Preanalytical variables and performance of diagnostic RNA-based gene expression analysis in breast cancer
Identifieur interne : 000345 ( Main/Exploration ); précédent : 000344; suivant : 000346Preanalytical variables and performance of diagnostic RNA-based gene expression analysis in breast cancer
Auteurs : Christopher Poremba [Allemagne] ; Jennifer Uhlendorff [Allemagne] ; Berit M. Pfitzner [Allemagne] ; Guido Hennig [Allemagne] ; Kerstin Bohmann [Allemagne] ; Hans Bojar [Allemagne] ; Veit Krenn [Allemagne] ; Jan C. Brase [Allemagne] ; Franziska Haufe [Allemagne] ; Manuela Averdick [Allemagne] ; Manfred Dietel [Allemagne] ; Ralf Kronenwett [Allemagne] ; Carsten Denkert [Allemagne]Source :
- Virchows Archiv [ 0945-6317 ] ; 2014.
Abstract
Prognostic multigene expression assays have become widely available to provide additional information to standard clinical parameters and to support clinicians in treatment decisions. In this study, we analyzed the impact of variations in tissue handling on the diagnostic EndoPredict test results. EndoPredict is a quantitative reverse transcription PCR assay conducted on RNA from formalin-fixed, paraffin-embedded (FFPE) tissue that predicts the likelihood of distant recurrence in patients with ER-positive/HER2-negative breast cancer. In this study, we performed a total of 138 EndoPredict assays to study the effects of preanalytical variables such as time to fixation, fixation time, tumor cell content, and section storage time on the EndoPredict test results. A time to fixation of up to 12 h and fixation of up to 5 days did not affect the results of the gene expression test. Paired samples of FFPE sections with tumor cell content ranging from 15 to 95 % and tumor-enriched samples showed a correlation coefficient of 0.97. Test results of tissue sections that have been stored for 12 months at +4 or +20 °C showed a correlation of 0.99 when compared to results of nonstored sections. In conclusion, preanalytical tissue handling is not a critical factor for diagnostic gene expression analysis with the EndoPredict assay. The test can therefore be easily integrated into the standard workflow of molecular pathology.
Url:
DOI: 10.1007/s00428-014-1652-0
PubMed: 25218890
PubMed Central: 4180906
Affiliations:
- Allemagne
- Bavière, Berlin, District de Cologne, District de Düsseldorf, District de Haute-Bavière, Rhénanie-du-Nord-Westphalie
- Berlin, Cologne, Düsseldorf, Munich
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Pfitzner</name><affiliation wicri:level="3"><nlm:aff id="Aff4">Institute of Pathology, Charité Hospital, Campus Mitte, Charitéplatz 1, 10117 Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, Charité Hospital, Campus Mitte, Charitéplatz 1, 10117 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Hennig, Guido" sort="Hennig, Guido" uniqKey="Hennig G" first="Guido" last="Hennig">Guido Hennig</name><affiliation wicri:level="1"><nlm:aff id="Aff5">Siemens Healthcare Diagnostics Holding GmbH, Ludwig-Erhard-Straße 12, 65760 Eschborn, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Siemens Healthcare Diagnostics Holding GmbH, Ludwig-Erhard-Straße 12, 65760 Eschborn</wicri:regionArea><wicri:noRegion>65760 Eschborn</wicri:noRegion><wicri:noRegion>65760 Eschborn</wicri:noRegion><wicri:noRegion>65760 Eschborn</wicri:noRegion></affiliation></author><author><name sortKey="Bohmann, Kerstin" sort="Bohmann, Kerstin" uniqKey="Bohmann K" first="Kerstin" last="Bohmann">Kerstin Bohmann</name><affiliation wicri:level="3"><nlm:aff id="Aff3">Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Bojar, Hans" sort="Bojar, Hans" uniqKey="Bojar H" first="Hans" last="Bojar">Hans Bojar</name><affiliation wicri:level="3"><nlm:aff id="Aff2">Center for Molecular Oncology and Molecular Pathology (ZMOMP), Hans-Günther-Sohl-Straße 12, 40235 Düsseldorf, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Center for Molecular Oncology and Molecular Pathology (ZMOMP), Hans-Günther-Sohl-Straße 12, 40235 Düsseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Krenn, Veit" sort="Krenn, Veit" uniqKey="Krenn V" first="Veit" last="Krenn">Veit Krenn</name><affiliation wicri:level="1"><nlm:aff id="Aff6">Medical Care Center for Histology, Cytology and Molecular Diagnostics, Max-Planck-Str. 5, 54296 Trier, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Medical