperature-induced change in protein conformation, since the neutral local anaesthetic benzocaine shows use-dependent block as if low temperature restricted the access of the drug through the lipid phase to its receptor.</p></abstract><textClass><keywords scheme="Journal Subject"><list><head>Biomedicine</head><item><term>Human Physiology</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1979-02-09">Created</change><change when="1979-10-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/1C3A333979C1C574E7EDC8ADD24EDDEFFE89CC2A/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Springer, Publisher found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Springer-Verlag</PublisherName><PublisherLocation>Berlin/Heidelberg</PublisherLocation></PublisherInfo><Journal><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>424</JournalID><JournalPrintISSN>0031-6768</JournalPrintISSN><JournalElectronicISSN>1432-2013</JournalElectronicISSN><JournalTitle>Pflügers Archiv</JournalTitle><JournalSubTitle>European Journal of Physiology</JournalSubTitle><JournalAbbreviatedTitle>Pflugers Arch.</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Type="Primary">Biomedicine</JournalSubject><JournalSubject Type="Secondary">Human Physiology</JournalSubject></JournalSubjectGroup></JournalInfo><Volume><VolumeInfo VolumeType="Regular" TocLevels="0"><VolumeIDStart>382</VolumeIDStart><VolumeIDEnd>382</VolumeIDEnd><VolumeIssueCount>3</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular"><IssueInfo TocLevels="0"><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd><IssueArticleCount>16</IssueArticleCount><IssueHistory><CoverDate><Year>1979</Year><Month>10</Month></CoverDate></IssueHistory><IssueCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1979</CopyrightYear></IssueCopyright></IssueInfo><Article ID="Art4"><ArticleInfo Language="En" ArticleType="OriginalPaper" NumberingStyle="Unnumbered" TocLevels="0" ContainsESM="No"><ArticleID>BF00585900</ArticleID><ArticleDOI>10.1007/BF00585900</ArticleDOI><ArticleSequenceNumber>4</ArticleSequenceNumber><ArticleTitle Language="En">Temperature experiments on nerve and muscle membranes of frogs</ArticleTitle><ArticleSubTitle Language="En">Indications for a phase transition</ArticleSubTitle><ArticleCategory>Excitable Tissues and Central Nervous Physiology</ArticleCategory><ArticleFirstPage>27</ArticleFirstPage><ArticleLastPage>34</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2004</Year><Month>10</Month><Day>21</Day></RegistrationDate><Received><Year>1979</Year><Month>2</Month><Day>9</Day></Received><Accepted><Year>1979</Year><Month>7</Month><Day>23</Day></Accepted></ArticleHistory><ArticleCopyright><CopyrightHolderName>Springer-Verlag</CopyrightHolderName><CopyrightYear>1979</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants><ArticleContext><JournalID>424</JournalID><VolumeIDStart>382</VolumeIDStart><VolumeIDEnd>382</VolumeIDEnd><IssueIDStart>1</IssueIDStart><IssueIDEnd>1</IssueIDEnd></ArticleContext></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1 Aff2" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Wolfgang</GivenName><FamilyName>Schwarz</FamilyName></AuthorName></Author><Affiliation ID="Aff1"><OrgDivision>Dept of Physiology and Biophysics</OrgDivision><OrgName>University of Washington</OrgName><OrgAddress><Postcode>98195</Postcode><City>Seattle</City><State>WA</State><Country>USA</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>I. Physiologisches Institut</OrgDivision><OrgName>Universität des Saarlandes</OrgName><OrgAddress><Postcode>D-6650</Postcode><City>Homburg (Saar)</City><Country>Federal Republic of Germany</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Abstract</Heading><Para>The influence of temperature changes in the range of 25°C to −6°C on the time constants of Na activation (τ<Emphasis Type="Italic">m</Emphasis>) and inactivation (τ<Emphasis Type="Italic">h</Emphasis>) was studied in twitch muscle fibers and the node of Ranvier under voltage-clamp conditions. Arrhenius plots of τ<Emphasis Type="Italic">m</Emphasis> and τ<Emphasis Type="Italic">h</Emphasis> exhibit a change in activation enthalpy at temperatures below 10°C. Cooling and subsequent heating induce a hystersis in the temperature dependence of τ<Emphasis Type="Italic">m</Emphasis> and τ<Emphasis Type="Italic">h</Emphasis> Ni<Superscript>2+</Superscript> and UO<Stack><Subscript>2</Subscript><Superscript>2+</Superscript></Stack> increase the hysteresis width. With fast temperature changes the gating kinetics relax to their new values more slowly than the temperature change. Hence, temperature must be changed more slowly than 5°C/min if an additional apparent hysteresis due simply to this relaxation is to be avoided. The data are explained by the hypothesis of a phase transition in the membrane lipids. This conception is favoured over a temperature-induced change in protein conformation, since the neutral local anaesthetic benzocaine shows use-dependent block as if low temperature restricted the access of the drug through the lipid phase to its receptor.