Calcium‐induced quiescence of sperm motility in the bluegill (Lepomis macrochirus)
Identifieur interne : 000805 ( Main/Exploration ); précédent : 000804; suivant : 000806Calcium‐induced quiescence of sperm motility in the bluegill (Lepomis macrochirus)
Auteurs : Micah D. Zuccarelli [États-Unis] ; Rolf L. Ingermann [États-Unis]Source :
- Journal of Experimental Zoology Part A: Ecological Genetics and Physiology [ 1932-5223 ] ; 2007-10-01.
Abstract
Before dilution in hypoosmotic media sperm of freshwater fish are maintained quiescent by a range of factors including osmolality, K+ and pH, and the onset of motility is generally associated with an increase in cytoplasmic Ca2+. In contrast, Ca2+ in conjunction with osmolality was found to inhibit motility of intact bluegill sperm. Consistent with seminal plasma composition, 0.16 mmol/L Ca2+ and greater, in conjunction with an osmotic concentration of 290 mOsm/kg, inhibited the onset of bluegill sperm motility; sperm diluted in saline at 290 mOsm/kg without Ca2+ became motile. Cations Mn2+ and Sr2+, in conjunction with osmolality, had an inhibitory effect on initiation of sperm motility similar to that of Ca2+. Sperm motility was inhibited by Ca2+ channel blockers nimodipine and nifedipine, the mitochondrial Ca2+ uniporter inhibitor ruthenium red and the calmodulin inhibitors W‐7 and trifluoperazine dihydrochloride. These results provide evidence that elevated cytoplasmic Ca2+ inhibits sperm motility and yet low levels permit or promote motility. This study demonstrates a unique inhibitory action of Ca2+ on the motility of intact fish sperm at physiologically relevant levels. J. Exp. Zool. 307A:590–599, 2007. © 2007 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jez.414
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000194
- to stream Istex, to step Curation: 000194
- to stream Istex, to step Checkpoint: 000475
- to stream Main, to step Merge: 000817
- to stream Main, to step Curation: 000805
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Calcium‐induced quiescence of sperm motility in the bluegill (Lepomis macrochirus)</title><author><name sortKey="Zuccarelli, Micah D" sort="Zuccarelli, Micah D" uniqKey="Zuccarelli M" first="Micah D." last="Zuccarelli">Micah D. Zuccarelli</name></author><author><name sortKey="Ingermann, Rolf L" sort="Ingermann, Rolf L" uniqKey="Ingermann R" first="Rolf L." last="Ingermann">Rolf L. Ingermann</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:66309AB3948954F7282E5492A7364529B57887A6</idno><date when="2007" year="2007">2007</date><idno type="doi">10.1002/jez.414</idno><idno type="url">https://api.istex.fr/document/66309AB3948954F7282E5492A7364529B57887A6/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000194</idno><idno type="wicri:Area/Istex/Curation">000194</idno><idno type="wicri:Area/Istex/Checkpoint">000475</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000475</idno><idno type="wicri:doubleKey">1932-5223:2007:Zuccarelli M:calcium:induced:quiescence</idno><idno type="wicri:Area/Main/Merge">000817</idno><idno type="wicri:Area/Main/Curation">000805</idno><idno type="wicri:Area/Main/Exploration">000805</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Calcium‐induced quiescence of sperm motility in the bluegill (Lepomis macrochirus)</title><author><name sortKey="Zuccarelli, Micah D" sort="Zuccarelli, Micah D" uniqKey="Zuccarelli M" first="Micah D." last="Zuccarelli">Micah D. Zuccarelli</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Idaho</region></placeName><wicri:cityArea>Department of Biological Sciences, Center for Reproductive Biology, University of Idaho, Moscow</wicri:cityArea></affiliation></author><author><name sortKey="Ingermann, Rolf L" sort="Ingermann, Rolf L" uniqKey="Ingermann R" first="Rolf L." last="Ingermann">Rolf L. Ingermann</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Idaho</region></placeName><wicri:cityArea>Department of Biological Sciences, Center for Reproductive Biology, University of Idaho, Moscow</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Zoology Part A: Ecological Genetics and Physiology</title><title level="j" type="abbrev">J. Exp. Zool.</title><idno type="ISSN">1932-5223</idno><idno type="eISSN">1932-5231</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2007-10-01">2007-10-01</date><biblScope unit="volume">307A</biblScope><biblScope unit="issue">10</biblScope><biblScope unit="page" from="590">590</biblScope><biblScope unit="page" to="599">599</biblScope></imprint><idno type="ISSN">1932-5223</idno></series><idno type="istex">66309AB3948954F7282E5492A7364529B57887A6</idno><idno type="DOI">10.1002/jez.414</idno><idno type="ArticleID">JEZ414</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1932-5223</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Before dilution in hypoosmotic media sperm of freshwater fish are maintained quiescent by a range of factors including osmolality, K+ and pH, and the onset of motility is generally associated with an increase in cytoplasmic Ca2+. In contrast, Ca2+ in conjunction with osmolality was found to inhibit motility of intact bluegill sperm. Consistent with seminal plasma composition, 0.16 mmol/L Ca2+ and greater, in conjunction with an osmotic concentration of 290 mOsm/kg, inhibited the onset of bluegill sperm motility; sperm diluted in saline at 290 mOsm/kg without Ca2+ became motile. Cations Mn2+ and Sr2+, in conjunction with osmolality, had an inhibitory effect on initiation of sperm motility similar to that of Ca2+. Sperm motility was inhibited by Ca2+ channel blockers nimodipine and nifedipine, the mitochondrial Ca2+ uniporter inhibitor ruthenium red and the calmodulin inhibitors W‐7 and trifluoperazine dihydrochloride. These results provide evidence that elevated cytoplasmic Ca2+ inhibits sperm motility and yet low levels permit or promote motility. This study demonstrates a unique inhibitory action of Ca2+ on the motility of intact fish sperm at physiologically relevant levels. J. Exp. Zool. 307A:590–599, 2007. © 2007 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Idaho</li></region></list><tree><country name="États-Unis"><region name="Idaho"><name sortKey="Zuccarelli, Micah D" sort="Zuccarelli, Micah D" uniqKey="Zuccarelli M" first="Micah D." last="Zuccarelli">Micah D. Zuccarelli</name></region><name sortKey="Ingermann, Rolf L" sort="Ingermann, Rolf L" uniqKey="Ingermann R" first="Rolf L." last="Ingermann">Rolf L. Ingermann</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Eau/explor/LotaV3/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000805 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000805 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Eau
|area= LotaV3
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:66309AB3948954F7282E5492A7364529B57887A6
|texte= Calcium‐induced quiescence of sperm motility in the bluegill (Lepomis macrochirus)
}}
| This area was generated with Dilib version V0.6.39. |  |