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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular characterization and mRNA expression of <italic>grp78</italic> and <italic>hsp90A</italic> in the estuarine copepod <italic>Eurytemora affinis</italic></title><author><name sortKey="Xuereb, Benoit" sort="Xuereb, Benoit" uniqKey="Xuereb B" first="Benoit" last="Xuereb">Benoit Xuereb</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Forget Leray, Joelle" sort="Forget Leray, Joelle" uniqKey="Forget Leray J" first="Joëlle" last="Forget-Leray">Joëlle Forget-Leray</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Souissi, Sami" sort="Souissi, Sami" uniqKey="Souissi S" first="Sami" last="Souissi">Sami Souissi</name><affiliation><nlm:aff id="Aff2">Université Lille Nord de France, 59000 Lille, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3">USTL, LOG, Station Marine de Wimereux, 62930 Wimereux, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">CNRS, UMR 8187, 62930 Wimereux, France</nlm:aff></affiliation></author><author><name sortKey="Glippa, Olivier" sort="Glippa, Olivier" uniqKey="Glippa O" first="Olivier" last="Glippa">Olivier Glippa</name><affiliation><nlm:aff id="Aff2">Université Lille Nord de France, 59000 Lille, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3">USTL, LOG, Station Marine de Wimereux, 62930 Wimereux, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">CNRS, UMR 8187, 62930 Wimereux, France</nlm:aff></affiliation></author><author><name sortKey="Devreker, David" sort="Devreker, David" uniqKey="Devreker D" first="David" last="Devreker">David Devreker</name><affiliation><nlm:aff id="Aff2">Université Lille Nord de France, 59000 Lille, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3">USTL, LOG, Station Marine de Wimereux, 62930 Wimereux, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">CNRS, UMR 8187, 62930 Wimereux, France</nlm:aff></affiliation></author><author><name sortKey="Lesueur, Teddy" sort="Lesueur, Teddy" uniqKey="Lesueur T" first="Teddy" last="Lesueur">Teddy Lesueur</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Marie, Sabine" sort="Marie, Sabine" uniqKey="Marie S" first="Sabine" last="Marie">Sabine Marie</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Boulange Lecomte, Celine" sort="Boulange Lecomte, Celine" uniqKey="Boulange Lecomte C" first="Céline" last="Boulangé-Lecomte">Céline Boulangé-Lecomte</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22302500</idno><idno type="pmc">3368034</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368034</idno><idno type="RBID">PMC:3368034</idno><idno type="doi">10.1007/s12192-012-0323-9</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">000032</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular characterization and mRNA expression of <italic>grp78</italic> and <italic>hsp90A</italic> in the estuarine copepod <italic>Eurytemora affinis</italic></title><author><name sortKey="Xuereb, Benoit" sort="Xuereb, Benoit" uniqKey="Xuereb B" first="Benoit" last="Xuereb">Benoit Xuereb</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Forget Leray, Joelle" sort="Forget Leray, Joelle" uniqKey="Forget Leray J" first="Joëlle" last="Forget-Leray">Joëlle Forget-Leray</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Souissi, Sami" sort="Souissi, Sami" uniqKey="Souissi S" first="Sami" last="Souissi">Sami Souissi</name><affiliation><nlm:aff id="Aff2">Université Lille Nord de France, 59000 Lille, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3">USTL, LOG, Station Marine de Wimereux, 62930 Wimereux, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">CNRS, UMR 8187, 62930 Wimereux, France</nlm:aff></affiliation></author><author><name sortKey="Glippa, Olivier" sort="Glippa, Olivier" uniqKey="Glippa O" first="Olivier" last="Glippa">Olivier Glippa</name><affiliation><nlm:aff id="Aff2">Université Lille Nord de France, 59000 Lille, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3">USTL, LOG, Station Marine de Wimereux, 62930 Wimereux, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">CNRS, UMR 8187, 62930 Wimereux, France</nlm:aff></affiliation></author><author><name sortKey="Devreker, David" sort="Devreker, David" uniqKey="Devreker D" first="David" last="Devreker">David Devreker</name><affiliation><nlm:aff id="Aff2">Université Lille