LotaV3, PascalFrancis, Corpus, bibRecord, 000047

Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p'-DDT and its metabolite p,p'-DDE

Identifieur interne : 000047 ( PascalFrancis/Corpus ); précédent : 000046; suivant : 000048

Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p'-DDT and its metabolite p,p'-DDE

Auteurs : Ewa Gregoraszczuk ; Agnieszka Rak ; K. Kawalec ; Erik Ropstad

Source :

Toxicology letters [ 0378-4274 ] ; 2008.

RBID : Pascal:08-0273628

Descripteurs français

Pascal (Inist)
- DDT, Stéroïde, Sécrétion, DDE, Exposition, Ovaire, Cellule folliculaire, Mélange, Retardateur flamme, Métabolite, Follicule ovarien, Viabilité, Apoptose, Mort cellulaire, Congénère, Polybromobiphényle dérivé, Phényle éther(polybromo).

English descriptors

KwdEn :
- Apoptosis, Cell death, DDE, DDT, Exposure, Flame retardant, Follicular cell, Metabolite, Mixture, Ovarian follicle, Ovary, Secretion, Steroid, Viability.

Abstract

The main objective of the present study was to describe in vitro effects of individual congeners of polybrominateddibenzoethers (PBDEs) (PBDE-47, 99, 100, 209), p,p' -dichlorodiphenyltrichloroethane (DDT) and p,p' -dichlorodiphenyldichloroethylene (DDE) present at the highest amount in real-life mixtures of POPs extracted from liver oil of burbot from Lake Mjøsa in Norway, and defined combinations of these compounds on steroidogenesis in porcine ovarian follicular cells. Co-culture of theca and granulosa cells (Gc) was exposed by individual congeners of PBDEs or mixture. Media samples were collected and used for cell viability and steroid determination, while cells were used for the measurement of caspase-3 activity. All investigated PBDEs congeners increased both testosterone (T) and estradiol (E2) secretion. We noted additive stimulatory action of BDE-47 and BDE-100 on both T and E2 secretion, and then inhibitory action on both of these hormones production when additionally BDE-99 and then BDE-209 were included in this mixture. The situation was different when DDT and DDE were added to the defined mixture. Stimulatory action on E2 secretion with parallel decrease ofT secretion was noted. In conclusion, the data indicated: (1) stimulatory action of individual congeners of PBDEs on both E2 and T secretion by ovarian follicular cells points to stimulatory action on enzymes responsible for testosterone secretion; (2) the overall effect of a real-life mixture on the steroidogenic response in ovarian follicular cells could be dependent on the concentration of DDTs in the mixture; (3) the predicted effect of mixture, calculated based on a sum of independent action of individual congeners, was higher than that noted under the influence of defined mixture.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

