Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores
Identifieur interne : 000801 ( Istex/Corpus ); précédent : 000800; suivant : 000802Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores
Auteurs : Mark E. Stearns ; Lester I. BinderSource :
- Cell Motility and the Cytoskeleton [ 0886-1544 ] ; 1987.
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Abstract
We have demonstrated the presence of MAP‐2 in squirrel fish erythrophores using SDS‐PAGE, immunobolt, and immunoprecipitation techniques. The monoclonal antibodies used (AP‐9, ‐13, ‐14) were raised against distinct antigenic sites on Chinese hamster brain MAP‐2. Immunoprecipitation studies demonstrated that all three antibodies bind a 300 K protein found in crude cell extracts and in partially purified MAP fractions isolated from erythrophores of the squirrel fish Holocentrus rufus. Immunofluorescent studies confirmed that the 300 K protein was present in cultured erythrophores. Studies of cells induced to aggregate and disperse their pigment granules revealed that the 300 K protein comigrated with the pigment, suggesting that the 300 K protein may constitute part of the “α‐cytomatrix” involved in pigment translocations.
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DOI: 10.1002/cm.970070305
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Binder</name><affiliation><mods:affiliation>Department of Anatomy, University of Alabama, Birmingham</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:605D07F4FE8ABA8A82AFAC65244643CA9C2D66A7</idno><date when="1987" year="1987">1987</date><idno type="doi">10.1002/cm.970070305</idno><idno type="url">https://api.istex.fr/document/605D07F4FE8ABA8A82AFAC65244643CA9C2D66A7/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000801</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores</title><author><name sortKey="Stearns, Mark E" sort="Stearns, Mark E" uniqKey="Stearns M" first="Mark E." last="Stearns">Mark E. Stearns</name><affiliation><mods:affiliation>Department of Pharmacology, Fox Chase Cancer Center, Philadelphia</mods:affiliation></affiliation></author><author><name sortKey="Binder, Lester I" sort="Binder, Lester I" uniqKey="Binder L" first="Lester I." last="Binder">Lester I. Binder</name><affiliation><mods:affiliation>Department of Anatomy, University of Alabama, Birmingham</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell Motility and the Cytoskeleton</title><title level="j" type="abbrev">Cell Motil. Cytoskeleton</title><idno type="ISSN">0886-1544</idno><idno type="eISSN">1097-0169</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1987">1987</date><biblScope unit="volume">7</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="221">221</biblScope><biblScope unit="page" to="234">234</biblScope></imprint><idno type="ISSN">0886-1544</idno></series><idno type="istex">605D07F4FE8ABA8A82AFAC65244643CA9C2D66A7</idno><idno type="DOI">10.1002/cm.970070305</idno><idno type="ArticleID">CM970070305</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0886-1544</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>immnuolabeling</term><term>monoclonal antibodies</term><term>α‐cytomatrix</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have demonstrated the presence of MAP‐2 in squirrel fish erythrophores using SDS‐PAGE, immunobolt, and immunoprecipitation techniques. The monoclonal antibodies used (AP‐9, ‐13, ‐14) were raised against distinct antigenic sites on Chinese hamster brain MAP‐2. Immunoprecipitation studies demonstrated that all three antibodies bind a 300 K protein found in crude cell extracts and in partially purified MAP fractions isolated from erythrophores of the squirrel fish Holocentrus rufus. Immunofluorescent studies confirmed that the 300 K protein was present in cultured erythrophores. Studies of cells induced to aggregate and disperse their pigment granules revealed that the 300 K protein comigrated with the pigment, suggesting that the 300 K protein may constitute part of the “α‐cytomatrix” involved in pigment translocations.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Mark E. Stearns</name><affiliations><json:string>Department of Pharmacology, Fox Chase Cancer Center, Philadelphia</json:string></affiliations></json:item><json:item><name>Lester I. 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Immunoprecipitation studies demonstrated that all three antibodies bind a 300 K protein found in crude cell extracts and in partially purified MAP fractions isolated from erythrophores of the squirrel fish Holocentrus rufus. Immunofluorescent studies confirmed that the 300 K protein was present in cultured erythrophores. Studies of cells induced to aggregate and disperse their pigment granules revealed that the 300 K protein comigrated with the pigment, suggesting that the 300 K protein may constitute part of the “α‐cytomatrix” involved in pigment translocations.