Exposure of fish to biologically treated bleached‐kraft effluent. 2. Induction of hepatic cytochrome P4501A in mountain whitefish (Prosopium williamsoni) and other species
Identifieur interne : 000115 ( Istex/Corpus ); précédent : 000114; suivant : 000116Exposure of fish to biologically treated bleached‐kraft effluent. 2. Induction of hepatic cytochrome P4501A in mountain whitefish (Prosopium williamsoni) and other species
Auteurs : P. J. Kloepper-Sams ; E. BentonSource :
- Environmental Toxicology and Chemistry [ 0730-7268 ] ; 1994-09.
English descriptors
Abstract
Induction of the hepatic detoxification enzyme cytochrome P4501A has been observed in fish exposed to bleached‐kraft mill effluents (BKME). P4501A content was examined in three species of wild fish exposed to BKME in a western Canadian river as part of an extensive program that included chemical monitoring, fish population studies, and other fish biochemical and physiological measurements. The Rocky Mountain whitefish Prosopium williamsoni exhibited marked induction of P4501A compared to reference whitefish, as measured by both catalytic activity and immunoreactive protein content. Similar P4501A induction was observed 4 d after reference fish were treated with 20 mg/kg β‐naphthoflavone. Whitefish P4501A levels have declined from a peak in spring 1991, following mill process modifications and concurrent with reductions in body burdens of hydrophobic compounds. Whitefish collected near the mill, moved upstream of effluent discharges, and held for 8 d showed no significant loss of hepatic P4501A‐related (ethoxyresorufin O‐deethylase, EROD) enzyme activity or P4501A protein levels. For spring 1991, correlations were found between EROD activity and measures of chronic exposure to BKME (e.g., muscle 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin content), but not between EROD and measures of acute exposure (e.g., bile content of resin acids or chlorophenolic metabolites). These and other lines of evidence indicate that the P4501A‐inducing agent(s) at this site may be neither waterborne nor rapidly eliminated. A second species, longnose sucker (Catostomus catostomus), collected near the mill exhibited modest (two‐ to threefold) P4501A induction. For both species, no significant correlations between P4501A induction and trends in other biological responses were found, as described in a companion paper. Burbot (Lota lota) had hepatic EROD activities generally in the range of reference values, despite substantial exposure to mill‐related compounds. In contrast to studies at historically degraded pulp mill sites, P4501A induction is the only major biological response observed to date at this site. As P4501A induction is not related to adverse effects, it could be best classified as an indicator of exposure to BKME.
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DOI: 10.1002/etc.5620130911
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Exposure of fish to biologically treated bleached‐kraft effluent. 2. Induction of hepatic cytochrome P4501A in mountain whitefish (Prosopium williamsoni) and other species</title><author><name sortKey="Kloepper Ams, P J" sort="Kloepper Ams, P J" uniqKey="Kloepper Ams P" first="P. J." last="Kloepper-Sams">P. J. Kloepper-Sams</name><affiliation><mods:affiliation>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</mods:affiliation></affiliation><affiliation><mods:affiliation>Current Address: The Procter & Gamble Company, European Technical Center, Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Benton, E" sort="Benton, E" uniqKey="Benton E" first="E." last="Benton">E. Benton</name><affiliation><mods:affiliation>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:21766DAE57CE0D85F9A09923E7C0DC23569E1430</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1002/etc.5620130911</idno><idno type="url">https://api.istex.fr/document/21766DAE57CE0D85F9A09923E7C0DC23569E1430/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000115</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Exposure of fish to biologically treated bleached‐kraft effluent. 2. Induction of hepatic cytochrome P4501A in mountain whitefish (Prosopium williamsoni) and other species</title><author><name sortKey="Kloepper Ams, P J" sort="Kloepper Ams, P J" uniqKey="Kloepper Ams P" first="P. J." last="Kloepper-Sams">P. J. Kloepper-Sams</name><affiliation><mods:affiliation>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</mods:affiliation></affiliation><affiliation><mods:affiliation>Current Address: The Procter & Gamble Company, European Technical Center, Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Benton, E" sort="Benton, E" uniqKey="Benton E" first="E." last="Benton">E. Benton</name><affiliation><mods:affiliation>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Environmental Toxicology and Chemistry</title><title level="j" type="sub">An International Journal</title><title level="j" type="abbrev">Environmental Toxicology and Chemistry</title><idno type="ISSN">0730-7268</idno><idno type="eISSN">1552-8618</idno><imprint><publisher>Wiley Periodicals, Inc.