A01	`01`	`1`		`@0 0378-4274`
A02	`01`			`@0 TOLED5`
A03		`1`		`@0 Toxicol. lett.`
A05				`@2 178`
A06				`@2 2`
A08	`01`	`1`	`ENG`	`@1 Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p'-DDT and its metabolite p,p'-DDE`
A11	`01`	`1`		`@1 GREGORASZCZUK (Ewa Ł.)`
A11	`02`	`1`		`@1 RAK (Agnieszka)`
A11	`03`	`1`		`@1 KAWALEC (K.)`
A11	`04`	`1`		`@1 ROPSTAD (Erik)`
A14	`01`			`@1 Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Ingardena 6 @2 30-060 Krakow @3 POL @Z 1 aut. @Z 2 aut. @Z 3 aut.`
A14	`02`			`@1 Norwegian School of Veterinary Science, Department of Production Animal Clinical Sciences @2 0033 Oslo @3 NOR @Z 4 aut.`
A20				`@1 103-109`
A21				`@1 2008`
A23	`01`			`@0 ENG`
A43	`01`			`@1 INIST @2 15984L @5 354000173784240060`
A44				`@0 0000 @1 © 2008 INIST-CNRS. All rights reserved.`
A45				`@0 3/4 p.`
A47	`01`	`1`		`@0 08-0273628`
A60				`@1 P`
A61				`@0 A`
A64	`01`	`1`		`@0 Toxicology letters`
A66	`01`			`@0 IRL`
C01	`01`		`ENG`	@0 The main objective of the present study was to describe in vitro effects of individual congeners of polybrominateddibenzoethers (PBDEs) (PBDE-47, 99, 100, 209), p,p' -dichlorodiphenyltrichloroethane (DDT) and p,p' -dichlorodiphenyldichloroethylene (DDE) present at the highest amount in real-life mixtures of POPs extracted from liver oil of burbot from Lake Mjøsa in Norway, and defined combinations of these compounds on steroidogenesis in porcine ovarian follicular cells. Co-culture of theca and granulosa cells (Gc) was exposed by individual congeners of PBDEs or mixture. Media samples were collected and used for cell viability and steroid determination, while cells were used for the measurement of caspase-3 activity. All investigated PBDEs congeners increased both testosterone (T) and estradiol (E2) secretion. We noted additive stimulatory action of BDE-47 and BDE-100 on both T and E2 secretion, and then inhibitory action on both of these hormones production when additionally BDE-99 and then BDE-209 were included in this mixture. The situation was different when DDT and DDE were added to the defined mixture. Stimulatory action on E2 secretion with parallel decrease ofT secretion was noted. In conclusion, the data indicated: (1) stimulatory action of individual congeners of PBDEs on both E2 and T secretion by ovarian follicular cells points to stimulatory action on enzymes responsible for testosterone secretion; (2) the overall effect of a real-life mixture on the steroidogenic response in ovarian follicular cells could be dependent on the concentration of DDTs in the mixture; (3) the predicted effect of mixture, calculated based on a sum of independent action of individual congeners, was higher than that noted under the influence of defined mixture.
C02	`01`	`X`		`@0 002B03G`
