Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community.
Identifieur interne : 000327 ( PubMed/Curation ); précédent : 000326; suivant : 000328Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community.
Auteurs : Ebrahim H. Najdegerami [Belgique] ; Tiet Ngoc Tran ; Tom Defoirdt ; Massimo Marzorati ; Patrick Sorgeloos ; Nico Boon ; Peter BossierSource :
- FEMS microbiology ecology [ 1574-6941 ] ; 2012.
English descriptors
- KwdEn :
- MESH :
- chemical : Hydroxybutyrates, Polyesters.
- geographic : Siberia.
- drug effects : Bacteria.
- growth & development : Bacteria, Fishes.
- methods : Aquaculture.
- microbiology : Fishes, Gastrointestinal Tract.
- Animals, Dietary Supplements, Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
Poly-β-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2% and 5% PHB were investigated. Replacing 2% of the diet with PHB improved weight gain, specific growth rate (SGR) and survival in the sturgeon fingerlings during the 10-week experimental period. Community-level physiological profiling and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) were used to analyze the microbial community diversity and community organization in the sturgeon gastrointestinal tract. DGGE analysis revealed that PHB affected the intestinal microbial species richness and diversity. The highest species richness was observed with 2% PHB. DNA sequencing of the dominant bands in 2% and 5% PHB treatments revealed that PHB stimulated bacteria belonging to the genera Bacillus and Ruminococcaceae. Principal component analysis, Lorenz curves and the Shannon index of Biolog Ecoplate data revealed that aerobic metabolic potential of the bacterial community was different in the PHB-treated fishes as compared with the control situation. Overall, our results indicate that PHB act as microbial control agents and replacement of 2% of Siberian sturgeon fingerling diet with PHB has beneficial effects.
DOI: 10.1111/j.1574-6941.2011.01194.x
PubMed: 22066757
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Najdegerami</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, Belgium. ebrahim.hosseinnajdegerami@ugent.be</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent</wicri:regionArea></affiliation></author><author><name sortKey="Tran, Tiet Ngoc" sort="Tran, Tiet Ngoc" uniqKey="Tran T" first="Tiet Ngoc" last="Tran">Tiet Ngoc Tran</name></author><author><name sortKey="Defoirdt, Tom" sort="Defoirdt, Tom" uniqKey="Defoirdt T" first="Tom" last="Defoirdt">Tom Defoirdt</name></author><author><name sortKey="Marzorati, Massimo" sort="Marzorati, Massimo" uniqKey="Marzorati M" first="Massimo" last="Marzorati">Massimo Marzorati</name></author><author><name sortKey="Sorgeloos, Patrick" sort="Sorgeloos, Patrick" uniqKey="Sorgeloos P" first="Patrick" last="Sorgeloos">Patrick Sorgeloos</name></author><author><name sortKey="Boon, Nico" sort="Boon, Nico" uniqKey="Boon N" first="Nico" last="Boon">Nico Boon</name></author><author><name sortKey="Bossier, Peter" sort="Bossier, Peter" uniqKey="Bossier P" first="Peter" last="Bossier">Peter Bossier</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2012">2012</date><idno type="RBID">pubmed:22066757</idno><idno type="pmid">22066757</idno><idno type="doi">10.1111/j.1574-6941.2011.01194.x</idno><idno type="wicri:Area/PubMed/Corpus">000327</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000327</idno><idno type="wicri:Area/PubMed/Curation">000327</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000327</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community.</title><author><name sortKey="Najdegerami, Ebrahim H" sort="Najdegerami, Ebrahim H" uniqKey="Najdegerami E" first="Ebrahim H" last="Najdegerami">Ebrahim H. Najdegerami</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, Belgium. ebrahim.hosseinnajdegerami@ugent.be</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent</wicri:regionArea></affiliation></author><author><name sortKey="Tran, Tiet Ngoc" sort="Tran, Tiet Ngoc" uniqKey="Tran T" first="Tiet Ngoc" last="Tran">Tiet Ngoc Tran</name></author><author><name sortKey="Defoirdt, Tom" sort="Defoirdt, Tom" uniqKey="Defoirdt T" first="Tom" last="Defoirdt">Tom Defoirdt</name></author><author><name sortKey="Marzorati, Massimo" sort="Marzorati, Massimo" uniqKey="Marzorati M" first="Massimo" last="Marzorati">Massimo Marzorati</name></author><author><name sortKey="Sorgeloos, Patrick" sort="Sorgeloos, Patrick" uniqKey="Sorgeloos P" first="Patrick" last="Sorgeloos">Patrick Sorgeloos</name></author><author><name sortKey="Boon, Nico" sort="Boon, Nico" uniqKey="Boon N" first="Nico" last="Boon">Nico Boon</name></author><author><name sortKey="Bossier, Peter" sort="Bossier, Peter" uniqKey="Bossier P" first="Peter" last="Bossier">Peter Bossier</name></author></analytic><series><title level="j">FEMS microbiology ecology</title><idno type="eISSN">1574-6941</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Aquaculture (methods)</term><term>Bacteria (drug effects)</term><term>Bacteria (growth & development)</term><term>Dietary Supplements</term><term>Fishes (growth & development)</term><term>Fishes (microbiology)</term><term>Gastrointestinal Tract (microbiology)</term><term>Hydroxybutyrates</term><term>Polyesters</term><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term><term>Siberia</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Hydroxybutyrates</term><term>Polyesters</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Siberia</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Bacteria</term><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Aquaculture</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Fishes</term><term>Gastrointestinal