Nord de France, 59000 Lille, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff3">USTL, LOG, Station Marine de Wimereux, 62930 Wimereux, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">CNRS, UMR 8187, 62930 Wimereux, France</nlm:aff></affiliation></author><author><name sortKey="Lesueur, Teddy" sort="Lesueur, Teddy" uniqKey="Lesueur T" first="Teddy" last="Lesueur">Teddy Lesueur</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Marie, Sabine" sort="Marie, Sabine" uniqKey="Marie S" first="Sabine" last="Marie">Sabine Marie</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Danger, Jean Michel" sort="Danger, Jean Michel" uniqKey="Danger J" first="Jean-Michel" last="Danger">Jean-Michel Danger</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author><author><name sortKey="Boulange Lecomte, Celine" sort="Boulange Lecomte, Celine" uniqKey="Boulange Lecomte C" first="Céline" last="Boulangé-Lecomte">Céline Boulangé-Lecomte</name><affiliation><nlm:aff id="Aff1">Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</nlm:aff></affiliation></author></analytic><series><title level="j">Cell Stress & Chaperones</title><idno type="ISSN">1355-8145</idno><idno type="eISSN">1466-1268</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The present study aimed to develop a method of quantification of heat shock protein transcript levels in the estuarine copepod <italic>Eurytemora affinis</italic>. For that, the full-length cDNA of the 78-kDa glucose-regulated protein (<italic>Ea-grp78</italic>) and the cytosolic 90-kDa heat shock protein (<italic>Ea-hsp90A</italic>) from this species have been cloned. These cDNA revealed, respectively, 2,370 and 2,299 bp with 1,971 and 2,124 bp open reading frames encoding 656 and 707 amino acids. Main features, sequence identities and phylogenetic analysis with other species were described. Then, the expression profiles were analysed using reverse transcription/real-time quantitative PCR method from copepods subjected to different thermic and osmotic stresses in laboratory, and from copepods directly sampled into the natural population of the Seine Estuary (France) along a salinity gradient. Thermic shock (7.5°C, 22.5°C and 30°C during 90 min) significantly induced increases of transcript quantities ranged between 1.7- and 19.7-fold the levels observed in control conditions (15°C). Hypo- and hyper-osmotic shocks (salinities of 1 and 30 during 90 min) caused a 2-fold induction of <italic>Ea-hsp90A</italic> transcript level in comparison to controls (salinity of 15) whereas no significant change was measured for <italic>Ea-grp78</italic>. On the other hand, similar expression profiles were observed for the two transcripts after 72 h of exposition to salinities of 1 and 25 with a significant 2-fold induction observed for the lower salinity. To finish, strong expression inductions of both <italic>Ea-grp78</italic> and <italic>Ea-hsp90A</italic> genes were observed in field copepods sampled at low salinity during the campaigns of June 2009 and May 2010. These results tend to show that the low salinity and the increase of temperature seem to have a synergic effect on stress condition of copepods.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Cell Stress Chaperones</journal-id><journal-id journal-id-type="iso-abbrev">Cell Stress Chaperones</journal-id><journal-title-group><journal-title>Cell Stress & Chaperones</journal-title></journal-title-group><issn pub-type="ppub">1355-8145</issn><issn pub-type="epub">1466-1268</issn><publisher><publisher-name>Springer Netherlands</publisher-name><publisher-loc>Dordrecht</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22302500</article-id><article-id pub-id-type="pmc">3368034</article-id><article-id pub-id-type="publisher-id">323</article-id><article-id pub-id-type="doi">10.1007/s12192-012-0323-9</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Paper</subject></subj-group></article-categories><title-group><article-title>Molecular characterization and mRNA expression of <italic>grp78</italic> and <italic>hsp90A</italic> in the estuarine copepod <italic>Eurytemora