A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum
Identifieur interne : 000700 ( Istex/Corpus ); précédent : 000699; suivant : 000701A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum
Auteurs : Marian Casn ; Dieter Rehder ; Hauke Schmidt ; Hans Vilter ; Valeria ConteSource :
- Journal of Inorganic Biochemistry [ 0162-0134 ] ; 2000.
English descriptors
- KwdEn :
Abstract
The 17O NMR of bromoperoxidase in Tris buffer at pH 8 treated with 17O-enriched H2O2 reveals direct binding of peroxide to active site vanadium both in the symmetric and asymmetric modes, the latter possibly due to hydroperoxide. In addition, non-active site HVO2(O2)22− is detected. The results are counter-checked with NMR data on peroxovanadium model compounds.
Url:
DOI: 10.1016/S0162-0134(00)00024-6
Links to Exploration step
ISTEX:6BF503D28AE460052A3A9044728F890FA9A4A57BLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum</title><author><name sortKey="Casn, Marian" sort="Casn, Marian" uniqKey="Casn M" first="Marian" last="Casn">Marian Casn</name><affiliation><mods:affiliation>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Rehder, Dieter" sort="Rehder, Dieter" uniqKey="Rehder D" first="Dieter" last="Rehder">Dieter Rehder</name><affiliation><mods:affiliation>E-mail: rehder@xray.chemie.uni-hamburg.de</mods:affiliation></affiliation><affiliation><mods:affiliation>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schmidt, Hauke" sort="Schmidt, Hauke" uniqKey="Schmidt H" first="Hauke" last="Schmidt">Hauke Schmidt</name><affiliation><mods:affiliation>Avdelningen för Oorganisk Kemi, Umeå Universitet, S-90187 Umeå, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Vilter, Hans" sort="Vilter, Hans" uniqKey="Vilter H" first="Hans" last="Vilter">Hans Vilter</name><affiliation><mods:affiliation>Zurmaienstraße 16, D-54292 Trier, Germany</mods:affiliation></affiliation></author><author><name sortKey="Conte, Valeria" sort="Conte, Valeria" uniqKey="Conte V" first="Valeria" last="Conte">Valeria Conte</name><affiliation><mods:affiliation>Facoltà di Agraria II, Foggia, Università di Bari, I-71100 Foggia, Italy</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:6BF503D28AE460052A3A9044728F890FA9A4A57B</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1016/S0162-0134(00)00024-6</idno><idno type="url">https://api.istex.fr/document/6BF503D28AE460052A3A9044728F890FA9A4A57B/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000700</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000700</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum</title><author><name sortKey="Casn, Marian" sort="Casn, Marian" uniqKey="Casn M" first="Marian" last="Casn">Marian Casn</name><affiliation><mods:affiliation>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Rehder, Dieter" sort="Rehder, Dieter" uniqKey="Rehder D" first="Dieter" last="Rehder">Dieter Rehder</name><affiliation><mods:affiliation>E-mail: rehder@xray.chemie.uni-hamburg.de</mods:affiliation></affiliation><affiliation><mods:affiliation>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schmidt, Hauke" sort="Schmidt, Hauke" uniqKey="Schmidt H" first="Hauke" last="Schmidt">Hauke Schmidt</name><affiliation><mods:affiliation>Avdelningen för Oorganisk Kemi, Umeå Universitet, S-90187 Umeå, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Vilter, Hans" sort="Vilter, Hans" uniqKey="Vilter H" first="Hans" last="Vilter">Hans Vilter</name><affiliation><mods:affiliation>Zurmaienstraße 16, D-54292 Trier, Germany</mods:affiliation></affiliation></author><author><name sortKey="Conte, Valeria" sort="Conte, Valeria" uniqKey="Conte V" first="Valeria" last="Conte">Valeria Conte</name><affiliation><mods:affiliation>Facoltà di Agraria II, Foggia, Università di Bari, I-71100 Foggia, Italy</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Inorganic Biochemistry</title><title level="j" type="abbrev">JIB</title><idno type="ISSN">0162-0134</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">80</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="157">157</biblScope><biblScope unit="page" to="160">160</biblScope></imprint><idno type="ISSN">0162-0134</idno></series><idno type="istex">6BF503D28AE460052A3A9044728F890FA9A4A57B</idno><idno type="DOI">10.1016/S0162-0134(00)00024-6</idno><idno type="PII">S0162-0134(00)00024-6</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0162-0134</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>17O NMR</term><term>Bromoperoxidase</term><term>Hydroperoxide</term><term>Peroxovanadium</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The 17O NMR of bromoperoxidase in Tris buffer at pH 8 treated with 17O-enriched H2O2 reveals direct binding of peroxide to active site vanadium both in the symmetric and asymmetric modes, the latter possibly due to hydroperoxide. In addition, non-active site HVO2(O2)22− is detected. The results are counter-checked with NMR data on peroxovanadium model compounds.