Humoral immunoglobulins of the white sturgeon, Acipenser transmontanus: partial characterization of and recognition with monoclonal antibodies.
Identifieur interne : 000686 ( PubMed/Curation ); précédent : 000685; suivant : 000687Humoral immunoglobulins of the white sturgeon, Acipenser transmontanus: partial characterization of and recognition with monoclonal antibodies.
Auteurs : M A Adkison [États-Unis] ; B. Basurco ; R P HedrickSource :
- Developmental and comparative immunology [ 0145-305X ]
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Anti-Idiotypic (chemistry), Antibodies, Anti-Idiotypic (immunology), Antibodies, Monoclonal (chemistry), Enzyme-Linked Immunosorbent Assay, Epitopes (immunology), Fishes, Flow Cytometry, Glycosylation, Immunoglobulin M (immunology), Immunoglobulins (biosynthesis), Immunoglobulins (chemistry), Immunoglobulins (immunology), Molecular Sequence Data, Molecular Weight, Sequence Analysis.
- MESH :
- chemical , biosynthesis : Immunoglobulins.
- chemical , chemistry : Antibodies, Anti-Idiotypic, Antibodies, Monoclonal, Immunoglobulins.
- chemical , immunology : Antibodies, Anti-Idiotypic, Epitopes, Immunoglobulin M, Immunoglobulins.
- Amino Acid Sequence, Animals, Enzyme-Linked Immunosorbent Assay, Fishes, Flow Cytometry, Glycosylation, Molecular Sequence Data, Molecular Weight, Sequence Analysis.
Abstract
White sturgeon (Acipenser transmontanus) immunoglobulin (Ig) was purified from serum by two methods, ion-exchange chromatography and gel filtration and precipitation of the euglobulin fraction. The purity of these immunoglobulin preparations was confirmed by gel electrophoresis. Sequence analysis of the N-terminal amino acids confirmed that the purified protein was immunoglobulin. The major portion of the immunoglobulin preparation consisted of two proteins with estimated molecular weights (m.w.) of 870 and 170 kDa. The m.w. of the H- and L-chains of the purified Ig were 73 and 27-30 kDa, respectively, as determined by SDS-PAGE. Ion-exchange purified Ig was used to immunize mice for the production of monoclonal antibodies. This resulted in the production of six stable hybrids that recognized sturgeon Ig, two specific for heavy chain and four specific for light chain. The two anti-H-chain mabs were highly specific for white sturgeon Ig while all four anti-L-chain mabs cross reacted with Ig from green sturgeon (A. medirostris), Atlantic sturgeon (A. oxyrhynchus oxyrhynchus), shovelnose sturgeon (Scaphirhynchus platorynchus), and paddlefish (Polyodon spathula), (all Chondrosteans), but not with channel catfish (Ictalurus punctatus), rainbow trout (Oncorhynchus mykiss) or striped bass (Morone saxatilis). The mabs were used to enumerate the percentage of sIg+ lymphocytes in the peripheral blood of white sturgeon by flow cytometry. The percentage of cells positively stained with the mabs ranged from 12 to 28%. In a comparison of mabs with polyclonal rabbit anti-sturgeon Ig serum by ELISA the mabs produced a larger signal and less background than the polyclonal serum.
