Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs).
Identifieur interne : 000658 ( Main/Merge ); précédent : 000657; suivant : 000659Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs).
Auteurs : Ewa L. Gregoraszczuk [Pologne] ; Katarzyna Milczarek ; Anna K. W Jtowicz ; Vidar Berg ; Janneche U. Skaare ; Erik RopstadSource :
- Reproductive toxicology (Elmsford, N.Y.) [ 0890-6238 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Apoptose (effets des médicaments et des substances chimiques), Appréciation des risques (MeSH), Caspase-3 (métabolisme), Cellules cultivées (MeSH), Cellules de la granulosa (effets des médicaments et des substances chimiques), Cellules de la granulosa (métabolisme), Cellules thécales (effets des médicaments et des substances chimiques), Cellules thécales (métabolisme), Facteurs temps (MeSH), Femelle (MeSH), Gadiformes (MeSH), Gadus morhua (MeSH), Hormones sexuelles stéroïdiennes (métabolisme), Huile de foie de morue (composition chimique), Huile de foie de morue (toxicité), L-Lactate dehydrogenase (métabolisme), Norvège (MeSH), Oestradiol (métabolisme), Perturbateurs endocriniens (composition chimique), Perturbateurs endocriniens (isolement et purification), Polluants chimiques de l'eau (isolement et purification), Polluants chimiques de l'eau (toxicité), Progestérone (métabolisme), Protéines de poisson (métabolisme), Relation dose-effet des médicaments (MeSH), Suidae (MeSH), Survie cellulaire (effets des médicaments et des substances chimiques), Techniques de coculture (MeSH).
- MESH :
- composition chimique : Huile de foie de morue, Perturbateurs endocriniens.
- effets des médicaments et des substances chimiques : Apoptose, Cellules de la granulosa, Cellules thécales, Survie cellulaire.
- isolement et purification : Perturbateurs endocriniens, Polluants chimiques de l'eau.
- métabolisme : Caspase-3, Cellules de la granulosa, Cellules thécales, Hormones sexuelles stéroïdiennes, L-Lactate dehydrogenase, Oestradiol, Progestérone, Protéines de poisson.
- toxicité : Huile de foie de morue, Polluants chimiques de l'eau.
- Animaux, Appréciation des risques, Cellules cultivées, Facteurs temps, Femelle, Gadiformes, Gadus morhua, Norvège, Relation dose-effet des médicaments, Suidae, Techniques de coculture.
- Wicri :
- geographic : Norvège.
English descriptors
- KwdEn :
- Animals (MeSH), Apoptosis (drug effects), Caspase 3 (metabolism), Cell Survival (drug effects), Cells, Cultured (MeSH), Coculture Techniques (MeSH), Cod Liver Oil (chemistry), Cod Liver Oil (toxicity), Dose-Response Relationship, Drug (MeSH), Endocrine Disruptors (chemistry), Endocrine Disruptors (isolation & purification), Estradiol (metabolism), Female (MeSH), Fish Proteins (metabolism), Gadiformes (MeSH), Gadus morhua (MeSH), Gonadal Steroid Hormones (metabolism), Granulosa Cells (drug effects), Granulosa Cells (metabolism), L-Lactate Dehydrogenase (metabolism), Norway (MeSH), Progesterone (metabolism), Risk Assessment (MeSH), Swine (MeSH), Theca Cells (drug effects), Theca Cells (metabolism), Time Factors (MeSH), Water Pollutants, Chemical (isolation & purification), Water Pollutants, Chemical (toxicity).
- MESH :
- chemical , chemistry : Cod Liver Oil, Endocrine Disruptors.
- chemical , isolation & purification : Endocrine Disruptors, Water Pollutants, Chemical.
- chemical , metabolism : Caspase 3, Estradiol, Fish Proteins, Gonadal Steroid Hormones, L-Lactate Dehydrogenase, Progesterone.
- chemical , toxicity : Water Pollutants, Chemical.
- geographic : Norway.
- drug effects : Apoptosis, Cell Survival, Granulosa Cells, Theca Cells.
- metabolism : Granulosa Cells, Theca Cells.
- chemical , toxicity : Cod Liver Oil.
- Animals, Cells, Cultured, Coculture Techniques, Dose-Response Relationship, Drug, Female, Gadiformes, Gadus morhua, Risk Assessment, Swine, Time Factors.