</Para></Abstract><KeywordGroup Language="En"><Heading>Key words</Heading><Keyword>Voltage clamp</Keyword><Keyword>Node of Ranvier</Keyword><Keyword>Twitch muscle fiber</Keyword><Keyword>Na current</Keyword><Keyword>Effects of temperature</Keyword></KeywordGroup><ArticleNote Type="Misc"><SimplePara>Supported by grant NS 08174 from the U.S. Public Health Service and SFB 38 from Deutsche Forschungsgemeinschaft</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Temperature experiments on nerve and muscle membranes of frogs</title><subTitle>Indications for a phase transition</subTitle></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Temperature experiments on nerve and muscle membranes of frogs</title><subTitle>Indications for a phase transition</subTitle></titleInfo><name type="personal" displayLabel="corresp"><namePart type="given">Wolfgang</namePart><namePart type="family">Schwarz</namePart><affiliation>Dept of Physiology and Biophysics, University of Washington, 98195, Seattle, WA, USA</affiliation><affiliation>I. Physiologisches Institut, Universität des Saarlandes, D-6650, Homburg (Saar), Federal Republic of Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper"></genre><originInfo><publisher>Springer-Verlag</publisher><place><placeTerm type="text">Berlin/Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">1979-02-09</dateCreated><dateIssued encoding="w3cdtf">1979-10-01</dateIssued><copyrightDate encoding="w3cdtf">1979</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">Abstract: The influence of temperature changes in the range of 25°C to −6°C on the time constants of Na activation (τm) and inactivation (τh) was studied in twitch muscle fibers and the node of Ranvier under voltage-clamp conditions. Arrhenius plots of τm and τh exhibit a change in activation enthalpy at temperatures below 10°C. Cooling and subsequent heating induce a hystersis in the temperature dependence of τm and τh Ni2+ and UO 2 2+ increase the hysteresis width. With fast temperature changes the gating kinetics relax to their new values more slowly than the temperature change. Hence, temperature must be changed more slowly than 5°C/min if an additional apparent hysteresis due simply to this relaxation is to be avoided. The data are explained by the hypothesis of a phase transition in the membrane lipids. This conception is favoured over a temperature-induced change in protein conformation, since the neutral local anaesthetic benzocaine shows use-dependent block as if low temperature restricted the access of the drug through the lipid phase to its receptor.</abstract><note>Excitable Tissues and Central Nervous Physiology</note><relatedItem type="host"><titleInfo><title>Pflügers Archiv</title><subTitle>European Journal of Physiology</subTitle></titleInfo><titleInfo type="abbreviated"><title>Pflugers Arch.</title></titleInfo><genre type="journal" displayLabel="Archive Journal"></genre><originInfo><dateIssued encoding="w3cdtf">1979-10-01</dateIssued><copyrightDate encoding="w3cdtf">1979</copyrightDate></originInfo><subject><genre>Biomedicine</genre><topic>Human Physiology</topic></subject><identifier type="ISSN">0031-6768</identifier><identifier type="eISSN">1432-2013</identifier><identifier type="JournalID">424</identifier><identifier type="IssueArticleCount">16</identifier><identifier type="VolumeIssueCount">3</identifier><part><date>1979</date><detail type="volume"><number>382</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="pages"><start>27</start><end>34</end></extent></part><recordInfo><recordOrigin>Springer-Verlag, 1979</recordOrigin></recordInfo></relatedItem><identifier type="istex">1C3A333979C1C574E7EDC8ADD24EDDEFFE89CC2A</identifier><identifier type="DOI">10.1007/BF00585900</identifier><identifier type="ArticleID">BF00585900</identifier><identifier type="ArticleID">Art4</identifier><accessCondition type="use and reproduction" contentType="copyright">Springer-Verlag, 1979</accessCondition><recordInfo><recordContentSource>SPRINGER</recordContentSource><recordOrigin>Springer-Verlag, 1979</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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