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Marian Casný</name><affiliations><json:string>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</json:string></affiliations></json:item><json:item><name>Dieter Rehder</name><affiliations><json:string>E-mail: rehder@xray.chemie.uni-hamburg.de</json:string><json:string>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</json:string></affiliations></json:item><json:item><name>Hauke Schmidt</name><affiliations><json:string>Avdelningen för Oorganisk Kemi, Umeå Universitet, S-90187 Umeå, Sweden</json:string></affiliations></json:item><json:item><name>Hans Vilter</name><affiliations><json:string>Zurmaienstraße 16, D-54292 Trier, Germany</json:string></affiliations></json:item><json:item><name>Valeria Conte</name><affiliations><json:string>Facoltà di Agraria II, Foggia, Università di Bari, I-71100 Foggia, Italy</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Bromoperoxidase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Peroxovanadium</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Hydroperoxide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>17O NMR</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Short communication</json:string></originalGenre><abstract>The 17O NMR of bromoperoxidase in Tris buffer at pH 8 treated with 17O-enriched H2O2 reveals direct binding of peroxide to active site vanadium both in the symmetric and asymmetric modes, the latter possibly due to hydroperoxide. In addition, non-active site HVO2(O2)22− is detected. The results are counter-checked with NMR data on peroxovanadium model compounds.</abstract><qualityIndicators><score>2.91</score><pdfVersion>1.2</pdfVersion><pdfPageSize>591 x 813 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>4</keywordCount><abstractCharCount>364</abstractCharCount><pdfWordCount>2250</pdfWordCount><pdfCharCount>14034</pdfCharCount><pdfPageCount>4</pdfPageCount><abstractWordCount>55</abstractWordCount></qualityIndicators><title>A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum</title><pii><json:string>S0162-0134(00)00024-6</json:string></pii><refBibs><json:item><host><volume>89</volume><pages><first>109</first></pages><author></author><title>Struct. Bonding</title></host></json:item><json:item><host><volume>293</volume><pages><first>595</first></pages><author></author><title>J. Mol. Biol.</title></host></json:item><json:item><host><volume>378</volume><pages><first>309</first></pages><author></author><title>Biol. Chem.</title></host></json:item><json:item><host><volume>4</volume><pages><first>209</first></pages><author></author><title>J. Biol. Inorg. Chem.</title></host></json:item><json:item><host><volume>116</volume><pages><first>411</first></pages><author></author><title>J. Am. Chem. Soc.</title></host></json:item><json:item><host><volume>457</volume><pages><first>237</first></pages><author></author><title>FEBS Lett.</title></host></json:item><json:item><host><volume>118</volume><pages><first>3469</first></pages><author></author><title>J. Am. Chem. Soc.</title></host></json:item><json:item><host><volume>37</volume><pages><first>949</first></pages><author></author><title>Inorg. Chem.</title></host></json:item><json:item><host><volume>265</volume><pages><first>4908</first></pages><author></author><title>J. Biol. Chem.</title></host></json:item><json:item><host><volume>117</volume><pages><first>3475</first></pages><author></author><title>J. Am. Chem. Soc.</title></host></json:item><json:item><host><author></author></host></json:item><json:item><host><volume>7</volume><pages><first>31</first></pages><author></author><title>Chim. Oggi</title></host></json:item><json:item><host><volume>28</volume><pages><first>2458</first></pages><author></author><title>J. Inorg. Nucl. Chem.</title></host></json:item><json:item><host><volume>119</volume><pages><first>5447</first></pages><author></author><title>J. Am. Chem. Soc.</title></host></json:item><json:item><host><volume>12</volume><pages><first>829</first></pages><author></author><title>Inorg. Chem.