A rapid, sensitive and economic method for the detection, quantification and confirmation of aflatoxins.
Identifieur interne : 000A34 ( PubMed/Corpus ); précédent : 000A33; suivant : 000A35A rapid, sensitive and economic method for the detection, quantification and confirmation of aflatoxins.
Auteurs : P. Majerus ; Z. ZakariaSource :
- Zeitschrift fur Lebensmittel-Untersuchung und -Forschung [ 0044-3026 ] ; 1992.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Aflatoxins.
- Arachis, Chromatography, Thin Layer, Cottonseed Oil, Food Microbiology, Sensitivity and Specificity, Zea mays.
Abstract
A rapid, sensitive and economic method for the detection, quantification and confirmation of aflatoxins is described. Aflatoxins B1, B2, G1, and G2, are extracted by methanol/water (85 + 15) and partitioned into methylene dichloride. The methylene dichloride solution is cleaned up on a polypropylene column, filled with 0.5 g silica gel 60. The aflatoxins are eluted with chloroform-acetone (90:10) and are detected using bidirectional thin-layer chromatography (TLC) with aluminium silica gel foil. The mean recovery of aflatoxins B1, B2, G1, and G2 in corn samples was 73, 78, 80, and 64%, respectively; the limit of detection was 0.5 micrograms/kg. The results can also be confirmed by derivative formation using trifluoroacetic acid on the TLC plate. The method has been applied to a wide range of foods with good results.
PubMed: 1441760
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pubmed:1441760Le document en format XML
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Zakaria</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1441760</idno><idno type="pmid">1441760</idno><idno type="wicri:Area/PubMed/Corpus">000A34</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000A34</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A rapid, sensitive and economic method for the detection, quantification and confirmation of aflatoxins.</title><author><name sortKey="Majerus, P" sort="Majerus, P" uniqKey="Majerus P" first="P" last="Majerus">P. Majerus</name><affiliation><nlm:affiliation>Chemisches Untersuchungsamt Trier, Federal Republic of Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Zakaria, Z" sort="Zakaria, Z" uniqKey="Zakaria Z" first="Z" last="Zakaria">Z. Zakaria</name></author></analytic><series><title level="j">Zeitschrift fur Lebensmittel-Untersuchung und -Forschung</title><idno type="ISSN">0044-3026</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aflatoxins (analysis)</term><term>Arachis</term><term>Chromatography, Thin Layer</term><term>Cottonseed Oil</term><term>Food Microbiology</term><term>Sensitivity and Specificity</term><term>Zea mays</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Aflatoxins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Arachis</term><term>Chromatography, Thin Layer</term><term>Cottonseed Oil</term><term>Food Microbiology</term><term>Sensitivity and Specificity</term><term>Zea mays</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A rapid, sensitive and economic method for the detection, quantification and confirmation of aflatoxins is described. Aflatoxins B1, B2, G1, and G2, are extracted by methanol/water (85 + 15) and partitioned into methylene dichloride. The methylene dichloride solution is cleaned up on a polypropylene column, filled with 0.5 g silica gel 60. The aflatoxins are eluted with chloroform-acetone (90:10) and are detected using bidirectional thin-layer chromatography (TLC) with aluminium silica gel foil. The mean recovery of aflatoxins B1, B2, G1, and G2 in corn samples was 73, 78, 80, and 64%, respectively; the limit of detection was 0.5 micrograms/kg. The results can also be confirmed by derivative formation using trifluoroacetic acid on the TLC plate. The method has been applied to a wide range of foods with good results.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1441760</PMID><DateCreated><Year>1992</Year><Month>12</Month><Day>16</Day></DateCreated><DateCompleted><Year>1992</Year><Month>12</Month><Day>16</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0044-3026</ISSN><JournalIssue CitedMedium="Print"><Volume>195</Volume><Issue>4</Issue><PubDate><Year>1992</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Zeitschrift fur Lebensmittel-Untersuchung und -Forschung</Title><ISOAbbreviation>Z Lebensm Unters Forsch</ISOAbbreviation></Journal><ArticleTitle>A rapid, sensitive and economic method for the detection, quantification and confirmation of aflatoxins.</ArticleTitle><Pagination><MedlinePgn>316-9</MedlinePgn></Pagination><Abstract><AbstractText>A rapid, sensitive and economic method for the detection, quantification and confirmation of aflatoxins is described. Aflatoxins B1, B2, G1, and G2, are extracted by methanol/water (85 + 15) and partitioned into methylene dichloride. The methylene dichloride solution is cleaned up on a polypropylene column, filled with 0.5 g silica gel 60. The aflatoxins are eluted with chloroform-acetone (90:10) and are detected using bidirectional thin-layer chromatography (TLC) with aluminium silica gel foil. The mean recovery of aflatoxins B1, B2, G1, and G2 in corn samples was 73, 78, 80, and 64%, respectively; the limit of detection was 0.5 micrograms/kg. The results can also be confirmed by derivative formation using trifluoroacetic acid on the TLC plate. The method has been applied to a wide range of foods with good results.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Majerus</LastName><ForeName>P</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Chemisches Untersuchungsamt Trier, Federal Republic of Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zakaria</LastName><ForeName>Z</ForeName><Initials>Z</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Z Lebensm Unters Forsch</MedlineTA><NlmUniqueID>7509812</NlmUniqueID><ISSNLinking>0044-3026</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000348">Aflatoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003369">Cottonseed Oil</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000348" MajorTopicYN="N">Aflatoxins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010367" MajorTopicYN="N">Arachis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002855" MajorTopicYN="N">Chromatography, Thin Layer</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003369" MajorTopicYN="N">Cottonseed Oil</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005516" MajorTopicYN="Y">Food Microbiology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1992</Year><Month>10</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1992</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1992</Year><Month>10</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1441760</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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