Care Center for Histology, Cytology and Molecular Diagnostics, Max-Planck-Str. 5, 54296 Trier</wicri:regionArea><wicri:noRegion>54296 Trier</wicri:noRegion><wicri:noRegion>54296 Trier</wicri:noRegion><wicri:noRegion>54296 Trier</wicri:noRegion></affiliation></author><author><name sortKey="Brase, Jan C" sort="Brase, Jan C" uniqKey="Brase J" first="Jan C." last="Brase">Jan C. Brase</name><affiliation wicri:level="3"><nlm:aff id="Aff3">Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Haufe, Franziska" sort="Haufe, Franziska" uniqKey="Haufe F" first="Franziska" last="Haufe">Franziska Haufe</name><affiliation wicri:level="3"><nlm:aff id="Aff3">Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Averdick, Manuela" sort="Averdick, Manuela" uniqKey="Averdick M" first="Manuela" last="Averdick">Manuela Averdick</name><affiliation wicri:level="3"><nlm:aff id="Aff3">Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation></author><author><name sortKey="Dietel, Manfred" sort="Dietel, Manfred" uniqKey="Dietel M" first="Manfred" last="Dietel">Manfred Dietel</name><affiliation wicri:level="3"><nlm:aff id="Aff4">Institute of Pathology, Charité Hospital, Campus Mitte, Charitéplatz 1, 10117 Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, Charité Hospital, Campus Mitte, Charitéplatz 1, 10117 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author><author><name sortKey="Kronenwett, Ralf" sort="Kronenwett, Ralf" uniqKey="Kronenwett R" first="Ralf" last="Kronenwett">Ralf Kronenwett</name><affiliation wicri:level="3"><nlm:aff id="Aff3">Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Sividon Diagnostics GmbH, Nattermannallee 1, 50829 Cologne</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Cologne</region><settlement type="city">Cologne</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="Aff7">Heinrich-Heine-University Düsseldorf, Medical Faculty, Universitätsstraße 1, 40225 Düsseldorf, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Heinrich-Heine-University Düsseldorf, Medical Faculty, Universitätsstraße 1, 40225 Düsseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Denkert, Carsten" sort="Denkert, Carsten" uniqKey="Denkert C" first="Carsten" last="Denkert">Carsten Denkert</name><affiliation wicri:level="3"><nlm:aff id="Aff4">Institute of Pathology, Charité Hospital, Campus Mitte, Charitéplatz 1, 10117 Berlin, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>Institute of Pathology, Charité Hospital, Campus Mitte, Charitéplatz 1, 10117 Berlin</wicri:regionArea><placeName><region type="land" nuts="3">Berlin</region><settlement type="city">Berlin</settlement></placeName></affiliation></author></analytic><series><title level="j">Virchows Archiv</title><idno type="ISSN">0945-6317</idno><idno type="eISSN">1432-2307</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Prognostic multigene expression assays have become widely available to provide additional information to standard clinical parameters and to support clinicians in treatment decisions. In this study, we analyzed the impact of variations in tissue handling on the diagnostic EndoPredict test results. EndoPredict is a quantitative reverse transcription PCR assay conducted on RNA from formalin-fixed, paraffin-embedded (FFPE) tissue that predicts the likelihood of distant recurrence in patients with ER-positive/HER2-negative breast cancer. In this study, we performed a total of 138 EndoPredict assays to study the effects of preanalytical variables such as time to fixation, fixation time, tumor cell content, and section storage time on the EndoPredict test results. A time to fixation of up to 12 h and fixation of up to 5 days did not affect the results of the gene expression test. Paired samples of FFPE sections with tumor cell content ranging from 15 to 95 % and tumor-enriched samples showed a correlation coefficient of 0.97. Test results of tissue sections that have been stored for 12 months at +4 or +20 °C showed a correlation of 0.99 when compared to results of nonstored sections. In conclusion, preanalytical tissue handling is not a critical factor for diagnostic gene expression analysis with the EndoPredict assay. The test can therefore be easily integrated into the standard workflow of molecular pathology.