C03	`01`	`X`	`FRE`	`@0 DDT @2 NK @2 FF @2 FX @5 01`
C03	`01`	`X`	`ENG`	`@0 DDT @2 NK @2 FF @2 FX @5 01`
C03	`01`	`X`	`SPA`	`@0 DDT @2 NK @2 FF @2 FX @5 01`
C03	`02`	`X`	`FRE`	`@0 Stéroïde @5 02`
C03	`02`	`X`	`ENG`	`@0 Steroid @5 02`
C03	`02`	`X`	`SPA`	`@0 Esteroide @5 02`
C03	`03`	`X`	`FRE`	`@0 Sécrétion @5 03`
C03	`03`	`X`	`ENG`	`@0 Secretion @5 03`
C03	`03`	`X`	`SPA`	`@0 Secreción @5 03`
C03	`04`	`X`	`FRE`	`@0 DDE @2 NK @2 FF @5 04`
C03	`04`	`X`	`ENG`	`@0 DDE @2 NK @2 FF @5 04`
C03	`04`	`X`	`SPA`	`@0 DDE @2 NK @2 FF @5 04`
C03	`05`	`X`	`FRE`	`@0 Exposition @5 05`
C03	`05`	`X`	`ENG`	`@0 Exposure @5 05`
C03	`05`	`X`	`SPA`	`@0 Exposición @5 05`
C03	`06`	`X`	`FRE`	`@0 Ovaire @5 06`
C03	`06`	`X`	`ENG`	`@0 Ovary @5 06`
C03	`06`	`X`	`SPA`	`@0 Ovario @5 06`
C03	`07`	`X`	`FRE`	`@0 Cellule folliculaire @5 08`
C03	`07`	`X`	`ENG`	`@0 Follicular cell @5 08`
C03	`07`	`X`	`SPA`	`@0 Célula folicular @5 08`
C03	`08`	`X`	`FRE`	`@0 Mélange @5 09`
C03	`08`	`X`	`ENG`	`@0 Mixture @5 09`
C03	`08`	`X`	`SPA`	`@0 Mezcla @5 09`
C03	`09`	`X`	`FRE`	`@0 Retardateur flamme @5 11`
C03	`09`	`X`	`ENG`	`@0 Flame retardant @5 11`
C03	`09`	`X`	`SPA`	`@0 Retardador llama @5 11`
C03	`10`	`X`	`FRE`	`@0 Métabolite @5 12`
C03	`10`	`X`	`ENG`	`@0 Metabolite @5 12`
C03	`10`	`X`	`SPA`	`@0 Metabolito @5 12`
C03	`11`	`X`	`FRE`	`@0 Follicule ovarien @5 16`
C03	`11`	`X`	`ENG`	`@0 Ovarian follicle @5 16`
C03	`11`	`X`	`SPA`	`@0 Folículo ovárico @5 16`
C03	`12`	`X`	`FRE`	`@0 Viabilité @5 17`
C03	`12`	`X`	`ENG`	`@0 Viability @5 17`
C03	`12`	`X`	`SPA`	`@0 Viabilidad @5 17`
C03	`13`	`X`	`FRE`	`@0 Apoptose @5 18`
C03	`13`	`X`	`ENG`	`@0 Apoptosis @5 18`
C03	`13`	`X`	`SPA`	`@0 Apoptosis @5 18`
C03	`14`	`X`	`FRE`	`@0 Mort cellulaire @5 19`
C03	`14`	`X`	`ENG`	`@0 Cell death @5 19`
C03	`14`	`X`	`SPA`	`@0 Muerte celular @5 19`
C03	`15`	`X`	`FRE`	`@0 Congénère @4 INC @5 86`
C03	`16`	`X`	`FRE`	`@0 Polybromobiphényle dérivé @4 INC @5 87`
C03	`17`	`X`	`FRE`	`@0 Phényle éther(polybromo) @4 INC @5 88`
C07	`01`	`X`	`FRE`	`@0 Appareil génital femelle @5 61`
C07	`01`	`X`	`ENG`	`@0 Female genital system @5 61`
C07	`01`	`X`	`SPA`	`@0 Aparato genital hembra @5 61`
C07	`02`	`X`	`FRE`	`@0 Brome Composé organique @2 NC @2 FR @2 FX @2 NA @5 62`
C07	`02`	`X`	`ENG`	`@0 Bromine Organic compounds @2 NC @2 FR @2 FX @2 NA @5 62`
C07	`02`	`X`	`SPA`	`@0 Bromo Compuesto orgánico @2 NC @2 FR @2 FX @2 NA @5 62`
C07	`03`	`X`	`FRE`	`@0 Insecticide @5 63`
C07	`03`	`X`	`ENG`	`@0 Insecticide @5 63`
C07	`03`	`X`	`SPA`	`@0 Insecticida @5 63`
C07	`04`	`X`	`FRE`	`@0 Pesticide`
C07	`04`	`X`	`ENG`	`@0 Pesticides`
C07	`04`	`X`	`SPA`	`@0 Plaguicida`
C07	`05`	`X`	`FRE`	`@0 Organochloré @5 64`
C07	`05`	`X`	`ENG`	`@0 Organochlorine compounds @5 64`
C07	`05`	`X`	`SPA`	`@0 Organoclorado @5 64`
N21				`@1 175`
N44	`01`			`@1 OTO`
N82				`@1 OTO`