</title></host></json:item><json:item><host><volume>18</volume><pages><first>304</first></pages><author></author><title>Transition Met. Chem.</title></host></json:item><json:item><host><author></author></host></json:item><json:item><host><author></author></host></json:item><json:item><host><author></author></host></json:item><json:item><host><author></author></host></json:item><json:item><host><author></author></host></json:item><json:item><host><volume>9</volume><pages><last>632</last><first>631</first></pages><author></author><title>Chem. Listy</title></host></json:item><json:item><host><volume>44</volume><pages><first>865</first></pages><author></author><title>Helv. Chim. Acta</title></host></json:item><json:item><host><volume>21</volume><pages><first>458</first></pages><author></author><title>Quart. Rev. Chem. Soc.</title></host></json:item><json:item><host><author></author></host></json:item><json:item><host><volume>6</volume><pages><first>1813</first></pages><author></author><title>Polyhedron</title></host></json:item><json:item><host><volume>41</volume><pages><first>171</first></pages><author></author><title>J. Inorg. Biochem.</title></host></json:item><json:item><host><volume>A38</volume><pages><first>101</first></pages><author></author><title>Acta Chem. Scand.</title></host></json:item><json:item><host><volume>7</volume><pages><first>467</first></pages><author></author><title>Nouv. J. Chim.</title></host></json:item><json:item><host><volume>16</volume><pages><first>2837</first></pages><author></author><title>Polyhedron</title></host></json:item><json:item><host><volume>29</volume><pages><first>4457</first></pages><author></author><title>Inorg. Chem.</title></host></json:item><json:item><host><author></author></host></json:item><json:item><host><volume>120</volume><pages><first>93</first></pages><author></author><title>J. Mol. Catal.</title></host></json:item></refBibs><genre><json:string>brief-communication</json:string></genre><host><volume>80</volume><pii><json:string>S0162-0134(00)X0067-0</json:string></pii><pages><last>160</last><first>157</first></pages><issn><json:string>0162-0134</json:string></issn><issue>1–2</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><title>Journal of Inorganic Biochemistry</title><publicationDate>2000</publicationDate></host><categories><wos><json:string>science</json:string><json:string>chemistry, inorganic & nuclear</json:string><json:string>biochemistry & molecular biology</json:string></wos><scienceMetrix><json:string>natural sciences</json:string><json:string>chemistry</json:string><json:string>inorganic & nuclear chemistry</json:string></scienceMetrix></categories><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1016/S0162-0134(00)00024-6</json:string></doi><id>6BF503D28AE460052A3A9044728F890FA9A4A57B</id><score>0.28421965</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/6BF503D28AE460052A3A9044728F890FA9A4A57B/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/6BF503D28AE460052A3A9044728F890FA9A4A57B/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/6BF503D28AE460052A3A9044728F890FA9A4A57B/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>©2000 Elsevier Science Inc.</p></availability><date>2000</date></publicationStmt><notesStmt><note type="content">Section title: Short Communication</note><note type="content">Fig. 1: 48.82 MHz 17O NMR spectrum of AnPO (0.43 mM) in Tris buffer at pH 8, treated with 17O-enriched (45%) H2O2. For the assignment of peroxovanadium signals see also Table 1 and text.</note><note type="content">Fig. 2: 48.82 MHz 17O NMR spectra of [VO2nta]2− in aqueous solution treated with excess H2(17O)2 (top), and (bottom) of the quarternary system H+–vanadate–alanylhistidine–peroxide (for details see footnote b of Table 1).