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Filipits, M" uniqKey="Filipits M">M Filipits</name></author><author><name sortKey="Rudas, M" uniqKey="Rudas M">M Rudas</name></author><author><name sortKey="Jakesz, R" uniqKey="Jakesz R">R Jakesz</name></author><author><name sortKey="Dubsky, P" uniqKey="Dubsky P">P Dubsky</name></author><author><name sortKey="Fitzal, F" uniqKey="Fitzal F">F Fitzal</name></author><author><name sortKey="Singer, Cf" uniqKey="Singer C">CF Singer</name></author><author><name sortKey="Dietze, O" uniqKey="Dietze O">O Dietze</name></author><author><name sortKey="Greil, R" uniqKey="Greil R">R Greil</name></author><author><name sortKey="Jelen, A" uniqKey="Jelen A">A Jelen</name></author><author><name sortKey="Sevelda, P" uniqKey="Sevelda P">P Sevelda</name></author><author><name sortKey="Freibauer, C" uniqKey="Freibauer C">C Freibauer</name></author><author><name sortKey="Muller, V" uniqKey="Muller V">V Muller</name></author><author><name sortKey="Janicke, F" uniqKey="Janicke F">F Janicke</name></author><author><name sortKey="Schmidt, M" uniqKey="Schmidt M">M Schmidt</name></author><author><name sortKey="Kolbl, H" uniqKey="Kolbl H">H Kolbl</name></author><author><name sortKey="Rody, A" uniqKey="Rody A">A Rody</name></author><author><name sortKey="Kaufmann, M" uniqKey="Kaufmann M">M Kaufmann</name></author><author><name sortKey="Schroth, W" uniqKey="Schroth W">W Schroth</name></author><author><name sortKey="Brauch, H" uniqKey="Brauch H">H Brauch</name></author><author><name sortKey="Schwab, M" uniqKey="Schwab M">M Schwab</name></author><author><name sortKey="Fritz, P" uniqKey="Fritz P">P Fritz</name></author><author><name sortKey="Weber, Ke" uniqKey="Weber K">KE Weber</name></author><author><name sortKey="Feder, Is" uniqKey="Feder I">IS Feder</name></author><author><name sortKey="Hennig, G" uniqKey="Hennig G">G Hennig</name></author><author><name sortKey="Kronenwett, R" uniqKey="Kronenwett R">R Kronenwett</name></author><author><name sortKey="Gehrmann, M" uniqKey="Gehrmann M">M Gehrmann</name></author><author><name sortKey="Gnant, M" uniqKey="Gnant M">M Gnant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Paik, S" uniqKey="Paik S">S Paik</name></author><author><name sortKey="Shak, S" uniqKey="Shak S">S Shak</name></author><author><name sortKey="Tang, G" uniqKey="Tang G">G Tang</name></author><author><name sortKey="Kim, C" uniqKey="Kim C">C Kim</name></author><author><name sortKey="Baker, J" uniqKey="Baker J">J Baker</name></author><author><name sortKey="Cronin, M" uniqKey="Cronin M">M Cronin</name></author><author><name sortKey="Baehner, Fl" uniqKey="Baehner F">FL Baehner</name></author><author><name sortKey="Walker, Mg" uniqKey="Walker M">MG Walker</name></author><author><name sortKey="Watson, D" uniqKey="Watson D">D Watson</name></author><author><name sortKey="Park, T" uniqKey="Park T">T Park</name></author><author><name sortKey="Hiller, W" uniqKey="Hiller W">W Hiller</name></author><author><name sortKey="Fisher, Er" uniqKey="Fisher E">ER Fisher</name></author><author><name sortKey="Wickerham, Dl" uniqKey="Wickerham D">DL Wickerham</name></author><author><name sortKey="Bryant, J" uniqKey="Bryant J">J Bryant</name></author><author><name sortKey="Wolmark, N" uniqKey="Wolmark N">N Wolmark</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parker, Js" uniqKey="Parker J">JS Parker</name></author><author><name sortKey="Mullins, M" uniqKey="Mullins M">M Mullins</name></author><author><name sortKey="Cheang, Mc" uniqKey="Cheang M">MC Cheang</name></author><author><name sortKey="Leung, S" uniqKey="Leung S">S Leung</name></author><author><name sortKey="Voduc, D" uniqKey="Voduc D">D Voduc</name></author><author><name sortKey="Vickery, T" uniqKey="Vickery T">T Vickery</name></author><author><name sortKey="Davies, S" uniqKey="Davies S">S Davies</name></author><author><name sortKey="Fauron, C" uniqKey="Fauron C">C Fauron</name></author><author><name sortKey="He, X" uniqKey="He X">X He</name></author><author><name sortKey="Hu, Z" uniqKey="Hu Z">Z Hu</name></author><author><name sortKey="Quackenbush, Jf" uniqKey="Quackenbush J">JF Quackenbush</name></author><author><name sortKey="Stijleman, Ij" uniqKey="Stijleman I">IJ Stijleman</name></author><author><name sortKey="Palazzo, J" uniqKey="Palazzo J">J Palazzo</name></author><author><name sortKey="Marron, Js" uniqKey="Marron J">JS Marron</name></author><author><name