Format Inist (serveur)

NO :	PASCAL 08-0273628 INIST
ET :	Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p'-DDT and its metabolite p,p'-DDE
AU :	GREGORASZCZUK (Ewa Ł.); RAK (Agnieszka); KAWALEC (K.); ROPSTAD (Erik)
AF :	Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Ingardena 6/30-060 Krakow/Pologne (1 aut., 2 aut., 3 aut.); Norwegian School of Veterinary Science, Department of Production Animal Clinical Sciences/0033 Oslo/Norvège (4 aut.)
DT :	Publication en série; Niveau analytique
SO :	Toxicology letters; ISSN 0378-4274; Coden TOLED5; Irlande; Da. 2008; Vol. 178; No. 2; Pp. 103-109; Bibl. 3/4 p.
LA :	Anglais
EA :	The main objective of the present study was to describe in vitro effects of individual congeners of polybrominateddibenzoethers (PBDEs) (PBDE-47, 99, 100, 209), p,p' -dichlorodiphenyltrichloroethane (DDT) and p,p' -dichlorodiphenyldichloroethylene (DDE) present at the highest amount in real-life mixtures of POPs extracted from liver oil of burbot from Lake Mjøsa in Norway, and defined combinations of these compounds on steroidogenesis in porcine ovarian follicular cells. Co-culture of theca and granulosa cells (Gc) was exposed by individual congeners of PBDEs or mixture. Media samples were collected and used for cell viability and steroid determination, while cells were used for the measurement of caspase-3 activity. All investigated PBDEs congeners increased both testosterone (T) and estradiol (E2) secretion. We noted additive stimulatory action of BDE-47 and BDE-100 on both T and E2 secretion, and then inhibitory action on both of these hormones production when additionally BDE-99 and then BDE-209 were included in this mixture. The situation was different when DDT and DDE were added to the defined mixture. Stimulatory action on E2 secretion with parallel decrease ofT secretion was noted. In conclusion, the data indicated: (1) stimulatory action of individual congeners of PBDEs on both E2 and T secretion by ovarian follicular cells points to stimulatory action on enzymes responsible for testosterone secretion; (2) the overall effect of a real-life mixture on the steroidogenic response in ovarian follicular cells could be dependent on the concentration of DDTs in the mixture; (3) the predicted effect of mixture, calculated based on a sum of independent action of individual congeners, was higher than that noted under the influence of defined mixture.
CC :	002B03G
FD :	DDT; Stéroïde; Sécrétion; DDE; Exposition; Ovaire; Cellule folliculaire; Mélange; Retardateur flamme; Métabolite; Follicule ovarien; Viabilité; Apoptose; Mort cellulaire; Congénère; Polybromobiphényle dérivé; Phényle éther(polybromo)
FG :	Appareil génital femelle; Brome Composé organique; Insecticide; Pesticide; Organochloré
ED :	DDT; Steroid; Secretion; DDE; Exposure; Ovary; Follicular cell; Mixture; Flame retardant; Metabolite; Ovarian follicle; Viability; Apoptosis; Cell death
EG :	Female genital system; Bromine Organic compounds; Insecticide; Pesticides; Organochlorine compounds
SD :	DDT; Esteroide; Secreción; DDE; Exposición; Ovario; Célula folicular; Mezcla; Retardador llama; Metabolito; Folículo ovárico; Viabilidad; Apoptosis; Muerte celular
LO :	INIST-15984L.354000173784240060
ID :	08-0273628