</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1994-09">1994-09</date><biblScope unit="volume">13</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="1483">1483</biblScope><biblScope unit="page" to="1496">1496</biblScope></imprint><idno type="ISSN">0730-7268</idno></series><idno type="istex">21766DAE57CE0D85F9A09923E7C0DC23569E1430</idno><idno type="DOI">10.1002/etc.5620130911</idno><idno type="ArticleID">ETC5620130911</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0730-7268</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bleached‐kraft mill effluent</term><term>Cytochrome P4501A</term><term>EROD activity</term><term>β‐Naphthoflavone TCDD</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Induction of the hepatic detoxification enzyme cytochrome P4501A has been observed in fish exposed to bleached‐kraft mill effluents (BKME). P4501A content was examined in three species of wild fish exposed to BKME in a western Canadian river as part of an extensive program that included chemical monitoring, fish population studies, and other fish biochemical and physiological measurements. The Rocky Mountain whitefish Prosopium williamsoni exhibited marked induction of P4501A compared to reference whitefish, as measured by both catalytic activity and immunoreactive protein content. Similar P4501A induction was observed 4 d after reference fish were treated with 20 mg/kg β‐naphthoflavone. Whitefish P4501A levels have declined from a peak in spring 1991, following mill process modifications and concurrent with reductions in body burdens of hydrophobic compounds. Whitefish collected near the mill, moved upstream of effluent discharges, and held for 8 d showed no significant loss of hepatic P4501A‐related (ethoxyresorufin O‐deethylase, EROD) enzyme activity or P4501A protein levels. For spring 1991, correlations were found between EROD activity and measures of chronic exposure to BKME (e.g., muscle 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin content), but not between EROD and measures of acute exposure (e.g., bile content of resin acids or chlorophenolic metabolites). These and other lines of evidence indicate that the P4501A‐inducing agent(s) at this site may be neither waterborne nor rapidly eliminated. A second species, longnose sucker (Catostomus catostomus), collected near the mill exhibited modest (two‐ to threefold) P4501A induction. For both species, no significant correlations between P4501A induction and trends in other biological responses were found, as described in a companion paper. Burbot (Lota lota) had hepatic EROD activities generally in the range of reference values, despite substantial exposure to mill‐related compounds. In contrast to studies at historically degraded pulp mill sites, P4501A induction is the only major biological response observed to date at this site. As P4501A induction is not related to adverse effects, it could be best classified as an indicator of exposure to BKME.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>P.J. Kloepper‐Sams</name><affiliations><json:string>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</json:string><json:string>Current Address: The Procter & Gamble Company, European Technical Center, Brussels, Belgium</json:string></affiliations></json:item><json:item><name>E. Benton</name><affiliations><json:string>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Bleached‐kraft mill effluent</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Cytochrome P4501A</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>EROD activity</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>β‐Naphthoflavone TCDD</value></json:item></subject><language><json:string>eng</json:string></language><abstract>Induction of the hepatic detoxification enzyme cytochrome P4501A has been observed in fish exposed to bleached‐kraft mill effluents (BKME). P4501A content was examined in three species of wild fish exposed to BKME in a western Canadian river as part of an extensive program that included chemical monitoring, fish population studies, and other fish biochemical and physiological measurements. The Rocky Mountain whitefish Prosopium williamsoni exhibited marked induction of P4501A compared to reference whitefish, as measured by both catalytic activity and immunoreactive protein content. Similar P4501A induction was observed 4 d after reference fish were treated with 20 mg/kg β‐naphthoflavone. Whitefish P4501A levels have declined from a peak in spring 1991, following mill process modifications and concurrent with reductions in body burdens of hydrophobic compounds. Whitefish collected near the mill, moved upstream of effluent discharges, and held for 8 d showed no significant loss of hepatic P4501A‐related (ethoxyresorufin O‐deethylase, EROD) enzyme activity or P4501A protein levels. For spring 1991, correlations were found between EROD activity and measures of chronic exposure to BKME (e.g., muscle 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin content), but not between EROD and measures of acute exposure (e.g., bile content of resin acids or chlorophenolic metabolites). These and other lines of evidence indicate that the P4501A‐inducing agent(s) at this site may be neither waterborne nor rapidly eliminated. A second species, longnose sucker (Catostomus catostomus), collected near the mill exhibited modest (two‐ to threefold) P4501A induction. For both species, no significant correlations between P4501A induction and trends in other biological responses were found, as described in a companion paper. Burbot (Lota lota) had hepatic EROD activities generally in the range of reference values, despite substantial exposure to mill‐related compounds. In contrast to studies at historically degraded pulp mill sites, P4501A induction is the only major biological response observed to date at this site. As P4501A induction is not related to adverse effects, it could be best classified as an indicator of exposure to BKME.</abstract><qualityIndicators><score>8</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>4</keywordCount><abstractCharCount>2233</abstractCharCount><pdfWordCount>9946</pdfWordCount><pdfCharCount>61817</pdfCharCount><pdfPageCount>14</pdfPageCount><abstractWordCount>319</abstractWordCount></qualityIndicators><title>Exposure of fish to biologically treated bleached‐kraft effluent. 2. 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Induction of hepatic cytochrome P4501A in mountain whitefish (Prosopium williamsoni) and other species</title><author><persName><forename type="first">P.J.</forename><surname>Kloepper‐Sams</surname></persName><note type="correspondence"><p>Correspondence: Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</p></note><affiliation>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</affiliation><affiliation>Current Address: The Procter & Gamble Company, European Technical Center, Brussels, Belgium</affiliation></author><author><persName><forename type="first">E.</forename><surname>Benton</surname></persName><affiliation>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</affiliation></author></analytic><monogr><title level="j">Environmental Toxicology and Chemistry</title><title level="j" type="sub">An International Journal</title><title level="j" type="abbrev">Environmental Toxicology and Chemistry</title><idno type="pISSN">0730-7268</idno><idno type="eISSN">1552-8618</idno><idno type="DOI">10.1002/(ISSN)1552-8618</idno><imprint><publisher>Wiley Periodicals, Inc.</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="1994-09"></date><biblScope unit="volume">13</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="1483">1483</biblScope><biblScope unit="page" to="1496">1496</biblScope></imprint></monogr><idno type="istex">21766DAE57CE0D85F9A09923E7C0DC23569E1430</idno><idno type="DOI">10.1002/etc.5620130911</idno><idno type="ArticleID">ETC5620130911</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1994</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Induction of the hepatic detoxification enzyme cytochrome P4501A has been observed in fish exposed to bleached‐kraft mill effluents (BKME). P4501A content was examined in three species of wild fish exposed to BKME in a western Canadian river as part of an extensive program that included chemical monitoring, fish population studies, and other fish biochemical and physiological measurements. The Rocky Mountain whitefish Prosopium williamsoni exhibited marked induction of P4501A compared to reference whitefish, as measured by both catalytic activity and immunoreactive protein content. Similar P4501A induction was observed 4 d after reference fish were treated with 20 mg/kg β‐naphthoflavone. Whitefish P4501A levels have declined from a peak in spring 1991, following mill process modifications and concurrent with reductions in body burdens of hydrophobic compounds. Whitefish collected near the mill, moved upstream of effluent discharges, and held for 8 d showed no significant loss of hepatic P4501A‐related (ethoxyresorufin O‐deethylase, EROD) enzyme activity or P4501A protein levels. For spring 1991, correlations were found between EROD activity and measures of chronic exposure to BKME (e.g., muscle 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin content), but not between EROD and measures of acute exposure (e.g., bile content of resin acids or chlorophenolic metabolites). These and other lines of evidence indicate that the P4501A‐inducing agent(s) at this site may be neither waterborne nor rapidly eliminated. A second species, longnose sucker (Catostomus catostomus), collected near the mill exhibited modest (two‐ to threefold) P4501A induction. For both species, no significant correlations between P4501A induction and trends in other biological responses were found, as described in a companion paper. Burbot (Lota lota) had hepatic EROD activities generally in the range of reference values, despite substantial exposure to mill‐related compounds. In contrast to studies at historically degraded pulp mill sites, P4501A induction is the only major biological response observed to date at this site. As P4501A induction is not related to adverse effects, it could be best classified as an indicator of exposure to BKME.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Bleached‐kraft mill effluent</term></item><item><term>Cytochrome P4501A</term></item><item><term>EROD activity</term></item><item><term>β‐Naphthoflavone TCDD</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article category</head><item><term>Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1993-08-04">Received</change><change when="1994-02-18">Registration</change><change when="1994-09">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/21766DAE57CE0D85F9A09923E7C0DC23569E1430/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Periodicals, Inc.</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1552-8618</doi><issn type="print">0730-7268</issn><issn type="electronic">1552-8618</issn><idGroup><id type="product" value="ETC"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY">Environmental Toxicology and Chemistry</title><title type="subtitle">An International Journal</title><title type="short">Environmental Toxicology and Chemistry</title></titleGroup></publicationMeta><publicationMeta level="part" position="90"><doi origin="wiley" registered="no">10.1002/etc.v13:9</doi><numberingGroup><numbering type="journalVolume" number="13">13</numbering><numbering type="journalIssue">9</numbering></numberingGroup><coverDate startDate="1994-09">September 1994</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="11" status="forIssue"><doi origin="wiley" registered="no">10.1002/etc.5620130911</doi><idGroup><id type="unit" value="ETC5620130911"></id></idGroup><countGroup><count type="pageTotal" number="14"></count></countGroup><titleGroup><title type="articleCategory">Article</title><title type="tocHeading1">Articles</title></titleGroup><copyright ownership="thirdParty">Copyright © 1994 SETAC</copyright><eventGroup><event type="manuscriptReceived" date="1993-08-04"></event><event type="manuscriptAccepted" date="1994-02-18"></event><event type="firstOnline" date="2009-10-20"></event><event type="publishedOnlineFinalForm" date="2009-10-20"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.5 mode:FullText source:HeaderRef result:HeaderRef" date="2010-04-09"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:3.8.8" date="2014-01-25"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-16"></event></eventGroup><numberingGroup><numbering type="pageFirst">1483</numbering><numbering type="pageLast">1496</numbering></numberingGroup><correspondenceTo>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:ETC.ETC5620130911.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="7"></count><count type="tableTotal" number="3"></count><count type="referenceTotal" number="70"></count></countGroup><titleGroup><title type="main" xml:lang="en">Exposure of fish to biologically treated bleached‐kraft effluent. 2. Induction of hepatic cytochrome P4501A in mountain whitefish (<i>Prosopium williamsoni</i>) and other species</title><title type="short" xml:lang="en">P4501A in fish exposed to pulp effluent</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" currentRef="#curr1" corresponding="yes"><personName><givenNames>P.J.</givenNames><familyName>Kloepper‐Sams</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>E.</givenNames><familyName>Benton</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="US" type="organization"><unparsedAffiliation>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</unparsedAffiliation></affiliation><affiliation xml:id="curr1" countryCode="BE"><unparsedAffiliation>The Procter & Gamble Company, European Technical Center, Brussels, Belgium</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">Bleached‐kraft mill effluent</keyword><keyword xml:id="kwd2">Cytochrome P4501A</keyword><keyword xml:id="kwd3">EROD activity</keyword><keyword xml:id="kwd4">β‐Naphthoflavone TCDD</keyword></keywordGroup><fundingInfo><fundingAgency>Procter & Gamble Cellulose</fundingAgency></fundingInfo><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Induction of the hepatic detoxification enzyme cytochrome P4501A has been observed in fish exposed to bleached‐kraft mill effluents (BKME). P4501A content was examined in three species of wild fish exposed to BKME in a western Canadian river as part of an extensive program that included chemical monitoring, fish population studies, and other fish biochemical and physiological measurements. The Rocky Mountain whitefish Prosopium williamsoni exhibited marked induction of P4501A compared to reference whitefish, as measured by both catalytic activity and immunoreactive protein content. Similar P4501A induction was observed 4 d after reference fish were treated with 20 mg/kg β‐naphthoflavone. Whitefish P4501A levels have declined from a peak in spring 1991, following mill process modifications and concurrent with reductions in body burdens of hydrophobic compounds. Whitefish collected near the mill, moved upstream of effluent discharges, and held for 8 d showed no significant loss of hepatic P4501A‐related (ethoxyresorufin O‐deethylase, EROD) enzyme activity or P4501A protein levels. For spring 1991, correlations were found between EROD activity and measures of chronic exposure to BKME (e.g., muscle 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin content), but not between EROD and measures of acute exposure (e.g., bile content of resin acids or chlorophenolic metabolites). These and other lines of evidence indicate that the P4501A‐inducing agent(s) at this site may be neither waterborne nor rapidly eliminated. A second species, longnose sucker (Catostomus catostomus), collected near the mill exhibited modest (two‐ to threefold) P4501A induction. For both species, no significant correlations between P4501A induction and trends in other biological responses were found, as described in a companion paper. Burbot (Lota lota) had hepatic EROD activities generally in the range of reference values, despite substantial exposure to mill‐related compounds. In contrast to studies at historically degraded pulp mill sites, P4501A induction is the only major biological response observed to date at this site. As P4501A induction is not related to adverse effects, it could be best classified as an indicator of exposure to BKME.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Exposure of fish to biologically treated bleached‐kraft effluent. 2. Induction of hepatic cytochrome P4501A in mountain whitefish (Prosopium williamsoni) and other species</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>P4501A in fish exposed to pulp effluent</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Exposure of fish to biologically treated bleached‐kraft effluent. 2. Induction of hepatic cytochrome P4501A in mountain whitefish (</title></titleInfo><name type="personal"><namePart type="given">P.J.</namePart><namePart type="family">Kloepper‐Sams</namePart><affiliation>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</affiliation><affiliation>Current Address: The Procter & Gamble Company, European Technical Center, Brussels, Belgium</affiliation><description>Correspondence: Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">E.</namePart><namePart type="family">Benton</namePart><affiliation>Environmental Science Department, The Procter & Gamble Company, Cincinnati, Ohio 45217</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article">article</genre><originInfo><publisher>Wiley Periodicals, Inc.</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">1994-09</dateIssued><dateCaptured encoding="w3cdtf">1993-08-04</dateCaptured><dateValid encoding="w3cdtf">1994-02-18</dateValid><copyrightDate encoding="w3cdtf">1994</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">7</extent><extent unit="tables">3</extent><extent unit="references">70</extent></physicalDescription><abstract lang="en">Induction of the hepatic detoxification enzyme cytochrome P4501A has been observed in fish exposed to bleached‐kraft mill effluents (BKME). P4501A content was examined in three species of wild fish exposed to BKME in a western Canadian river as part of an extensive program that included chemical monitoring, fish population studies, and other fish biochemical and physiological measurements. The Rocky Mountain whitefish Prosopium williamsoni exhibited marked induction of P4501A compared to reference whitefish, as measured by both catalytic activity and immunoreactive protein content. Similar P4501A induction was observed 4 d after reference fish were treated with 20 mg/kg β‐naphthoflavone. Whitefish P4501A levels have declined from a peak in spring 1991, following mill process modifications and concurrent with reductions in body burdens of hydrophobic compounds. Whitefish collected near the mill, moved upstream of effluent discharges, and held for 8 d showed no significant loss of hepatic P4501A‐related (ethoxyresorufin O‐deethylase, EROD) enzyme activity or P4501A protein levels. For spring 1991, correlations were found between EROD activity and measures of chronic exposure to BKME (e.g., muscle 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin content), but not between EROD and measures of acute exposure (e.g., bile content of resin acids or chlorophenolic metabolites). These and other lines of evidence indicate that the P4501A‐inducing agent(s) at this site may be neither waterborne nor rapidly eliminated. A second species, longnose sucker (Catostomus catostomus), collected near the mill exhibited modest (two‐ to threefold) P4501A induction. For both species, no significant correlations between P4501A induction and trends in other biological responses were found, as described in a companion paper. Burbot (Lota lota) had hepatic EROD activities generally in the range of reference values, despite substantial exposure to mill‐related compounds. In contrast to studies at historically degraded pulp mill sites, P4501A induction is the only major biological response observed to date at this site. As P4501A induction is not related to adverse effects, it could be best classified as an indicator of exposure to BKME.</abstract><note type="funding">Procter & Gamble Cellulose</note><subject lang="en"><genre>Keywords</genre><topic>Bleached‐kraft mill effluent</topic><topic>Cytochrome P4501A</topic><topic>EROD activity</topic><topic>β‐Naphthoflavone TCDD</topic></subject><relatedItem type="host"><titleInfo><title>Environmental Toxicology and Chemistry</title><subTitle>An International Journal</subTitle></titleInfo><titleInfo type="abbreviated"><title>Environmental Toxicology and Chemistry</title></titleInfo><subject><genre>article category</genre><topic>Article</topic></subject><identifier type="ISSN">0730-7268</identifier><identifier type="eISSN">1552-8618</identifier><identifier type="DOI">10.1002/(ISSN)1552-8618</identifier><identifier type="PublisherID">ETC</identifier><part><date>1994</date><detail type="volume"><caption>vol.</caption><number>13</number></detail><detail type="issue"><caption>no.</caption><number>9</number></detail><extent unit="pages"><start>1483</start><end>1496</end><total>14</total></extent></part></relatedItem><identifier type="istex">21766DAE57CE0D85F9A09923E7C0DC23569E1430</identifier><identifier type="DOI">10.1002/etc.5620130911</identifier><identifier type="ArticleID">ETC5620130911</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 1994 SETAC</accessCondition><recordInfo><recordOrigin>Wiley Periodicals, Inc.</recordOrigin><recordContentSource>WILEY</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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