[Effects of cycloheximide, aminoglutethimide, indomethacin, cytochalasin B and colchicine on ovulation and the ultrastructure of the ovarian follicle wall in the stellate sturgeon Acipenser stellatus Pall].
Identifieur interne : 000588 ( PubMed/Curation ); précédent : 000587; suivant : 000589[Effects of cycloheximide, aminoglutethimide, indomethacin, cytochalasin B and colchicine on ovulation and the ultrastructure of the ovarian follicle wall in the stellate sturgeon Acipenser stellatus Pall].
Auteurs : O B Trubnikova [Russie]Source :
- Ontogenez [ 0475-1450 ]
English descriptors
- KwdEn :
- Aminoglutethimide (pharmacology), Animals, Colchicine (pharmacology), Cycloheximide (pharmacology), Cyclooxygenase Inhibitors (pharmacology), Cytochalasin B (pharmacology), Cytoskeleton (drug effects), Enzyme Inhibitors (pharmacology), Female, Fishes (physiology), Hormones (pharmacology), In Vitro Techniques, Indomethacin (pharmacology), Microscopy, Electron, Scanning, Microscopy, Electron, Scanning Transmission, Ovarian Follicle (drug effects), Ovarian Follicle (ultrastructure), Ovulation (drug effects), Ovulation (physiology), Protein Synthesis Inhibitors (pharmacology).
- MESH :
- chemical , pharmacology : Aminoglutethimide, Colchicine, Cycloheximide, Cyclooxygenase Inhibitors, Cytochalasin B, Enzyme Inhibitors, Hormones, Indomethacin, Protein Synthesis Inhibitors.
- drug effects : Cytoskeleton, Ovarian Follicle, Ovulation.
- physiology : Fishes, Ovulation.
- ultrastructure : Ovarian Follicle.
- Animals, Female, In Vitro Techniques, Microscopy, Electron, Scanning, Microscopy, Electron, Scanning Transmission.
Abstract
The inhibitor of protein synthesis cycloheximide, inhibitor of steroidogenesis aminoglutethimide, and inhibitor of prostaglandin synthesis indomethacin, as well as the drugs affecting the cell cytoskeleton, such as cytochalasin B and colchicine, were used for studying the mechanisms of ovulation in the stellate sturgeon Acipenser stellatus Pall. Follicles were isolated from the body cavity within certain time intervals after the injection of pituitary suspension to a female and cultivated in media with the inhibitors. In the case of follicles isolated in the middle of the period from hormonal injection until ovulation, cycloheximide, cytochalasin B, and aminoglutethimide suppressed ovulation most effectively, while in the case of oocytes isolated during the last quarter of this period, aminoglutethimide and cytochalasin B were the most effective. It was shown using TEM and SEM that cycloheximide suppressed all processes related to the preparation for ovulation, except the initial ones: contraction of follicle cells and their processes and secondary flattening of these cells. In the presence of aminoglutethimide, the follicle cells underwent pathological changes. Incubation in the media containing indomethacin and colchicine prevented degradation of the outer theca layer at the follicle apex. In the presence of cytochalasin B affecting the cytoskeleton, the drawing of follicle cell processes from the jelly coat channels was blocked, the outer theca cells were strongly contracted, but the cell layer integrity was affected and it was divided in separate fragments. A relationship is discussed between the metabolic processes and morphological changes that lead to ovulation. It was proposed on the basis of the present and previous data that the preovulatory preparation of the follicle tissues comprises two contractile and two apoptotic processes distinctly coordinated in time and space.
