Detection of estrogen-like compounds by thin-layer chromatography
Identifieur interne : 000931 ( Istex/Corpus ); précédent : 000930; suivant : 000932Detection of estrogen-like compounds by thin-layer chromatography
Auteurs : Birgit Wortberg ; Richard WollerSource :
- Journal of Chromatography A [ 0021-9673 ] ; 1978.
Abstract
There are different national food regulations concerning the use of hormone-active substances in the breeding of domestic animals that serve as human food. This situation requires specific and sensitive methods for the detection of any residues in food. The hormones used in practice can be determined with improved sensitivity by coupling to the diazonium compound Fast Dark Blue R Salt. After thin-layer chromatography on high-performance thin-layer chromatographic plates with two different solvent mixtures, zeranol, estradiol, estriol and estrone are analyzed as azo dyestuffs. The detection limit of these hormones is below 10 ng.
Url:
DOI: 10.1016/S0021-9673(00)83141-6
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This situation requires specific and sensitive methods for the detection of any residues in food. The hormones used in practice can be determined with improved sensitivity by coupling to the diazonium compound Fast Dark Blue R Salt. After thin-layer chromatography on high-performance thin-layer chromatographic plates with two different solvent mixtures, zeranol, estradiol, estriol and estrone are analyzed as azo dyestuffs. 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Chem.</title></host></json:item><json:item><host><author></author></host></json:item><json:item><host><author></author></host></json:item><json:item><host><author></author></host></json:item><json:item><host><author></author></host></json:item><json:item><host><volume>56</volume><pages><first>1326</first></pages><author></author><title>Fleischwirtschaft</title></host></json:item><json:item><host><volume>3</volume><pages><first>3</first></pages><author></author><title>Toxicology</title></host></json:item><json:item><host><volume>23</volume><pages><first>70</first></pages><author></author><title>Arch. 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Chem.</title></host></json:item><json:item><host><volume>157</volume><pages><first>189</first></pages><author></author><title>Z. Lebensm.-Unters.-Forsch.</title></host></json:item></refBibs><genre><json:string>research-article</json:string></genre><host><volume>156</volume><pii><json:string>S0021-9673(00)X0376-7</json:string></pii><pages><last>210</last><first>205</first></pages><issn><json:string>0021-9673</json:string></issn><issue>1</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Journal of Chromatography A</title><publicationDate>1978</publicationDate></host><categories><wos><json:string>science</json:string><json:string>chemistry, analytical</json:string><json:string>biochemical research methods</json:string></wos><scienceMetrix><json:string>natural sciences</json:string><json:string>chemistry</json:string><json:string>analytical chemistry</json:string></scienceMetrix></categories><publicationDate>1978</publicationDate><copyrightDate>1978</copyrightDate><doi><json:string>10.1016/S0021-9673(00)83141-6</json:string></doi><id>D6684456834F2BDEECDE3FE0130023E5C66372E5</id><score>0.54624265</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/D6684456834F2BDEECDE3FE0130023E5C66372E5/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/D6684456834F2BDEECDE3FE0130023E5C66372E5/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/D6684456834F2BDEECDE3FE0130023E5C66372E5/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Detection of estrogen-like compounds by thin-layer chromatography</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1978</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Detection of estrogen-like compounds by thin-layer chromatography</title><author xml:id="author-1"><persName><forename type="first">Birgit</forename><surname>Wortberg</surname></persName><affiliation>Chemisches Untersuchungsamt Trier, Maximineracht 11a, 5500 Trier G.F.R.Ministry of Health, Department of Medical Science, Division of Food Analysis, Yod-Se, Bangkok Thailand</affiliation></author><author xml:id="author-2"><persName><forename type="first">Richard</forename><surname>Woller</surname></persName><affiliation>Chemisches Untersuchungsamt Trier, Maximineracht 11a, 5500 Trier G.F.R.Ministry of Health, Department of Medical Science, Division of Food Analysis, Yod-Se, Bangkok Thailand</affiliation></author></analytic><monogr><title level="j">Journal of Chromatography A</title><title level="j" type="abbrev">CHROMA</title><idno type="pISSN">0021-9673</idno><idno type="PII">S0021-9673(00)X0376-7</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1978"></date><biblScope unit="volume">156</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="205">205</biblScope><biblScope unit="page" to="210">210</biblScope></imprint></monogr><idno type="istex">D6684456834F2BDEECDE3FE0130023E5C66372E5</idno><idno type="DOI">10.1016/S0021-9673(00)83141-6</idno><idno type="PII">S0021-9673(00)83141-6</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1978</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>There are different national food regulations concerning the use of hormone-active substances in the breeding of domestic animals that serve as human food. 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This situation requires specific and sensitive methods for the detection of any residues in food. The hormones used in practice can be determined with improved sensitivity by coupling to the diazonium compound Fast Dark Blue R Salt. After thin-layer chromatography on high-performance thin-layer chromatographic plates with two different solvent mixtures, zeranol, estradiol, estriol and estrone are analyzed as azo dyestuffs. 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