</note><note type="content">Scheme 1:</note><note type="content">Table 1: NMR shift parametersa (main signal(s) only) and assignments</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum</title><author xml:id="author-1"><persName><forename type="first">Marian</forename><surname>Casný</surname></persName><affiliation>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</affiliation></author><author xml:id="author-2"><persName><forename type="first">Dieter</forename><surname>Rehder</surname></persName><email>rehder@xray.chemie.uni-hamburg.de</email><note type="correspondence"><p>Corresponding author. Tel.: +49-40-42838-6087; fax: +49-40-42838-2893</p></note><affiliation>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</affiliation></author><author xml:id="author-3"><persName><forename type="first">Hauke</forename><surname>Schmidt</surname></persName><affiliation>Avdelningen för Oorganisk Kemi, Umeå Universitet, S-90187 Umeå, Sweden</affiliation></author><author xml:id="author-4"><persName><forename type="first">Hans</forename><surname>Vilter</surname></persName><affiliation>Zurmaienstraße 16, D-54292 Trier, Germany</affiliation></author><author xml:id="author-5"><persName><forename type="first">Valeria</forename><surname>Conte</surname></persName><affiliation>Facoltà di Agraria II, Foggia, Università di Bari, I-71100 Foggia, Italy</affiliation></author></analytic><monogr><title level="j">Journal of Inorganic Biochemistry</title><title level="j" type="abbrev">JIB</title><idno type="pISSN">0162-0134</idno><idno type="PII">S0162-0134(00)X0067-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000"></date><biblScope unit="volume">80</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="157">157</biblScope><biblScope unit="page" to="160">160</biblScope></imprint></monogr><idno type="istex">6BF503D28AE460052A3A9044728F890FA9A4A57B</idno><idno type="DOI">10.1016/S0162-0134(00)00024-6</idno><idno type="PII">S0162-0134(00)00024-6</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The 17O NMR of bromoperoxidase in Tris buffer at pH 8 treated with 17O-enriched H2O2 reveals direct binding of peroxide to active site vanadium both in the symmetric and asymmetric modes, the latter possibly due to hydroperoxide. In addition, non-active site HVO2(O2)22− is detected. The results are counter-checked with NMR data on peroxovanadium model compounds.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Bromoperoxidase</term></item><item><term>Peroxovanadium</term></item><item><term>Hydroperoxide</term></item><item><term>17O NMR</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2000">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/6BF503D28AE460052A3A9044728F890FA9A4A57B/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2" NDATA="IMAGE" name="gr2"></istex:entity><istex:entity SYSTEM="sc1" NDATA="IMAGE" name="sc1"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="sco" xml:lang="en"><item-info><jid>JIB</jid><aid>6367</aid><ce:pii>S0162-0134(00)00024-6</ce:pii><ce:doi>10.1016/S0162-0134(00)00024-6</ce:doi><ce:copyright type="full-transfer" year="2000">Elsevier Science Inc.</ce:copyright></item-info><head><ce:dochead><ce:textfn>Short Communication</ce:textfn></ce:dochead><ce:title>A <ce:sup loc="pre">17</ce:sup>O NMR study of peroxide binding to the active centre of bromoperoxidase from <ce:italic>Ascophyllum nodosum</ce:italic></ce:title><ce:author-group><ce:author><ce:given-name>Marian</ce:given-name><ce:surname>Casný</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Dieter</ce:given-name><ce:surname>Rehder</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref><ce:e-address>rehder@xray.chemie.uni-hamburg.de</ce:e-address></ce:author><ce:author><ce:given-name>Hauke</ce:given-name><ce:surname>Schmidt</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Hans</ce:given-name><ce:surname>Vilter</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>c</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Valeria</ce:given-name><ce:surname>Conte</ce:surname><ce:cross-ref refid="AFF4"><ce:sup>d</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Avdelningen för Oorganisk Kemi, Umeå Universitet, S-90187 Umeå, Sweden</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Zurmaienstraße 16, D-54292 Trier, Germany</ce:textfn></ce:affiliation><ce:affiliation id="AFF4"><ce:label>d</ce:label><ce:textfn>Facoltà di Agraria II, Foggia, Università di Bari, I-71100 Foggia, Italy</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Corresponding author. Tel.: +49-40-42838-6087; fax: +49-40-42838-2893</ce:text></ce:correspondence></ce:author-group><ce:date-received day="29" month="9" year="1999"></ce:date-received><ce:date-accepted day="13" month="12" year="1999"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The <ce:sup loc="pre">17</ce:sup>O NMR of bromoperoxidase in Tris buffer at pH 8 treated with <ce:sup loc="pre">17</ce:sup>O-enriched H<ce:inf>2</ce:inf>O<ce:inf>2</ce:inf> reveals direct binding of peroxide to active site vanadium both in the symmetric and asymmetric modes, the latter possibly due to hydroperoxide. In addition, non-active site HVO<ce:inf>2</ce:inf>(O<ce:inf>2</ce:inf>)<ce:inf>2</ce:inf><ce:sup>2−</ce:sup> is detected. The results are counter-checked with NMR data on peroxovanadium model compounds.