sortKey="Nobel, Ab" uniqKey="Nobel A">AB Nobel</name></author><author><name sortKey="Mardis, E" uniqKey="Mardis E">E Mardis</name></author><author><name sortKey="Nielsen, To" uniqKey="Nielsen T">TO Nielsen</name></author><author><name sortKey="Ellis, Mj" uniqKey="Ellis M">MJ Ellis</name></author><author><name sortKey="Perou, Cm" uniqKey="Perou C">CM Perou</name></author><author><name sortKey="Bernard, Ps" uniqKey="Bernard P">PS Bernard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Veer, Lj" uniqKey="Van Veer L">LJ van’t Veer</name></author><author><name sortKey="Dai, H" uniqKey="Dai H">H Dai</name></author><author><name sortKey="Van De Vijver, Mj" uniqKey="Van De Vijver M">MJ van de Vijver</name></author><author><name sortKey="He, Yd" uniqKey="He Y">YD He</name></author><author><name sortKey="Hart, Aa" uniqKey="Hart A">AA Hart</name></author><author><name sortKey="Mao, M" uniqKey="Mao M">M Mao</name></author><author><name sortKey="Peterse, Hl" uniqKey="Peterse H">HL Peterse</name></author><author><name sortKey="Van Der Kooy, K" uniqKey="Van Der Kooy K">K van der Kooy</name></author><author><name sortKey="Marton, Mj" uniqKey="Marton M">MJ Marton</name></author><author><name sortKey="Witteveen, At" uniqKey="Witteveen A">AT Witteveen</name></author><author><name sortKey="Schreiber, Gj" uniqKey="Schreiber G">GJ Schreiber</name></author><author><name sortKey="Kerkhoven, Rm" uniqKey="Kerkhoven R">RM Kerkhoven</name></author><author><name sortKey="Roberts, C" uniqKey="Roberts C">C Roberts</name></author><author><name sortKey="Linsley, Ps" uniqKey="Linsley P">PS Linsley</name></author><author><name sortKey="Bernards, R" uniqKey="Bernards R">R Bernards</name></author><author><name sortKey="Friend, Sh" uniqKey="Friend S">SH Friend</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Specht, K" uniqKey="Specht K">K Specht</name></author><author><name sortKey="Richter, T" uniqKey="Richter T">T Richter</name></author><author><name sortKey="Muller, U" uniqKey="Muller U">U Muller</name></author><author><name sortKey="Walch, A" uniqKey="Walch A">A Walch</name></author><author><name sortKey="Werner, M" uniqKey="Werner M">M Werner</name></author><author><name sortKey="Hofler, H" uniqKey="Hofler H">H Hofler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stanta, G" uniqKey="Stanta G">G Stanta</name></author><author><name sortKey="Bonin, S" uniqKey="Bonin S">S Bonin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hatzis, C" uniqKey="Hatzis C">C Hatzis</name></author><author><name sortKey="Sun, H" uniqKey="Sun H">H Sun</name></author><author><name sortKey="Yao, H" uniqKey="Yao H">H Yao</name></author><author><name sortKey="Hubbard, Re" uniqKey="Hubbard R">RE Hubbard</name></author><author><name sortKey="Meric Bernstam, F" uniqKey="Meric Bernstam F">F Meric-Bernstam</name></author><author><name sortKey="Babiera, Gv" uniqKey="Babiera G">GV Babiera</name></author><author><name sortKey="Wu, Y" uniqKey="Wu Y">Y Wu</name></author><author><name sortKey="Pusztai, L" uniqKey="Pusztai L">L Pusztai</name></author><author><name sortKey="Symmans, Wf" uniqKey="Symmans W">WF Symmans</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hewitt, Sm" uniqKey="Hewitt S">SM Hewitt</name></author><author><name sortKey="Lewis, Fa" uniqKey="Lewis F">FA Lewis</name></author><author><name sortKey="Cao, Y" uniqKey="Cao Y">Y Cao</name></author><author><name sortKey="Conrad, Rc" uniqKey="Conrad R">RC Conrad</name></author><author><name sortKey="Cronin, M" uniqKey="Cronin M">M Cronin</name></author><author><name sortKey="Danenberg, Kd" uniqKey="Danenberg K">KD Danenberg</name></author><author><name sortKey="Goralski, Tj" uniqKey="Goralski T">TJ Goralski</name></author><author><name sortKey="Langmore, Jp" uniqKey="Langmore J">JP Langmore</name></author><author><name sortKey="Raja, Rg" uniqKey="Raja R">RG Raja</name></author><author><name sortKey="Williams, Pm" uniqKey="Williams P">PM Williams</name></author><author><name sortKey="Palma, Jf" uniqKey="Palma J">JF Palma</name></author><author><name sortKey="Warrington, Ja" uniqKey="Warrington J">JA Warrington</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Elloumi, F" uniqKey="Elloumi F">F Elloumi</name></author><author><name sortKey="Hu, Z" uniqKey="Hu Z">Z Hu</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name></author><author><name