PubMed: 8915630
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Humoral immunoglobulins of the white sturgeon, Acipenser transmontanus: partial characterization of and recognition with monoclonal antibodies.</title><author><name sortKey="Adkison, M A" sort="Adkison, M A" uniqKey="Adkison M" first="M A" last="Adkison">M A Adkison</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616</wicri:regionArea></affiliation></author><author><name sortKey="Basurco, B" sort="Basurco, B" uniqKey="Basurco B" first="B" last="Basurco">B. Basurco</name></author><author><name sortKey="Hedrick, R P" sort="Hedrick, R P" uniqKey="Hedrick R" first="R P" last="Hedrick">R P Hedrick</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>1996 Jul-Aug</MedlineDate></PubDate></date><idno type="RBID">pubmed:8915630</idno><idno type="pmid">8915630</idno><idno type="wicri:Area/PubMed/Corpus">000686</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000686</idno><idno type="wicri:Area/PubMed/Curation">000686</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000686</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Humoral immunoglobulins of the white sturgeon, Acipenser transmontanus: partial characterization of and recognition with monoclonal antibodies.</title><author><name sortKey="Adkison, M A" sort="Adkison, M A" uniqKey="Adkison M" first="M A" last="Adkison">M A Adkison</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, School of Veterinary Medicine, University of California, Davis 95616</wicri:regionArea></affiliation></author><author><name sortKey="Basurco, B" sort="Basurco, B" uniqKey="Basurco B" first="B" last="Basurco">B. Basurco</name></author><author><name sortKey="Hedrick, R P" sort="Hedrick, R P" uniqKey="Hedrick R" first="R P" last="Hedrick">R P Hedrick</name></author></analytic><series><title level="j">Developmental and comparative immunology</title><idno type="ISSN">0145-305X</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Anti-Idiotypic (chemistry)</term><term>Antibodies, Anti-Idiotypic (immunology)</term><term>Antibodies, Monoclonal (chemistry)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitopes (immunology)</term><term>Fishes</term><term>Flow Cytometry</term><term>Glycosylation</term><term>Immunoglobulin M (immunology)</term><term>Immunoglobulins (biosynthesis)</term><term>Immunoglobulins (chemistry)</term><term>Immunoglobulins (immunology)</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Sequence Analysis</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Immunoglobulins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antibodies, Anti-Idiotypic</term><term>Antibodies, Monoclonal</term><term>Immunoglobulins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Anti-Idiotypic</term><term>Epitopes</term><term>Immunoglobulin M</term><term>Immunoglobulins</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Fishes</term><term>Flow Cytometry</term><term>Glycosylation</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Sequence Analysis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">White sturgeon (Acipenser transmontanus) immunoglobulin (Ig) was purified from serum by two methods, ion-exchange chromatography and gel filtration and precipitation of the euglobulin fraction. The purity of these immunoglobulin preparations was confirmed by gel electrophoresis. Sequence analysis of the N-terminal amino acids confirmed that the purified protein was immunoglobulin. The major portion of the immunoglobulin preparation consisted of two proteins with estimated molecular weights (m.w.) of 870 and 170 kDa. The m.w. of the H- and L-chains of the purified Ig were 73 and 27-30 kDa, respectively, as determined by SDS-PAGE. Ion-exchange purified Ig was used to immunize mice for the production of monoclonal antibodies. This resulted in the production of six stable hybrids that recognized sturgeon Ig, two specific for heavy chain and four specific for light chain. The two anti-H-chain mabs were highly specific for white sturgeon Ig while all four anti-L-chain mabs cross reacted with Ig from green sturgeon (A. medirostris), Atlantic sturgeon (A. oxyrhynchus oxyrhynchus), shovelnose sturgeon (Scaphirhynchus platorynchus), and paddlefish (Polyodon spathula), (all Chondrosteans), but not with channel catfish (Ictalurus punctatus), rainbow trout (Oncorhynchus mykiss) or striped bass (Morone saxatilis). The mabs were used to enumerate the percentage of sIg+ lymphocytes in the peripheral blood of white sturgeon by flow cytometry. The percentage of cells positively stained with the mabs ranged from 12 to 28%. In a comparison of mabs with polyclonal rabbit anti-sturgeon Ig serum by ELISA the mabs produced a larger signal and less background than the polyclonal serum.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8915630</PMID><DateCreated><Year>1997</Year><Month>02</Month><Day>06</Day></DateCreated><DateCompleted><Year>1997</Year><Month>02</Month><Day>06</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0145-305X</ISSN><JournalIssue CitedMedium="Print"><Volume>20</Volume><Issue>4</Issue><PubDate><MedlineDate>1996 Jul-Aug</MedlineDate></PubDate></JournalIssue><Title>Developmental and comparative immunology</Title><ISOAbbreviation>Dev. Comp. 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Ion-exchange purified Ig was used to immunize mice for the production of monoclonal antibodies. This resulted in the production of six stable hybrids that recognized sturgeon Ig, two specific for heavy chain and four specific for light chain. The two anti-H-chain mabs were highly specific for white sturgeon Ig while all four anti-L-chain mabs cross reacted with Ig from green sturgeon (A. medirostris), Atlantic sturgeon (A. oxyrhynchus oxyrhynchus), shovelnose sturgeon (Scaphirhynchus platorynchus), and paddlefish (Polyodon spathula), (all Chondrosteans), but not with channel catfish (Ictalurus punctatus), rainbow trout (Oncorhynchus mykiss) or striped bass (Morone saxatilis). The mabs were used to enumerate the percentage of sIg+ lymphocytes in the peripheral blood of white sturgeon by flow cytometry. The percentage of cells positively stained with the mabs ranged from 12 to 28%. 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