The effects of temperature and body size on immunological development and responsiveness in juvenile shortnose sturgeon (Acipenser brevirostrum).
Identifieur interne : 000169 ( PubMed/Curation ); précédent : 000168; suivant : 000170The effects of temperature and body size on immunological development and responsiveness in juvenile shortnose sturgeon (Acipenser brevirostrum).
Auteurs : Ana M. Gradil [Canada] ; Glenda M. Wright [Canada] ; David J. Speare [Canada] ; Dorota W. Wadowska [Canada] ; Sara Purcell [Canada] ; Mark D. Fast [Canada]Source :
- Fish & shellfish immunology [ 1095-9947 ] ; 2014.
English descriptors
- KwdEn :
- Animals, Body Size (immunology), Copepoda (physiology), Ectoparasitic Infestations (genetics), Ectoparasitic Infestations (immunology), Ectoparasitic Infestations (veterinary), Female, Fish Diseases (genetics), Fish Diseases (immunology), Fish Proteins (genetics), Fish Proteins (metabolism), Fishes (anatomy & histology), Fishes (genetics), Fishes (growth & development), Gene Expression Regulation, Immune System (anatomy & histology), Immune System (growth & development), Immune System (metabolism), Immunity, Innate, Reverse Transcriptase Polymerase Chain Reaction (veterinary), Temperature.
- MESH :
- chemical , genetics : Fish Proteins.
- anatomy & histology : Fishes, Immune System.
- genetics : Ectoparasitic Infestations, Fish Diseases, Fishes.
- growth & development : Fishes, Immune System.
- immunology : Body Size, Ectoparasitic Infestations, Fish Diseases.
- chemical , metabolism : Fish Proteins, Immune System.
- physiology : Copepoda.
- veterinary : Ectoparasitic Infestations, Reverse Transcriptase Polymerase Chain Reaction.
- Animals, Female, Gene Expression Regulation, Immunity, Innate, Temperature.
Abstract
Sturgeon are an important evolutionary taxa of which little is known regarding their responses to environmental factors. Water temperature strongly influences growth in fish; however, its effect on sturgeon immune responses is unknown. The objective of this study was to assess how 2 different temperatures affect immune responses in shortnose sturgeon (Acipenser brevirostrum) relevant immune organs such as the meningeal myeloid tissue, spleen, thymus and skin. These responses were studied in 2 different sizes of same age juvenile sturgeon kept at either 11 °C or 20 °C (4 treatment groups), before and after exposure to an ectoparasitic copepod (Dichelesthium oblongum). Based on a differential cell count, temperature was found to strongly influence immune cell production in the meningeal myeloid tissue, regardless of the fish sizes considered. Morphometric analysis of splenic white pulp showed a transient response to temperature. There were no differences between the groups in the morphometric analysis of thymus size. Splenic IRF-1 and IRF-2 had similar expression profiles, significantly higher in fish kept at 20 °C for the first 6 weeks of the study but not by 14 weeks. In the skin, IRF-1 was significantly higher in the fish kept at 11 °C over the first 6 weeks of the study. IRF-2 had a similar profile but there were no differences between the groups by the end of the trial. In conclusion, higher water temperatures (up to 20 °C) may have beneficial effects in maximizing growth and improving immunological capacity, regardless of the fish sizes considered in this study.
DOI: 10.1016/j.fsi.2014.07.036
PubMed: 25130144
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Wright</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. Electronic address: gwright@upei.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3</wicri:regionArea></affiliation></author><author><name sortKey="Speare, David J" sort="Speare, David J" uniqKey="Speare D" first="David J" last="Speare">David J. 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Gradil</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. Electronic address: anamgradil@gmail.com.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3</wicri:regionArea></affiliation></author><author><name sortKey="Wright, Glenda M" sort="Wright, Glenda M" uniqKey="Wright G" first="Glenda M" last="Wright">Glenda M. Wright</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. Electronic address: gwright@upei.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3</wicri:regionArea></affiliation></author><author><name sortKey="Speare, David J" sort="Speare, David J" uniqKey="Speare D" first="David J" last="Speare">David J. Speare</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3</wicri:regionArea></affiliation></author><author><name sortKey="Wadowska, Dorota W" sort="Wadowska, Dorota W" uniqKey="Wadowska D" first="Dorota W" last="Wadowska">Dorota W. Wadowska</name><affiliation wicri:level="1"><nlm:affiliation>Electron Microscopy Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. Electronic address: Wadowska@upei.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Electron Microscopy Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3</wicri:regionArea></affiliation></author><author><name sortKey="Purcell, Sara" sort="Purcell, Sara" uniqKey="Purcell S" first="Sara" last="Purcell">Sara Purcell</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3</wicri:regionArea></affiliation></author><author><name sortKey="Fast, Mark D" sort="Fast, Mark D" uniqKey="Fast M" first="Mark D" last="Fast">Mark D. Fast</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. Electronic address: mfast@upei.ca.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3</wicri:regionArea></affiliation></author></analytic><series><title level="j">Fish & shellfish immunology</title><idno type="eISSN">1095-9947</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Body Size (immunology)</term><term>Copepoda (physiology)</term><term>Ectoparasitic Infestations (genetics)</term><term>Ectoparasitic Infestations (immunology)</term><term>Ectoparasitic Infestations (veterinary)</term><term>Female</term><term>Fish Diseases (genetics)</term><term>Fish Diseases (immunology)</term><term>Fish Proteins (genetics)</term><term>Fish Proteins (metabolism)</term><term>Fishes (anatomy & histology)</term><term>Fishes (genetics)</term><term>Fishes (growth & development)</term><term>Gene Expression Regulation</term><term>Immune System (anatomy & histology)</term><term>Immune System (growth & development)</term><term>Immune System (metabolism)</term><term>Immunity, Innate</term><term>Reverse Transcriptase Polymerase Chain Reaction (veterinary)</term><term>Temperature</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fish Proteins</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Fishes</term><term>Immune System</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Ectoparasitic Infestations</term><term>Fish Diseases</term><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Fishes</term><term>Immune System</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Body Size</term><term>Ectoparasitic Infestations</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fish Proteins</term><term>Immune System</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Copepoda</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Ectoparasitic Infestations</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Gene Expression Regulation</term><term>Immunity, Innate</term><term>Temperature</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sturgeon are an important evolutionary taxa of which little is known regarding their responses to environmental factors. Water temperature strongly influences growth in fish; however, its effect on sturgeon immune responses is unknown. The objective of this study was to assess how 2 different temperatures affect immune responses in shortnose sturgeon (Acipenser brevirostrum) relevant immune organs such as the meningeal myeloid tissue, spleen, thymus and skin. These responses were studied in 2 different sizes of same age juvenile sturgeon kept at either 11 °C or 20 °C (4 treatment groups), before and after exposure to an ectoparasitic copepod (Dichelesthium oblongum). Based on a differential cell count, temperature was found to strongly influence immune cell production in the meningeal myeloid tissue, regardless of the fish sizes considered. Morphometric analysis of splenic white pulp showed a transient response to temperature. There were no differences between the groups in the morphometric analysis of thymus size. Splenic IRF-1 and IRF-2 had similar expression profiles, significantly higher in fish kept at 20 °C for the first 6 weeks of the study but not by 14 weeks. In the skin, IRF-1 was significantly higher in the fish kept at 11 °C over the first 6 weeks of the study. IRF-2 had a similar profile but there were no differences between the groups by the end of the trial. In conclusion, higher water temperatures (up to 20 °C) may have beneficial effects in maximizing growth and improving immunological capacity, regardless of the fish sizes considered in this study.