affinis</italic></article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Xuereb</surname><given-names>Benoit</given-names></name><address><phone>+33-2-32744390</phone><fax>+33-2-32744505</fax><email>benoit.xuereb@univ-lehavre.fr</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Forget-Leray</surname><given-names>Joëlle</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Souissi</surname><given-names>Sami</given-names></name><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Glippa</surname><given-names>Olivier</given-names></name><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Devreker</surname><given-names>David</given-names></name><xref ref-type="aff" rid="Aff2">2</xref><xref ref-type="aff" rid="Aff3">3</xref><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Lesueur</surname><given-names>Teddy</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Marie</surname><given-names>Sabine</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Danger</surname><given-names>Jean-Michel</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Boulangé-Lecomte</surname><given-names>Céline</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label>Faculté des Sciences et Techniques du Havre, Laboratoire d’Ecotoxicologie-Milieux Aquatiques (UPRES EA3222), Fédération SCALE 4116, GDR Ifremer-INRA EXECO, 25 Rue Philippe Lebon, 76058 Le Havre, France</aff><aff id="Aff2"><label>2</label>Université Lille Nord de France, 59000 Lille, France</aff><aff id="Aff3"><label>3</label>USTL, LOG, Station Marine de Wimereux, 62930 Wimereux, France</aff><aff id="Aff4"><label>4</label>CNRS, UMR 8187, 62930 Wimereux, France</aff></contrib-group><pub-date pub-type="epub"><day>1</day><month>2</month><year>2012</year></pub-date><pub-date pub-type="ppub"><month>7</month><year>2012</year></pub-date><volume>17</volume><issue>4</issue><fpage>457</fpage><lpage>472</lpage><history><date date-type="received"><day>1</day><month>9</month><year>2011</year></date><date date-type="rev-recd"><day>9</day><month>12</month><year>2011</year></date><date date-type="accepted"><day>3</day><month>1</month><year>2012</year></date></history><permissions><copyright-statement>© Cell Stress Society International 2012</copyright-statement></permissions><abstract id="Abs1"><p>The present study aimed to develop a method of quantification of heat shock protein transcript levels in the estuarine copepod <italic>Eurytemora affinis</italic>. For that, the full-length cDNA of the 78-kDa glucose-regulated protein (<italic>Ea-grp78</italic>) and the cytosolic 90-kDa heat shock protein (<italic>Ea-hsp90A</italic>) from this species have been cloned. These cDNA revealed, respectively, 2,370 and 2,299 bp with 1,971 and 2,124 bp open reading frames encoding 656 and 707 amino acids. Main features, sequence identities and phylogenetic analysis with other species were described. Then, the expression profiles were analysed using reverse transcription/real-time quantitative PCR method from copepods subjected to different thermic and osmotic stresses in laboratory, and from copepods directly sampled into the natural population of the Seine Estuary (France) along a salinity gradient. Thermic shock (7.5°C, 22.5°C and 30°C during 90 min) significantly induced increases of transcript quantities ranged between 1.7- and 19.7-fold the levels observed in control conditions (15°C). Hypo- and hyper-osmotic shocks (salinities of 1 and 30 during 90 min) caused a 2-fold induction of <italic>Ea-hsp90A</italic> transcript level in comparison to controls (salinity of 15) whereas no significant change was measured for <italic>Ea-grp78</italic>. On the other hand, similar expression profiles were observed for the two transcripts after 72 h of exposition to salinities of 1 and 25 with a significant 2-fold induction observed for the lower salinity. To finish, strong expression inductions of both <italic>Ea-grp78</italic> and <italic>Ea-hsp90A</italic> genes were observed in field copepods sampled at low salinity during the campaigns of June 2009 and May 2010. These results tend to show that the low salinity and the increase of temperature seem to have a synergic effect on stress condition of copepods.</p></abstract><kwd-group><title>Keywords</title><kwd>Abiotic stress</kwd><kwd>Copepod</kwd><kwd>Estuary</kwd><kwd>Glucose-regulated protein 78</kwd><kwd>Heat shock protein 90</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Cell Stress Society International 2012</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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