Immunohistochemical identification of the cytoskeletal elements in the notochord cells of bony fishes.
Identifieur interne : 000670 ( PubMed/Corpus ); précédent : 000669; suivant : 000671Immunohistochemical identification of the cytoskeletal elements in the notochord cells of bony fishes.
Auteurs : R J SchmitzSource :
- Journal of morphology [ 0362-2525 ] ; 1998.
English descriptors
- KwdEn :
- MESH :
- chemical , immunology : Keratins.
- chemical : Antibodies, Monoclonal.
- anatomy & histology : Fishes, Perches.
- chemistry : Cytoskeleton, Notochord.
- cytology : Notochord.
- metabolism : Fishes, Perches.
- Animals, Humans, Immunohistochemistry.
Abstract
The medulla of the unconstricted notochords of the shortnose sturgeon, Acipenser brevirostratus, and African lungfish, Protopterus annectens, and the cellular component of the intervertebral joint tissue of the teleost fish, Perca flavescens, are comprised of cells with a large central vacuole. Previous studies on the fine structure of this tissue revealed that the cytoplasm surrounding these vacuoles consists of 10-nm-diameter intermediate filaments. Since in mammals there are a large number of tissue-specific types of intermediate filaments, this study uses antibodies to mammalian intermediate filaments to determine the type of filaments present in the notochord cells of bony fishes. Positive labeling using a polyclonal antibody to human skin keratins is observed in the cytoplasm of the notochord cells in the intervertebral tissues of Perca. These tissues are also probed with the AE series antibodies that label keratins found in mammalian epithelial cells. In both Protopterus and Acipenser the peripheral cytoplasm of the notochord cells is labeled with all three AE antibodies. In Perca only the AE3 antibody probe produces positive staining. These staining patterns are consistent with previous studies on the localization of cytokeratins in fish tissues and indicate that the intermediate filaments in the notochord cells of bony fishes are immunologically similar to the mammalian keratins.
DOI: 10.1002/(SICI)1097-4687(199805)236:2<105::AID-JMOR2>3.0.CO;2-4
PubMed: 9549086
Links to Exploration step
pubmed:9549086Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Immunohistochemical identification of the cytoskeletal elements in the notochord cells of bony fishes.</title><author><name sortKey="Schmitz, R J" sort="Schmitz, R J" uniqKey="Schmitz R" first="R J" last="Schmitz">R J Schmitz</name><affiliation><nlm:affiliation>Department of Biology, University of Wisconsin Stevens Point 54481, USA. rschmitz@uwsp.edu</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:9549086</idno><idno type="pmid">9549086</idno><idno type="doi">10.1002/(SICI)1097-4687(199805)236:2<105::AID-JMOR2>3.0.CO;2-4</idno><idno type="wicri:Area/PubMed/Corpus">000670</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000670</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Immunohistochemical identification of the cytoskeletal elements in the notochord cells of bony fishes.</title><author><name sortKey="Schmitz, R J" sort="Schmitz, R J" uniqKey="Schmitz R" first="R J" last="Schmitz">R J Schmitz</name><affiliation><nlm:affiliation>Department of Biology, University of Wisconsin Stevens Point 54481, USA. rschmitz@uwsp.edu</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of morphology</title><idno type="ISSN">0362-2525</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Monoclonal</term><term>Cytoskeleton (chemistry)</term><term>Fishes (anatomy & histology)</term><term>Fishes (metabolism)</term><term>Humans</term><term>Immunohistochemistry</term><term>Keratins (immunology)</term><term>Notochord (chemistry)</term><term>Notochord (cytology)</term><term>Perches (anatomy & histology)</term><term>Perches (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Keratins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Fishes</term><term>Perches</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cytoskeleton</term><term>Notochord</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Notochord</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term><term>Perches</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Immunohistochemistry</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The medulla of the unconstricted notochords of the shortnose sturgeon, Acipenser brevirostratus, and African lungfish, Protopterus annectens, and the cellular component of the intervertebral joint tissue of the teleost fish, Perca flavescens, are comprised of cells with a large central vacuole. Previous studies on the fine structure of this tissue revealed that the cytoplasm surrounding these vacuoles consists of 10-nm-diameter intermediate filaments. Since in mammals there are a large number of tissue-specific types of intermediate filaments, this study uses antibodies to mammalian intermediate filaments to determine the type of filaments present in the notochord cells of bony fishes. Positive labeling using a polyclonal antibody to human skin keratins is observed in the cytoplasm of the notochord cells in the intervertebral tissues of Perca. These tissues are also probed with the AE series antibodies that label keratins found in mammalian epithelial cells. In both Protopterus and Acipenser the peripheral cytoplasm of the notochord cells is labeled with all three AE antibodies. In Perca only the AE3 antibody probe produces positive staining. These staining patterns are consistent with previous studies on the localization of cytokeratins in fish tissues and indicate that the intermediate filaments in the notochord cells of bony fishes are immunologically similar to the mammalian keratins.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9549086</PMID><DateCreated><Year>1998</Year><Month>05</Month><Day>13</Day></DateCreated><DateCompleted><Year>1998</Year><Month>05</Month><Day>13</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0362-2525</ISSN><JournalIssue CitedMedium="Print"><Volume>236</Volume><Issue>2</Issue><PubDate><Year>1998</Year><Month>May</Month></PubDate></JournalIssue><Title>Journal of morphology</Title><ISOAbbreviation>J. Morphol.</ISOAbbreviation></Journal><ArticleTitle>Immunohistochemical identification of the cytoskeletal elements in the notochord cells of bony fishes.</ArticleTitle><Pagination><MedlinePgn>105-16</MedlinePgn></Pagination><Abstract><AbstractText>The medulla of the unconstricted notochords of the shortnose sturgeon, Acipenser brevirostratus, and African lungfish, Protopterus annectens, and the cellular component of the intervertebral joint tissue of the teleost fish, Perca flavescens, are comprised of cells with a large central vacuole. Previous studies on the fine structure of this tissue revealed that the cytoplasm surrounding these vacuoles consists of 10-nm-diameter intermediate filaments. Since in mammals there are a large number of tissue-specific types of intermediate filaments, this study uses antibodies to mammalian intermediate filaments to determine the type of filaments present in the notochord cells of bony fishes. Positive labeling using a polyclonal antibody to human skin keratins is observed in the cytoplasm of the notochord cells in the intervertebral tissues of Perca. These tissues are also probed with the AE series antibodies that label keratins found in mammalian epithelial cells. In both Protopterus and Acipenser the peripheral cytoplasm of the notochord cells is labeled with all three AE antibodies. In Perca only the AE3 antibody probe produces positive staining. These staining patterns are consistent with previous studies on the localization of cytokeratins in fish tissues and indicate that the intermediate filaments in the notochord cells of bony fishes are immunologically similar to the mammalian keratins.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Schmitz</LastName><ForeName>R J</ForeName><Initials>RJ</Initials><AffiliationInfo><Affiliation>Department of Biology, University of Wisconsin Stevens Point 54481, USA. rschmitz@uwsp.edu</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Morphol</MedlineTA><NlmUniqueID>0406125</NlmUniqueID><ISSNLinking>0022-2887</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>68238-35-7</RegistryNumber><NameOfSubstance UI="D007633">Keratins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000911" MajorTopicYN="N">Antibodies, Monoclonal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003599" MajorTopicYN="N">Cytoskeleton</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="Y">anatomy & histology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007150" MajorTopicYN="N">Immunohistochemistry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007633" MajorTopicYN="N">Keratins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009672" MajorTopicYN="N">Notochord</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000166" MajorTopicYN="Y">cytology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010471" MajorTopicYN="N">Perches</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="Y">anatomy & histology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>25</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2000</Year><Month>6</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1998</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>25</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9549086</ArticleId><ArticleId IdType="pii">10.1002/(SICI)1097-4687(199805)236:2<105::AID-JMOR2>3.0.CO;2-4</ArticleId><ArticleId IdType="doi">10.1002/(SICI)1097-4687(199805)236:2<105::AID-JMOR2>3.0.CO;2-4</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Eau/explor/EsturgeonV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000670 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000670 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Eau
|area= EsturgeonV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:9549086
|texte= Immunohistochemical identification of the cytoskeletal elements in the notochord cells of bony fishes.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:9549086" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a EsturgeonV1
| This area was generated with Dilib version V0.6.27. |  |