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25130144</PMID><DateCreated><Year>2014</Year><Month>09</Month><Day>15</Day></DateCreated><DateCompleted><Year>2015</Year><Month>05</Month><Day>19</Day></DateCompleted><DateRevised><Year>2014</Year><Month>09</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1095-9947</ISSN><JournalIssue CitedMedium="Internet"><Volume>40</Volume><Issue>2</Issue><PubDate><Year>2014</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Fish & shellfish immunology</Title><ISOAbbreviation>Fish Shellfish Immunol.</ISOAbbreviation></Journal><ArticleTitle>The effects of temperature and body size on immunological development and responsiveness in juvenile shortnose sturgeon (Acipenser brevirostrum).</ArticleTitle><Pagination><MedlinePgn>545-55</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.fsi.2014.07.036</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1050-4648(14)00277-0</ELocationID><Abstract><AbstractText>Sturgeon are an important evolutionary taxa of which little is known regarding their responses to environmental factors. Water temperature strongly influences growth in fish; however, its effect on sturgeon immune responses is unknown. The objective of this study was to assess how 2 different temperatures affect immune responses in shortnose sturgeon (Acipenser brevirostrum) relevant immune organs such as the meningeal myeloid tissue, spleen, thymus and skin. These responses were studied in 2 different sizes of same age juvenile sturgeon kept at either 11 °C or 20 °C (4 treatment groups), before and after exposure to an ectoparasitic copepod (Dichelesthium oblongum). Based on a differential cell count, temperature was found to strongly influence immune cell production in the meningeal myeloid tissue, regardless of the fish sizes considered. Morphometric analysis of splenic white pulp showed a transient response to temperature. There were no differences between the groups in the morphometric analysis of thymus size. Splenic IRF-1 and IRF-2 had similar expression profiles, significantly higher in fish kept at 20 °C for the first 6 weeks of the study but not by 14 weeks. In the skin, IRF-1 was significantly higher in the fish kept at 11 °C over the first 6 weeks of the study. IRF-2 had a similar profile but there were no differences between the groups by the end of the trial. In conclusion, higher water temperatures (up to 20 °C) may have beneficial effects in maximizing growth and improving immunological capacity, regardless of the fish sizes considered in this study.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gradil</LastName><ForeName>Ana M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. Electronic address: anamgradil@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wright</LastName><ForeName>Glenda M</ForeName><Initials>GM</Initials><AffiliationInfo><Affiliation>Department of Biomedical Sciences, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. Electronic address: gwright@upei.ca.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Speare</LastName><ForeName>David J</ForeName><Initials>DJ</Initials><AffiliationInfo><Affiliation>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wadowska</LastName><ForeName>Dorota W</ForeName><Initials>DW</Initials><AffiliationInfo><Affiliation>Electron Microscopy Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. Electronic address: Wadowska@upei.ca.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Purcell</LastName><ForeName>Sara</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fast</LastName><ForeName>Mark D</ForeName><Initials>MD</Initials><AffiliationInfo><Affiliation>Department of Pathology and Microbiology, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island C1A 4P3, Canada. Electronic address: mfast@upei.ca.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>08</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Fish Shellfish Immunol</MedlineTA><NlmUniqueID>9505220</NlmUniqueID><ISSNLinking>1050-4648</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D029941">Fish Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049628" MajorTopicYN="N">Body Size</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D033342" MajorTopicYN="N">Copepoda</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004478" MajorTopicYN="N">Ectoparasitic Infestations</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000662" MajorTopicYN="Y">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005393" MajorTopicYN="N">Fish Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029941" MajorTopicYN="N">Fish Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="Y">Fishes</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005786" MajorTopicYN="Y">Gene Expression Regulation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007107" MajorTopicYN="N">Immune System</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000662" MajorTopicYN="N">veterinary</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="Y">Temperature</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Immunity</Keyword><Keyword MajorTopicYN="N">Size</Keyword><Keyword MajorTopicYN="N">Sturgeon</Keyword><Keyword MajorTopicYN="N">Temperature</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>01</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>07</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>07</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>8</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>8</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>5</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25130144</ArticleId><ArticleId IdType="pii">S1050-4648(14)00277-0</ArticleId><ArticleId IdType="doi">10.1016/j.fsi.2014.07.036</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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