Tract</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Dietary Supplements</term><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Poly-β-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2% and 5% PHB were investigated. Replacing 2% of the diet with PHB improved weight gain, specific growth rate (SGR) and survival in the sturgeon fingerlings during the 10-week experimental period. Community-level physiological profiling and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) were used to analyze the microbial community diversity and community organization in the sturgeon gastrointestinal tract. DGGE analysis revealed that PHB affected the intestinal microbial species richness and diversity. The highest species richness was observed with 2% PHB. DNA sequencing of the dominant bands in 2% and 5% PHB treatments revealed that PHB stimulated bacteria belonging to the genera Bacillus and Ruminococcaceae. Principal component analysis, Lorenz curves and the Shannon index of Biolog Ecoplate data revealed that aerobic metabolic potential of the bacterial community was different in the PHB-treated fishes as compared with the control situation. Overall, our results indicate that PHB act as microbial control agents and replacement of 2% of Siberian sturgeon fingerling diet with PHB has beneficial effects.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22066757</PMID><DateCreated><Year>2012</Year><Month>03</Month><Day>01</Day></DateCreated><DateCompleted><Year>2012</Year><Month>04</Month><Day>11</Day></DateCompleted><DateRevised><Year>2012</Year><Month>03</Month><Day>01</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1574-6941</ISSN><JournalIssue CitedMedium="Internet"><Volume>79</Volume><Issue>1</Issue><PubDate><Year>2012</Year><Month>Jan</Month></PubDate></JournalIssue><Title>FEMS microbiology ecology</Title><ISOAbbreviation>FEMS Microbiol. Ecol.</ISOAbbreviation></Journal><ArticleTitle>Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community.</ArticleTitle><Pagination><MedlinePgn>25-33</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1574-6941.2011.01194.x</ELocationID><Abstract><AbstractText>Poly-β-hydroxybutyrate (PHB) is a natural polymer that can be depolymerized into water-soluble short-chain fatty acid monomers. These monomers can act as microbial control agents. In this study, the effects of partially replacing the diet of Siberian sturgeon fingerlings with 2% and 5% PHB were investigated. Replacing 2% of the diet with PHB improved weight gain, specific growth rate (SGR) and survival in the sturgeon fingerlings during the 10-week experimental period. Community-level physiological profiling and PCR-denaturing gradient gel electrophoresis (PCR-DGGE) were used to analyze the microbial community diversity and community organization in the sturgeon gastrointestinal tract. DGGE analysis revealed that PHB affected the intestinal microbial species richness and diversity. The highest species richness was observed with 2% PHB. DNA sequencing of the dominant bands in 2% and 5% PHB treatments revealed that PHB stimulated bacteria belonging to the genera Bacillus and Ruminococcaceae. Principal component analysis, Lorenz curves and the Shannon index of Biolog Ecoplate data revealed that aerobic metabolic potential of the bacterial community was different in the PHB-treated fishes as compared with the control situation. Overall, our results indicate that PHB act as microbial control agents and replacement of 2% of Siberian sturgeon fingerling diet with PHB has beneficial effects.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Najdegerami</LastName><ForeName>Ebrahim H</ForeName><Initials>EH</Initials><AffiliationInfo><Affiliation>Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Ghent, Belgium. ebrahim.hosseinnajdegerami@ugent.be</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tran</LastName><ForeName>Tiet Ngoc</ForeName><Initials>TN</Initials></Author><Author ValidYN="Y"><LastName>Defoirdt</LastName><ForeName>Tom</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Marzorati</LastName><ForeName>Massimo</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Sorgeloos</LastName><ForeName>Patrick</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Boon</LastName><ForeName>Nico</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Bossier</LastName><ForeName>Peter</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEMS Microbiol Ecol</MedlineTA><NlmUniqueID>8901229</NlmUniqueID><ISSNLinking>0168-6496</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006885">Hydroxybutyrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011091">Polyesters</NameOfSubstance></Chemical><Chemical><RegistryNumber>26063-00-3</RegistryNumber><NameOfSubstance UI="C003182">poly-beta-hydroxybutyrate</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017756" MajorTopicYN="N">Aquaculture</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019587" MajorTopicYN="Y">Dietary Supplements</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D041981" MajorTopicYN="N">Gastrointestinal Tract</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006885" MajorTopicYN="Y">Hydroxybutyrates</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011091" MajorTopicYN="Y">Polyesters</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012800" MajorTopicYN="N" Type="Geographic">Siberia</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2012</Year><Month>4</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22066757</ArticleId><ArticleId IdType="doi">10.1111/j.1574-6941.2011.01194.x</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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