Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen-Thawed Persian Sturgeon (Acipenser persicus) Sperm.
Identifieur interne : 000054 ( PubMed/Curation ); précédent : 000053; suivant : 000055Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen-Thawed Persian Sturgeon (Acipenser persicus) Sperm.
Auteurs : M S Aramli [Iran] ; K. Golshahi [Iran] ; R M Nazari [Iran] ; A. Golpour [République tchèque] ; S. Aramli [Iran]Source :
- Reproduction in domestic animals = Zuchthygiene [ 1439-0531 ] ; 2016.
Abstract
Amino acids have an important biological role for the prevention of cell damage during cryopreservation. The aim of this study was to investigate the effects of glutamine on post-thaw sperm motility and fertilization success in the Persian sturgeon (Acipenser persicus). Sperm collected from six fish was cryopreserved in extenders containing different glutamine concentrations (2.5, 5 and 10 mm). Sperm samples diluted at the ratio of 1 : 1 using the extenders were subjected to cryopreservation. After dilution, the sperm suspensions were sucked into 250-μl straws; the straws were placed on the tray, frozen in nitrogen vapour and plunged into liquid nitrogen. Then, sperm were thawed in a water bath at 40°C for 5 s and used for analysis. Our results revealed that an increase in the concentration of glutamine caused a significant increase in the motility percentage, curvilinear velocity (VCL) and also fertilization success in the Persian sturgeon (p < 0.05). Comparing all concentrations of glutamine, the best concentration for sperm motility and fertilization rate was 10 mm. In addition, higher post-thaw motility percentage, VCL, and fertilization and hatching rates were obtained with the extender at the concentration of 10 mm (p < 0.05). The findings of this study showed that glutamine was of greater benefit to Persian sturgeon sperm motility during frozen-thawed process.
DOI: 10.1111/rda.12704
PubMed: 27168189
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000054
Links to Exploration step
pubmed:27168189Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen-Thawed Persian Sturgeon (Acipenser persicus) Sperm.</title><author><name sortKey="Aramli, M S" sort="Aramli, M S" uniqKey="Aramli M" first="M S" last="Aramli">M S Aramli</name><affiliation wicri:level="1"><nlm:affiliation>Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia</wicri:regionArea></affiliation></author><author><name sortKey="Golshahi, K" sort="Golshahi, K" uniqKey="Golshahi K" first="K" last="Golshahi">K. Golshahi</name><affiliation wicri:level="1"><nlm:affiliation>Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran</wicri:regionArea></affiliation></author><author><name sortKey="Nazari, R M" sort="Nazari, R M" uniqKey="Nazari R" first="R M" last="Nazari">R M Nazari</name><affiliation wicri:level="1"><nlm:affiliation>Rajaee Sturgeon Propagation Center, Sari, Mazandaran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Rajaee Sturgeon Propagation Center, Sari, Mazandaran</wicri:regionArea></affiliation></author><author><name sortKey="Golpour, A" sort="Golpour, A" uniqKey="Golpour A" first="A" last="Golpour">A. Golpour</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea></affiliation></author><author><name sortKey="Aramli, S" sort="Aramli, S" uniqKey="Aramli S" first="S" last="Aramli">S. Aramli</name><affiliation wicri:level="1"><nlm:affiliation>Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27168189</idno><idno type="pmid">27168189</idno><idno type="doi">10.1111/rda.12704</idno><idno type="wicri:Area/PubMed/Corpus">000054</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000054</idno><idno type="wicri:Area/PubMed/Curation">000054</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000054</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen-Thawed Persian Sturgeon (Acipenser persicus) Sperm.</title><author><name sortKey="Aramli, M S" sort="Aramli, M S" uniqKey="Aramli M" first="M S" last="Aramli">M S Aramli</name><affiliation wicri:level="1"><nlm:affiliation>Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia</wicri:regionArea></affiliation></author><author><name sortKey="Golshahi, K" sort="Golshahi, K" uniqKey="Golshahi K" first="K" last="Golshahi">K. Golshahi</name><affiliation wicri:level="1"><nlm:affiliation>Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran</wicri:regionArea></affiliation></author><author><name sortKey="Nazari, R M" sort="Nazari, R M" uniqKey="Nazari R" first="R M" last="Nazari">R M Nazari</name><affiliation wicri:level="1"><nlm:affiliation>Rajaee Sturgeon Propagation Center, Sari, Mazandaran, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Rajaee Sturgeon Propagation Center, Sari, Mazandaran</wicri:regionArea></affiliation></author><author><name sortKey="Golpour, A" sort="Golpour, A" uniqKey="Golpour A" first="A" last="Golpour">A. Golpour</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic.</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany</wicri:regionArea></affiliation></author><author><name sortKey="Aramli, S" sort="Aramli, S" uniqKey="Aramli S" first="S" last="Aramli">S. Aramli</name><affiliation wicri:level="1"><nlm:affiliation>Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil, Iran.