Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon (Acipenser stellatus Pall.) III. Polymorphism.
Identifieur interne : 000782 ( PubMed/Corpus ); précédent : 000781; suivant : 000783Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon (Acipenser stellatus Pall.) III. Polymorphism.
Auteurs : A A Kuznetzov ; B F Goncharov ; E. Burzawa-GerardSource :
- General and comparative endocrinology [ 0016-6480 ] ; 1983.
English descriptors
- KwdEn :
- Animals, Biological Assay, Bufonidae, Chromatography, DEAE-Cellulose, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Female, Fishes (metabolism), Gonadotropins, Pituitary (isolation & purification), Gonadotropins, Pituitary (pharmacology), Isoelectric Focusing, Male, Oocytes (drug effects), Oocytes (growth & development), Pituitary Gland (analysis), Polymorphism, Genetic.
- MESH :
- chemical , isolation & purification : Gonadotropins, Pituitary.
- analysis : Pituitary Gland.
- drug effects : Oocytes.
- growth & development : Oocytes.
- metabolism : Fishes.
- chemical , pharmacology : Gonadotropins, Pituitary.
- Animals, Biological Assay, Bufonidae, Chromatography, DEAE-Cellulose, Chromatography, Gel, Electrophoresis, Polyacrylamide Gel, Female, Isoelectric Focusing, Male, Polymorphism, Genetic.
Abstract
Four biologically active fractions of gonadotropic hormone (aci-GTH-A, -B, -C, -D) were isolated and purified from acetonized pituitaries of the starred sturgeon (Acipenser stellatus Pall.). Their separation was achieved by DEAE-cellulose chromatography. Disc-electrophoresis and especially isoelectric focusing in polyacrylamide gel showed that each fraction contained several components. Not less than 15 different components as a whole with isoelectric points ranging from 4.5 to 7.0 could be counted in four aci-GTH preparations. All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. N-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with N-terminal glycine. No differences in the spectra of aci-GTH isoforms were found when pituitary extract, newly purified or 3 years older hormone preparations were submitted to isoelectric focusing.
PubMed: 6840529
Links to Exploration step
pubmed:6840529Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon (Acipenser stellatus Pall.) III. Polymorphism.</title>
<author><name sortKey="Kuznetzov, A A" sort="Kuznetzov, A A" uniqKey="Kuznetzov A" first="A A" last="Kuznetzov">A A Kuznetzov</name>
</author>
<author><name sortKey="Goncharov, B F" sort="Goncharov, B F" uniqKey="Goncharov B" first="B F" last="Goncharov">B F Goncharov</name>
</author>
<author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E" last="Burzawa-Gerard">E. Burzawa-Gerard</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1983">1983</date>
<idno type="RBID">pubmed:6840529</idno>
<idno type="pmid">6840529</idno>
<idno type="wicri:Area/PubMed/Corpus">000782</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000782</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon (Acipenser stellatus Pall.) III. Polymorphism.</title>
<author><name sortKey="Kuznetzov, A A" sort="Kuznetzov, A A" uniqKey="Kuznetzov A" first="A A" last="Kuznetzov">A A Kuznetzov</name>
</author>
<author><name sortKey="Goncharov, B F" sort="Goncharov, B F" uniqKey="Goncharov B" first="B F" last="Goncharov">B F Goncharov</name>
</author>
<author><name sortKey="Burzawa Gerard, E" sort="Burzawa Gerard, E" uniqKey="Burzawa Gerard E" first="E" last="Burzawa-Gerard">E. Burzawa-Gerard</name>
</author>
</analytic>
<series><title level="j">General and comparative endocrinology</title>
<idno type="ISSN">0016-6480</idno>
<imprint><date when="1983" type="published">1983</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Biological Assay</term>
<term>Bufonidae</term>
<term>Chromatography, DEAE-Cellulose</term>
<term>Chromatography, Gel</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Female</term>
<term>Fishes (metabolism)</term>
<term>Gonadotropins, Pituitary (isolation & purification)</term>
<term>Gonadotropins, Pituitary (pharmacology)</term>
<term>Isoelectric Focusing</term>
<term>Male</term>
<term>Oocytes (drug effects)</term>
<term>Oocytes (growth & development)</term>
<term>Pituitary Gland (analysis)</term>
<term>Polymorphism, Genetic</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Gonadotropins, Pituitary</term>
</keywords>
<keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Pituitary Gland</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Oocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Oocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Gonadotropins, Pituitary</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Biological Assay</term>
<term>Bufonidae</term>
<term>Chromatography, DEAE-Cellulose</term>
<term>Chromatography, Gel</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Female</term>
<term>Isoelectric Focusing</term>
<term>Male</term>