PubMed: 12722593
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000588
Links to Exploration step
pubmed:12722593Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Effects of cycloheximide, aminoglutethimide, indomethacin, cytochalasin B and colchicine on ovulation and the ultrastructure of the ovarian follicle wall in the stellate sturgeon Acipenser stellatus Pall].</title><author><name sortKey="Trubnikova, O B" sort="Trubnikova, O B" uniqKey="Trubnikova O" first="O B" last="Trubnikova">O B Trubnikova</name><affiliation wicri:level="1"><nlm:affiliation>Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="????"><PubDate><MedlineDate>2003 Mar-Apr</MedlineDate></PubDate></date><idno type="RBID">pubmed:12722593</idno><idno type="pmid">12722593</idno><idno type="wicri:Area/PubMed/Corpus">000588</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000588</idno><idno type="wicri:Area/PubMed/Curation">000588</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000588</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Effects of cycloheximide, aminoglutethimide, indomethacin, cytochalasin B and colchicine on ovulation and the ultrastructure of the ovarian follicle wall in the stellate sturgeon Acipenser stellatus Pall].</title><author><name sortKey="Trubnikova, O B" sort="Trubnikova, O B" uniqKey="Trubnikova O" first="O B" last="Trubnikova">O B Trubnikova</name><affiliation wicri:level="1"><nlm:affiliation>Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.</nlm:affiliation><country xml:lang="fr">Russie</country><wicri:regionArea>Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow</wicri:regionArea></affiliation></author></analytic><series><title level="j">Ontogenez</title><idno type="ISSN">0475-1450</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aminoglutethimide (pharmacology)</term><term>Animals</term><term>Colchicine (pharmacology)</term><term>Cycloheximide (pharmacology)</term><term>Cyclooxygenase Inhibitors (pharmacology)</term><term>Cytochalasin B (pharmacology)</term><term>Cytoskeleton (drug effects)</term><term>Enzyme Inhibitors (pharmacology)</term><term>Female</term><term>Fishes (physiology)</term><term>Hormones (pharmacology)</term><term>In Vitro Techniques</term><term>Indomethacin (pharmacology)</term><term>Microscopy, Electron, Scanning</term><term>Microscopy, Electron, Scanning Transmission</term><term>Ovarian Follicle (drug effects)</term><term>Ovarian Follicle (ultrastructure)</term><term>Ovulation (drug effects)</term><term>Ovulation (physiology)</term><term>Protein Synthesis Inhibitors (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Aminoglutethimide</term><term>Colchicine</term><term>Cycloheximide</term><term>Cyclooxygenase Inhibitors</term><term>Cytochalasin B</term><term>Enzyme Inhibitors</term><term>Hormones</term><term>Indomethacin</term><term>Protein Synthesis Inhibitors</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cytoskeleton</term><term>Ovarian Follicle</term><term>Ovulation</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term><term>Ovulation</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Ovarian Follicle</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>In Vitro Techniques</term><term>Microscopy, Electron, Scanning</term><term>Microscopy, Electron, Scanning Transmission</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The inhibitor of protein synthesis cycloheximide, inhibitor of steroidogenesis aminoglutethimide, and inhibitor of prostaglandin synthesis indomethacin, as well as the drugs affecting the cell cytoskeleton, such as cytochalasin B and colchicine, were used for studying the mechanisms of ovulation in the stellate sturgeon Acipenser stellatus Pall. Follicles were isolated from the body cavity within certain time intervals after the injection of pituitary suspension to a female and cultivated in media with the inhibitors. In the case of follicles isolated in the middle of the period from hormonal injection until ovulation, cycloheximide, cytochalasin B, and aminoglutethimide suppressed ovulation most effectively, while in the case of oocytes isolated during the last quarter of this period, aminoglutethimide and cytochalasin B were the most effective. It was shown using TEM and SEM that cycloheximide suppressed all processes related to the preparation for ovulation, except the initial ones: contraction of follicle cells and their processes and secondary flattening of these cells. In the presence of aminoglutethimide, the follicle cells underwent pathological changes. Incubation in the media containing indomethacin and colchicine prevented degradation of the outer theca layer at the follicle apex. In the presence of cytochalasin B affecting the cytoskeleton, the drawing of follicle cell processes from the jelly coat channels was blocked, the outer theca cells were strongly contracted, but the cell layer integrity was affected and it was divided in separate fragments. A relationship is discussed between the metabolic processes and morphological changes that lead to ovulation. It was proposed on the basis of the present and previous data that the preovulatory preparation of the follicle tissues comprises two contractile and two apoptotic processes distinctly coordinated in time and space.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12722593</PMID><DateCreated><Year>2003</Year><Month>04</Month><Day>30</Day></DateCreated><DateCompleted><Year>2003</Year><Month>08</Month><Day>12</Day></DateCompleted><DateRevised><Year>2014</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0475-1450</ISSN><JournalIssue CitedMedium="Print"><Volume>34</Volume><Issue>2</Issue><PubDate><MedlineDate>2003 Mar-Apr</MedlineDate></PubDate></JournalIssue><Title>Ontogenez</Title><ISOAbbreviation>Ontogenez</ISOAbbreviation></Journal><ArticleTitle>[Effects of cycloheximide, aminoglutethimide, indomethacin, cytochalasin B and colchicine on ovulation and the ultrastructure of the ovarian follicle wall in the stellate sturgeon Acipenser stellatus Pall].