Modification of human platelet adhesion on biomaterial surfaces by protein preadsorption under static and flow conditions.
Identifieur interne : 000754 ( PubMed/Corpus ); précédent : 000753; suivant : 000755Modification of human platelet adhesion on biomaterial surfaces by protein preadsorption under static and flow conditions.
Auteurs : Mike Otto ; Arno Franzen ; Torsten Hansen ; Charles James KirkpatrickSource :
- Journal of materials science. Materials in medicine [ 0957-4530 ] ; 2004.
English descriptors
- KwdEn :
- Adsorption, Biocompatible Materials (adverse effects), Biomechanical Phenomena, Blood Proteins (physiology), Blood Vessel Prosthesis (adverse effects), Flow Cytometry, Hemorheology, Humans, In Vitro Techniques, Materials Testing, P-Selectin (physiology), Platelet Activation (physiology), Platelet Adhesiveness (physiology), Thrombosis (etiology).
- MESH :
- chemical , adverse effects : Biocompatible Materials.
- chemical , physiology : Blood Proteins, P-Selectin.
- adverse effects : Blood Vessel Prosthesis.
- etiology : Thrombosis.
- physiology : Platelet Activation, Platelet Adhesiveness.
- Adsorption, Biomechanical Phenomena, Flow Cytometry, Hemorheology, Humans, In Vitro Techniques, Materials Testing.
Abstract
Biomaterial-induced thrombosis remains one of the main complications of vascular implant devices. Preadsorbed proteins on the biomaterial/blood interface will modify the adhesion and activation of platelets (PTLs) during the initial contact-phase. Our results clearly show that PTL-adherence on biomaterials is influenced not only by protein preadsorption, but also by flow conditions. The covalent coating of TCPS and glass by phosphorylcholine (PC) induces a significant decrease of PTL adhesion but leads to a slight, but nevertheless significant activation of PTL, which was detected by the induction of P-selectin expression using FACS analysis. Methodologically, the visualization of PTL adhesion gave more reliable results for measurement of PTL adhesion than the cell-enzyme immunoassay (EIA) for P-selectin. Human citrated plasma caused an inhibition of PTL. It is probable, that the contained sodium citrate may inhibit PTL adhesion by its calcium ion-binding capacity. The flow experiment as dynamic system is in our view absolutely essential for the evaluation of biomaterials for vascular prosthesis, and is in accordance with the international standards. The results of the experiments also suggest that investigations under static and flow conditions are needed to determine the influence of protein adsorption on mixed blood cell populations, for example, on PTL and PMN mixtures/co-cultures in order to achieve a better simulation of the in vivo situation.
PubMed: 15338589
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Mainz, Germany. otto@patho-trier.de</nlm:affiliation></affiliation></author><author><name sortKey="Franzen, Arno" sort="Franzen, Arno" uniqKey="Franzen A" first="Arno" last="Franzen">Arno Franzen</name></author><author><name sortKey="Hansen, Torsten" sort="Hansen, Torsten" uniqKey="Hansen T" first="Torsten" last="Hansen">Torsten Hansen</name></author><author><name sortKey="Kirkpatrick, Charles James" sort="Kirkpatrick, Charles James" uniqKey="Kirkpatrick C" first="Charles James" last="Kirkpatrick">Charles James Kirkpatrick</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15338589</idno><idno type="pmid">15338589</idno><idno type="wicri:Area/PubMed/Corpus">000754</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000754</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Modification of human platelet adhesion on biomaterial surfaces by protein preadsorption under static and flow conditions.</title><author><name sortKey="Otto, Mike" sort="Otto, Mike" uniqKey="Otto M" first="Mike" last="Otto">Mike Otto</name><affiliation><nlm:affiliation>Institute of Pathology, Johannes Gutenberg-University of Mainz, Langenbeckstr. Mainz, Germany. otto@patho-trier.de</nlm:affiliation></affiliation></author><author><name sortKey="Franzen, Arno" sort="Franzen, Arno" uniqKey="Franzen A" first="Arno" last="Franzen">Arno Franzen</name></author><author><name sortKey="Hansen, Torsten" sort="Hansen, Torsten" uniqKey="Hansen T" first="Torsten" last="Hansen">Torsten Hansen</name></author><author><name sortKey="Kirkpatrick, Charles James" sort="Kirkpatrick, Charles James" uniqKey="Kirkpatrick C" first="Charles James" last="Kirkpatrick">Charles James Kirkpatrick</name></author></analytic><series><title level="j">Journal of materials science. 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Preadsorbed proteins on the biomaterial/blood interface will modify the adhesion and activation of platelets (PTLs) during the initial contact-phase. Our results clearly show that PTL-adherence on biomaterials is influenced not only by protein preadsorption, but also by flow conditions. The covalent coating of TCPS and glass by phosphorylcholine (PC) induces a significant decrease of PTL adhesion but leads to a slight, but nevertheless significant activation of PTL, which was detected by the induction of P-selectin expression using FACS analysis. Methodologically, the visualization of PTL adhesion gave more reliable results for measurement of PTL adhesion than the cell-enzyme immunoassay (EIA) for P-selectin. Human citrated plasma caused an inhibition of PTL. It is probable, that the contained sodium citrate may inhibit PTL adhesion by its calcium ion-binding capacity. The flow experiment as dynamic system is in our view absolutely essential for the evaluation of biomaterials for vascular prosthesis, and is in accordance with the international standards. The results of the experiments also suggest that investigations under static and flow conditions are needed to determine the influence of protein adsorption on mixed blood cell populations, for example, on PTL and PMN mixtures/co-cultures in order to achieve a better simulation of the in vivo situation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15338589</PMID><DateCreated><Year>2004</Year><Month>09</Month><Day>01</Day></DateCreated><DateCompleted><Year>2004</Year><Month>10</Month><Day>08</Day></DateCompleted><DateRevised><Year>2014</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0957-4530</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of materials science. Materials in medicine</Title><ISOAbbreviation>J Mater Sci Mater Med</ISOAbbreviation></Journal><ArticleTitle>Modification of human platelet adhesion on biomaterial surfaces by protein preadsorption under static and flow conditions.</ArticleTitle><Pagination><MedlinePgn>35-42</MedlinePgn></Pagination><Abstract><AbstractText>Biomaterial-induced thrombosis remains one of the main complications of vascular implant devices. Preadsorbed proteins on the biomaterial/blood interface will modify the adhesion and activation of platelets (PTLs) during the initial contact-phase. Our results clearly show that PTL-adherence on biomaterials is influenced not only by protein preadsorption, but also by flow conditions. The covalent coating of TCPS and glass by phosphorylcholine (PC) induces a significant decrease of PTL adhesion but leads to a slight, but nevertheless significant activation of PTL, which was detected by the induction of P-selectin expression using FACS analysis. Methodologically, the visualization of PTL adhesion gave more reliable results for measurement of PTL adhesion than the cell-enzyme immunoassay (EIA) for P-selectin. Human citrated plasma caused an inhibition of PTL. It is probable, that the contained sodium citrate may inhibit PTL adhesion by its calcium ion-binding capacity. The flow experiment as dynamic system is in our view absolutely essential for the evaluation of biomaterials for vascular prosthesis, and is in accordance with the international standards. The results of the experiments also suggest that investigations under static and flow conditions are needed to determine the influence of protein adsorption on mixed blood cell populations, for example, on PTL and PMN mixtures/co-cultures in order to achieve a better simulation of the in vivo situation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Otto</LastName><ForeName>Mike</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Pathology, Johannes Gutenberg-University of Mainz, Langenbeckstr. Mainz, Germany. otto@patho-trier.de</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Franzen</LastName><ForeName>Arno</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Hansen</LastName><ForeName>Torsten</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Kirkpatrick</LastName><ForeName>Charles James</ForeName><Initials>CJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Mater Sci Mater Med</MedlineTA><NlmUniqueID>9013087</NlmUniqueID><ISSNLinking>0957-4530</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001672">Biocompatible Materials</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001798">Blood Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019007">P-Selectin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000327" MajorTopicYN="N">Adsorption</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001672" MajorTopicYN="Y">Biocompatible Materials</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="N">adverse effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001696" MajorTopicYN="N">Biomechanical Phenomena</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001798" MajorTopicYN="N">Blood Proteins</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001807" MajorTopicYN="N">Blood Vessel Prosthesis</DescriptorName><QualifierName UI="Q000009" MajorTopicYN="N">adverse effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005434" MajorTopicYN="N">Flow Cytometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018056" MajorTopicYN="N">Hemorheology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D066298" MajorTopicYN="N">In Vitro Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008422" MajorTopicYN="N">Materials Testing</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019007" MajorTopicYN="N">P-Selectin</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015539" MajorTopicYN="N">Platelet Activation</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010973" MajorTopicYN="N">Platelet Adhesiveness</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013927" MajorTopicYN="N">Thrombosis</DescriptorName><QualifierName UI="Q000209" MajorTopicYN="N">etiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>9</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>10</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>9</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15338589</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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