<term>Polymorphism, Genetic</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Four biologically active fractions of gonadotropic hormone (aci-GTH-A, -B, -C, -D) were isolated and purified from acetonized pituitaries of the starred sturgeon (Acipenser stellatus Pall.). Their separation was achieved by DEAE-cellulose chromatography. Disc-electrophoresis and especially isoelectric focusing in polyacrylamide gel showed that each fraction contained several components. Not less than 15 different components as a whole with isoelectric points ranging from 4.5 to 7.0 could be counted in four aci-GTH preparations. All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. N-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with N-terminal glycine. No differences in the spectra of aci-GTH isoforms were found when pituitary extract, newly purified or 3 years older hormone preparations were submitted to isoelectric focusing.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">6840529</PMID>
<DateCreated><Year>1983</Year>
<Month>06</Month>
<Day>10</Day>
</DateCreated>
<DateCompleted><Year>1983</Year>
<Month>06</Month>
<Day>10</Day>
</DateCompleted>
<DateRevised><Year>2006</Year>
<Month>11</Month>
<Day>15</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0016-6480</ISSN>
<JournalIssue CitedMedium="Print"><Volume>49</Volume>
<Issue>3</Issue>
<PubDate><Year>1983</Year>
<Month>Mar</Month>
</PubDate>
</JournalIssue>
<Title>General and comparative endocrinology</Title>
<ISOAbbreviation>Gen. Comp. Endocrinol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon (Acipenser stellatus Pall.) III. Polymorphism.</ArticleTitle>
<Pagination><MedlinePgn>364-74</MedlinePgn>
</Pagination>
<Abstract><AbstractText>Four biologically active fractions of gonadotropic hormone (aci-GTH-A, -B, -C, -D) were isolated and purified from acetonized pituitaries of the starred sturgeon (Acipenser stellatus Pall.). Their separation was achieved by DEAE-cellulose chromatography. Disc-electrophoresis and especially isoelectric focusing in polyacrylamide gel showed that each fraction contained several components. Not less than 15 different components as a whole with isoelectric points ranging from 4.5 to 7.0 could be counted in four aci-GTH preparations. All these components were active in toad oocyte maturation test. Only two of four preparations (aci-GTH-A and -D) were practically free of common components. All aci-GTH preparations were shown to be homogeneous and identical by molecular weight, sedimentation coefficient, sialic acid content, and some immunological properties. N-terminal amino acid analysis revealed tyrosine and leucine in all aci-GTH preparations, with the only exception of aci-GTH-D that contained an additional polypeptide with N-terminal glycine. No differences in the spectra of aci-GTH isoforms were found when pituitary extract, newly purified or 3 years older hormone preparations were submitted to isoelectric focusing.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kuznetzov</LastName>
<ForeName>A A</ForeName>
<Initials>AA</Initials>
</Author>
<Author ValidYN="Y"><LastName>Goncharov</LastName>
<ForeName>B F</ForeName>
<Initials>BF</Initials>
</Author>
<Author ValidYN="Y"><LastName>Burzawa-Gerard</LastName>
<ForeName>E</ForeName>
<Initials>E</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Gen Comp Endocrinol</MedlineTA>
<NlmUniqueID>0370735</NlmUniqueID>
<ISSNLinking>0016-6480</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006065">Gonadotropins, Pituitary</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001681" MajorTopicYN="N">Biological Assay</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002025" MajorTopicYN="N">Bufonidae</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002848" MajorTopicYN="N">Chromatography, DEAE-Cellulose</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002850" MajorTopicYN="N">Chromatography, Gel</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006065" MajorTopicYN="N">Gonadotropins, Pituitary</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
<QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007525" MajorTopicYN="N">Isoelectric Focusing</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009865" MajorTopicYN="N">Oocytes</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010902" MajorTopicYN="N">Pituitary Gland</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011110" MajorTopicYN="N">Polymorphism, Genetic</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1983</Year>
<Month>3</Month>
<Day>1</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>1983</Year>
<Month>3</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>1</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>1983</Year>
<Month>3</Month>
<Day>1</Day>
<Hour>0</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">6840529</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000782 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000782 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Eau |area= EsturgeonV1 |flux= PubMed |étape= Corpus |type= RBID |clé= pubmed:6840529 |texte= Pituitary gonadotropic hormone from a chondrostean fish, starred sturgeon (Acipenser stellatus Pall.) III. Polymorphism. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:6840529" \ | HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \ | NlmPubMed2Wicri -a EsturgeonV1
![]() | This area was generated with Dilib version V0.6.27. | ![]() |