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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An intron-containing, heat-inducible stress-70 gene in the millipede <italic>Tachypodoiulus niger</italic> (Julidae, Diplopoda)</title><author><name sortKey="Knigge, Thomas" sort="Knigge, Thomas" uniqKey="Knigge T" first="Thomas" last="Knigge">Thomas Knigge</name><affiliation><nlm:aff id="Aff1">Laboratory of Ecotoxicology, EA 3222 PRES Normandie, Le Havre University, 25 Rue Philippe Lebon, F-76058 Le Havre Cedex, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">Laboratoire d’Ecotoxicologie, Université du Havre, 25, rue Ph. Lebon – CS 80540, F-76058 Le Havre Cedex, France</nlm:aff></affiliation></author><author><name sortKey="Bachmann, Lutz" sort="Bachmann, Lutz" uniqKey="Bachmann L" first="Lutz" last="Bachmann">Lutz Bachmann</name><affiliation><nlm:aff id="Aff2">Department of Research and Collections, Natural History Museum, University of Oslo, P.O. Box 1172 Blindern, 0318 Oslo, Norway</nlm:aff></affiliation></author><author><name sortKey="Kohler, Heinz R" sort="Kohler, Heinz R" uniqKey="Kohler H" first="Heinz-R." last="Köhler">Heinz-R. Köhler</name><affiliation><nlm:aff id="Aff3">Animal Physiological Ecology, Institute of Evolution and Ecology, University of Tübingen, Konrad-Adenauer-Str. 20, D-72072 Tübingen, Germany</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24446070</idno><idno type="pmc">4147066</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147066</idno><idno type="RBID">PMC:4147066</idno><idno type="doi">10.1007/s12192-014-0494-7</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000026</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An intron-containing, heat-inducible stress-70 gene in the millipede <italic>Tachypodoiulus niger</italic> (Julidae, Diplopoda)</title><author><name sortKey="Knigge, Thomas" sort="Knigge, Thomas" uniqKey="Knigge T" first="Thomas" last="Knigge">Thomas Knigge</name><affiliation><nlm:aff id="Aff1">Laboratory of Ecotoxicology, EA 3222 PRES Normandie, Le Havre University, 25 Rue Philippe Lebon, F-76058 Le Havre Cedex, France</nlm:aff></affiliation><affiliation><nlm:aff id="Aff4">Laboratoire d’Ecotoxicologie, Université du Havre, 25, rue Ph. Lebon – CS 80540, F-76058 Le Havre Cedex, France</nlm:aff></affiliation></author><author><name sortKey="Bachmann, Lutz" sort="Bachmann, Lutz" uniqKey="Bachmann L" first="Lutz" last="Bachmann">Lutz Bachmann</name><affiliation><nlm:aff id="Aff2">Department of Research and Collections, Natural History Museum, University of Oslo, P.O. Box 1172 Blindern, 0318 Oslo, Norway</nlm:aff></affiliation></author><author><name sortKey="Kohler, Heinz R" sort="Kohler, Heinz R" uniqKey="Kohler H" first="Heinz-R." last="Köhler">Heinz-R. Köhler</name><affiliation><nlm:aff id="Aff3">Animal Physiological Ecology, Institute of Evolution and Ecology, University of Tübingen, Konrad-Adenauer-Str. 20, D-72072 Tübingen, Germany</nlm:aff></affiliation></author></analytic><series><title level="j">Cell Stress & Chaperones</title><idno type="ISSN">1355-8145</idno><idno type="eISSN">1466-1268</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The highly conserved part of the nucleotide-binding domain of the <italic>hsp70</italic> gene family was amplified from the soil diplopod <italic>Tachypodoiulus niger</italic> (Julidae, Diplopoda). Genomic DNA yielded 701, 549 and 540 bp sequences, whereas cDNA from heat shocked animals produced only one distinct fragment of 543 bp. The sequences could be classified as a 70 kDa heat shock protein (<italic>hsp70</italic>), the corresponding 70 kDa heat shock cognate (<italic>hsc70</italic>) and a glucose-related <italic>hsp70</italic> homologue (<italic>grp78</italic>). Comparisons of genomic and cDNA sequences of <italic>hsc70</italic> identified two introns within the consensus sequence. Generally, <italic>stress-70</italic> expression levels were low, which hampered successful RT-PCR and subsequent subcloning. Following experimental heat shock, however, the spliced <italic>hsc70</italic> was amplified predominantly, instead of its inducible homologue <italic>hsp70</italic>. This finding suggests that microevolution in this soil-dwelling arthropod is directed towards low constitutive stress-70 levels and that the capacity for stress-70 induction presumably is limited. <italic>hsc70</italic>, albeit having introns, apparently is inducible and contributes to the stress-70 response.