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="keyword"><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Bromoperoxidase</ce:text></ce:keyword><ce:keyword><ce:text>Peroxovanadium</ce:text></ce:keyword><ce:keyword><ce:text>Hydroperoxide</ce:text></ce:keyword><ce:keyword><ce:text><ce:sup>17</ce:sup>O NMR</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>A</title></titleInfo><name type="personal"><namePart type="given">Marian</namePart><namePart type="family">Casný</namePart><affiliation>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dieter</namePart><namePart type="family">Rehder</namePart><affiliation>E-mail: rehder@xray.chemie.uni-hamburg.de</affiliation><affiliation>Institut für Anorganische und Angewandte Chemie, Universität Hamburg, D-20146 Hamburg, Germany</affiliation><description>Corresponding author. Tel.: +49-40-42838-6087; fax: +49-40-42838-2893</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hauke</namePart><namePart type="family">Schmidt</namePart><affiliation>Avdelningen för Oorganisk Kemi, Umeå Universitet, S-90187 Umeå, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hans</namePart><namePart type="family">Vilter</namePart><affiliation>Zurmaienstraße 16, D-54292 Trier, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Valeria</namePart><namePart type="family">Conte</namePart><affiliation>Facoltà di Agraria II, Foggia, Università di Bari, I-71100 Foggia, Italy</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="Short communication"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The 17O NMR of bromoperoxidase in Tris buffer at pH 8 treated with 17O-enriched H2O2 reveals direct binding of peroxide to active site vanadium both in the symmetric and asymmetric modes, the latter possibly due to hydroperoxide. In addition, non-active site HVO2(O2)22− is detected. The results are counter-checked with NMR data on peroxovanadium model compounds.</abstract><note type="content">Section title: Short Communication</note><note type="content">Fig. 1: 48.82 MHz 17O NMR spectrum of AnPO (0.43 mM) in Tris buffer at pH 8, treated with 17O-enriched (45%) H2O2. For the assignment of peroxovanadium signals see also Table 1 and text.</note><note type="content">Fig. 2: 48.82 MHz 17O NMR spectra of [VO2nta]2− in aqueous solution treated with excess H2(17O)2 (top), and (bottom) of the quarternary system H+–vanadate–alanylhistidine–peroxide (for details see footnote b of Table 1).</note><note type="content">Scheme 1: </note><note type="content">Table 1: NMR shift parametersa (main signal(s) only) and assignments</note><subject lang="en"><genre>Keywords</genre><topic>Bromoperoxidase</topic><topic>Peroxovanadium</topic><topic>Hydroperoxide</topic><topic>17O NMR</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Inorganic Biochemistry</title></titleInfo><titleInfo type="abbreviated"><title>JIB</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">20000530</dateIssued></originInfo><identifier type="ISSN">0162-0134</identifier><identifier type="PII">S0162-0134(00)X0067-0</identifier><part><date>20000530</date><detail type="volume"><number>80</number><caption>vol.</caption></detail><detail type="issue"><number>1–2</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>194</end></extent><extent unit="pages"><start>157</start><end>160</end></extent></part></relatedItem><identifier type="istex">6BF503D28AE460052A3A9044728F890FA9A4A57B</identifier><identifier type="DOI">10.1016/S0162-0134(00)00024-6</identifier><identifier type="PII">S0162-0134(00)00024-6</identifier><accessCondition type="use and reproduction" contentType="copyright">©2000 Elsevier Science Inc.</accessCondition><recordInfo><recordContentSource>ELSEVIER</recordContentSource><recordOrigin>Elsevier Science Inc., ©2000</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Rhénanie/explor/UnivTrevesV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000700 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000700 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Rhénanie
|area= UnivTrevesV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:6BF503D28AE460052A3A9044728F890FA9A4A57B
|texte= A 17O NMR study of peroxide binding to the active centre of bromoperoxidase from Ascophyllum nodosum
}}
| This area was generated with Dilib version V0.6.31. |  |