sortKey="Parker, Js" uniqKey="Parker J">JS Parker</name></author><author><name sortKey="Gulley, Ml" uniqKey="Gulley M">ML Gulley</name></author><author><name sortKey="Amos, Kd" uniqKey="Amos K">KD Amos</name></author><author><name sortKey="Troester, Ma" uniqKey="Troester M">MA Troester</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kotoula, V" uniqKey="Kotoula V">V Kotoula</name></author><author><name sortKey="Kalogeras, Kt" uniqKey="Kalogeras K">KT Kalogeras</name></author><author><name sortKey="Kouvatseas, G" uniqKey="Kouvatseas G">G Kouvatseas</name></author><author><name sortKey="Televantou, D" uniqKey="Televantou D">D Televantou</name></author><author><name sortKey="Kronenwett, R" uniqKey="Kronenwett R">R Kronenwett</name></author><author><name sortKey="Wirtz, Rm" uniqKey="Wirtz R">RM Wirtz</name></author><author><name sortKey="Fountzilas, G" uniqKey="Fountzilas G">G Fountzilas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dubsky, P" uniqKey="Dubsky P">P Dubsky</name></author><author><name sortKey="Filipits, M" uniqKey="Filipits M">M Filipits</name></author><author><name sortKey="Jakesz, R" uniqKey="Jakesz R">R Jakesz</name></author><author><name sortKey="Rudas, M" uniqKey="Rudas M">M Rudas</name></author><author><name sortKey="Singer, Cf" uniqKey="Singer C">CF Singer</name></author><author><name sortKey="Greil, R" uniqKey="Greil R">R Greil</name></author><author><name sortKey="Dietze, O" uniqKey="Dietze O">O Dietze</name></author><author><name sortKey="Luisser, I" uniqKey="Luisser I">I Luisser</name></author><author><name sortKey="Klug, E" uniqKey="Klug E">E Klug</name></author><author><name sortKey="Sedivy, R" uniqKey="Sedivy R">R Sedivy</name></author><author><name sortKey="Bachner, M" uniqKey="Bachner M">M Bachner</name></author><author><name sortKey="Mayr, D" uniqKey="Mayr D">D Mayr</name></author><author><name sortKey="Schmidt, M" uniqKey="Schmidt M">M Schmidt</name></author><author><name sortKey="Gehrmann, Mc" uniqKey="Gehrmann M">MC Gehrmann</name></author><author><name sortKey="Petry, C" uniqKey="Petry C">C Petry</name></author><author><name sortKey="Weber, Ke" uniqKey="Weber K">KE Weber</name></author><author><name sortKey="Kronenwett, R" uniqKey="Kronenwett R">R Kronenwett</name></author><author><name sortKey="Brase, Jc" uniqKey="Brase J">JC Brase</name></author><author><name sortKey="Gnant, M" uniqKey="Gnant M">M Gnant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simon, Rm" uniqKey="Simon R">RM Simon</name></author><author><name sortKey="Paik, S" uniqKey="Paik S">S Paik</name></author><author><name sortKey="Hayes, Df" uniqKey="Hayes D">DF Hayes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kronenwett, R" uniqKey="Kronenwett R">R Kronenwett</name></author><author><name sortKey="Bohmann, K" uniqKey="Bohmann K">K Bohmann</name></author><author><name sortKey="Prinzler, J" uniqKey="Prinzler J">J Prinzler</name></author><author><name sortKey="Sinn, Bv" uniqKey="Sinn B">BV Sinn</name></author><author><name sortKey="Haufe, F" uniqKey="Haufe F">F Haufe</name></author><author><name sortKey="Roth, C" uniqKey="Roth C">C Roth</name></author><author><name sortKey="Averdick, M" uniqKey="Averdick M">M Averdick</name></author><author><name sortKey="Ropers, T" uniqKey="Ropers T">T Ropers</name></author><author><name sortKey="Windbergs, C" uniqKey="Windbergs C">C Windbergs</name></author><author><name sortKey="Brase, Jc" uniqKey="Brase J">JC Brase</name></author><author><name sortKey="Weber, Ke" uniqKey="Weber K">KE Weber</name></author><author><name sortKey="Fisch, K" uniqKey="Fisch K">K Fisch</name></author><author><name sortKey="Muller, Bm" uniqKey="Muller B">BM Muller</name></author><author><name sortKey="Schmidt, M" uniqKey="Schmidt M">M Schmidt</name></author><author><name sortKey="Filipits, M" uniqKey="Filipits M">M Filipits</name></author><author><name sortKey="Dubsky, P" uniqKey="Dubsky P">P Dubsky</name></author><author><name sortKey="Petry, C" uniqKey="Petry C">C Petry</name></author><author><name sortKey="Dietel, M" uniqKey="Dietel M">M Dietel</name></author><author><name sortKey="Denkert, C" uniqKey="Denkert C">C Denkert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denkert, C" uniqKey="Denkert C">C Denkert</name></author><author><name sortKey="Kronenwett, R" uniqKey="Kronenwett R">R Kronenwett</name></author><author><name