Proteomic analysis of the cortisol‐mediated stress response in THP‐1 monocytes using DIGE technology
Identifieur interne : 001322 ( Main/Exploration ); précédent : 001321; suivant : 001323Proteomic analysis of the cortisol‐mediated stress response in THP‐1 monocytes using DIGE technology
Auteurs : Anja M. Billing [Luxembourg (pays), Allemagne] ; Fred Fack [Luxembourg (pays)] ; Jenny Renaut [Luxembourg (pays)] ; Christophe M. Olinger [Luxembourg (pays)] ; Andrea B. Schote [Luxembourg (pays), Allemagne] ; Jonathan D. Turner [Luxembourg (pays), Allemagne] ; Claude P. Muller [Luxembourg (pays), Allemagne]Source :
- Journal of Mass Spectrometry [ 1076-5174 ] ; 2007-11.
Descripteurs français
- Pascal (Inist)
- Antiinflammatoire, Apoptose, Cellule tumorale, Cytotoxicité, Electrophorèse bidimensionnelle, Electrophorèse gel, Elément réponse, Expression génique, FKBP51, Glucocorticoïde, Génomique, Homme, Hydrocortisone, Immunité naturelle, In vitro, Leucocyte, Leucémie, Ligand, Lignée THP1, Lignée cellulaire, Monocyte, Mort cellulaire, Mécanisme action, Méthode analyse, Promoteur transcription, Protéine, Protéomique, Récepteur glucocorticoïde, Récepteur hormonal, Spectrométrie masse, Stress, Stéroïde, Système immunitaire.
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
- 2D DIGE, Analysis method, Antiinflammatory agent, Apoptosis, Cell death, Cell line, Cytotoxicity, FKBP51, Gel electrophoresis, Gene expression, Genomics, Glucocorticoid, Glucocorticoid receptor, Hormonal receptor, Human, Hydrocortisone, Immune system, In vitro, Leukemia, Leukocyte, Ligand, Mass spectrometry, Mechanism of action, Monocyte, Natural immunity, Protein, Proteomics, Response element, Steroid, Stress, Transcription promoter, Tumor cell, Two dimensional electrophoresis, alternative promoters, cortisol, glucocorticoid receptor, glucocorticoid response element.
Abstract
The glucocorticoid (GC) cortisol, the main mediator of the hypothalamic‐pituitary‐adrenal axis has many implications in metabolism, stress response and the immune system. Its function is mediated via binding to the glucocorticoid receptor (GR), a member of the superfamily of ligand‐activated nuclear hormone receptors. The activity of the ligated GR results from its binding as a transcription factor to glucocorticoid response elements (GREs). Two‐dimensional gel electrophoresis with DIGE (fluorescence difference gel electrophoresis) technology was applied to study the effects of cortisol on the human THP‐1 monocytic cell line. A total of 28 cortisol‐modulated proteins were identified belonging to five functional groups: cytoskeleton (8), chaperones (9), immune response (4), metabolism (3) and transcription/translation (4). Their corresponding genes were screened for putative GREs in their + 10 kb/− 0.2 kb promoter regions including all alternative promoters available within the Database for Transcription Start Sites (DBTSS). FKBP51, known to be induced by cortisol, was identified as the strongest differentially expressed protein, and contains the highest number of strict GREs. Genomic analysis of five alternative FKBP5 promoter regions suggests GC inducibility of all transcripts. Additionally, proteomics (2D DIGE and 2D immunoblotting) revealed the existence of several FKBP51 isoforms, which were not previously described. To our knowledge this is the first proteomic study that addresses the effects of cortisol on immune cells. FKBP51 isoforms found on the gel map were linked to alternative promoter usage on the genetic level, successfully correlating both the specific proteomic and genomic findings. Copyright © 2007 John Wiley & Sons, Ltd.
Url:
DOI: 10.1002/jms.1270
Affiliations:
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Le document en format XML
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Muller</name><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Luxembourg (pays)</country><wicri:regionArea>Institute of Immunology, National Public Health Laboratory, 20A, rue Auguste Lumière, L‐1950 Luxembourg</wicri:regionArea><wicri:noRegion>L‐1950 Luxembourg</wicri:noRegion></affiliation><affiliation wicri:level="4"><country xml:lang="fr">Allemagne</country><wicri:regionArea>Graduate School of Psychobiology, University of Trier, Johanniterufer 15, D‐54290 Trier</wicri:regionArea><orgName type="university">Université de Trèves</orgName><placeName><settlement type="city">Trèves (Allemagne)</settlement><region type="land" nuts="1">Rhénanie-Palatinat</region></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr" wicri:curation="lc">Luxembourg (pays)</country><wicri:regionArea>20A, rue Auguste Lumière, L‐1950 Luxembourg</wicri:regionArea><wicri:noRegion>L‐1950 Luxembourg</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Mass Spectrometry</title><title level="j" type="abbrev">J. Mass Spectrom.</title><idno type="ISSN">1076-5174</idno><idno type="eISSN">1096-9888</idno><imprint><publisher>John Wiley & Sons, Ltd.