Abstract
This study investigated in vitro endocrine disrupting effects of three mixtures of POPs: 'Marine mix' extracted from Atlantic cod liver, and two mixtures extracted from burbot liver, 'Mjøsa mix' and 'Losna mix'. The POP mixtures were chemically characterized. Co-culture of theca and granulosa cells, were exposed for 48h with different doses of 'Marine mix', 'Mjøsa mix' or 'Losna mix'. As an end point cell viability was determinated by LDH test, steroid analysis by EIA and caspase-3 by colorimetric substrate. Chemical characterization of the mixtures demonstrated that the 'Marine mix' contained high levels of DDTs and PCBs. In the 'Mjøsa mix', the dominant pollutants were BDEs and HBCD. The concentrations of POPs measured in the 'Losna mix' were considerably lower. All mixtures used in the present study had a stimulatory effect on testosterone and estradiol secretion with 'Marine mix'>'Mjøsa mix'>'Losna mix'. These results show that even a mixture containing background concentrations of POPs significantly affected steroid secretion. A higher steroidogenic response in low dose ranges, compared with high dose ranges indicated xenobiotic-conditioning hormesis. This could complicate predictions of effects in risk assessments.
DOI: 10.1016/j.reprotox.2007.10.001
PubMed: 18024081
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<author><name sortKey="Gregoraszczuk, Ewa L" sort="Gregoraszczuk, Ewa L" uniqKey="Gregoraszczuk E" first="Ewa L" last="Gregoraszczuk">Ewa L. Gregoraszczuk</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków, Poland. greg@zuk.iz.uj.edu.pl</nlm:affiliation>
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<wicri:regionArea>Department of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków</wicri:regionArea>
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<author><name sortKey="Milczarek, Katarzyna" sort="Milczarek, Katarzyna" uniqKey="Milczarek K" first="Katarzyna" last="Milczarek">Katarzyna Milczarek</name>
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<author><name sortKey="W Jtowicz, Anna K" sort="W Jtowicz, Anna K" uniqKey="W Jtowicz A" first="Anna K" last="W Jtowicz">Anna K. W Jtowicz</name>
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<author><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name>
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<author><name sortKey="Skaare, Janneche U" sort="Skaare, Janneche U" uniqKey="Skaare J" first="Janneche U" last="Skaare">Janneche U. Skaare</name>
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<author><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Steroid secretion following exposure of ovarian follicular cells to three different natural mixtures of persistent organic pollutants (POPs).</title>
<author><name sortKey="Gregoraszczuk, Ewa L" sort="Gregoraszczuk, Ewa L" uniqKey="Gregoraszczuk E" first="Ewa L" last="Gregoraszczuk">Ewa L. Gregoraszczuk</name>
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<author><name sortKey="Milczarek, Katarzyna" sort="Milczarek, Katarzyna" uniqKey="Milczarek K" first="Katarzyna" last="Milczarek">Katarzyna Milczarek</name>
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<author><name sortKey="Berg, Vidar" sort="Berg, Vidar" uniqKey="Berg V" first="Vidar" last="Berg">Vidar Berg</name>
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<author><name sortKey="Skaare, Janneche U" sort="Skaare, Janneche U" uniqKey="Skaare J" first="Janneche U" last="Skaare">Janneche U. Skaare</name>
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<author><name sortKey="Ropstad, Erik" sort="Ropstad, Erik" uniqKey="Ropstad E" first="Erik" last="Ropstad">Erik Ropstad</name>
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<series><title level="j">Reproductive toxicology (Elmsford, N.Y.)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Apoptosis (drug effects)</term>
<term>Caspase 3 (metabolism)</term>
<term>Cell Survival (drug effects)</term>
<term>Cells, Cultured (MeSH)</term>
<term>Coculture Techniques (MeSH)</term>
<term>Cod Liver Oil (chemistry)</term>
<term>Cod Liver Oil (toxicity)</term>
<term>Dose-Response Relationship, Drug (MeSH)</term>
<term>Endocrine Disruptors (chemistry)</term>
<term>Endocrine Disruptors (isolation & purification)</term>
<term>Estradiol (metabolism)</term>
<term>Female (MeSH)</term>
<term>Fish Proteins (metabolism)</term>
<term>Gadiformes (MeSH)</term>
<term>Gadus morhua (MeSH)</term>
<term>Gonadal Steroid Hormones (metabolism)</term>
<term>Granulosa Cells (drug effects)</term>
<term>Granulosa Cells (metabolism)</term>
<term>L-Lactate Dehydrogenase (metabolism)</term>
<term>Norway (MeSH)</term>
<term>Progesterone (metabolism)</term>
<term>Risk Assessment (MeSH)</term>
<term>Swine (MeSH)</term>
<term>Theca Cells (drug effects)</term>
<term>Theca Cells (metabolism)</term>
<term>Time Factors (MeSH)</term>
<term>Water Pollutants, Chemical (isolation & purification)</term>
<term>Water Pollutants, Chemical (toxicity)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Apoptose (effets des médicaments et des substances chimiques)</term>