sortKey="Schlake, W" uniqKey="Schlake W">W Schlake</name></author><author><name sortKey="Bohmann, K" uniqKey="Bohmann K">K Bohmann</name></author><author><name sortKey="Penzel, R" uniqKey="Penzel R">R Penzel</name></author><author><name sortKey="Weber, Ke" uniqKey="Weber K">KE Weber</name></author><author><name sortKey="Hofler, H" uniqKey="Hofler H">H Hofler</name></author><author><name sortKey="Lehmann, U" uniqKey="Lehmann U">U Lehmann</name></author><author><name sortKey="Schirmacher, P" uniqKey="Schirmacher P">P Schirmacher</name></author><author><name sortKey="Specht, K" uniqKey="Specht K">K Specht</name></author><author><name sortKey="Rudas, M" uniqKey="Rudas M">M Rudas</name></author><author><name sortKey="Kreipe, Hh" uniqKey="Kreipe H">HH Kreipe</name></author><author><name sortKey="Schraml, P" uniqKey="Schraml P">P Schraml</name></author><author><name sortKey="Schlake, G" uniqKey="Schlake G">G Schlake</name></author><author><name sortKey="Bago Horvath, Z" uniqKey="Bago Horvath Z">Z Bago-Horvath</name></author><author><name sortKey="Tiecke, F" uniqKey="Tiecke F">F Tiecke</name></author><author><name sortKey="Varga, Z" uniqKey="Varga Z">Z Varga</name></author><author><name sortKey="Moch, H" uniqKey="Moch H">H Moch</name></author><author><name sortKey="Schmidt, M" uniqKey="Schmidt M">M Schmidt</name></author><author><name sortKey="Prinzler, J" uniqKey="Prinzler J">J Prinzler</name></author><author><name sortKey="Kerjaschki, D" uniqKey="Kerjaschki D">D Kerjaschki</name></author><author><name sortKey="Sinn, Bv" uniqKey="Sinn B">BV Sinn</name></author><author><name sortKey="Muller, Bm" uniqKey="Muller B">BM Muller</name></author><author><name sortKey="Filipits, M" uniqKey="Filipits M">M Filipits</name></author><author><name sortKey="Petry, C" uniqKey="Petry C">C Petry</name></author><author><name sortKey="Dietel, M" uniqKey="Dietel M">M Dietel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bohmann, K" uniqKey="Bohmann K">K Bohmann</name></author><author><name sortKey="Hennig, G" uniqKey="Hennig G">G Hennig</name></author><author><name sortKey="Rogel, U" uniqKey="Rogel U">U Rogel</name></author><author><name sortKey="Poremba, C" uniqKey="Poremba C">C Poremba</name></author><author><name sortKey="Mueller, Bm" uniqKey="Mueller B">BM Mueller</name></author><author><name sortKey="Fritz, P" uniqKey="Fritz P">P Fritz</name></author><author><name sortKey="Stoerkel, S" uniqKey="Stoerkel S">S Stoerkel</name></author><author><name sortKey="Schaefer, Kl" uniqKey="Schaefer K">KL Schaefer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hennig, G" uniqKey="Hennig G">G Hennig</name></author><author><name sortKey="Gehrmann, M" uniqKey="Gehrmann M">M Gehrmann</name></author><author><name sortKey="Stropp, U" uniqKey="Stropp U">U Stropp</name></author><author><name sortKey="Brauch, H" uniqKey="Brauch H">H Brauch</name></author><author><name sortKey="Fritz, P" uniqKey="Fritz P">P Fritz</name></author><author><name sortKey="Eichelbaum, M" uniqKey="Eichelbaum M">M Eichelbaum</name></author><author><name sortKey="Schwab, M" uniqKey="Schwab M">M Schwab</name></author><author><name sortKey="Schroth, W" uniqKey="Schroth W">W Schroth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Srinivasan, M" uniqKey="Srinivasan M">M Srinivasan</name></author><author><name sortKey="Sedmak, D" uniqKey="Sedmak D">D Sedmak</name></author><author><name sortKey="Jewell, S" uniqKey="Jewell S">S Jewell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chung, Jy" uniqKey="Chung J">JY Chung</name></author><author><name sortKey="Braunschweig, T" uniqKey="Braunschweig T">T Braunschweig</name></author><author><name sortKey="Williams, R" uniqKey="Williams R">R Williams</name></author><author><name sortKey="Guerrero, N" uniqKey="Guerrero N">N Guerrero</name></author><author><name sortKey="Hoffmann, Km" uniqKey="Hoffmann K">KM Hoffmann</name></author><author><name sortKey="Kwon, M" uniqKey="Kwon M">M Kwon</name></author><author><name sortKey="Song, Yk" uniqKey="Song Y">YK Song</name></author><author><name sortKey="Libutti, Sk" uniqKey="Libutti S">SK Libutti</name></author><author><name sortKey="Hewitt, Sm" uniqKey="Hewitt S">SM Hewitt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Godfrey, Te" uniqKey="Godfrey T">TE Godfrey</name></author><author><name sortKey="Kim, Sh" uniqKey="Kim S">SH Kim</name></author><author><name