</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2007-11">2007-11</date><biblScope unit="volume">42</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="1433">1433</biblScope><biblScope unit="page" to="1444">1444</biblScope></imprint><idno type="ISSN">1076-5174</idno></series><idno type="istex">F07F97219D30654A52E9FDE4822183C262C2B77C</idno><idno type="DOI">10.1002/jms.1270</idno><idno type="ArticleID">JMS1270</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1076-5174</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>2D DIGE</term><term>Analysis method</term><term>Antiinflammatory agent</term><term>Apoptosis</term><term>Cell death</term><term>Cell line</term><term>Cytotoxicity</term><term>FKBP51</term><term>Gel electrophoresis</term><term>Gene expression</term><term>Genomics</term><term>Glucocorticoid</term><term>Glucocorticoid receptor</term><term>Hormonal receptor</term><term>Human</term><term>Hydrocortisone</term><term>Immune system</term><term>In vitro</term><term>Leukemia</term><term>Leukocyte</term><term>Ligand</term><term>Mass spectrometry</term><term>Mechanism of action</term><term>Monocyte</term><term>Natural immunity</term><term>Protein</term><term>Proteomics</term><term>Response element</term><term>Steroid</term><term>Stress</term><term>Transcription promoter</term><term>Tumor cell</term><term>Two dimensional electrophoresis</term><term>alternative promoters</term><term>cortisol</term><term>glucocorticoid receptor</term><term>glucocorticoid response element</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Antiinflammatoire</term><term>Apoptose</term><term>Cellule tumorale</term><term>Cytotoxicité</term><term>Electrophorèse bidimensionnelle</term><term>Electrophorèse gel</term><term>Elément réponse</term><term>Expression génique</term><term>FKBP51</term><term>Glucocorticoïde</term><term>Génomique</term><term>Homme</term><term>Hydrocortisone</term><term>Immunité naturelle</term><term>In vitro</term><term>Leucocyte</term><term>Leucémie</term><term>Ligand</term><term>Lignée THP1</term><term>Lignée cellulaire</term><term>Monocyte</term><term>Mort cellulaire</term><term>Mécanisme action</term><term>Méthode analyse</term><term>Promoteur transcription</term><term>Protéine</term><term>Protéomique</term><term>Récepteur glucocorticoïde</term><term>Récepteur hormonal</term><term>Spectrométrie masse</term><term>Stress</term><term>Stéroïde</term><term>Système immunitaire</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The glucocorticoid (GC) cortisol, the main mediator of the hypothalamic‐pituitary‐adrenal axis has many implications in metabolism, stress response and the immune system. Its function is mediated via binding to the glucocorticoid receptor (GR), a member of the superfamily of ligand‐activated nuclear hormone receptors. The activity of the ligated GR results from its binding as a transcription factor to glucocorticoid response elements (GREs). Two‐dimensional gel electrophoresis with DIGE (fluorescence difference gel electrophoresis) technology was applied to study the effects of cortisol on the human THP‐1 monocytic cell line. A total of 28 cortisol‐modulated proteins were identified belonging to five functional groups: cytoskeleton (8), chaperones (9), immune response (4), metabolism (3) and transcription/translation (4). Their corresponding genes were screened for putative GREs in their + 10 kb/− 0.2 kb promoter regions including all alternative promoters available within the Database for Transcription Start Sites (DBTSS). FKBP51, known to be induced by cortisol, was identified as the strongest differentially expressed protein, and contains the highest number of strict GREs. Genomic analysis of five alternative FKBP5 promoter regions suggests GC inducibility of all transcripts. Additionally, proteomics (2D DIGE and 2D immunoblotting) revealed the existence of several FKBP51 isoforms, which were not previously described. To our knowledge this is the first proteomic study that addresses the effects of cortisol on immune cells. FKBP51 isoforms found on the gel map were linked to alternative promoter usage on the genetic level, successfully correlating both the specific proteomic and genomic findings. Copyright © 2007 John Wiley & Sons, Ltd.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Luxembourg (pays)</li></country><region><li>Rhénanie-Palatinat</li></region><settlement><li>Trèves (Allemagne)</li></settlement><orgName><li>Université de Trèves</li></orgName></list><tree><country name="Luxembourg (pays)"><noRegion><name sortKey="Billing, Anja M" sort="Billing, Anja M" uniqKey="Billing A" first="Anja M." last="Billing">Anja M. Billing</name></noRegion><name sortKey="Fack, Fred" sort="Fack, Fred" uniqKey="Fack F" first="Fred" last="Fack">Fred Fack</name><name sortKey="Muller, Claude P" sort="Muller, Claude P" uniqKey="Muller C" first="Claude P." last="Muller">Claude P. Muller</name><name sortKey="Muller, Claude P" sort="Muller, Claude P" uniqKey="Muller C" first="Claude P." last="Muller">Claude P. Muller</name><name sortKey="Olinger, Christophe M" sort="Olinger, Christophe M" uniqKey="Olinger C" first="Christophe M." last="Olinger">Christophe M. Olinger</name><name sortKey="Renaut, Jenny" sort="Renaut, Jenny" uniqKey="Renaut J" first="Jenny" last="Renaut">Jenny Renaut</name><name sortKey="Schote, Andrea B" sort="Schote, Andrea B" uniqKey="Schote A" first="Andrea B." last="Schote">Andrea B. Schote</name><name sortKey="Turner, Jonathan D" sort="Turner, Jonathan D" uniqKey="Turner J" first="Jonathan D." last="Turner">Jonathan D. Turner</name></country><country name="Allemagne"><region name="Rhénanie-Palatinat"><name sortKey="Billing, Anja M" sort="Billing, Anja M" uniqKey="Billing A" first="Anja M." last="Billing">Anja M. Billing</name></region><name sortKey="Muller, Claude P" sort="Muller, Claude P" uniqKey="Muller C" first="Claude P." last="Muller">Claude P. Muller</name><name sortKey="Schote, Andrea B" sort="Schote, Andrea B" uniqKey="Schote A" first="Andrea B." last="Schote">Andrea B. Schote</name><name sortKey="Turner, Jonathan D" sort="Turner, Jonathan D" uniqKey="Turner J" first="Jonathan D." last="Turner">Jonathan D. 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