Links to Exploration step

Pascal:08-0273628

Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apoptosis</term>
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<term>DDE</term>
<term>DDT</term>
<term>Exposure</term>
<term>Flame retardant</term>
<term>Follicular cell</term>
<term>Metabolite</term>
<term>Mixture</term>
<term>Ovarian follicle</term>
<term>Ovary</term>
<term>Secretion</term>
<term>Steroid</term>
<term>Viability</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>DDT</term>
<term>Stéroïde</term>
<term>Sécrétion</term>
<term>DDE</term>
<term>Exposition</term>
<term>Ovaire</term>
<term>Cellule folliculaire</term>
<term>Mélange</term>
<term>Retardateur flamme</term>
<term>Métabolite</term>
<term>Follicule ovarien</term>
<term>Viabilité</term>
<term>Apoptose</term>
<term>Mort cellulaire</term>
<term>Congénère</term>
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<front><div type="abstract" xml:lang="en">The main objective of the present study was to describe in vitro effects of individual congeners of polybrominateddibenzoethers (PBDEs) (PBDE-47, 99, 100, 209), p,p' -dichlorodiphenyltrichloroethane (DDT) and p,p' -dichlorodiphenyldichloroethylene (DDE) present at the highest amount in real-life mixtures of POPs extracted from liver oil of burbot from Lake Mjøsa in Norway, and defined combinations of these compounds on steroidogenesis in porcine ovarian follicular cells. Co-culture of theca and granulosa cells (Gc) was exposed by individual congeners of PBDEs or mixture. Media samples were collected and used for cell viability and steroid determination, while cells were used for the measurement of caspase-3 activity. All investigated PBDEs congeners increased both testosterone (T) and estradiol (E2) secretion. We noted additive stimulatory action of BDE-47 and BDE-100 on both T and E2 secretion, and then inhibitory action on both of these hormones production when additionally BDE-99 and then BDE-209 were included in this mixture. The situation was different when DDT and DDE were added to the defined mixture. Stimulatory action on E2 secretion with parallel decrease ofT secretion was noted. In conclusion, the data indicated: (1) stimulatory action of individual congeners of PBDEs on both E2 and T secretion by ovarian follicular cells points to stimulatory action on enzymes responsible for testosterone secretion; (2) the overall effect of a real-life mixture on the steroidogenic response in ovarian follicular cells could be dependent on the concentration of DDTs in the mixture; (3) the predicted effect of mixture, calculated based on a sum of independent action of individual congeners, was higher than that noted under the influence of defined mixture.</div>
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<fA11 i1="01" i2="1"><s1>GREGORASZCZUK (Ewa Ł.)</s1>
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<fA14 i1="02"><s1>Norwegian School of Veterinary Science, Department of Production Animal Clinical Sciences</s1>
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<fA44><s0>0000</s0>
<s1>© 2008 INIST-CNRS. All rights reserved.</s1>
</fA44>
<fA45><s0>3/4 p.</s0>
</fA45>
<fA47 i1="01" i2="1"><s0>08-0273628</s0>
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<fA60><s1>P</s1>
</fA60>
<fA61><s0>A</s0>
</fA61>
<fA64 i1="01" i2="1"><s0>Toxicology letters</s0>
</fA64>
<fA66 i1="01"><s0>IRL</s0>
</fA66>
<fC01 i1="01" l="ENG"><s0>The main objective of the present study was to describe in vitro effects of individual congeners of polybrominateddibenzoethers (PBDEs) (PBDE-47, 99, 100, 209), p,p' -dichlorodiphenyltrichloroethane (DDT) and p,p' -dichlorodiphenyldichloroethylene (DDE) present at the highest amount in real-life mixtures of POPs extracted from liver oil of burbot from Lake Mjøsa in Norway, and defined combinations of these compounds on steroidogenesis in porcine ovarian follicular cells. Co-culture of theca and granulosa cells (Gc) was exposed by individual congeners of PBDEs or mixture. Media samples were collected and used for cell viability and steroid determination, while cells were used for the measurement of caspase-3 activity. All investigated PBDEs congeners increased both testosterone (T) and estradiol (E2) secretion. We noted additive stimulatory action of BDE-47 and BDE-100 on both T and E2 secretion, and then inhibitory action on both of these hormones production when additionally BDE-99 and then BDE-209 were included in this mixture. The situation was different when DDT and DDE were added to the defined mixture. Stimulatory action on E2 secretion with parallel decrease ofT secretion was noted. In conclusion, the data indicated: (1) stimulatory action of individual congeners of PBDEs on both E2 and T secretion by ovarian follicular cells points to stimulatory action on enzymes responsible for testosterone secretion; (2) the overall effect of a real-life mixture on the steroidogenic response in ovarian follicular cells could be dependent on the concentration of DDTs in the mixture; (3) the predicted effect of mixture, calculated based on a sum of independent action of individual congeners, was higher than that noted under the influence of defined mixture.</s0>