sortKey="Chavira, M" uniqKey="Chavira M">M Chavira</name></author><author><name sortKey="Ruff, Dw" uniqKey="Ruff D">DW Ruff</name></author><author><name sortKey="Warren, Rs" uniqKey="Warren R">RS Warren</name></author><author><name sortKey="Gray, Jw" uniqKey="Gray J">JW Gray</name></author><author><name sortKey="Jensen, Rh" uniqKey="Jensen R">RH Jensen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abrahamsen, Hn" uniqKey="Abrahamsen H">HN Abrahamsen</name></author><author><name sortKey="Steiniche, T" uniqKey="Steiniche T">T Steiniche</name></author><author><name sortKey="Nexo, E" uniqKey="Nexo E">E Nexo</name></author><author><name sortKey="Hamilton Dutoit, Sj" uniqKey="Hamilton Dutoit S">SJ Hamilton-Dutoit</name></author><author><name sortKey="Sorensen, Bs" uniqKey="Sorensen B">BS Sorensen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Cecco, L" uniqKey="De Cecco L">L De Cecco</name></author><author><name sortKey="Musella, V" uniqKey="Musella V">V Musella</name></author><author><name sortKey="Veneroni, S" uniqKey="Veneroni S">S Veneroni</name></author><author><name sortKey="Cappelletti, V" uniqKey="Cappelletti V">V Cappelletti</name></author><author><name sortKey="Bongarzone, I" uniqKey="Bongarzone I">I Bongarzone</name></author><author><name sortKey="Callari, M" uniqKey="Callari M">M Callari</name></author><author><name sortKey="Valeri, B" uniqKey="Valeri B">B Valeri</name></author><author><name sortKey="Pierotti, Ma" uniqKey="Pierotti M">MA Pierotti</name></author><author><name sortKey="Daidone, Mg" uniqKey="Daidone M">MG Daidone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muller, Bm" uniqKey="Muller B">BM Muller</name></author><author><name sortKey="Kronenwett, R" uniqKey="Kronenwett R">R Kronenwett</name></author><author><name sortKey="Hennig, G" uniqKey="Hennig G">G Hennig</name></author><author><name sortKey="Euting, H" uniqKey="Euting H">H Euting</name></author><author><name sortKey="Weber, K" uniqKey="Weber K">K Weber</name></author><author><name sortKey="Bohmann, K" uniqKey="Bohmann K">K Bohmann</name></author><author><name sortKey="Weichert, W" uniqKey="Weichert W">W Weichert</name></author><author><name sortKey="Altmann, G" uniqKey="Altmann G">G Altmann</name></author><author><name sortKey="Roth, C" uniqKey="Roth C">C Roth</name></author><author><name sortKey="Winzer, Kj" uniqKey="Winzer K">KJ Winzer</name></author><author><name sortKey="Kristiansen, G" uniqKey="Kristiansen G">G Kristiansen</name></author><author><name sortKey="Petry, C" uniqKey="Petry C">C Petry</name></author><author><name sortKey="Dietel, M" uniqKey="Dietel M">M Dietel</name></author><author><name sortKey="Denkert, C" uniqKey="Denkert C">C Denkert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Macabeo Ong, M" uniqKey="Macabeo Ong M">M Macabeo-Ong</name></author><author><name sortKey="Ginzinger, Dg" uniqKey="Ginzinger D">DG Ginzinger</name></author><author><name sortKey="Dekker, N" uniqKey="Dekker N">N Dekker</name></author><author><name sortKey="Mcmillan, A" uniqKey="Mcmillan A">A McMillan</name></author><author><name sortKey="Regezi, Ja" uniqKey="Regezi J">JA Regezi</name></author><author><name sortKey="Wong, Dt" uniqKey="Wong D">DT Wong</name></author><author><name sortKey="Jordan, Rc" uniqKey="Jordan R">RC Jordan</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Schobesberger, M" uniqKey="Schobesberger M">M Schobesberger</name></author><author><name sortKey="Baltzer, A" uniqKey="Baltzer A">A Baltzer</name></author><author><name sortKey="Oberli, A" uniqKey="Oberli A">A Oberli</name></author><author><name sortKey="Kappeler, A" uniqKey="Kappeler A">A Kappeler</name></author><author><name sortKey="Gugger, M" uniqKey="Gugger M">M Gugger</name></author><author><name sortKey="Burger, H" uniqKey="Burger H">H Burger</name></author><author><name sortKey="Jaggi, R" uniqKey="Jaggi R">R Jaggi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tramm, T" uniqKey="Tramm T">T Tramm</name></author><author><name sortKey="Hennig, G" uniqKey="Hennig G">G Hennig</name></author><author><name sortKey="Kyndi, M" uniqKey="Kyndi M">M Kyndi</name></author><author><name sortKey="Alsner, J" uniqKey="Alsner J">J Alsner</name></author><author><name sortKey="Sorensen, Fb" uniqKey="Sorensen F">FB Sorensen</name></author><author><name sortKey="Myhre, S" uniqKey="Myhre S">S