<term>Appréciation des risques (MeSH)</term>
<term>Caspase-3 (métabolisme)</term>
<term>Cellules cultivées (MeSH)</term>
<term>Cellules de la granulosa (effets des médicaments et des substances chimiques)</term>
<term>Cellules de la granulosa (métabolisme)</term>
<term>Cellules thécales (effets des médicaments et des substances chimiques)</term>
<term>Cellules thécales (métabolisme)</term>
<term>Facteurs temps (MeSH)</term>
<term>Femelle (MeSH)</term>
<term>Gadiformes (MeSH)</term>
<term>Gadus morhua (MeSH)</term>
<term>Hormones sexuelles stéroïdiennes (métabolisme)</term>
<term>Huile de foie de morue (composition chimique)</term>
<term>Huile de foie de morue (toxicité)</term>
<term>L-Lactate dehydrogenase (métabolisme)</term>
<term>Norvège (MeSH)</term>
<term>Oestradiol (métabolisme)</term>
<term>Perturbateurs endocriniens (composition chimique)</term>
<term>Perturbateurs endocriniens (isolement et purification)</term>
<term>Polluants chimiques de l'eau (isolement et purification)</term>
<term>Polluants chimiques de l'eau (toxicité)</term>
<term>Progestérone (métabolisme)</term>
<term>Protéines de poisson (métabolisme)</term>
<term>Relation dose-effet des médicaments (MeSH)</term>
<term>Suidae (MeSH)</term>
<term>Survie cellulaire (effets des médicaments et des substances chimiques)</term>
<term>Techniques de coculture (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cod Liver Oil</term>
<term>Endocrine Disruptors</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Endocrine Disruptors</term>
<term>Water Pollutants, Chemical</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Caspase 3</term>
<term>Estradiol</term>
<term>Fish Proteins</term>
<term>Gonadal Steroid Hormones</term>
<term>L-Lactate Dehydrogenase</term>
<term>Progesterone</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Water Pollutants, Chemical</term>
</keywords>
<keywords scheme="MESH" type="geographic" xml:lang="en"><term>Norway</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Huile de foie de morue</term>
<term>Perturbateurs endocriniens</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term>
<term>Cell Survival</term>
<term>Granulosa Cells</term>
<term>Theca Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Apoptose</term>
<term>Cellules de la granulosa</term>
<term>Cellules thécales</term>
<term>Survie cellulaire</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Perturbateurs endocriniens</term>
<term>Polluants chimiques de l'eau</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Granulosa Cells</term>
<term>Theca Cells</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Caspase-3</term>
<term>Cellules de la granulosa</term>
<term>Cellules thécales</term>
<term>Hormones sexuelles stéroïdiennes</term>
<term>L-Lactate dehydrogenase</term>
<term>Oestradiol</term>
<term>Progestérone</term>
<term>Protéines de poisson</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cod Liver Oil</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Huile de foie de morue</term>
<term>Polluants chimiques de l'eau</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cells, Cultured</term>
<term>Coculture Techniques</term>
<term>Dose-Response Relationship, Drug</term>
<term>Female</term>
<term>Gadiformes</term>
<term>Gadus morhua</term>
<term>Risk Assessment</term>
<term>Swine</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Appréciation des risques</term>
<term>Cellules cultivées</term>
<term>Facteurs temps</term>
<term>Femelle</term>
<term>Gadiformes</term>
<term>Gadus morhua</term>
<term>Norvège</term>
<term>Relation dose-effet des médicaments</term>
<term>Suidae</term>
<term>Techniques de coculture</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Norvège</term>
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<front><div type="abstract" xml:lang="en">This study investigated in vitro endocrine disrupting effects of three mixtures of POPs: 'Marine mix' extracted from Atlantic cod liver, and two mixtures extracted from burbot liver, 'Mjøsa mix' and 'Losna mix'. The POP mixtures were chemically characterized. Co-culture of theca and granulosa cells, were exposed for 48h with different doses of 'Marine mix', 'Mjøsa mix' or 'Losna mix'. As an end point cell viability was determinated by LDH test, steroid analysis by EIA and caspase-3 by colorimetric substrate. Chemical characterization of the mixtures demonstrated that the 'Marine mix' contained high levels of DDTs and PCBs. In the 'Mjøsa mix', the dominant pollutants were BDEs and HBCD. The concentrations of POPs measured in the 'Losna mix' were considerably lower. All mixtures used in the present study had a stimulatory effect on testosterone and estradiol secretion with 'Marine mix'>'Mjøsa mix'>'Losna mix'. These results show that even a mixture containing background concentrations of POPs significantly affected steroid secretion. A higher steroidogenic response in low dose ranges, compared with high dose ranges indicated xenobiotic-conditioning hormesis. This could complicate predictions of effects in risk assessments.</div>
</front>
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