</fC01>
<fC02 i1="01" i2="X"><s0>002B03G</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE"><s0>DDT</s0>
<s2>NK</s2>
<s2>FF</s2>
<s2>FX</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG"><s0>DDT</s0>
<s2>NK</s2>
<s2>FF</s2>
<s2>FX</s2>
<s5>01</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA"><s0>DDT</s0>
<s2>NK</s2>
<s2>FF</s2>
<s2>FX</s2>
<s5>01</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE"><s0>Stéroïde</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG"><s0>Steroid</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA"><s0>Esteroide</s0>
<s5>02</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE"><s0>Sécrétion</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG"><s0>Secretion</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA"><s0>Secreción</s0>
<s5>03</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE"><s0>DDE</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG"><s0>DDE</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA"><s0>DDE</s0>
<s2>NK</s2>
<s2>FF</s2>
<s5>04</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE"><s0>Exposition</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG"><s0>Exposure</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA"><s0>Exposición</s0>
<s5>05</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE"><s0>Ovaire</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG"><s0>Ovary</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA"><s0>Ovario</s0>
<s5>06</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE"><s0>Cellule folliculaire</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG"><s0>Follicular cell</s0>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA"><s0>Célula folicular</s0>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE"><s0>Mélange</s0>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG"><s0>Mixture</s0>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA"><s0>Mezcla</s0>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE"><s0>Retardateur flamme</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG"><s0>Flame retardant</s0>
<s5>11</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA"><s0>Retardador llama</s0>
<s5>11</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE"><s0>Métabolite</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG"><s0>Metabolite</s0>
<s5>12</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA"><s0>Metabolito</s0>
<s5>12</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE"><s0>Follicule ovarien</s0>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG"><s0>Ovarian follicle</s0>
<s5>16</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA"><s0>Folículo ovárico</s0>
<s5>16</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE"><s0>Viabilité</s0>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG"><s0>Viability</s0>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA"><s0>Viabilidad</s0>
<s5>17</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE"><s0>Apoptose</s0>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG"><s0>Apoptosis</s0>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA"><s0>Apoptosis</s0>
<s5>18</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE"><s0>Mort cellulaire</s0>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG"><s0>Cell death</s0>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA"><s0>Muerte celular</s0>
<s5>19</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE"><s0>Congénère</s0>
<s4>INC</s4>
<s5>86</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE"><s0>Polybromobiphényle dérivé</s0>
<s4>INC</s4>
<s5>87</s5>
</fC03>
<fC03 i1="17" i2="X" l="FRE"><s0>Phényle éther(polybromo)</s0>
<s4>INC</s4>
<s5>88</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE"><s0>Appareil génital femelle</s0>
<s5>61</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG"><s0>Female genital system</s0>
<s5>61</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA"><s0>Aparato genital hembra</s0>
<s5>61</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE"><s0>Brome Composé organique</s0>
<s2>NC</s2>
<s2>FR</s2>
<s2>FX</s2>
<s2>NA</s2>
<s5>62</s5>
</fC07>