Myhre</name></author><author><name sortKey="Sorlie, T" uniqKey="Sorlie T">T Sorlie</name></author><author><name sortKey="Overgaard, J" uniqKey="Overgaard J">J Overgaard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nielsen, T" uniqKey="Nielsen T">T Nielsen</name></author><author><name sortKey="Wallden, B" uniqKey="Wallden B">B Wallden</name></author><author><name sortKey="Schaper, C" uniqKey="Schaper C">C Schaper</name></author><author><name sortKey="Ferree, S" uniqKey="Ferree S">S Ferree</name></author><author><name sortKey="Liu, S" uniqKey="Liu S">S Liu</name></author><author><name sortKey="Gao, D" uniqKey="Gao D">D Gao</name></author><author><name sortKey="Barry, G" uniqKey="Barry G">G Barry</name></author><author><name sortKey="Dowidar, N" uniqKey="Dowidar N">N Dowidar</name></author><author><name sortKey="Maysuria, M" uniqKey="Maysuria M">M Maysuria</name></author><author><name sortKey="Storhoff, J" uniqKey="Storhoff J">J Storhoff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cronin, M" uniqKey="Cronin M">M Cronin</name></author><author><name sortKey="Pho, M" uniqKey="Pho M">M Pho</name></author><author><name sortKey="Dutta, D" uniqKey="Dutta D">D Dutta</name></author><author><name sortKey="Stephans, Jc" uniqKey="Stephans J">JC Stephans</name></author><author><name sortKey="Shak, S" uniqKey="Shak S">S Shak</name></author><author><name sortKey="Kiefer, Mc" uniqKey="Kiefer M">MC Kiefer</name></author><author><name sortKey="Esteban, Jm" uniqKey="Esteban J">JM Esteban</name></author><author><name sortKey="Baker, Jb" uniqKey="Baker J">JB Baker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Von Ahlfen, S" uniqKey="Von Ahlfen S">S von Ahlfen</name></author><author><name sortKey="Missel, A" uniqKey="Missel A">A Missel</name></author><author><name sortKey="Bendrat, K" uniqKey="Bendrat K">K Bendrat</name></author><author><name sortKey="Schlumpberger, M" uniqKey="Schlumpberger M">M Schlumpberger</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Bavière</li><li>Berlin</li><li>District de Cologne</li><li>District de Düsseldorf</li><li>District de Haute-Bavière</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Berlin</li><li>Cologne</li><li>Düsseldorf</li><li>Munich</li></settlement></list><tree><country name="Allemagne"><region name="Bavière"><name sortKey="Poremba, Christopher" sort="Poremba, Christopher" uniqKey="Poremba C" first="Christopher" last="Poremba">Christopher Poremba</name></region><name sortKey="Averdick, Manuela" sort="Averdick, Manuela" uniqKey="Averdick M" first="Manuela" last="Averdick">Manuela Averdick</name><name sortKey="Bohmann, Kerstin" sort="Bohmann, Kerstin" uniqKey="Bohmann K" first="Kerstin" last="Bohmann">Kerstin Bohmann</name><name sortKey="Bojar, Hans" sort="Bojar, Hans" uniqKey="Bojar H" first="Hans" last="Bojar">Hans Bojar</name><name sortKey="Brase, Jan C" sort="Brase, Jan C" uniqKey="Brase J" first="Jan C." last="Brase">Jan C. Brase</name><name sortKey="Denkert, Carsten" sort="Denkert, Carsten" uniqKey="Denkert C" first="Carsten" last="Denkert">Carsten Denkert</name><name sortKey="Dietel, Manfred" sort="Dietel, Manfred" uniqKey="Dietel M" first="Manfred" last="Dietel">Manfred Dietel</name><name sortKey="Haufe, Franziska" sort="Haufe, Franziska" uniqKey="Haufe F" first="Franziska" last="Haufe">Franziska Haufe</name><name sortKey="Hennig, Guido" sort="Hennig, Guido" uniqKey="Hennig G" first="Guido" last="Hennig">Guido Hennig</name><name sortKey="Krenn, Veit" sort="Krenn, Veit" uniqKey="Krenn V" first="Veit" last="Krenn">Veit Krenn</name><name sortKey="Kronenwett, Ralf" sort="Kronenwett, Ralf" uniqKey="Kronenwett R" first="Ralf" last="Kronenwett">Ralf Kronenwett</name><name sortKey="Kronenwett, Ralf" sort="Kronenwett, Ralf" uniqKey="Kronenwett R" first="Ralf" last="Kronenwett">Ralf Kronenwett</name><name sortKey="Pfitzner, Berit M" sort="Pfitzner, Berit M" uniqKey="Pfitzner B" first="Berit M." last="Pfitzner">Berit M. Pfitzner</name><name sortKey="Poremba, Christopher" sort="Poremba, Christopher" uniqKey="Poremba C" first="Christopher" last="Poremba">Christopher Poremba</name><name sortKey="Uhlendorff, Jennifer" sort="Uhlendorff, Jennifer" uniqKey="Uhlendorff J" first="Jennifer" last="Uhlendorff">Jennifer Uhlendorff</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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