The heads of the measles virus attachment protein move to transmit the fusion-triggering signal
Identifieur interne : 000936 ( Main/Exploration ); précédent : 000935; suivant : 000937The heads of the measles virus attachment protein move to transmit the fusion-triggering signal
Auteurs : Chanakha K. Navaratnarajah [États-Unis] ; Numan Oezguen [États-Unis] ; Levi Rupp [États-Unis] ; Leah Kay [États-Unis] ; Vincent H J. Leonard [États-Unis] ; Werner Braun [États-Unis] ; Roberto Cattaneo [États-Unis]Source :
- Nature Structural & Molecular Biology [ 1545-9993 ] ; 2011-02.
Abstract
The measles virus entry system, consisting of attachment (hemagglutinin, H) and fusion proteins, operates by means of a variety of natural and targeted receptors; however, the mechanism that triggers fusion of the viral envelope with the plasma membrane is not understood. Here, we tested a model proposing that the two heads of an H dimer, which are covalently linked at their base, after binding two receptor molecules, move relative to each other to transmit the fusion-triggering signal. Indeed, stabilizing the H-dimer interface with additional intermolecular disulfide bonds prevented membrane fusion, an effect that was reversed by a reducing agent. Moreover, a membrane-anchored designated receptor efficiently triggered fusion, provided that it engaged the H dimer at locations proximal to where the natural receptors bind and distal to the H-dimer interface. We discuss how receptors may force H-protein heads to switch partners and transmit the fusion-triggering signal.
Url:
DOI: 10.1038/nsmb.1967
Affiliations:
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All Rights Reserved.</publisher><date type="published" when="2011-02">2011-02</date><biblScope unit="volume">18</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="128">128</biblScope><biblScope unit="page" to="134">134</biblScope></imprint><idno type="ISSN">1545-9993</idno></series><idno type="istex">B8D1DB0332D5C4763449D8B5A6A2D3821B0C9BEC</idno><idno type="DOI">10.1038/nsmb.1967</idno><idno type="ArticleID">nsmb.1967</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1545-9993</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="eng">The measles virus entry system, consisting of attachment (hemagglutinin, H) and fusion proteins, operates by means of a variety of natural and targeted receptors; however, the mechanism that triggers fusion of the viral envelope with the plasma membrane is not understood. Here, we tested a model proposing that the two heads of an H dimer, which are covalently linked at their base, after binding two receptor molecules, move relative to each other to transmit the fusion-triggering signal. Indeed, stabilizing the H-dimer interface with additional intermolecular disulfide bonds prevented membrane fusion, an effect that was reversed by a reducing agent. Moreover, a membrane-anchored designated receptor efficiently triggered fusion, provided that it engaged the H dimer at locations proximal to where the natural receptors bind and distal to the H-dimer interface. We discuss how receptors may force H-protein heads to switch partners and transmit the fusion-triggering signal.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Minnesota</li><li>Texas</li></region></list><tree><country name="États-Unis"><region name="Minnesota"><name sortKey="Navaratnarajah, Chanakha K" sort="Navaratnarajah, Chanakha K" uniqKey="Navaratnarajah C" first="Chanakha K" last="Navaratnarajah">Chanakha K. Navaratnarajah</name></region><name sortKey="Braun, Werner" sort="Braun, Werner" uniqKey="Braun W" first="Werner" last="Braun">Werner Braun</name><name sortKey="Braun, Werner" sort="Braun, Werner" uniqKey="Braun W" first="Werner" last="Braun">Werner Braun</name><name sortKey="Cattaneo, Roberto" sort="Cattaneo, Roberto" uniqKey="Cattaneo R" first="Roberto" last="Cattaneo">Roberto Cattaneo</name><name sortKey="Cattaneo, Roberto" sort="Cattaneo, Roberto" uniqKey="Cattaneo R" first="Roberto" last="Cattaneo">Roberto Cattaneo</name><name sortKey="Cattaneo, Roberto" sort="Cattaneo, Roberto" uniqKey="Cattaneo R" first="Roberto" last="Cattaneo">Roberto Cattaneo</name><name sortKey="Kay, Leah" sort="Kay, Leah" uniqKey="Kay L" first="Leah" last="Kay">Leah Kay</name><name sortKey="Kay, Leah" sort="Kay, Leah" uniqKey="Kay L" first="Leah" last="Kay">Leah Kay</name><name sortKey="Leonard, Vincent H J" sort="Leonard, Vincent H J" uniqKey="Leonard V" first="Vincent H J" last="Leonard">Vincent H J. Leonard</name><name sortKey="Leonard, Vincent H J" sort="Leonard, Vincent H J" uniqKey="Leonard V" first="Vincent H J" last="Leonard">Vincent H J. Leonard</name><name sortKey="Navaratnarajah, Chanakha K" sort="Navaratnarajah, Chanakha K" uniqKey="Navaratnarajah C" first="Chanakha K" last="Navaratnarajah">Chanakha K. Navaratnarajah</name><name sortKey="Oezguen, Numan" sort="Oezguen, Numan" uniqKey="Oezguen N" first="Numan" last="Oezguen">Numan Oezguen</name><name sortKey="Oezguen, Numan" sort="Oezguen, Numan" uniqKey="Oezguen N" first="Numan" last="Oezguen">Numan Oezguen</name><name sortKey="Rupp, Levi" sort="Rupp, Levi" uniqKey="Rupp L" first="Levi" last="Rupp">Levi Rupp</name><name sortKey="Rupp, Levi" sort="Rupp, Levi" uniqKey="Rupp L" first="Levi" last="Rupp">Levi Rupp</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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