<fC07 i1="02" i2="X" l="ENG"><s0>Bromine Organic compounds</s0>
<s2>NC</s2>
<s2>FR</s2>
<s2>FX</s2>
<s2>NA</s2>
<s5>62</s5>
</fC07>
<fC07 i1="02" i2="X" l="SPA"><s0>Bromo Compuesto orgánico</s0>
<s2>NC</s2>
<s2>FR</s2>
<s2>FX</s2>
<s2>NA</s2>
<s5>62</s5>
</fC07>
<fC07 i1="03" i2="X" l="FRE"><s0>Insecticide</s0>
<s5>63</s5>
</fC07>
<fC07 i1="03" i2="X" l="ENG"><s0>Insecticide</s0>
<s5>63</s5>
</fC07>
<fC07 i1="03" i2="X" l="SPA"><s0>Insecticida</s0>
<s5>63</s5>
</fC07>
<fC07 i1="04" i2="X" l="FRE"><s0>Pesticide</s0>
</fC07>
<fC07 i1="04" i2="X" l="ENG"><s0>Pesticides</s0>
</fC07>
<fC07 i1="04" i2="X" l="SPA"><s0>Plaguicida</s0>
</fC07>
<fC07 i1="05" i2="X" l="FRE"><s0>Organochloré</s0>
<s5>64</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG"><s0>Organochlorine compounds</s0>
<s5>64</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA"><s0>Organoclorado</s0>
<s5>64</s5>
</fC07>
<fN21><s1>175</s1>
</fN21>
<fN44 i1="01"><s1>OTO</s1>
</fN44>
<fN82><s1>OTO</s1>
</fN82>
</pA>
</standard>
<server><NO>PASCAL 08-0273628 INIST</NO>
<ET>Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p'-DDT and its metabolite p,p'-DDE</ET>
<AU>GREGORASZCZUK (Ewa Ł.); RAK (Agnieszka); KAWALEC (K.); ROPSTAD (Erik)</AU>
<AF>Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Ingardena 6/30-060 Krakow/Pologne (1 aut., 2 aut., 3 aut.); Norwegian School of Veterinary Science, Department of Production Animal Clinical Sciences/0033 Oslo/Norvège (4 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Toxicology letters; ISSN 0378-4274; Coden TOLED5; Irlande; Da. 2008; Vol. 178; No. 2; Pp. 103-109; Bibl. 3/4 p.</SO>
<LA>Anglais</LA>
<EA>The main objective of the present study was to describe in vitro effects of individual congeners of polybrominateddibenzoethers (PBDEs) (PBDE-47, 99, 100, 209), p,p' -dichlorodiphenyltrichloroethane (DDT) and p,p' -dichlorodiphenyldichloroethylene (DDE) present at the highest amount in real-life mixtures of POPs extracted from liver oil of burbot from Lake Mjøsa in Norway, and defined combinations of these compounds on steroidogenesis in porcine ovarian follicular cells. Co-culture of theca and granulosa cells (Gc) was exposed by individual congeners of PBDEs or mixture. Media samples were collected and used for cell viability and steroid determination, while cells were used for the measurement of caspase-3 activity. All investigated PBDEs congeners increased both testosterone (T) and estradiol (E2) secretion. We noted additive stimulatory action of BDE-47 and BDE-100 on both T and E2 secretion, and then inhibitory action on both of these hormones production when additionally BDE-99 and then BDE-209 were included in this mixture. The situation was different when DDT and DDE were added to the defined mixture. Stimulatory action on E2 secretion with parallel decrease ofT secretion was noted. In conclusion, the data indicated: (1) stimulatory action of individual congeners of PBDEs on both E2 and T secretion by ovarian follicular cells points to stimulatory action on enzymes responsible for testosterone secretion; (2) the overall effect of a real-life mixture on the steroidogenic response in ovarian follicular cells could be dependent on the concentration of DDTs in the mixture; (3) the predicted effect of mixture, calculated based on a sum of independent action of individual congeners, was higher than that noted under the influence of defined mixture.</EA>
<CC>002B03G</CC>
<FD>DDT; Stéroïde; Sécrétion; DDE; Exposition; Ovaire; Cellule folliculaire; Mélange; Retardateur flamme; Métabolite; Follicule ovarien; Viabilité; Apoptose; Mort cellulaire; Congénère; Polybromobiphényle dérivé; Phényle éther(polybromo)</FD>
<FG>Appareil génital femelle; Brome Composé organique; Insecticide; Pesticide; Organochloré</FG>
<ED>DDT; Steroid; Secretion; DDE; Exposure; Ovary; Follicular cell; Mixture; Flame retardant; Metabolite; Ovarian follicle; Viability; Apoptosis; Cell death</ED>
<EG>Female genital system; Bromine Organic compounds; Insecticide; Pesticides; Organochlorine compounds</EG>
<SD>DDT; Esteroide; Secreción; DDE; Exposición; Ovario; Célula folicular; Mezcla; Retardador llama; Metabolito; Folículo ovárico; Viabilidad; Apoptosis; Muerte celular</SD>
<LO>INIST-15984L.354000173784240060</LO>
<ID>08-0273628</ID>
</server>
</inist>
</record>

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Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p'-DDT and its metabolite p,p'-DDE

Steroid secretion following exposure of ovarian follicular cells to single congeners and defined mixture of polybrominateddibenzoethers (PBDEs), p,p'-DDT and its metabolite p,p'-DDE

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