Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system.
Identifieur interne : 000576 ( PubMed/Corpus ); précédent : 000575; suivant : 000577Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system.
Auteurs : L. Kuznetsova ; A. Shpakov ; Yu Rusakov ; S. Plesneva ; V. Bondareva ; M. PertsevaSource :
- Regulatory peptides [ 0167-0115 ] ; 2003.
English descriptors
- KwdEn :
- Adenylyl Cyclases (metabolism), Animals, Fishes, Insulin (chemistry), Insulin (pharmacology), Lampreys, Mollusca, Muscle, Skeletal (drug effects), Muscle, Skeletal (enzymology), Muscle, Smooth (drug effects), Muscle, Smooth (enzymology), Rats, Receptor, Insulin (metabolism), Signal Transduction (drug effects), Swine.
- MESH :
- chemical , chemistry : Insulin.
- chemical , metabolism : Adenylyl Cyclases, Receptor, Insulin.
- chemical , pharmacology : Insulin.
- drug effects : Muscle, Skeletal, Muscle, Smooth, Signal Transduction.
- enzymology : Muscle, Skeletal, Muscle, Smooth.
- Animals, Fishes, Lampreys, Mollusca, Rats, Swine.
Abstract
The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC(50), i.e. concentration leading to half-maximal activating effect of the hormone (10(-11)-10(-7) M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.
PubMed: 14599718
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system.</title><author><name sortKey="Kuznetsova, L" sort="Kuznetsova, L" uniqKey="Kuznetsova L" first="L" last="Kuznetsova">L. Kuznetsova</name><affiliation><nlm:affiliation>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr. 44, 194223, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Shpakov, A" sort="Shpakov, A" uniqKey="Shpakov A" first="A" last="Shpakov">A. Shpakov</name></author><author><name sortKey="Rusakov, Yu" sort="Rusakov, Yu" uniqKey="Rusakov Y" first="Yu" last="Rusakov">Yu Rusakov</name></author><author><name sortKey="Plesneva, S" sort="Plesneva, S" uniqKey="Plesneva S" first="S" last="Plesneva">S. Plesneva</name></author><author><name sortKey="Bondareva, V" sort="Bondareva, V" uniqKey="Bondareva V" first="V" last="Bondareva">V. Bondareva</name></author><author><name sortKey="Pertseva, M" sort="Pertseva, M" uniqKey="Pertseva M" first="M" last="Pertseva">M. Pertseva</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:14599718</idno><idno type="pmid">14599718</idno><idno type="wicri:Area/PubMed/Corpus">000576</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000576</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system.</title><author><name sortKey="Kuznetsova, L" sort="Kuznetsova, L" uniqKey="Kuznetsova L" first="L" last="Kuznetsova">L. Kuznetsova</name><affiliation><nlm:affiliation>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr. 44, 194223, St. Petersburg, Russia.</nlm:affiliation></affiliation></author><author><name sortKey="Shpakov, A" sort="Shpakov, A" uniqKey="Shpakov A" first="A" last="Shpakov">A. Shpakov</name></author><author><name sortKey="Rusakov, Yu" sort="Rusakov, Yu" uniqKey="Rusakov Y" first="Yu" last="Rusakov">Yu Rusakov</name></author><author><name sortKey="Plesneva, S" sort="Plesneva, S" uniqKey="Plesneva S" first="S" last="Plesneva">S. Plesneva</name></author><author><name sortKey="Bondareva, V" sort="Bondareva, V" uniqKey="Bondareva V" first="V" last="Bondareva">V. Bondareva</name></author><author><name sortKey="Pertseva, M" sort="Pertseva, M" uniqKey="Pertseva M" first="M" last="Pertseva">M. Pertseva</name></author></analytic><series><title level="j">Regulatory peptides</title><idno type="ISSN">0167-0115</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenylyl Cyclases (metabolism)</term><term>Animals</term><term>Fishes</term><term>Insulin (chemistry)</term><term>Insulin (pharmacology)</term><term>Lampreys</term><term>Mollusca</term><term>Muscle, Skeletal (drug effects)</term><term>Muscle, Skeletal (enzymology)</term><term>Muscle, Smooth (drug effects)</term><term>Muscle, Smooth (enzymology)</term><term>Rats</term><term>Receptor, Insulin (metabolism)</term><term>Signal Transduction (drug effects)</term><term>Swine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Insulin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adenylyl Cyclases</term><term>Receptor, Insulin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Insulin</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Muscle, Skeletal</term><term>Muscle, Smooth</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Muscle, Skeletal</term><term>Muscle, Smooth</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Fishes</term><term>Lampreys</term><term>Mollusca</term><term>Rats</term><term>Swine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC(50), i.e. concentration leading to half-maximal activating effect of the hormone (10(-11)-10(-7) M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14599718</PMID><DateCreated><Year>2003</Year><Month>11</Month><Day>05</Day></DateCreated><DateCompleted><Year>2004</Year><Month>08</Month><Day>06</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0167-0115</ISSN><JournalIssue CitedMedium="Print"><Volume>116</Volume><Issue>1-3</Issue><PubDate><Year>2003</Year><Month>Nov</Month><Day>15</Day></PubDate></JournalIssue><Title>Regulatory peptides</Title><ISOAbbreviation>Regul. Pept.