</nlm:affiliation><country xml:lang="fr">Iran</country><wicri:regionArea>Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil</wicri:regionArea></affiliation></author></analytic><series><title level="j">Reproduction in domestic animals = Zuchthygiene</title><idno type="eISSN">1439-0531</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Amino acids have an important biological role for the prevention of cell damage during cryopreservation. The aim of this study was to investigate the effects of glutamine on post-thaw sperm motility and fertilization success in the Persian sturgeon (Acipenser persicus). Sperm collected from six fish was cryopreserved in extenders containing different glutamine concentrations (2.5, 5 and 10 mm). Sperm samples diluted at the ratio of 1 : 1 using the extenders were subjected to cryopreservation. After dilution, the sperm suspensions were sucked into 250-μl straws; the straws were placed on the tray, frozen in nitrogen vapour and plunged into liquid nitrogen. Then, sperm were thawed in a water bath at 40°C for 5 s and used for analysis. Our results revealed that an increase in the concentration of glutamine caused a significant increase in the motility percentage, curvilinear velocity (VCL) and also fertilization success in the Persian sturgeon (p < 0.05). Comparing all concentrations of glutamine, the best concentration for sperm motility and fertilization rate was 10 mm. In addition, higher post-thaw motility percentage, VCL, and fertilization and hatching rates were obtained with the extender at the concentration of 10 mm (p < 0.05). The findings of this study showed that glutamine was of greater benefit to Persian sturgeon sperm motility during frozen-thawed process.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">27168189</PMID><DateCreated><Year>2016</Year><Month>07</Month><Day>09</Day></DateCreated><DateRevised><Year>2016</Year><Month>12</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1439-0531</ISSN><JournalIssue CitedMedium="Internet"><Volume>51</Volume><Issue>4</Issue><PubDate><Year>2016</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Reproduction in domestic animals = Zuchthygiene</Title><ISOAbbreviation>Reprod. Domest. Anim.</ISOAbbreviation></Journal><ArticleTitle>Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen-Thawed Persian Sturgeon (Acipenser persicus) Sperm.</ArticleTitle><Pagination><MedlinePgn>474-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/rda.12704</ELocationID><Abstract><AbstractText>Amino acids have an important biological role for the prevention of cell damage during cryopreservation. The aim of this study was to investigate the effects of glutamine on post-thaw sperm motility and fertilization success in the Persian sturgeon (Acipenser persicus). Sperm collected from six fish was cryopreserved in extenders containing different glutamine concentrations (2.5, 5 and 10 mm). Sperm samples diluted at the ratio of 1 : 1 using the extenders were subjected to cryopreservation. After dilution, the sperm suspensions were sucked into 250-μl straws; the straws were placed on the tray, frozen in nitrogen vapour and plunged into liquid nitrogen. Then, sperm were thawed in a water bath at 40°C for 5 s and used for analysis. Our results revealed that an increase in the concentration of glutamine caused a significant increase in the motility percentage, curvilinear velocity (VCL) and also fertilization success in the Persian sturgeon (p < 0.05). Comparing all concentrations of glutamine, the best concentration for sperm motility and fertilization rate was 10 mm. In addition, higher post-thaw motility percentage, VCL, and fertilization and hatching rates were obtained with the extender at the concentration of 10 mm (p < 0.05). The findings of this study showed that glutamine was of greater benefit to Persian sturgeon sperm motility during frozen-thawed process.</AbstractText><CopyrightInformation>© 2016 Blackwell Verlag GmbH.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Aramli</LastName><ForeName>M S</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Fisheries Department, Faculty of Natural Resources, Urmia University, Urmia, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Golshahi</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Young Researchers and Elites Club, North Tehran Branch, Islamic Azad University, Tehran, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nazari</LastName><ForeName>R M</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>Rajaee Sturgeon Propagation Center, Sari, Mazandaran, Iran.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Golpour</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Research Institute of Fish Culture and Hydrobiology, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Vodnany, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aramli</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Medicine Laboratory, Alavi Educational and Treatment Center, Ardabil University of Medical Sciences, Ardabil, Iran.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>05</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Reprod Domest Anim</MedlineTA><NlmUniqueID>9015668</NlmUniqueID><ISSNLinking>0936-6768</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>01</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>04</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">27168189</ArticleId><ArticleId IdType="doi">10.1111/rda.12704</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000054 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd -nk 000054 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= PubMed
|étape= Curation
|type= RBID
|clé= pubmed:27168189
|texte= Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen-Thawed Persian Sturgeon (Acipenser persicus) Sperm.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Curation/RBID.i -Sk "pubmed:27168189" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Curation/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |