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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (<italic>Mytilus edulis</italic>) hemocytes in vitro</title><author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name><affiliation><nlm:aff id="Aff1">Laboratory of Ecotoxicology, EA 3222, FED 4116 SCALE, University of Le Havre, 76058 Le Havre Cedex, France</nlm:aff></affiliation></author><author><name sortKey="Lebel, Jean Marc" sort="Lebel, Jean Marc" uniqKey="Lebel J" first="Jean-Marc" last="Lebel">Jean-Marc Lebel</name><affiliation><nlm:aff id="Aff2">CNRS INEE, FRE3484 BioMEA Biologie des Mollusques marins et des Ecosystèmes Associés, IBFA, University of Caen, 14032 Caen Cedex, France</nlm:aff></affiliation></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name><affiliation><nlm:aff id="Aff1">Laboratory of Ecotoxicology, EA 3222, FED 4116 SCALE, University of Le Havre, 76058 Le Havre Cedex, France</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23579247</idno><idno type="pmc">3967607</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967607</idno><idno type="RBID">PMC:3967607</idno><idno type="doi">10.1007/s10616-013-9558-2</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000034</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (<italic>Mytilus edulis</italic>) hemocytes in vitro</title><author><name sortKey="Rioult, Damien" sort="Rioult, Damien" uniqKey="Rioult D" first="Damien" last="Rioult">Damien Rioult</name><affiliation><nlm:aff id="Aff1">Laboratory of Ecotoxicology, EA 3222, FED 4116 SCALE, University of Le Havre, 76058 Le Havre Cedex, France</nlm:aff></affiliation></author><author><name sortKey="Lebel, Jean Marc" sort="Lebel, Jean Marc" uniqKey="Lebel J" first="Jean-Marc" last="Lebel">Jean-Marc Lebel</name><affiliation><nlm:aff id="Aff2">CNRS INEE, FRE3484 BioMEA Biologie des Mollusques marins et des Ecosystèmes Associés, IBFA, University of Caen, 14032 Caen Cedex, France</nlm:aff></affiliation></author><author><name sortKey="Le Foll, Frank" sort="Le Foll, Frank" uniqKey="Le Foll F" first="Frank" last="Le Foll">Frank Le Foll</name><affiliation><nlm:aff id="Aff1">Laboratory of Ecotoxicology, EA 3222, FED 4116 SCALE, University of Le Havre, 76058 Le Havre Cedex, France</nlm:aff></affiliation></author></analytic><series><title level="j">Cytotechnology</title><idno type="ISSN">0920-9069</idno><idno type="eISSN">1573-0778</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Hemocytes constitute the key element of innate immunity in bivalves, being responsible for secretion of antimicrobial peptides and release of zymogens from the prophenoloxidase system within the hemolymph compartment, reactive oxygen species production and phagocytosis. Hemocytes are found (and collected) as cells in suspension in circulating hemolymph. Hemocytes are adherent cells as well, infiltrating tissues and migrating to infected areas. In the present study, we applied an approach based on fluorescent staining and nuclei-tracking to determine migration velocity of hemocytes from the blue mussel, <italic>Mytilus edulis</italic>, in culture. Freshly collected hemocytes attached to substrate and start to move spontaneously in few minutes. Two main hemocyte morphologies can be observed: small star-shaped cells which were less motile and spread granular cells with faster migrations. Cell-tracking was combined to MTT mitochondria metabolic rate measurements in order to monitor global cell population activity over 4 days of culture. A transient peak of cell activity was recorded after 24–48 h of culture, corresponding to a speed up of cell migration. Videomicroscopy and cell tracking techniques provide new tools to characterize activity of mussel immunocytes in culture. Our analysis of hemocyte migration reveals that motility is very sensitive to cell environmental factors.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s10616-013-9558-2) contains supplementary material, which is available to authorized users.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Cytotechnology</journal-id><journal-id journal-id-type="iso-abbrev">Cytotechnology</journal-id><journal-title-group><journal-title>Cytotechnology</journal-title></journal-title-group><issn pub-type="ppub">0920-9069</issn><issn pub-type="epub">1573-0778</issn><publisher><publisher-name>Springer Netherlands</publisher-name><publisher-loc>Dordrecht</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23579247</article-id><article-id pub-id-type="pmc">3967607</article-id><article-id pub-id-type="publisher-id">9558</article-id><article-id pub-id-type="doi">10.1007/s10616-013-9558-2</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Research</subject></subj-group></article-categories><title-group><article-title>Cell tracking and velocimetric parameters analysis as an approach to assess activity of mussel (<italic>Mytilus edulis</italic>) hemocytes in vitro</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Rioult</surname><given-names>Damien</given-names></name><address><phone>+33-2-32744304</phone><fax>+33-2-32744505</fax><email>drioult@free.fr</email><email>damien.rioult@univ-lehavre.fr</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Lebel</surname><given-names>Jean-Marc</given-names></name><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Le Foll</surname><given-names>Frank</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1"><label></label>Laboratory of Ecotoxicology, EA 3222, FED 4116 SCALE, University of Le Havre, 76058 Le Havre Cedex, France</aff><aff id="Aff2"><label></label>CNRS INEE, FRE3484 BioMEA Biologie des Mollusques marins et des Ecosystèmes Associés, IBFA, University of Caen, 14032 Caen Cedex, France</aff></contrib-group><pub-date pub-type="epub"><day>12</day><month>4</month><year>2013</year></pub-date><pub-date pub-type="ppub"><month>10</month><year>2013</year></pub-date><volume>65</volume><issue>5</issue><fpage>749</fpage><lpage>758</lpage><history><date date-type="received"><day>23</day><month>11</month><year>2012</year></date><date date-type="accepted"><day>22</day><month>3</month><year>2013</year></date></history><permissions><copyright-statement>© Springer Science+Business Media Dordrecht 2013</copyright-statement></permissions><abstract id="Abs1"><p>Hemocytes constitute the key element of innate immunity in bivalves, being responsible for secretion of antimicrobial peptides and release of zymogens from the prophenoloxidase system within the hemolymph compartment, reactive oxygen species production and phagocytosis. Hemocytes are found (and collected) as cells in suspension in circulating hemolymph. Hemocytes are adherent cells as well, infiltrating tissues and migrating to infected areas. In the present study, we applied an approach based on fluorescent staining and nuclei-tracking to determine migration velocity of hemocytes from the blue mussel, <italic>Mytilus edulis</italic>, in culture. Freshly collected hemocytes attached to substrate and start to move spontaneously in few minutes. Two main hemocyte morphologies can be observed: small star-shaped cells which were less motile and spread granular cells with faster migrations. Cell-tracking was combined to MTT mitochondria metabolic rate measurements in order to monitor global cell population activity over 4 days of culture. A transient peak of cell activity was recorded after 24–48 h of culture, corresponding to a speed up of cell migration. Videomicroscopy and cell tracking techniques provide new tools to characterize activity of mussel immunocytes in culture. Our analysis of hemocyte migration reveals that motility is very sensitive to cell environmental factors.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s10616-013-9558-2) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Marine Invertebrate</kwd><kwd>Primary cultures</kwd><kwd>Motility</kwd><kwd>Cell-tracking</kwd><kwd>Innate Immunity</kwd><kwd>Molluscs</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer Science+Business Media Dordrecht 2013</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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