Functional characterization of the 5'-flanking and the promoter region of the human UCP3 (hUCP3) gene.
Identifieur interne : 000046 ( Ncbi/Curation ); précédent : 000045; suivant : 000047Functional characterization of the 5'-flanking and the promoter region of the human UCP3 (hUCP3) gene.
Auteurs : N. Tu [Allemagne] ; H. Chen ; U. Winnikes ; I. Reinert ; K M Pirke ; K U LentesSource :
- Life sciences [ 0024-3205 ] ; 2000.
English descriptors
- KwdEn :
- 5' Untranslated Regions, Animals, Base Sequence, Carrier Proteins (genetics), Cells, Cultured, Chloramphenicol O-Acetyltransferase (genetics), Humans, Ion Channels, Mitochondrial Proteins, Molecular Sequence Data, Organ Specificity, Promoter Regions, Genetic (physiology), RNA, Messenger (analysis), Uncoupling Agents, Uncoupling Protein 3.
- MESH :
- chemical , analysis : RNA, Messenger.
- chemical , genetics : Carrier Proteins, Chloramphenicol O-Acetyltransferase.
- chemical : 5' Untranslated Regions, Ion Channels, Mitochondrial Proteins, Uncoupling Agents, Uncoupling Protein 3.
- physiology : Promoter Regions, Genetic.
- Animals, Base Sequence, Cells, Cultured, Humans, Molecular Sequence Data, Organ Specificity.
Abstract
Uncoupling protein-3 (UCP3) is considered as an important regulator of energy expenditure and thermogenesis in humans. To get insight into the mechanisms regulating its expression we have cloned and characterized about 5 kb of the 5'-flanking region of the human UCP3 (hUCP3) gene. 5'-RACE analysis suggested a single transcription initiation site 187 bp upstream from the translational start site. The promoter region contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific factors like MyoD and MEF2 sites. Functional characterization of a 3 kb hUCP3 promoter fragment in multiple cell lines using a CAT-ELISA identified a cis-acting negative regulatory element between -2983 and -982 while the region between -982 and -284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3.
PubMed: 11045607
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Chen</name></author><author><name sortKey="Winnikes, U" sort="Winnikes, U" uniqKey="Winnikes U" first="U" last="Winnikes">U. Winnikes</name></author><author><name sortKey="Reinert, I" sort="Reinert, I" uniqKey="Reinert I" first="I" last="Reinert">I. Reinert</name></author><author><name sortKey="Pirke, K M" sort="Pirke, K M" uniqKey="Pirke K" first="K M" last="Pirke">K M Pirke</name></author><author><name sortKey="Lentes, K U" sort="Lentes, K U" uniqKey="Lentes K" first="K U" last="Lentes">K U Lentes</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:11045607</idno><idno type="pmid">11045607</idno><idno type="wicri:Area/PubMed/Corpus">000846</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000846</idno><idno type="wicri:Area/PubMed/Curation">000846</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000846</idno><idno type="wicri:Area/PubMed/Checkpoint">000846</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000846</idno><idno type="wicri:Area/Ncbi/Merge">000046</idno><idno type="wicri:Area/Ncbi/Curation">000046</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Functional characterization of the 5'-flanking and the promoter region of the human UCP3 (hUCP3) gene.</title><author><name sortKey="Tu, N" sort="Tu, N" uniqKey="Tu N" first="N" last="Tu">N. Tu</name><affiliation wicri:level="4"><nlm:affiliation>Center for Psychobiological and Psychosomatic Research (FPP), University of Trier, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Psychobiological and Psychosomatic Research (FPP), University of Trier</wicri:regionArea><placeName><settlement type="city">Trèves (Allemagne)</settlement><region type="land" nuts="1">Rhénanie-Palatinat</region></placeName><orgName type="university">Université de Trèves</orgName></affiliation></author><author><name sortKey="Chen, H" sort="Chen, H" uniqKey="Chen H" first="H" last="Chen">H. Chen</name></author><author><name sortKey="Winnikes, U" sort="Winnikes, U" uniqKey="Winnikes U" first="U" last="Winnikes">U. Winnikes</name></author><author><name sortKey="Reinert, I" sort="Reinert, I" uniqKey="Reinert I" first="I" last="Reinert">I. Reinert</name></author><author><name sortKey="Pirke, K M" sort="Pirke, K M" uniqKey="Pirke K" first="K M" last="Pirke">K M Pirke</name></author><author><name sortKey="Lentes, K U" sort="Lentes, K U" uniqKey="Lentes K" first="K U" last="Lentes">K U Lentes</name></author></analytic><series><title level="j">Life sciences</title><idno type="ISSN">0024-3205</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>5' Untranslated Regions</term><term>Animals</term><term>Base Sequence</term><term>Carrier Proteins (genetics)</term><term>Cells, Cultured</term><term>Chloramphenicol O-Acetyltransferase (genetics)</term><term>Humans</term><term>Ion Channels</term><term>Mitochondrial Proteins</term><term>Molecular Sequence Data</term><term>Organ Specificity</term><term>Promoter Regions, Genetic (physiology)</term><term>RNA, Messenger (analysis)</term><term>Uncoupling Agents</term><term>Uncoupling Protein 3</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carrier Proteins</term><term>Chloramphenicol O-Acetyltransferase</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>5' Untranslated Regions</term><term>Ion Channels</term><term>Mitochondrial Proteins</term><term>Uncoupling Agents</term><term>Uncoupling Protein 3</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Promoter Regions, Genetic</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cells, Cultured</term><term>Humans</term><term>Molecular Sequence Data</term><term>Organ Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Uncoupling protein-3 (UCP3) is considered as an important regulator of energy expenditure and thermogenesis in humans. To get insight into the mechanisms regulating its expression we have cloned and characterized about 5 kb of the 5'-flanking region of the human UCP3 (hUCP3) gene. 5'-RACE analysis suggested a single transcription initiation site 187 bp upstream from the translational start site. The promoter region contains both TATA and CAAT boxes as well as consensus motifs for PPRE, TRE, CRE and muscle-specific factors like MyoD and MEF2 sites. Functional characterization of a 3 kb hUCP3 promoter fragment in multiple cell lines using a CAT-ELISA identified a cis-acting negative regulatory element between -2983 and -982 while the region between -982 and -284 showed greatly increased basal promoter activity suggesting the presence of a strong enhancer element. Promoter activity was particularly enhanced in the murine skeletal muscle cell line C2C12 reflecting the tissue-selective expression pattern of UCP3.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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