</abstract><qualityIndicators><score>6.404</score><pdfVersion>1.3</pdfVersion><pdfPageSize>594 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>3</keywordCount><abstractCharCount>832</abstractCharCount><pdfWordCount>6579</pdfWordCount><pdfCharCount>41127</pdfCharCount><pdfPageCount>14</pdfPageCount><abstractWordCount>117</abstractWordCount></qualityIndicators><title>Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores</title><genre><json:string>article</json:string></genre><host><volume>7</volume><publisherId><json:string>CM</json:string></publisherId><pages><total>14</total><last>234</last><first>221</first></pages><issn><json:string>0886-1544</json:string></issn><issue>3</issue><subject><json:item><value>Article</value></json:item></subject><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1097-0169</json:string></eissn><title>Cell Motility and the Cytoskeleton</title><doi><json:string>10.1002/(ISSN)1097-0169</json:string></doi></host><publicationDate>1987</publicationDate><copyrightDate>1987</copyrightDate><doi><json:string>10.1002/cm.970070305</json:string></doi><id>605D07F4FE8ABA8A82AFAC65244643CA9C2D66A7</id><score>0.022802342</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/605D07F4FE8ABA8A82AFAC65244643CA9C2D66A7/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/605D07F4FE8ABA8A82AFAC65244643CA9C2D66A7/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/605D07F4FE8ABA8A82AFAC65244643CA9C2D66A7/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><availability><p>Copyright © 1987 Wiley‐Liss, Inc.</p></availability><date>1987</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores</title><author xml:id="author-1"><persName><forename type="first">Mark E.</forename><surname>Stearns</surname></persName><note type="correspondence"><p>Correspondence: Department of Pharmacology, Fox Chase Cancer Center, 7701 Burholme Ave., Philadelphia, PA 19111</p></note><affiliation>Department of Pharmacology, Fox Chase Cancer Center, Philadelphia</affiliation></author><author xml:id="author-2"><persName><forename type="first">Lester I.</forename><surname>Binder</surname></persName><affiliation>Department of Anatomy, University of Alabama, Birmingham</affiliation></author></analytic><monogr><title level="j">Cell Motility and the Cytoskeleton</title><title level="j" type="abbrev">Cell Motil. 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The monoclonal antibodies used (AP‐9, ‐13, ‐14) were raised against distinct antigenic sites on Chinese hamster brain MAP‐2. Immunoprecipitation studies demonstrated that all three antibodies bind a 300 K protein found in crude cell extracts and in partially purified MAP fractions isolated from erythrophores of the squirrel fish Holocentrus rufus. Immunofluorescent studies confirmed that the 300 K protein was present in cultured erythrophores. 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The monoclonal antibodies used (AP‐9, ‐13, ‐14) were raised against distinct antigenic sites on Chinese hamster brain MAP‐2. Immunoprecipitation studies demonstrated that all three antibodies bind a 300 K protein found in crude cell extracts and in partially purified MAP fractions isolated from erythrophores of the squirrel fish <i>Holocentrus rufus</i>. Immunofluorescent studies confirmed that the 300 K protein was present in cultured erythrophores. Studies of cells induced to aggregate and disperse their pigment granules revealed that the 300 K protein comigrated with the pigment, suggesting that the 300 K protein may constitute part of the “α‐cytomatrix” involved in pigment translocations.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>MAP‐2 Involvement in Organelle Transport</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Evidence that MAP‐2 may be involved in pigment granule transport in squirrel fish erythrophores</title></titleInfo><name type="personal"><namePart type="given">Mark E.</namePart><namePart type="family">Stearns</namePart><affiliation>Department of Pharmacology, Fox Chase Cancer Center, Philadelphia</affiliation><description>Correspondence: Department of Pharmacology, Fox Chase Cancer Center, 7701 Burholme Ave., Philadelphia, PA 19111</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lester I.</namePart><namePart type="family">Binder</namePart><affiliation>Department of Anatomy, University of Alabama, Birmingham</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">1987</dateIssued><dateCaptured encoding="w3cdtf">1986-08-25</dateCaptured><dateValid encoding="w3cdtf">1986-11-17</dateValid><copyrightDate encoding="w3cdtf">1987</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">20</extent><extent unit="references">30</extent></physicalDescription><abstract lang="en">We have demonstrated the presence of MAP‐2 in squirrel fish erythrophores using SDS‐PAGE, immunobolt, and immunoprecipitation techniques. The monoclonal antibodies used (AP‐9, ‐13, ‐14) were raised against distinct antigenic sites on Chinese hamster brain MAP‐2. Immunoprecipitation studies demonstrated that all three antibodies bind a 300 K protein found in crude cell extracts and in partially purified MAP fractions isolated from erythrophores of the squirrel fish Holocentrus rufus. Immunofluorescent studies confirmed that the 300 K protein was present in cultured erythrophores. Studies of cells induced to aggregate and disperse their pigment granules revealed that the 300 K protein comigrated with the pigment, suggesting that the 300 K protein may constitute part of the “α‐cytomatrix” involved in pigment translocations.</abstract><subject lang="en"><genre>keywords</genre><topic>α‐cytomatrix</topic><topic>monoclonal antibodies</topic><topic>immnuolabeling</topic></subject><relatedItem type="host"><titleInfo><title>Cell Motility and the Cytoskeleton</title></titleInfo><titleInfo type="abbreviated"><title>Cell Motil. 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