</ISOAbbreviation></Journal><ArticleTitle>Comparative study of biological activity of insulins of lower vertebrates in the novel adenylyl cyclase test-system.</ArticleTitle><Pagination><MedlinePgn>81-6</MedlinePgn></Pagination><Abstract><AbstractText>The biological activity of insulins of lower vertebrates (teleosts-Oncorhynchus gorbuscha, Scorpaena porcus, chondrosteans-Acipenser guldenstaedti and cyclostomates-Lamperta fluviatilis) was studied and compared with that of standard pig insulin. The determination of biological activity was made using the novel adenylyl cyclase (AC) test-system based on the adenylyl cyclase signaling mechanism (ACSM) of insulin action discovered earlier by the authors. The biological activity of insulins was estimated as EC(50), i.e. concentration leading to half-maximal activating effect of the hormone (10(-11)-10(-7) M), in vitro, on adenylyl cyclase in two types of the target tissues: in membrane fractions of the muscles of rat and mollusc Anodonta cygnea. In rat, the efficiency of insulins was found to decrease in the following order: pig insulin>scorpaena insulin>gorbuscha insulin>sturgeon insulin>lamprey insulin. In the mollusc, the order was different: sturgeon insulin>scorpaena insulin>pig insulin>gorbuscha insulin. Lamprey insulin at the same concentrations did not apparently reach the maximal adenylyl cyclase activating effect. The suggestion was made that differences in the biological activity of insulins depend on the hormone structure and a number of indexes characteristic of the adenylyl cyclase test-system in the vertebrate and invertebrate tissues. The proposed adenylyl cyclase test-system is highly sensitive to insulin at physiological concentrations, has good reproduction and is easy to apply.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kuznetsova</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, Thorez pr. 44, 194223, St. Petersburg, Russia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shpakov</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Rusakov</LastName><ForeName>Yu</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Plesneva</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Bondareva</LastName><ForeName>V</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Pertseva</LastName><ForeName>M</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Regul Pept</MedlineTA><NlmUniqueID>8100479</NlmUniqueID><ISSNLinking>0167-0115</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007328">Insulin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.10.1</RegistryNumber><NameOfSubstance UI="D011972">Receptor, Insulin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.6.1.1</RegistryNumber><NameOfSubstance UI="D000262">Adenylyl Cyclases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000262" MajorTopicYN="N">Adenylyl Cyclases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005399" MajorTopicYN="N">Fishes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007328" MajorTopicYN="N">Insulin</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007798" MajorTopicYN="N">Lampreys</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008974" MajorTopicYN="N">Mollusca</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018482" MajorTopicYN="N">Muscle, Skeletal</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009130" MajorTopicYN="N">Muscle, Smooth</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D051381" MajorTopicYN="N">Rats</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011972" MajorTopicYN="N">Receptor, Insulin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015398" MajorTopicYN="N">Signal Transduction</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013552" MajorTopicYN="N">Swine</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>11</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>8</Month><Day>7</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>11</Month><Day>6</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14599718</ArticleId><ArticleId IdType="pii">S0167011503001964</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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