Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii.
Identifieur interne : 000375 ( PubMed/Corpus ); précédent : 000374; suivant : 000376Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii.
Auteurs : Zhi-Hua Li ; Ping Li ; Marek Rodina ; Tomas RandakSource :
- Aquatic toxicology (Amsterdam, Netherlands) [ 1879-1514 ] ; 2010.
English descriptors
- KwdEn :
- Animals, Cadmium (toxicity), Calcium Channel Blockers (pharmacology), Fishes (metabolism), Fishes (physiology), Glutathione Peroxidase (metabolism), Glutathione Reductase (metabolism), Lipid Peroxidation (drug effects), Male, Oxidative Stress, Spermatozoa (drug effects), Spermatozoa (metabolism), Superoxide Dismutase (metabolism), Verapamil (pharmacology), Water Pollutants, Chemical (toxicity).
- MESH :
- chemical , metabolism : Glutathione Peroxidase, Glutathione Reductase, Superoxide Dismutase.
- chemical , pharmacology : Calcium Channel Blockers, Verapamil.
- chemical , toxicity : Cadmium, Water Pollutants, Chemical.
- drug effects : Lipid Peroxidation, Spermatozoa.
- metabolism : Fishes, Spermatozoa.
- physiology : Fishes.
- Animals, Male, Oxidative Stress.
Abstract
In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2h at 50μg/L VRP, 5.0μg/L Cd, the mixture of 50μg/L VRP+5.0μg/L Cd, 50μg/L Cd and the mixture of 50μg/L VRP+50μg/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0μg/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon.
DOI: 10.1016/j.aquatox.2010.08.007
PubMed: 20832874
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pubmed:20832874Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii.</title><author><name sortKey="Li, Zhi Hua" sort="Li, Zhi Hua" uniqKey="Li Z" first="Zhi-Hua" last="Li">Zhi-Hua Li</name><affiliation><nlm:affiliation>University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic. zhihuali06@yahoo.com</nlm:affiliation></affiliation></author><author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name></author><author><name sortKey="Randak, Tomas" sort="Randak, Tomas" uniqKey="Randak T" first="Tomas" last="Randak">Tomas Randak</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20832874</idno><idno type="pmid">20832874</idno><idno type="doi">10.1016/j.aquatox.2010.08.007</idno><idno type="wicri:Area/PubMed/Corpus">000375</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000375</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii.</title><author><name sortKey="Li, Zhi Hua" sort="Li, Zhi Hua" uniqKey="Li Z" first="Zhi-Hua" last="Li">Zhi-Hua Li</name><affiliation><nlm:affiliation>University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic. zhihuali06@yahoo.com</nlm:affiliation></affiliation></author><author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name></author><author><name sortKey="Rodina, Marek" sort="Rodina, Marek" uniqKey="Rodina M" first="Marek" last="Rodina">Marek Rodina</name></author><author><name sortKey="Randak, Tomas" sort="Randak, Tomas" uniqKey="Randak T" first="Tomas" last="Randak">Tomas Randak</name></author></analytic><series><title level="j">Aquatic toxicology (Amsterdam, Netherlands)</title><idno type="eISSN">1879-1514</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cadmium (toxicity)</term><term>Calcium Channel Blockers (pharmacology)</term><term>Fishes (metabolism)</term><term>Fishes (physiology)</term><term>Glutathione Peroxidase (metabolism)</term><term>Glutathione Reductase (metabolism)</term><term>Lipid Peroxidation (drug effects)</term><term>Male</term><term>Oxidative Stress</term><term>Spermatozoa (drug effects)</term><term>Spermatozoa (metabolism)</term><term>Superoxide Dismutase (metabolism)</term><term>Verapamil (pharmacology)</term><term>Water Pollutants, Chemical (toxicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione Peroxidase</term><term>Glutathione Reductase</term><term>Superoxide Dismutase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Calcium Channel Blockers</term><term>Verapamil</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cadmium</term><term>Water Pollutants, Chemical</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lipid Peroxidation</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fishes</term><term>Spermatozoa</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Oxidative Stress</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2h at 50μg/L VRP, 5.0μg/L Cd, the mixture of 50μg/L VRP+5.0μg/L Cd, 50μg/L Cd and the mixture of 50μg/L VRP+50μg/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0μg/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20832874</PMID><DateCreated><Year>2010</Year><Month>10</Month><Day>12</Day></DateCreated><DateCompleted><Year>2010</Year><Month>12</Month><Day>07</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1514</ISSN><JournalIssue CitedMedium="Internet"><Volume>100</Volume><Issue>4</Issue><PubDate><Year>2010</Year><Month>Nov</Month><Day>15</Day></PubDate></JournalIssue><Title>Aquatic toxicology (Amsterdam, Netherlands)</Title><ISOAbbreviation>Aquat. Toxicol.</ISOAbbreviation></Journal><ArticleTitle>Evaluating the function of calcium antagonist on the Cd-induced stress in sperm of Russian sturgeon, Acipenser gueldenstaedtii.</ArticleTitle><Pagination><MedlinePgn>373-5</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.aquatox.2010.08.007</ELocationID><Abstract><AbstractText>In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2h at 50μg/L VRP, 5.0μg/L Cd, the mixture of 50μg/L VRP+5.0μg/L Cd, 50μg/L Cd and the mixture of 50μg/L VRP+50μg/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0μg/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon.</AbstractText><CopyrightInformation>Copyright © 2010 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Zhi-Hua</ForeName><Initials>ZH</Initials><AffiliationInfo><Affiliation>University of South Bohemia in Ceske Budejovice, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodnany, Czech Republic. zhihuali06@yahoo.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Ping</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Rodina</LastName><ForeName>Marek</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Randak</LastName><ForeName>Tomas</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate 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