</p></div></front></TEI><pmc article-type="brief-report"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Cell Stress Chaperones</journal-id><journal-id journal-id-type="iso-abbrev">Cell Stress Chaperones</journal-id><journal-title-group><journal-title>Cell Stress & Chaperones</journal-title></journal-title-group><issn pub-type="ppub">1355-8145</issn><issn pub-type="epub">1466-1268</issn><publisher><publisher-name>Springer Netherlands</publisher-name><publisher-loc>Dordrecht</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24446070</article-id><article-id pub-id-type="pmc">4147066</article-id><article-id pub-id-type="publisher-id">494</article-id><article-id pub-id-type="doi">10.1007/s12192-014-0494-7</article-id><article-categories><subj-group subj-group-type="heading"><subject>Short Communication</subject></subj-group></article-categories><title-group><article-title>An intron-containing, heat-inducible stress-70 gene in the millipede <italic>Tachypodoiulus niger</italic> (Julidae, Diplopoda)</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Knigge</surname><given-names>Thomas</given-names></name><address><phone>+33-2-32859904</phone><fax>+33-2-32744314</fax><email>Thomas.Knigge@univ-lehavre.fr</email></address><xref ref-type="aff" rid="Aff1"></xref><xref ref-type="aff" rid="Aff4"></xref></contrib><contrib contrib-type="author"><name><surname>Bachmann</surname><given-names>Lutz</given-names></name><xref ref-type="aff" rid="Aff2"></xref></contrib><contrib contrib-type="author"><name><surname>Köhler</surname><given-names>Heinz-R.</given-names></name><xref ref-type="aff" rid="Aff3"></xref></contrib><aff id="Aff1"><label></label>Laboratory of Ecotoxicology, EA 3222 PRES Normandie, Le Havre University, 25 Rue Philippe Lebon, F-76058 Le Havre Cedex, France</aff><aff id="Aff2"><label></label>Department of Research and Collections, Natural History Museum, University of Oslo, P.O. Box 1172 Blindern, 0318 Oslo, Norway</aff><aff id="Aff3"><label></label>Animal Physiological Ecology, Institute of Evolution and Ecology, University of Tübingen, Konrad-Adenauer-Str. 20, D-72072 Tübingen, Germany</aff><aff id="Aff4"><label></label>Laboratoire d’Ecotoxicologie, Université du Havre, 25, rue Ph. Lebon – CS 80540, F-76058 Le Havre Cedex, France</aff></contrib-group><pub-date pub-type="epub"><day>21</day><month>1</month><year>2014</year></pub-date><pub-date pub-type="ppub"><month>9</month><year>2014</year></pub-date><volume>19</volume><issue>5</issue><fpage>741</fpage><lpage>747</lpage><history><date date-type="received"><day>8</day><month>10</month><year>2013</year></date><date date-type="rev-recd"><day>27</day><month>12</month><year>2013</year></date><date date-type="accepted"><day>1</day><month>1</month><year>2014</year></date></history><permissions><copyright-statement>© Cell Stress Society International 2014</copyright-statement></permissions><abstract id="Abs1"><p>The highly conserved part of the nucleotide-binding domain of the <italic>hsp70</italic> gene family was amplified from the soil diplopod <italic>Tachypodoiulus niger</italic> (Julidae, Diplopoda). Genomic DNA yielded 701, 549 and 540 bp sequences, whereas cDNA from heat shocked animals produced only one distinct fragment of 543 bp. The sequences could be classified as a 70 kDa heat shock protein (<italic>hsp70</italic>), the corresponding 70 kDa heat shock cognate (<italic>hsc70</italic>) and a glucose-related <italic>hsp70</italic> homologue (<italic>grp78</italic>). Comparisons of genomic and cDNA sequences of <italic>hsc70</italic> identified two introns within the consensus sequence. Generally, <italic>stress-70</italic> expression levels were low, which hampered successful RT-PCR and subsequent subcloning. Following experimental heat shock, however, the spliced <italic>hsc70</italic> was amplified predominantly, instead of its inducible homologue <italic>hsp70</italic>. This finding suggests that microevolution in this soil-dwelling arthropod is directed towards low constitutive stress-70 levels and that the capacity for stress-70 induction presumably is limited. <italic>hsc70</italic>, albeit having introns, apparently is inducible and contributes to the stress-70 response.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Stress response</kwd><kwd>Soil invertebrate</kwd><kwd>Arthropods</kwd><kwd>Heat shock proteins</kwd><kwd>Heat shock cognates</kwd><kwd>Glucose-related proteins</kwd><kwd>Biomarker</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Cell Stress Society International 2014</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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