</ArticleTitle><Pagination><MedlinePgn>142-53</MedlinePgn></Pagination><Abstract><AbstractText>The inhibitor of protein synthesis cycloheximide, inhibitor of steroidogenesis aminoglutethimide, and inhibitor of prostaglandin synthesis indomethacin, as well as the drugs affecting the cell cytoskeleton, such as cytochalasin B and colchicine, were used for studying the mechanisms of ovulation in the stellate sturgeon Acipenser stellatus Pall. Follicles were isolated from the body cavity within certain time intervals after the injection of pituitary suspension to a female and cultivated in media with the inhibitors. In the case of follicles isolated in the middle of the period from hormonal injection until ovulation, cycloheximide, cytochalasin B, and aminoglutethimide suppressed ovulation most effectively, while in the case of oocytes isolated during the last quarter of this period, aminoglutethimide and cytochalasin B were the most effective. It was shown using TEM and SEM that cycloheximide suppressed all processes related to the preparation for ovulation, except the initial ones: contraction of follicle cells and their processes and secondary flattening of these cells. In the presence of aminoglutethimide, the follicle cells underwent pathological changes. Incubation in the media containing indomethacin and colchicine prevented degradation of the outer theca layer at the follicle apex. In the presence of cytochalasin B affecting the cytoskeleton, the drawing of follicle cell processes from the jelly coat channels was blocked, the outer theca cells were strongly contracted, but the cell layer integrity was affected and it was divided in separate fragments. A relationship is discussed between the metabolic processes and morphological changes that lead to ovulation. It was proposed on the basis of the present and previous data that the preovulatory preparation of the follicle tissues comprises two contractile and two apoptotic processes distinctly coordinated in time and space.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Trubnikova</LastName><ForeName>O B</ForeName><Initials>OB</Initials><AffiliationInfo><Affiliation>Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>rus</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><VernacularTitle>Vliianie tsiklogeksimida, aminogliutetimida, indometatsina, tsitokhalazina B i kolkhitsina na ovuliatsiiu i ul'trastrukturu stenki ovarial'nogo follikula sevriugi Acipenser stellatus Pall.</VernacularTitle></Article><MedlineJournalInfo><Country>Russia (Federation)</Country><MedlineTA>Ontogenez</MedlineTA><NlmUniqueID>0341527</NlmUniqueID><ISSNLinking>0475-1450</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016861">Cyclooxygenase Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004791">Enzyme Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006728">Hormones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011500">Protein Synthesis Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0O54ZQ14I9</RegistryNumber><NameOfSubstance UI="D000616">Aminoglutethimide</NameOfSubstance></Chemical><Chemical><RegistryNumber>3CHI920QS7</RegistryNumber><NameOfSubstance UI="D003571">Cytochalasin B</NameOfSubstance></Chemical><Chemical><RegistryNumber>98600C0908</RegistryNumber><NameOfSubstance UI="D003513">Cycloheximide</NameOfSubstance></Chemical><Chemical><RegistryNumber>SML2Y3J35T</RegistryNumber><NameOfSubstance UI="D003078">Colchicine</NameOfSubstance></Chemical><Chemical><RegistryNumber>XXE1CET956</RegistryNumber><NameOfSubstance UI="D007213">Indomethacin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000616" MajorTopicYN="N">Aminoglutethimide</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003078" MajorTopicYN="N">Colchicine</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003513" MajorTopicYN="N">Cycloheximide</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016861" MajorTopicYN="N">Cyclooxygenase Inhibitors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003571" MajorTopicYN="N">Cytochalasin B</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003599" MajorTopicYN="N">Cytoskeleton</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004791" MajorTopicYN="N">Enzyme Inhibitors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006728" MajorTopicYN="N">Hormones</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D066298" MajorTopicYN="N">In Vitro Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007213" MajorTopicYN="N">Indomethacin</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008855" MajorTopicYN="N">Microscopy, Electron, Scanning</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017348" MajorTopicYN="N">Microscopy, Electron, Scanning Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006080" MajorTopicYN="N">Ovarian Follicle</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010060" MajorTopicYN="N">Ovulation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011500" MajorTopicYN="N">Protein Synthesis Inhibitors</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>5</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>8</Month><Day>13</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>5</Month><Day>2</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12722593</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000588 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 000588 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:12722593
|texte= [Effects of cycloheximide, aminoglutethimide, indomethacin, cytochalasin B and colchicine on ovulation and the ultrastructure of the ovarian follicle wall in the stellate sturgeon Acipenser stellatus Pall].
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:12722593" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |